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The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer

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Chinese Journal of Cancer 2016年第7期35卷 374-381页

作者： xiao-Jing Wang Ying Xiong Ze-Biao Ma jian-chuan xia Yan-Fang Li Sun Yat-Sen University Cancer Center State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center of Cancer Medicine Guangzhou 510060Guangdong P.R.China Department of Gynecologic Oncology Sun Yat-Sen University Cancer CenterGuangzhou 510060GuangdongP.R. China Department of Biotherapy Sun Yat-Sen University Cancer CenterGuangzhou 510060GuangdongP.R. China

Background:Protein tyrosine kinase 6(PTK6) is overexpressed in many epithelial tumors and predicts poor ***,PTK6 expression status and its role in cervical squamous cell cancer are *** study aimed to investigate the expression level and clinical significance of PTK6 in early-stage cervical squamous cell ***:Quantitative reverse transcription-polymerase chain reaction(qRT-PCR) and western blotting analysis were performed to detect PTK6 mRNA and protein expression levels in 10 freshly frozen,early-stage cervical squamous cell cancer specimens and adjacent non-tumorous cervical *** expression of PTK6 was detected using immunohistochemical staining in 150 formalin-fixed,paraffin-embedded,early-stage cervical squamous cell cancer sections and 10 normal cervical tissue ***:The mRNA and protein levels of PTK6 in cancer tissues were higher than those in adjacent non-tumorous cervical *** analysis showed that PTK6 was not expressed in normal cervical tissues but was overexpressed in the cytoplasm of cervical squamous cell cancer *** level of PTK6 expression was significantly associated with tumor grade(P = 0.020).The 5-year overall survival rate of patients with high PTK6 expression was lower than that of patients with low PTK6 expression(81.3%vs.96.2%,P = 0.008).Multivariate Cox regression analysis showed that the expression level of PTK6 in cervical squamous cell cancer was an independent prognostic factor for patient survival(hazard ratio = 5.999,95%confidence interval 1.622-22.191,P< 0.05).Conclusions:PTK6 is overexpressed in cervical squamous ceil *** PTK6 expression is associated with reduced 5-year overall ***6 expression is an independent prognostic predictor for cervical cancer.

关键词：蛋白酪氨酸激酶鳞状细胞癌子宫颈预后早期反转录聚合酶链反应免疫组化染色组织切片

Tripartite *** 3(TRIM3)inhibits tumor growth and metastasis of liver cancer

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Chinese Journal of Cancer 2017年第9期36卷 407-419页

作者： Xu.Qiong Huang xiao.Fei Zhang Jin.Hua xia Jie Chao Qiu.Zhong Pan Jing.Jing Zhao Zi.Qi Zhou Chang.Long Chen Yan Tang De.Sheng Weng jian.Hua Zhang jian.chuan xia Department of Biotherapy State Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Epidemiology and Health Statistics Guangdong Pharmaceutical UniversityGuangzhou 510010GuangdongP.R.China Guangzhou Health Science College Guangzhou 510925GuangdongP.R.China Shanxi Entry-Exit Inspection and Quarantine Bureau Xi’an 710068ShanxiP.R.China.

Background:Reduced expression of tripartite motif-containing 3(TRIM3) has been reported to be involved in the pathogenesis of human *** our previous research,we found that TRIM3 expression was markedly reduced in human primary hepatocellular carcinoma(HCC) tissues and that low TRIM3 expression was associated with short survival of HCC ***,the role of TRIM3 in liver cancer remains *** study aimed to investigate the function of TRIM3 in liver cancer ***:The protein levels of TRIM3 in five liver cancer cell lines(SK-Hep1,Hep3 B,Huh7,HepG2,Bel-7402) and one normal liver cell line(L02) were detected with Western ***2 and Bel-7402 cells with low TRIM3 expression were infected with recombinant lentiviruses overexpressing TRIM3(LV-TRIM3),whereas Huh7 and Hep3 B cells with high TRIM3 expression were transfected with TRIM3-targeted small interfering RNA(siTRIM3).The functions of TRIM3 in the proliferation,colony formation,cell cycle,migration,invasion,and apoptosis of the above cell lines were *** effect ofTRIM3 on tumor growth and metastases in nude mice was also ***:TRIM3 was overexpressed in HepG2 and Bel-7402 cells with LV-TRIM3 infection,which further reduced proliferation,colony formation,migration,and invasion of both cell *** cycle analysis showed that TRIM3 overexpression induced G_0/G_1 phase arrest in HepG2 and Bel-7402 ***,apoptosis was not increased in HepG2 or Bel-7402 cells overexpressing ***,silencing TRIM3 expression in Huh7 and Hep3 B cells by siTRIM3 led to significantly decreased percentages of both cells in the G_0/G_1 phase and promoted cell proliferation,colony formation,migration,and *** vivo experiment results confirmed that TRIM3 overexpression suppressed tumor growth and ***:TRIM3 plays a tumor-suppressing role in the regulation of liver cancer development by reducing cell proliferation through cell cycle arrest a

关键词： Tripartite motif-containing 3 (TRIM3) Liver cancer Tumor suppressor Cell cycle

High expression level of EDIL3 in HCC predicts poor prognosis of HCC patients

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World Journal of Gastroenterology 2010年第36期16卷 4611-4615页

作者： jian-Cong Sun,xiao-Ting Liang,Ke Pan,Hui Wang,Jing-Jing Zhao,jian-Jun Li,Hai-Qing Ma,Yi-Bing Chen,jian-chuan xia,State Key Laboratory of Oncology in South China,Cancer Center,Sun Yat-Sen University,Guangzhou 510060,Guangdong Province,China State Key Laboratory of Oncology in Southern China Cancer Center Sun Yat-Sen University 651 Dongfeng East Road Guangzhou 510060 Guangdong Province China. xiajch@***

AIM:To determine the role of epidermal growth factor-like repeats and discoidin I-like domains 3(EDIL3)in pathogenesis of hepatocellular carcinoma(HCC)by investigating the EDIL3 expression in HCC and its prognostic value for ***:EDIL3 expression was detected in 101 HCC surgical tissue samples with immunohistochemistry method,and its relation with clinicopathologic features and prognosis of HCC patients was ***:EDIL3 was highly expressed in 48.5%of the HCC *** the EDIL3 expression level did not correlate with any clinicopathological parameters,KaplanMeier survival analysis showed that high expression level of EDIL3 resulted in a significantly poor prognosis of HCC patients(log-rank test,P=0.010).Multivariate Cox's analysis showed that the EDIL3 expression level was a significant and independent prognostic parameter for the overall survival rate of HCC patients(hazard ratio=1.978,95%confidence interval =1.139-3.435,P=0.015).CONCLUSION:High expression level of EDIL3 pre-dicts poor prognosis of HCC ***3 may be a potential target of antiangiogenic therapy for HCC.

关键词： Epidermal growth factor-like repeats and discoidin I-like domains 3 Hepatocellular carcinoma Prognosis Angiogenesis

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丝印原色油墨颜料选择方法研究

包装工程 2006年第6期27卷 40-42页

作者：简川霞广东工业大学广州510000

通过对丝印三原色油墨光谱特性、密度特性以及颜色特性的分析,提出了丝印原色颜料选择的设想和依据,并以黄墨为例进行颜料选择。

关键词：原色密度光谱曲线

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手机玻璃屏表面缺陷视觉检测方法研究

包装工程 2018年第5期39卷 16-21页

作者：简川霞高健广东工业大学广州510000

目的针对手机玻璃屏表面缺陷人工检测存在的准确率低、稳定性差等问题,提出一种基于机器视觉技术的手机玻璃屏表面缺陷检测方法。方法采用统计平均法建立模板图像,以减少外界光照对模板图像的灰度影响。采用基于互信息的配准方法实现模... 详细信息

目的针对手机玻璃屏表面缺陷人工检测存在的准确率低、稳定性差等问题,提出一种基于机器视觉技术的手机玻璃屏表面缺陷检测方法。方法采用统计平均法建立模板图像,以减少外界光照对模板图像的灰度影响。采用基于互信息的配准方法实现模板图像和待测图像的像素对齐,将配准后的待测图像与模板图像进行差分运算,获取残差图像,并采用Niblack方法实现残差图像上的缺陷判断。通过搭建的实验平台获取了300幅手机玻璃屏图像,并采用文中提出的方法、模板匹配法和人工检测法对300幅图像实施缺陷检测。结果实验结果显示,文中方法的真正率为92%,真负率为96.5%和准确率为95%。与模板匹配法和人工检测法相比,文中方法在真正率、真负率和准确率上分别至少提高了5%,4%和4.3%。结论文中方法与人工检测方法相比,提高了手机玻璃屏表面缺陷检测的准确率和稳定性。

关键词：手机玻璃屏缺陷检测差影法模板

Complications of high intensity focused ultrasound in patients with recurrent and metastatic abdominal tumors

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World Journal of Gastroenterology 2007年第19期13卷 2747-2751页

作者： jian-Jun Li Guo-Liang Xu Mo-Fa Gu Guang-Yu Luo Zhang Rong Pei-Hong Wu jian-chuan xia Department of HIFU Cancer Center Sun Yat-sen University National Key Laboratory of Oncology Guangzhou 510060 Guangdong Province China Department of Radiation Oncology Cancer Center Sun Yat-sen University National Key Laboratory of Oncology Guangzhou 510060 Guangdong Province China Pei-Hong Wu Department of Radiology Cancer Center Sun Yatsen University National Key Laboratory of Oncology Guangzhou 510060 Guangdong Province China jian-chuan xia Biotherapy Center Cancer Center Sun Yat-sen University National Key Laboratory of Oncology Guangzhou 510060 Guangdong Province China

AIM： To analyze the local and systemic complications of high intensity focused ultrasound （HIFU） for patients with recurrent and metastatic abdominal tumors. METHODS： From Aug 2001 to Aug 2004, 17 patients with recurrent and metastatic abdominal tumors were enrolled in this study. Real-time sonography was taken, and vital signs, liver and kidney function, skin burns, local reactions, and systemic effects were observed and recored before, during, and after HIFU. CT and MR/were also taken before and after HIFU. RESULTS： All 17 patients had skin burns and pain in the treatment region; the next common complication was neurapraxia of the stomach and intestines to variable degrees. The other local and systemic complications were relatively rare. Severe complications were present in two patients; one developed a superior mesenteric artery infarction resulting in necrosis of the entire small intestines, and the other one suffered from a perforation in terminal ileum due to HIFU treatment. CONCLUSION： Although HIFU is a one of noninvasive treatments for the recurrent and metastatic abdominal tumors, there are still some common and severe complications which need serious consideration.

关键词： Abdominal cavity Recurrent Metastatic Tumor High intensity focused ultrasound Ultrasonic therapy

Prognostic value of soluble MICA levels in the serum of patients with advanced hepatocellular carcinoma

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Chinese Journal of Cancer 2013年第3期32卷 141-148页

作者： jian-Jun Li Ke Pan Mo-Fa Gu Min-Shan Chen Jing-Jing Zhao Hui Wang xiao-Ting Liang jian-Cong Sun jian-chuan xia State Key Laboratory of Oncology in South China Department of Experimental Research Department of Radiation Oncology Department of Hepatobilary Oncology Sun Yat-sen University Cancer Center

Serum levels of soluble MHC class I-related chain A (sMICA) are related with the prognosis of various types of cancer; however, few studies on the prognostic value of sMICA in hepatocellular carcinoma (HCC) have been reported. In this study, we retrospectively investigated the relationship between sMICA levels and clinical features of advanced HCC, and we assessed the prognostic value of sMICA in advanced HCC. Furthermore, the relationship of serum sMICA levels and natural killer group 2, member D (NKG2D) expression on natural killer (NK) cells was also evaluated. We detected sMICA levels in the serum of 60 advanced HCC patients using enzyme-linked immunosorbent assay (ELISA) and measured expression levels of NKG2D on NK cells using flow cytometry. We found that serum sMICA levels in HCC patients were in the range of 0.10 -6.21 ng/mL. Chi-square analyses showed that sMICA level was significantly related with only tumor size. Survival analysis showed that a high sMICA level was significantly related with poor prognosis among HCC patients. Multivariate analyses indicated that sMICA was an independent prognostic factor. In addition, the levels of CD56+NKG2D+NK cells were within the range of 11.2%-55.4%, and correlation analyses indicated that sMICA level was negatively correlated with the level of NKG2D+NK cells. Our results suggest that serum sMICA levels may be an independent prognostic factor for advanced HCC.

关键词：细胞水平可溶性预后血清肝癌晚期患者价值

Effect of anti-asthma Chinese medicine chuankezhi on the anti-tumor activity of cytokine-induced killer cells

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Chinese Journal of Cancer 2013年第10期32卷 553-560页

作者： Jing-Jing Zhao Ke Pan Qi-Jing Wang Zheng-Di Xu De-Sheng Weng jian-Jun Li Yong-Qiang Li jian-chuan xia State Key Laboratory of Oncology in South China Department of Experimental Research Sun Yat-sen University Cancer Center Department of Biotherapy Center Sun Yat-sen University Cancer Center Guangzhou ImVin Pharmaceuticals Co. Ltd

chuankezhi(CKZ),a new Chinese medicine,plays an important role in *** killer(CIK)cells have been commonly used for immunotherapy in recent *** this study,we aimed to investigate the immunoregulatory effect of CKZ on CIK *** blood monocytes were isolated from healthy donors,and CIK cells were generated by culturing monocytes with interferon-gamma(IFN-γ)and interleukin *** concentrations of CKZ were added on day *** incubation for 14days in culture,the antitumor effects of CIK cells were measured by cytotoxicity *** cytometry was used to explore the effect of CKZ on CIK cell immunophenotype,intracellular cytokine production,and *** effect of CKZ on the antitumor activity of CIK cells in nude mice was also *** increased the percentage of CD3+CD56+CIK cells but did not significantly change the percentage of CD4+,CD8+,or CD4+CD25+CIK ***-conditioned CIK cells showed a greater ability to kill tumor cells,as well as a higher frequency of IFN-γand TNF-αproduction,compared with the CIK cells in the control *** also suppressed the apoptosis of CIK cells in ***,CKZ combined with CIK cells had a stronger suppressive effect on tumor growth in vivo than the CIK,CKZ,or normal saline control *** results indicate that CKZ enhances the antitumor activity of CIK cells and is a potential medicine for tumor immunotherapy.

关键词：抗肿瘤活性杀伤细胞中国医药细胞因子外周血单核细胞抗哮喘免疫调节作用 IFN-γ

IDO和CD4^+ CD25^+调节性T细胞在肿瘤免疫逃逸中相互作用的研究进展

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癌症 2009年第2期28卷 221-224页

作者：王慧潘科夏建川华南肿瘤学国家重点实验室广东广州510060 中山大学肿瘤防治中心实验研究部广东广州510060

肿瘤可以通过多种方式逃逸机体免疫系统的监控。吲哚胺-2,3双加氧酶(indoleamine-2,3-dioxygenase,IDO)和CD4+CD25+调节性T细胞(CD4+ CD25+ regulatory T cells,Tregs)是近些年在肿瘤免疫逃逸领域中备受关注的两个重要因素。两者相互作... 详细信息

肿瘤可以通过多种方式逃逸机体免疫系统的监控。吲哚胺-2,3双加氧酶(indoleamine-2,3-dioxygenase,IDO)和CD4+CD25+调节性T细胞(CD4+ CD25+ regulatory T cells,Tregs)是近些年在肿瘤免疫逃逸领域中备受关注的两个重要因素。两者相互作用在肿瘤免疫逃逸机制中起着尤为关键的作用,已引起众多研究者的关注。本文就IDO及Tregs在肿瘤免疫逃逸中的作用以及两者之间的相互关系等方面的研究进展作一综述。

关键词：吲哚胺-2,3双加氧酶 T细胞肿瘤免疫逃逸