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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第9期9卷 1167-1175页

作者： jian-ye ZHENG An-li wu Guangzhou Metropolis Construction Commission Guangzhou 510030 China College of Civil Engineering and Architecture lnner Mongolia University of Technology Hohhot 010051 China

Mesoscopic characteristics of a clayey soil specimen subjected to macroscopic loading are examined using a medi- cal-use computerized tomography (CT) instrument. Disturbed state concept (DSC) theory is based on the utilization of the hard- ening model. DSC indirectly describes material behavior by claiming that the actual response of the material is expressed in terms of the relative intact (RI) response and the fully adjusted (FA) response. The occurrence of mesoscopic structural changes of material has similarities with the occurrence of a macroscopic response of the material under loadings. In general, the relative changing value of a softening material is three to five times more than that of a hardening material. Whether special zones exist or not in a specimen cross section does not affect the following conclusion: hardening material and softening material show me- chanical differences with CT statistical indices values prominently changing, and the change is related to the superposing of a disturbance factor. A new disturbance factor evolution function is proposed. Thus, mesoscopic statistical indices are introduced to describe macroscopic behavior through the new evolution function. An application of the new evolution function proves the effectiveness of the amalgamation of a macroscopic and a mesoscopic experimental phenomenon measurement methods.

关键词： Constitutive model Disturbed state concept (DSC) Computerized tomography (CT) Softening

Research on novel multi-layer and multi-polarized slot-coupling planar antenna array

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Journal of Systems Engineering and Electronics 2009年第2期20卷 297-303页

作者： ZhangHou wu Wenzhou Wang jian Missile Inst. of Air Force Engineering Univ. Sanyuan 713800 P. R. China

A novel multi-layer planar antenna array to achieve multi-polarized radiation is developed. U-shaped coupling slots are embedded in the ground plane to extend the bandwidth. The phase relation between adjacent elements in the radiation field is analyzed when adjacent elements are fed in opposite phase. Return loss and radiation pattern are measured for a 16-element antenna array at 12.5 GHz. The radiation pattern shows a good agreement with the calculated one in the shape of the main beam. The return-loss of the proposed antenna array is less than -20 dB in the 12.5 GHz frequency band （12.25-12.75 GHz）. Because of two feed ports the antenna can transmit arbitrary elliptic polarized waves if the two feed ports have different amplitude and phase. The main factors such as element spacing, substrate medium and manufacturing imperfection are analyzed and the corresponding conclusions are presented.

关键词： planar antenna multi-polarized slot-coupling cross polarization

Local scour by multiple slit-type energy dissipaters

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Journal of Hydrodynamics 2020年第6期32卷 1221-1224页

作者： Li Yao jian-hua wu College of Water Conservancy and Ecological Engineering Nanchang Institute of TechnologyNanchang330099China College of Water Conservancy and Hydropower Engineering Hohai UniversityNanjing210098China

Energy dissipation and scour control are all the key issues for the design of hydraulic *** the basis of the high energy dissipation for the multiple slit-type energy dissipaters(M-STED)developed by the authors,in this work,the characteristics of the scour hole for the M-STED were experimentally investigated through three sets of those physical models with five cases and a scour hole form index was *** results show that,the M-STED results in not only the high energy dissipation but also the mild upstream slope of the scour hole thanks to the scour hole form index of larger than 0.5,which is in favor of the safety of the release works.

关键词： Energy dissipation scour scour hole form index multiple slit-type energy dissipater

Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress

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Journal of Integrative Agriculture 2016年第9期15卷 2097-2104页

作者： TIAN Qing-song WANG Shu-yan DU jian-cai wu Zhi-juan LI Xiao-quan HAN bing Institute of Grassland Research Chinese Academy of Agricultural Sciences Hohhot 010010 P.R. China gronomy College Inner Mongolia Agricultural University Hohhot 010010 P.R. China College of Life Science Inner Mongolia Agricultural University Hohhot 010010 P.R. China

Reference genes, stably expressing in different tissues and cells, are commonly used as the references in expression analysis. Selecting the optimum reference gene is crucial to the success of experiments. In this study, the expression stabilities of nine common reference genes, including ACT2, 18 S r RNA, APRT, EF-1α, RNA POL II, TUBα, TUBβ, GAPDH and TLF of Agropyron mongolicum, were studied under drought condition. Among them, 18 S r RNA was found to be the most optimum reference gene under drought stress by the analyzing of ge Norm and Norm Finder software. Quantitative expression levels of P5 CS using 18 S r RNA as the reference gene, and proline contents under drought stress in A. mongolicum were further operated, and we found the expression level of P5 CS gene and proline content had a significantly positive relationship（R^2=0.7763, P〈0.05）. This study established and validated 18 S r RNA as the reference genes in A. mongolicum under drought stress, providing a powerful tool for the quantitative expression analysis of drought genes in A. mongolicum.

关键词： reference genes quantitative real-time PCR drought stress proline pyrroline-5-carboxylic acid synthetase Agropyron mongolicum

Secondary genomic findings in the 2020 China Neonatal Genomes Project participants

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World Journal of Pediatrics 2022年第10期18卷 687-694页

作者： Hui Xiao jian-Tao Zhang Xin-Ran Dong Yu-Lan Lu bing-bing wu Hui-Jun Wang Zheng-Yan Zhao Lin Yang Wen-Hao Zhou Department of Neonatology Children’s Hospital of Fudan UniversityShanghaiChina Center for Molecular Medicine Children’s Hospital of Fudan UniversityShanghaiChina Department of Child Health Care Children’s HospitalZhejiang University School of MedicineHangzhouChina Department of Pediatric Endocrinology and Inherited Metabolic Diseases Children’s Hospital of Fudan UniversityShanghaiChina

Background During next generation sequencing(NGS)data interpretation in critically ill newborns,there is a potential for recognizing and reporting secondary findings(SFs).Early awareness of SFs may provide clues for disease *** this study,we assessed the frequency of SFs in the China Neonatal Genomes Project(CNGP)*** A total of 2020 clinical exome sequencing(CES)datasets were screened for variants from a list of 59 genes recommended by the American College of Medical Genetics and Genomics(ACMG)for secondary findings reporting v2.0(ACMG SF v2.0).Identified variants were classified according to the evidence-based guidelines reached by a joint consensus of the ACMG and the Association for Molecular Pathology(AMP).Results Among the 2020 CES datasets,we identified 23 ACMG-reportable genes in 61 individuals,resulting in an overall frequency of SFs at 3.02%.A total of 53 unique variants were identified,including 35 pathogenic and 18 likely pathogenic *** common disease categories of SFs associated were cardiovascular and cancer *** SF results affected the medical management and follow-up strategy in 49(80.3%)*** We presented the frequency of SFs and their impact on clinical management strategies in CNGP *** study demonstrated that SFs have important practical value in disease prevention and intervention at an early stage.

关键词： Genetics secondary findings Neonate Next generation sequencing

Two-stent strategy for renal artery stenosis with bifurcation lesion

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第8期11卷 561-567页

作者： Hong-bing YAN Bin ZHENG Zheng wu jian WANG Han-jun ZHAO Li SONG Yun-peng CHI Department of Cardiology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China

Renal artery stenosis(RAS) with a bifurcation lesion is a challenge for interventional *** aim of this study is to summarize our experience in RAS with a bifurcation *** patients with RAS involving bifurcation lesion are *** cases 1 to 3,a single-stent strategy was first ***,these three patients were converted to a two-stent strategy for bailout stent implantation in the side *** cases 4 and 5,a simultaneous kissing stent technique was *** showed that the reference vascular diameter of the main branch was much larger than those of the side *** obvious residual stenosis existed in cases 1 to 3 after stent implantation,no obvious residual stenosis was seen in cases 4 and *** artery duplex sonography was performed in cases 1 through 5 at 6,7,7,8,and 6 months,respectively,after the *** evidence of restenosis or occlusion was *** conclusion,stent implantation with the simultaneous kissing stent technique may result in more simple and more satisfactory immediate angiographic results.

关键词： Peripheral atherosclerotic artery disease Angioplasty Stents Renal artery stenosis

Studies on cDNA Probe Detection Technology for Apple Stem Pitting Virus in Kuala Pear

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Agricultural Sciences in China 2007年第1期6卷 38-46页

作者： NIU jian-xin ZHU Jun QIN Wei-ming MA bing-gang WANG Xiao-bing wu Zhong-hua Department of Horticulture Agricultural College Shihezi University Shihezi 832003 P.R.China Key Laboratory of Oasis Ecology Agriculture of Xinjiang bingtuan Shihezi 832003 P.R. China

Using Kuala pear leaves and cortexes as materials, the total RNA was extracted using two methods and these two methods were compared. The most suited methods for Kuala pear were screened; and biotin-labeled cDNA probe was synthesized using RT-PCR. The main factors that affected the sensitivity of hybridization were studied. Studies indicated that the highest sensitivity was obtained under the following conditions： probe concentration 400 ng mL^-1, formamide concentration 45%, temperature 42℃, hybridization time 6 hours. The best hybridization results were obtained when the nitrocellulose membrane purchased from Gelman was used. Better blocking of hybridization was obtained using Tween 20 compared with albumin in bovine. The detection of the total RNA using different tissues and different extraction methods was compared. This study indicates that the total RNA of fresh leaf, old leaf, cortexes, and frozen leaf showed signs of hybridization using the two extraction methods.

关键词： Kuala pear apple stem pitting virus (ASPV) cDNA probe

Unified Model for Generation Complex Networks with Utility Preferential Attachment

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Communications in Theoretical Physics 2006年第1X期46卷 183-186页

作者： wu jian-Jun State Key Loboratory of Rail Traffic Control and Safety Beijing Jiaotong University Beijing 100044 China School of Traffic and Transportation Beijing Jiaotong University Beijing 100044 China

In this paper, based on the utility preferential attachment, we propose a new unified model to generate different network topologies such as scale-free, small-world and random networks. Moreover, a new network structure named super scale network is found, which has monopoly characteristic in our simulation experiments. Finally, the characteristics of this new network are given.

关键词： generation complex networks utility preferential attachment

Bilinear Bäcklund Transformation for a Variable-Coefficient Kadomtsev–Petviashvili Equation

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Chinese Physics Letters 2011年第6期28卷 24-27页

作者： wu jian-Ping Institute of Electronic Technology Information Engineering UniversityZhengzhou 450004

Resorting to the Hirota bilinear form,a bilinear Bäcklund transformation(BT)is obtained for a variable-coefficient Kadomtsev–Petviashvili *** applications,based on the resulting bilinear BT,single-soliton solutions and two-soliton solutions together with their soliton characteristics are presented for the ***,starting from the bilinear BT,a Lax pair and a new variable-coefficient(2+1)-dimensional nonlinear evolution equation is derived.

关键词： equation bilinear soliton

Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

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Frontiers of Environmental Science & Engineering 2022年第1期16卷 77-89页

作者： jian Lu Jun wu jianhua Wang CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research(YIC)Chinese Academy of Sciences(CAS)Shandong Key Laboratory of Coastal Environmental ProcessesYICCASYantai 264003China Yantai Research Institute Harbin Engineering UniversityYantai 264006China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Microplastics existing widely in different matrices have been regarded as a reservoir for emerging *** systems have been observed to host microplastics and antibiotic resistance genes(ARGs).However,more information on proliferation of ARGs and metal resistance genes(MRGs)in mariculture system at the presence of microplastics is *** study used metagenomic analysis to investigate the distribution of ARGs and MRGs in water and microplastics of a typical mariculture *** 18 types including 174 subtypes of ARGs were detected with the total relative abundances of 1.22/1.25 copies per 16S rRNA copy for microplastics/*** resistance genes were the dominant ARGs with the abundance of 0.35/0.42 copies per 16S rRNA copy for microplastics/*** intI1 was dominant gene among 6 detected mobile genetic elements(MGEs)with the abundance of 75.46/68.70 copies per 16S rRNA copy for water/*** 9 types including 46 subtypes of MRGs were detected with total abundance of 5.02×10^(2)/6.39×10^(2)copies per 16S rRNA copy for water/microplastics while genes resistant to copper and iron served as the dominant ***,Bacteroidetes,and Actinobacteria accounted for 84.2%/89.5%of total microbial community in microplastics/*** with relatively high abundance were significantly positively related to major genera,MGEs,and *** in mariculture system could enrich most of MRGs and some ARGs to serve as potential reservoir for these *** findings of this study will provide important information on resistance gene pollution at presence of microplastics in the mariculture system for further proposing suitable strategy of environmental management.

关键词： Antibiotic resistance genes Metal resistance genes Metagenomic analysis Microplastics Mariculture