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Fluorescence Nanoscopy in Neuroscience

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Engineering 2022年第9期16卷 29-38页

作者： Yangyundou Wang jian Lin Qiming Zhang Xi Chen Haitao Luan Min gu Center for Artificial-Intelligence Nanophotonics School of Optical-Electrical and Computer EngineeringUniversity of Shanghai for Science and TechnologyShanghai 200093China

Fluorescence nanoscopy provides imaging techniques that overcome the diffraction-limited resolution barrier in light microscopy,thereby opening up a new area of research in biomedical imaging in fields such as ***,we review the foremost fluorescence nanoscopy techniques,including descriptions of their applications in elucidating protein architectures and mobility,the real-time determination of synaptic parameters involved in neural processes,three-dimensional imaging,and the tracking of nanoscale neural *** conclude by discussing the prospects of fluorescence nanoscopy,with a particular focus on its deployment in combination with related techniques(e.g.,machine learning)in neuroscience.

关键词： Fluorescence imaging Diffraction limit Nanoscale resolution Neuroscience

Tensor Effect on Bubble Nuclei

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Chinese Physics Letters 2011年第10期28卷 62-65页

作者： WANG Yan-Zhao gu jian-Zhong ZHANG Xi-Zhen DONG jian-Min China Institute of Atomic Energy P.O.Box 275(10)Beijing 102413 Center of Theoretical Nuclear Physics National Laboratory of Heavy Ion Accelerator of LanzhouLanzhou 730000 School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000

In the framework of the Hartree–Fock–Bogoliubov(HFB)approach with Skyrme interactions SLy5+T,SLy5+Tw and several sets of TIJ parametrizations,*** Skyrme interaction parametrizations including the tensor terms,the proton density distribution in ^(34)Si and ^(46)Ar nuclei is calculated with and without the tensor *** is shown that the bubble effect in ^(34)Si does not depend a great deal on the Skyrme parametrization and the proton density distribution in ^(34)Si is hardly influenced by the tensor *** to ^(46)Ar,the SLy5+Tw parametrization favors the formation of the bubble structure due to the inversion between the 2s_(1/2) and 1d_(3/2) orbits(2s_(1/2)–1d_(3/2) inversion).The inversion mechanism induced by the SLy5+Tw interaction is analyzed based on the proton single−particle spectra obtained from the SLy5 and SLy5+Tw interactions as well as the wave functions of the 2s_(1/2) and 1d_(3/2) states.

关键词： force tensor bubble

An algorithm for voice conversion with limited corpus

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Chinese Journal of Acoustics 2018年第3期37卷 371-384页

作者： gu Dong jian Zhihua School of Communication Engineering Hangzhou Dianzi University

Under the condition of limited target speaker＇s corpus, this paper proposed an algorithm for voice conversion using unified tensor dictionary with limited corpus. Firstly, parallel speech of N speakers was selected randomly from the speech corpus to build the base of tensor dictionary. And then, after the operation of multi-series dynamic time warping for those chosen speech, N two-dimension basic dictionaries can be generated which constituted the unified tensor dictionary. During the conversion stage, the two dictionaries of source and target speaker were established by linear combination of the N basic dictionaries using the two speakers＇ speech. The experimental results showed that when the number of the basic speaker was 14, our algorithm can obtain the compared performance of the traditional NMF- based method with few target speaker corpus, which greatly facilitate the application of voice conversion system.

关键词： DTW An algorithm for voice conversion with limited corpus

Probabilistic Teleportation of an Arbitrary Two-particle State

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Chinese Physics Letters 2001年第12期18卷 1543-1545页

作者： gu Yong-jian ZHENG Yi-Zhuang guO guang-Can Laboratory of Quantum Communication and Quantum Computation University of Science and Technology of ChinaHefei 230026 Department of Physics Weifang UniversityWeifang 261043 Department of Physics Whenzhou Teachers CollegeWenzhou 325027

A scheme for the teleportation of an arbitrary two-particle state via two non-maximally entangled particle pairsis proposed. We show that teleportation can be successfully realized with a certain probability if the receiveradopts an appropriate unitary-reduction strategy. A specific strategy is provided in detail The probability of successful teleportation is determined by the smaller coefficients of the two entangled pairs.

关键词： particle unitary arbitrary

BTB-BACK-TAZ domain protein MdBT2-mediated MdMYB73 ubiquitination negatively regulates malate accumulation and vacuolar acidification in apple

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Horticulture Research 2020年第1期7卷 827-838页

作者： Quan-Yan Zhang Kai-Di gu Jia-Hui Wang jian-Qiang Yu Xiao-Fei Wang Shuai Zhang Chun-Xiang you Da-Gang Hu Yu-Jin Hao National Key Laboratory of Crop Biology Shandong Collaborative Innovation Center of Fruit&Vegetable Quality and Efficient ProductionCollege of Horticulture Science and EngineeringShandong Agricultural UniversityTai-AnShandong 271018China College of Chemistry and Material Science Shandong Agricultural UniversityTai-AnShandong 271018China

As an important primary metabolite,malate plays a key role in regulating osmotic pressure,pH homeostasis,stress tolerance,and fruit quality of *** R2R3-MYB transcription factor(TF)MdMYB73 was identified as a protein that plays a critical role in determining malate accumulation and vacuolar acidification by directly regulating the transcription of aluminum-activated malate transporter 9(MdALMT9),vacuolar ATPase subunit A(MdVHA-A),and vacuolar pyrophosphatase 1(MdVHP1)in *** addition,the bHLH TF MdCIbHLH1 interacts with MdMYB73 and enhances the transcriptional activity of *** previous studies demonstrated that the BTB-BACK-TAZ domain protein MdBT2 can degrade MdCIbHLH1 to influence malate accumulation and vacuolar ***,the potential upstream regulators of MdMYB73 are currently *** this study,we found that MdBT2 directly interacts with and degrades MdMYB73 through the ubiquitin/26S proteasome pathway to regulate malate accumulation and vacuolar acidification.A series of functional assays with apple calli and fruit showed that MdBT2 controls malate accumulation and vacuolar acidification in an MdMYB73-dependent ***,our findings shed light on the mechanism by which the BTB-BACK-TAZ domain protein MdBT2 regulates malate accumulation and vacuolar acidification by targeting MdMYB73 and MdCIbHLH1 for ubiquitination in *** information may help guide traditional breeding programs and fruit tree molecular breeding,and lead to improvements in fruit quality and stress tolerance.

关键词： malate ubiquitin homeostasis

Finite element prediction on the response of non-uniformly arranged pile groups considering progressive failure of pile-soil system

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Frontiers of Structural and Civil Engineering 2020年第4期14卷 961-982页

作者： Qian-Qing ZHANG Shan-Wei LIU Ruo-Feng FENG jian-gu QIAN Chun-Yu CUI Geotechnical and Structural Engineering Research Center Shandong UniversityJinan 250061China Key Laboratory of Geotechrical and Underground Engineering of the Minstry of Education Tongji UniversityShanghai 200092China

A uniform arrangement of individual piles is commonly adopted in the conventional pile group foundation,and basin-shaped settlement is often observed in *** differential settlement of pile groups will decrease the use-safety requirements of building,even cause the whole-building tilt or *** reduce differential settlement among individual piles,non-uniformly arranged pile groups can be *** paper presents a finite element analysis on the response of pile groups with different layouts of individual piles in pile *** the userdefined subroutine FRIC as the secondary development platform,a softening model of skin friction and a hyperbolic model of end resistance are introduced into the contact pair calculation of ABAQUS *** to the response analysis of a single pile,the reliability of the proposed secondary development method of ABAQUS software is verified using an iterative computer *** reinforcing effects of individual piles is then analyzed using the present finite element ***,the response of non-uniformly arranged pile groups,e.g.,individual piles with variable length and individual piles with variable diameter,is analyzed using the proposed numerical analysis *** suggestions on the layout of individual piles are proposed to reduce differential settlement and make full use of the bearing capacity of individual piles in pile groups for practical purposes.

关键词： numerical simulation non-uniformly arranged pile groups differential settlement pile-soil interaction

Evaluation of terrestrial radiation in the Chengdu Plain using 1/250,000-scale geochemical prospecting data

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Nuclear Science and Techniques 2017年第10期28卷 31-36页

作者： Qing-Xian Zhang jian-Kun Zhao Yi gu Liang-Quan Ge Yuan-Qing Huang Sichuan-Provincial Key Laboratory of Nuclear Technologyin Geosciences Chengdu University of TechnologyChengdu 610059China Sichuan Nuclear Geology Institute Chengdu 610061China

Based on the geochemical prospecting data, we have calculated the gamma absorbed dose rates in air 1 m above the ground surface in the Chengdu Plain, analyzed the relationship between the geological conditions and the distribution of the natural radionuclides, and preliminarily studied the influence of the geological conditions on the terrestrial radiation level in the Chengdu Plain. The result shows that the terrestrial radiation level in the Chengdu Plain is slightly lower than the average values of China and varies greatly according to the complex geological conditions.

关键词：成都平原地面辐射地球化学找矿评价天然放射性核素地质条件辐射水平普查数据

Prediction of shelled shrimp weight by machine vision

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第8期10卷 589-594页

作者： Peng-min PAN jian-ping LI gu-lai LV Hui YANG Song-ming ZHU jian-zhong LOU Department of Biosystems Engineering School of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310029 China

The weight of shelled shrimp is an important parameter for grading *** weight prediction of shelled shrimp by contour area is not accurate enough because of the ignorance of the shrimp *** this paper,a multivariate prediction model containing area,perimeter,length,and width was established.A new calibration algorithm for extracting length of shelled shrimp was proposed,which contains binary image thinning,branch recognition and elimination,and length reconstruction,while its width was calculated during the process of length *** model was further validated with another set of images from 30 shelled *** a comparison purpose,artificial neural network(ANN) was used for the shrimp weight *** ANN model resulted in a better prediction accuracy(with the average relative error at 2.67%),but took a tenfold increase in calculation time compared with the weight-area-perimeter(WAP) model(with the average relative error at 3.02%).We thus conclude that the WAP model is a better method for the prediction of the weight of shelled red shrimp.

关键词： Shelled shrimp Image Feature Length extracting Weight prediction Weight-area-perimeter (WAP) model

Effect of cross-section shape of rotating chute on particle movement and distribution at the throat of a bell-less top blast furnace

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Particuology 2019年第3期17卷 194-206页

作者： Mingyin Kou jian Xu Shengli Wu Heng Zhou Kai gu Shun Yao Bingjie Wen School of Metallurgical and Ecological Engineering University of Science and Technology Beijing Beijing 100083 China Department of Metallurgical Engineering College of Materials Science and Engineering Chongqing University Chongqing 400044 China

Rotating chutes are widely used to control the burden distribution and enhance the gas distribution in a blast *** chute cross-section shape has a significant effect on the particle movement and ***,this study developed a three-dimensional model of a blast furnace top with rotating chute using the discrete element *** effect of different chute shapes on the particle movement and radial burden distribution was then *** results showed that the burden stream height at the chute discharge agrees well with the burden stream thickness at the burden surface.A semicircular chute had the largest main striking point,where the chute discharge joins the burden surface,and trapezoidal and rectangular chutes had successively smaller *** semicircular chute helped to obtain a uniform radial size *** trapezoidal chute helped form an aggregated burden stream at low rotating speeds,while the rectangular shape chute achieved this at higher rotating speeds and different chute angles.

关键词： Blast furnace Chute shape Burden size segregation Discrete element method