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世界胃肠外科杂志:英文版(电子版) 2023年第8期15卷 1712-1718页

作者： jian-Hui Chen Mei-Fen Zhang wen-Chao Du Yan-An Zhang Emergency Department Longyan First Affiliated Hospital of Fujian Medical UniversityLongyan 364000Fujian ProvinceChina

BACKGROUND There are many risk factors for severe acute pancreatitis(SAP)complicated with acute gastrointestinal injury(AGI),but few reports on the interaction between these risk *** To analyze the risk factors for SAP complicated with AGI and their interactive *** We selected 168 SAP patients admitted to our hospital between December 2019 and June *** were divided into AGI group and non-AGI group according to whether AGI was *** data and laboratory test data were compared between the two *** risk factors for SAP with concomitant AGI were analyzed using multifactorial logistic regression,and an analysis of the interaction of the risk factors was *** The percentage of patients with multiple organ dysfunction syndrome,acute physiological and chronic health scoring system II(APACHE II)score,white blood cell count and creatinine(CRE)level was higher in the AGI group than in the non-AGI *** was a statistically significant difference between the two groups(P15 and CRE>100μmol/L were risk factors for SAP complicating *** interaction index of APACHE II score and CRE level was *** An APACHE II score>15 and CRE level>100μmol/L are independent risk factors for SAP complicated with AGI,and there is a positive interaction between them.

关键词： Severe acute pancreatitis Acute gastrointestinal injury Risk factors Interactions Acute physiological and chronic health scoring system II Creatinine

Biosynthesis of rumbrins and inspiration for discovery of HIV inhibitors

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Acta Pharmaceutica Sinica B 2022年第11期12卷 4193-4203页

作者： Beifen Zhong Jun Wan Changhui Shang Jiajia wen Yujia Wang jian Bai Shan Cen Youcai Hu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China Institute of Medicinal Biotechnology Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeiing 100050China

Investigation on how nature produces natural compounds with chemical and biological diversity at the genetic level offers inspiration for the discovery of new natural products and even their biological *** polyketide rumbrin(1)is a lipid peroxide production and calcium accumulation inhibitor,which contains a chlorinated pyrrole moiety that is a rare chemical feature in fungal natural ***,we identify the biosynthetic gene cluster(BGC)rum of 1 and its isomer 12E-rumbrin(2)from Auxarthron umbrinum DSM3193,and elucidate their biosynthetic pathway based on heterolo-gous expression,chemical complementation,and isotopic *** show that rumbrins are assembled by a highly reducing polyketide synthase(HRPKS)that uniquely incorporates a proline-derived pyrrolyl-CoA starer unit,and followed by methylation and *** precursor directed biosynthesis was able to yield a group of rumbrin ***,inspired by the presence of a human immunodeficiency virus(HIV)-Nef associated gene in the rum cluster,we predicted and pharmacologically demonstrated rumbrins as potent inhibitors of HIV at the nanomolar *** work enriches the recognition of unconventional starter units of fungal PKSs and provides a new strategy for genome mining-guided drug discovery.

关键词： Rumbrins Polyketide Precursor-directed biosynthesis Resistance gene Anti-HIV activity

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西藏胡黄连中1个新的葫芦烷型糖苷化合物

中国中药杂志 2024年第7期49卷 1882-1887页

作者：吉海杰王舒王冬梅郝淑兰马祥雪仝立国路博丞李梅芳周文静史美军王晞星唐旭东山西省中医药研究院(山西省中医院) 山西太原030012 中国医学科学院北京协和医学院药物研究所北京100050 山西中医药大学山西太原030619 中国中医科学院西苑医院脾胃病研究所北京100091

采用柱色谱方法对西藏胡黄连Picrorhiza scrophulariiflora乙醇提取物进行提取分离,并通过HR-MS、1D和2D NMR等技术鉴定结构,同时采用CCK-8法检测细胞毒性。结果分离得到4个葫芦烷型糖苷,分别为2β-D-葡萄糖氧基-3β,16α,20β-三羟基-9... 详细信息

采用柱色谱方法对西藏胡黄连Picrorhiza scrophulariiflora乙醇提取物进行提取分离,并通过HR-MS、1D和2D NMR等技术鉴定结构,同时采用CCK-8法检测细胞毒性。结果分离得到4个葫芦烷型糖苷,分别为2β-D-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5,25-二烯-22-酮(1)、2β-D-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5,24-二烯-22-酮(2)、25-乙酰氧基-2β-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5-烯-22-酮(3)、25-乙酰氧基-2β-葡萄糖氧基-3β,16α,20β-三羟基-9-甲基-19-去甲羊毛甾-5,23-(E)-二烯-22-酮(4),其中化合物1为1个新的葫芦烷型糖苷。对4种肿瘤细胞的半数抑制浓度均大于100μmol·L^(-1),表明这4个化合物无明显细胞毒作用。

关键词：西藏胡黄连葫芦烷型糖苷细胞毒性

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小柴胡汤治疗奶牛子宫内膜炎临床效果研究

中兽医医药杂志 2024年第4期43卷 1-6页

作者：郭杨黄璐文安林杨剑宋旭琴欧德渊贵州大学动物科学学院贵州贵阳550025 四川省江安县农业农村局四川江安644200

探索小柴胡汤治疗奶牛子宫内膜炎的临床效果与作用机理。选取16头子宫内膜炎患牛,随机分为阳性对照组(5头)和治疗组(11头),另选5头临床健康奶牛为阴性对照组。用药前1 d和用药后2 d、4 d、6 d无菌采集各组5头奶牛子宫内容物,同时进行颈... 详细信息

探索小柴胡汤治疗奶牛子宫内膜炎的临床效果与作用机理。选取16头子宫内膜炎患牛,随机分为阳性对照组(5头)和治疗组(11头),另选5头临床健康奶牛为阴性对照组。用药前1 d和用药后2 d、4 d、6 d无菌采集各组5头奶牛子宫内容物,同时进行颈静脉采血。对11头中药治疗的患牛进行观察,统计治愈情况;将子宫内容物倍比稀释后分别涂布血平板,以平板菌落计数法测定细菌数,用血细胞计数板测定白细胞数,并染色分型;采用ELISA方法检测血清中IL-1和TNF-α含量;体外观察小柴胡汤对3种主要病原菌的抑菌效果。结果显示,小柴胡汤对奶牛子宫内膜炎治愈率为72.7%,好转率为27.3%,有效率为100.0%;用药6 d时,治疗组奶牛子宫内容物中细菌数量显著低于阳性对照组(P<0.05),白细胞总数、中性粒细胞比例均极显著低于阳性对照组(P<0.01),淋巴细胞比例极显著高于阳性对照组(P<0.01),单核细胞比例显著高于阳性对照组(P<0.05);治疗组奶牛血清中IL-1和TNF-α水平均显著低于阳性对照组(P<0.05);小柴胡汤对溶血性链球菌的抑菌杀菌效果较强,最低抑菌浓度和最小杀菌浓度分别为113.64 mg/mL和431.82 mg/mL,对大肠杆菌也有一定抑制作用。小柴胡汤可通过抑菌及降低炎性细胞数量和炎性因子水平,从而达到治疗奶牛子宫内膜炎的作用。

关键词：小柴胡汤子宫内膜炎抑菌奶牛治疗

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铁路平车侧方卸载通用平台设计

制造业自动化 2024年第1期46卷 140-144页

作者：唐玉发刘建安李春伟付文楚矫见波 73089部队徐州221004 中国船舶重工集团应急预警与救援装备股份有限公司武汉430223

针对无固定站台的货运站在装卸载大型车辆或设备时通常需借助吊车或大型叉车进行作业保障且作业效率低下等情形,主要以解决铁路平车侧方卸载为研究思路进行通用平台的方案设计,通过载荷计算校核、加工试制原理样机,最后经过静态和动态... 详细信息

针对无固定站台的货运站在装卸载大型车辆或设备时通常需借助吊车或大型叉车进行作业保障且作业效率低下等情形,主要以解决铁路平车侧方卸载为研究思路进行通用平台的方案设计,通过载荷计算校核、加工试制原理样机,最后经过静态和动态试验验证了所开发平台的可靠性和安全性,为无固定站台货运站大型车辆或设备的安全卸载提供一种解决路线和技术方案。

关键词：无固定站台货运站大型车辆侧方卸载平台通用

Guajamers A-I, Rearranged Polycyclic Phloroglucinol Meroterpenoids from Psidium guajava Leaves and Their Antibacterial Activity

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Chinese Journal of Chemistry 2021年第5期39卷 1129-1137页

作者： Ji-Wu Huang Chuang-Jun Li Jing-Zhi Yang Chuan Li Yu Zhang Ke Liu Yue Yu jian-dong jiang dong-Ming Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

Eight new polycyclic phloroglucinol meroterpenoids guajamers A-H(1-8),a methylated benzoylphloroglucinol meroterpenoid guajamer I(9)representing a new skeleton,and two known analogues(10 and 11)were isolated from the leaves of Psidium *** structures of new molecules were elucidated by detailed analysis of spectroscopic data,and those of 1,2,8,and 9 were unambiguously confirmed by single-crystal X-ray diffraction ***,compounds 1-8 were sesquiterpene and monoterpene-based meroterpenoids with rearranged skeletons,while compound 9 was the first case of 3-alkyl-5-formyl-benzoylphloroglucinol-coupled sesquiterpene containing an unusual C-l-spiro-fused 6/6/9/4 polycyclic *** addition,all the isolated compounds were evaluated for their antibacterial activity against three bacterial strains,and most of them(compounds 2-7,10,and 11)showed antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis with MIC values of 8-32 μmol/*** findings suggested that meroterpenoids isolated from Psidium guajava can be considered as potential antibacterial leading compounds for pharmaceutical industry.

关键词： Psidium guajava Meroterpenoids X-ray crystal structure Structural elucidation Antibacterial activity

Replication of clinical hepatitis B virus isolate and its application for selecting antiviral agents for chronic hepatitis B patients

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World Journal of Gastroenterology 2008年第22期14卷 3490-3496页

作者： Yin-Ping Lu Tao Guo Bao-Ju Wang Ji-Hua dong jian-Fang Zhu Zhao Liu Meng-Ji Lu dong-Liang Yang Department of Virology Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China Division of Clinical Immunology Tongji Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China Institute of Virology Duisburg-Essen University Essen 45122 Germany

AIM: To establish a cell model harboring replicative clinical hepatitis B virus (HBV) isolates and evaluate its application in individualized selection of anti-HBV agents for chronic hepatitis B (CHB) patients. METHODS: The full-length HBV genomic DNA from 8 CHB patients was amplified by polymerase chain reaction (PCR). All the patients were treated with lamivudine for at least seven months and finally became resistant to lamivudine. The amplified HBV DNA fragments were inserted into pHY106 vectors by Sap Ⅰ?digestion. The recombinant plasmids containing 1.1 copies of HBV genome were transiently transfected into Huh7 cell line, and the levels of HBsAg, HBeAg and intercellular HBV replicative intermediates were determined by ELISA and Southern blot analysis, respectively, with or without lamivudine and adefovir treatment. The antiviral treatment with adefovir was administered to the patients and analyzed in parallel. RESULTS: A total of 25 independent HBV isolateswere obtained from the sera of 8 patients, each patient had at least two isolates. One isolate from each individual was selected and subcloned into pHY106 vector, including 5 isolates with YVDD mutation and 3 isolates with YIDD mutation. All recombinant plasmids harboring HBV isolates were transfected into Huh7 cells. The results indicated that HBV genome carried in HBV replicons of clinical HBV isolates could effectively replicate and express in Huh7 cells. Adefovir, but not lamivudine, inhibited HBV replication both in vitro and in vivo, and in vitro inhibition was dose-dependent. CONCLUSION: The novel method described herein enables individualized selection of anti-HBV agents in clinic and is useful in future studies of antiviral therapy for CHB.

关键词： Hepatitis B virus Chronic hepatitis B Hepatitis B virus isolate Antiviral agents

Flight behavior of the sycamore lace bug, Corythucha ciliata, in relation to temperature, age, and sex

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Journal of Integrative Agriculture 2019年第10期18卷 2330-2337页

作者： LU Shao-hui WEI Mei-cai YUAN Guo-jun CUI jian-xin GONG dong-feng Laboratory of Insect Systematics and Evolutionary Biology Central South University of Forestry and TechnologyChangsha 410000P.R.China Henan Academy of Forestry Zhengzhou 450000P.R.China Henan Institute of Science and Technology Xinxiang 453000P.R.China

The flight capacity of different ages and sexes of the sycamore lace bug, Corythucha ciliata, was studied at different temperatures using a flight mill system. The results of regression analysis showed a significant effect of temperature on flight distance (P=0.0082). Temperature did not influence flight duration (P=0.212) or flight speed (P=0.175). The mean flight distance (1 024 m) and mean flight duration were the greatest at 25.2°C. The age of C. ciliata had a significant influence on flight distance (P=0.0005), flight duration (P=0.0005) and flight speed (P=0.026). The 12-d-old adult had the greatest flight distance (887 m), duration (3 875 s) and speed (0.22 m s^–1). Flight distances and flight duration of females were significantly longer than that of males. However, the male had significantly greater flight speed than the female. The insect appears to be capable of long distance flights. The understanding of the optimal age and temperature for the flight of this insect through this study provides a foundation for better management of the insect in China.

关键词： Corythucha ciliata flight capacity tethered flight temperature age and sex

HPLC-MS/MS法测定人血浆中奈玛特韦和利托那韦浓度

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中国新药与临床杂志 2024年第1期43卷 57-61页

作者：徐宗良李盛建钱跹段虎周瑾张倩倩董昕赵亮上海大学医学院上海200444 上海市宝山区罗店医院药剂科上海201908

目的建立高效液相色谱质谱联用(HPLC-MS/MS)法测定人血浆奈玛特韦和利托那韦的血药浓度,并应用于治疗药物监测。方法血浆样品经蛋白沉淀后测定,流动相为0.1%甲酸水溶液(A相)、乙腈(B相),采用等度洗脱,流速为0.3 m L·min^(-1),柱温为40... 详细信息

目的建立高效液相色谱质谱联用(HPLC-MS/MS)法测定人血浆奈玛特韦和利托那韦的血药浓度,并应用于治疗药物监测。方法血浆样品经蛋白沉淀后测定,流动相为0.1%甲酸水溶液(A相)、乙腈(B相),采用等度洗脱,流速为0.3 m L·min^(-1),柱温为40℃,使用EC-C18色谱柱,正离子模式下进行多反应监测。结果用于定量分析的离子通道分别为m/z 500→110(奈玛特韦),m/z721.2→296.3(利托那韦),m/z 614.4→421(内标瑞德西韦)。人血浆中奈玛特韦在0.01~10μg·mL^(-1)、利托那韦在0.01~2.0μg·mL^(-1)浓度范围内线性关系良好,提取回收率分别为79.56%~89.91%、82.03%~89.56%,日间及日内的RSD均小于15%,并且稳定性良好。采用该方法检测了34例新型冠状病毒肺炎(COVID-19)患者口服Paxlovid后的血药浓度,血药浓度范围在1.04~18.0μg·mL^(-1)之间。结论本研究建立的HPLC-MS/MS方法检测快速,灵敏度高,准确性好,能应用于COVID-19患者服用Paxlovid后的血药浓度监测。

关键词：色谱法高压液相串联质谱法奈玛特韦利托那韦血药浓度

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面向智慧城市的地质大数据应用模式研究

城市地质 2024年第2期19卷 250-258页

作者：文诗宝许国卢鹏黄梅婷冯健赵勇南宁市勘测设计院集团有限公司广西南宁530022 南宁市浅表地质大数据工程技术研究中心广西南宁530022

构建一种“地质大数据+X”的应用模式,推动智慧城市建设向低碳、集约方向发展。应用结果表明:基于“地质大数据+BIM”应用模式,实现城市地下溶洞的分布位置及规模可视化,辅助重大工程规划决策分析,在施工过程中有效规避地下管线,减少施... 详细信息

构建一种“地质大数据+X”的应用模式,推动智慧城市建设向低碳、集约方向发展。应用结果表明:基于“地质大数据+BIM”应用模式,实现城市地下溶洞的分布位置及规模可视化,辅助重大工程规划决策分析,在施工过程中有效规避地下管线,减少施工对地下管线的破坏;2013—2016年南宁市万象城地铁站最大平均沉降速率为10.89 mm·a^(-1),累积形变量-31.4 mm,通过“地质大数据+InSAR”应用模式探明地面发生沉降的主要原因为地铁基坑开挖导致的地面承载力下降;通过对地质大数据的插值分析,获取了地铁1号线缓冲500 m范围内圆砾层的厚度等值线图,其中清川站及广西大学站区间圆砾层厚度大于10 m,长期的地下水侵蚀及压力渗透作用将会影响地铁隧道的使用寿命,应进一步加强地铁隧道防渗监测。

关键词：地质大数据智慧城市可视化平台地下空间开发

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