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The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis

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Asian Journal of Andrology 2015年第6期17卷 948-953,I0008-I0009页

作者： Guo-Lian Ding Ye Liu Miao-E Liu Jie-Xue Pan Meng-Xi Guo jian-zhong sheng He-Feng Huang International Peace Maternity and Child Health Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200030 China The Key Laboratory of Reproductive Genetics Ministry of Education (Zhejiang University) Hangzhou 310058 China Department of Pathology and Pathophysiology School of MedicineZhejiang University Hangzhou 310058 China

The effects of diabetes mellitus include long-term damages, dysfunctions, and failures of various organs. An important complication of diabetes is the disturbance in the male reproductive system. Glucose metabolism is an important event in spermatogenesis. Moreover, glucose metabolism is also important for maintaining basic cell activity, as well as specific functions, such as motility and fertilization ability in mature sperm. Diabetic disease and experimentally induced diabetes both demonstrated that either type 1 diabetes or type 2 diabetes could have detrimental effects on male fertility, especially on sperm quality, such as sperm motility, sperm DNA integrity, and ingredients of seminal plasma. Epigenetic modifications are essential during spermatogenesis. The epigenetic regulation represents chromatin modifications including DNA methylation, histone modifications, remodeling of nucleosomes and the higher-order chromatin reorganization and noncoding RNAs. If spermatogenesis is affected during the critical developmental window, embryonic gonadal development, and germline differentiation, environmentally-induced epigenetic modifications may become permanent in the germ line epigenome and have a potential impact on subsequent generations through epigenetic transgenerational inheritance. Diabetes may influence the epigenetic modification during sperm spermatogenesis and that these epigenetic dysregulation may be inherited through the male germ line and passed onto more than one generation, which in turn may increase the risk of diabetes in offspring.

关键词： diabetes epigenetic regulation sperm spermatogenesis

Association between gestational anemia in different trimesters and neonatal outcomes:a retrospective longitudinal cohort study

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World Journal of Pediatrics 2021年第2期17卷 197-204页

作者： Chang-Fa Sun Han Liu Yan-Hui Hao Hong-Tao Hu Zhi-Yang Zhou Ke-Xin Zou Xin-Mei Liu jian-zhong sheng Guo-Lian Ding He-Feng Huang The International Peace Maternity and Child Health Hospital School of MedicineShanghai Jiao Tong University910 Hengshan RoadShanghai 200030China Shanghai Key Laboratory of Embryo Original Diseases ShanghaiChina Department of Pathology and Pathophysiology School of MedicineZhejiang UniversityHangzhouChina Department of Gynecology and Obstetrics The First Affiliated Hospital of Nanjing Medical UniversityNanjingChina

Background Previous studies investigated the association between gestational anemia and neonatal ***,few studies explored whether the effects of gestational anemia could be eliminated by subsequent correction of anemia in the later stages of *** study aimed to investigate the relationship between anemia in different trimesters and neonatal *** The study was conducted in Shanghai,China,with a sample of 46,578 pregnant women who delivered between January 1,2016 and July 1,2019.A multivariable logistic regression model was adopted to analyse the associations between maternal anemia and neonatal *** The incidence of gestational anemia was 30.2%,including 4.4%in the first trimester,9.6%in the second trimester,and 16.2%in the third *** 24.5%(507/2066)of anemia that occurred in the first trimester and 29.6%(1320/4457)that occurred in the second trimester could be corrected in the later stages of *** occurring in the first trimester was associated with small for gestational age[odds ratio(OR)1.46;95%confidence interval(CI)1.20-1.78]and with fetal distress(OR 1.23;95%CI 1.08-1.40).Anemia corrected in the first trimester also was associated with a higher risk of small for gestational *** Gestational anemia is a public health problem in China impacting neonatal *** in pregnancy could be corrected in only about a quarter of the *** in the first trimester,whether corrected or not,still led to lower birth weight;therefore,the prevention of anemia prior to pregnancy is important.

关键词： Hemoglobin Iron deficiency Low birth weight Preterm birth Small for gestational age

Long interpregnancy interval and adverse perinatal outcomes: a retrospective cohort study

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Science China(Life Sciences) 2020年第6期63卷 898-904页

作者： Jing Lin Han Liu Dan-Dan Wu Hong-Tao Hu Hui-Hui Wang Cheng-Liang Zhou Xin-Mei Liu Xiao-Jun Chen jian-zhong sheng He-Feng Huang International Peace Maternity and Child Health Hospital School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Institute of Embryo-Fetal Original Adult Disease Shanghai Key Laboratory for Reproductive MedicineSchool of MedicineShanghai Jiao Tong UniversityShanghai 200030China Shanghai Key Laboratory of Embryo Original Disease Shanghai 200030China Department of Pathology and Pathophysiology School of MedicineZhejiang UniversityHangzhou 310058China

We conducted a retrospective cohort study of 9,552 women experiencing their second delivery between 2014 and 2016 at the International Peace Maternity and Child Health Hospital to investigate the association between the interpregnancy interval(IPI)and adverse perinatal outcomes. With the 12–23-mon IPI as the reference category, logistic regression analyzes were used to examine associations between different IPIs(<12, 12–23, 24–59, 60–119, and ≥120 mon) and perinatal outcomes(gestational diabetes mellitus, premature membrane rupture, gestational hypertension, preterm birth, low birth weight, and macrosomia).Compared with the 12–23-mon IPI category, women with longer IPIs had a higher risk of adverse perinatal outcomes, and those with an IPI ≥120 mon had the highest risk of gestational diabetes mellitus and premature membrane rupture(adjusted odds ratio(OR) 1.76, 95% confidence interval(CI) 1.32–2.35 and adjusted OR 2.03, 95% CI 1.53–2.67, respectively). These results indicate that a longer IPI is associated with a higher risk of adverse perinatal outcomes and an IPI of ≥120 mon appears to be independently associated with a higher risk of gestational diabetes mellitus and premature membrane rupture.

关键词： interpregnancy interval perinatal outcomes gestational diabetes mellitus premature membrane rupture

Adriamycin induces H2AX phosphorylation in human spermatozoa

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Asian Journal of Andrology 2008年第5期10卷 749-757页

作者： zhong-Xiang Li Ting-Ting Wang Yan-Ting Wu Chen-Ming Xu Min-Yue Dong jian-zhong sheng He-Feng Huang Department of Reproductive Endocrinology Women＇s Hospital Zhejiang University School of Medicine Hangzhou 310006 China Department of Pharmacology and Therapeutics Faculty of Medicine University of Calgary Calgary Alberta T2N 4N1 Canada

Aim： To investigate whether adriamycin induces DNA damage and the formation of γH2AX （the phosphorylated form of histone H2AX） foci in mature spermatozoa. Methods： Human spermatozoa were treated with adriamycin at different concentrations, γH2AX was analyzed by immunofluorescent staining and flow cytometry and doublestrand breaks （DSB） were detected by the comet assay. Results： The neutral comet assay revealed that the treatment with adriamycin at 2 μg/mL for different times （0.5, 2, 8 and 24 h）, or for 8 h at different concentrations （0,4, 2 and 10 μg/mL）, induced significant DSB in spermatozoa. Immunofluorent staining and flow cytometry showed that the expression of γH2AX was increased in a dose-dependent and time-dependant manner after the treatment of adriamycin. Adriamycin also induced the concurrent appearance of DNA maintenance/repair proteins RAD50 and 53BP 1 with γH2AX in spermatozoa. Wortmannin, an inhibitor of the phosphatidylinositol 3-kinase （PI3K） family, abolished the co-appearance of these two proteins with γH2AX. Conclusion： Human mature spermatozoa have the same response to DSB-induced H2AX phosphorylation and subsequent recruitment of DNA maintenance/ repair proteins as somatic cells.

关键词： adriamycin human spermatozoa DNA double strand-breaks γH2AX

Pi3k/Pkc Pathway Mediates the Alteration of AQP7Expression and Location in Oocyte Induced by Cryoprotectants and Hyperosmolar Stimulation

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Pi3k/Pkc Pathway Mediates the Alteration of AQP7Expression a...

眼睑恶性肿瘤切除术后异体巩膜移植用于眼睑重建的临床分析

中国中西医结合学会生殖医学分会首届学术年会暨生殖医学专业委员会成立大会

作者： Ya-Jing Tan jian-zhong sheng He-Feng Huang Department of Reproductive Medicine International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong University Department of Pathology & Pathophysiology School of MedicineZhejiang University

Objective:To investigate molecular mechanisms of cryoprotectants and hyperosmolar stimulating oocytes AQP7 expression and localization ***:Detect the expression of AQP3,7 and 9 in human and mouse *** AQP3,7and 9 protein level after exposured in *** AQP7 protein location after exposured in hyperosmolar *** the signaling pathways of kinase activation and

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现代预防医学 2011年第13期38卷 2635-2636+2639页

作者：钟建胜河南省南阳市眼科医院

[目的]探讨眼睑恶性肿瘤切除术后异体巩膜移植在眼睑重建中的临床疗效。[方法]选择2007年6月～2010年9月期间某院收治的眼睑恶性肿瘤切除术患者58例,随机将患者分为研究组和对照组。两组患者经相关检查和明确诊断均行眼睑恶性肿瘤切除术... 详细信息

[目的]探讨眼睑恶性肿瘤切除术后异体巩膜移植在眼睑重建中的临床疗效。[方法]选择2007年6月～2010年9月期间某院收治的眼睑恶性肿瘤切除术患者58例,随机将患者分为研究组和对照组。两组患者经相关检查和明确诊断均行眼睑恶性肿瘤切除术后,对照组患者行自体唇黏膜移植和邻近皮瓣转移进行眼睑重建,而研究组患者则行异体巩膜移植术进行眼睑重建治疗,并对两组患者的临床疗效等相关指标进行对比分析。[结果]与对照组相比,研究组的治愈率和总有效率均显著提高,术后发生上睑较对侧上睑下垂1～2mm的比率和眼睑有轻度切迹的比率均显著降低,差异均具有统计学意义(P﹤0.05)。[结论]异体巩膜移植对于眼睑恶性肿瘤切除术后患者的眼睑重建具有十分重要的临床治疗价值。

关键词：眼睑恶性肿瘤异体巩膜移植眼睑重建

A Method to Solve the Fokker-Planck Equation With Coordinate-dependent Mass,Friction and Temperature

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Chinese journal of nuclear physics 1994年第3期 251-254页

作者： Gu jian-zhong Ling Yin-sheng (Department of Physics,Suzhou University,Suzhou 215006)

An analytical expression of the propagator is obtained by using Lie *** fission rates at the saddle point in both constant and coordinate-dependentmass,friction and temperature cases are calculated based on the expression of thepropagator and local *** numerical calculation for 240pu shows thatthe fission rates from our method are reasonable.

关键词： Fokker-Planck equation Lie algebra method Propagator Coordinatedependent mass friction and temperature Fission rate

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生态文明制度的冲突与整合

政法论丛 2018年第3期 39-49页

作者：钟健生徐忠麟江西理工大学环境资源法研究中心江西赣州341000

生态文明制度分为正式制度和非正式制度,包含法律规范、软法规范、文化规范和习俗规范等四种基本规范。生态文明的正式制度内部及与非正式制度之间都存在一定冲突,影响了我国当前重点加强建设的生态文明法律规范和官方制定的软法规范的... 详细信息

生态文明制度分为正式制度和非正式制度,包含法律规范、软法规范、文化规范和习俗规范等四种基本规范。生态文明的正式制度内部及与非正式制度之间都存在一定冲突,影响了我国当前重点加强建设的生态文明法律规范和官方制定的软法规范的权威和认同,给生态文明法治化带来了一定危害,因而有必要对各类规范整合。生态文明制度的规范整合,应当坚持"一元多样"原则,从文化规范、习俗规范、软法规范和法律规范四个层面着手。文化规范要在生态文明建设中融合与创新,习俗规范要进行现代价值的梳理与选择,软法规范要规范化和系统化,法律规范要通过"自上而下"和"自下而上"的双向互动与其他规范整合。

关键词：生态文明制度法律规范软法规范习俗规范文化规范

Basonuclin 1 deficiency causes testicular premature aging: BNC1 cooperates with TAF7L to regulate spermatogenesis

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Journal of Molecular Cell Biology 2020年第1期12卷 71-83页

作者： Jing-Yi Li Yi-Feng Liu Hai-Yan Xu Jun-Yu Zhang Ping-Ping Lv Miao-E Liu Yan-Yun Ying Ye-Qing Qian Kun Li Cheng Li Yun Huang Gu-Feng Xu Guo-Lian Ding Yu-Chan Mao Chen-Ming Xu Xin-Mei Liu jian-zhong sheng Dan Zhang He-Feng Huang Key Laboratory of Reproductive Genetics(Ministry of Education)and Department of Reproductive Endocrinology Women's HospitalZhejiang University School of MedicineHangzhou 310006China International Peace Maternal and Child Health Hospital Shanghai Jiao Tong UniversityShanghai 200030China Department of Pathology&Pathophysiology Zhejiang University School of MedicineHangzhou 310000China

Basonuclin(BNC1)is expressed primarily in proliferative keratinocytes and gametogenic ***,its roles in spermatogenesis and testicular aging were not *** we discovered a heterozygous BNC1 truncation mutation in a premature ovarian insufficiency *** this study,we found that male mice carrying the truncation mutation exhibited progressively fertility loss and testicular premature ***-wide expression profiling and direct binding studies(by chromatin immunoprecipitation sequencing)with BNC1 in mouse testis identified several spermatogenesis-specific gene promoters targeted by BNC1 including kelch-like family member 10(Klhl1O),testis expressed 14(Tex14)9 and spermatogenesis and centriole associated 1(Spatcl).Moreover,biochemical analysis showed that BNC1 was associated with TATA-box binding protein-associated factor 7 like(TAF7L),a germ cell-specific paralogue of the transcription factor IID subunit TAF7,both in vitro and in testis,suggesting that BNC1 might directly cooperate with TAF7L to regulate *** truncation mutation disabled nuclear translocation of the BNC1/TAF7L complex,thus,disturbing expression of related genes and leading to testicular premature ***,expressions of Y-box-binding protein 2(YBX2),outer dense fiber of sperm tails 1(ODfl),and glyceraldehyde-3-phosphate dehydrogenase,spermatogenic(GAPDHS)were significantly decreased in the testis of men with non-obstructive *** present study adds to the understanding of the physiology of male reproductive aging and the mechanism of spermatogenic failure in infertile men.

关键词： testicular aging spermatogenesis BNC1 TAF7L gene mutation