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SCALE EFFECTS OF AIR-WATER FLOWS IN STILLING BASIN OF MULTI-HORIZONTAL SUBMERGED JETS

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Journal of Hydrodynamics 2010年第6期22卷 788-795页

作者： CHEN jian-gang ZHANG jian-min xu Wei-lin WANG Yu-rong State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan University Chengdu 610065China

A series of experiments were carried out on multi-horizontal submerged jets with four different model scales of 1：36, 1：57, 1：80, 1：200. In routine tests, scale effects have to be considered, due to complex vortex structure and strong air entrainment in stilling basin. Our focus was laid on measuring and analyzing the time-averaged pressure distribution, water depth and closed-to-bed velocity in the stilling basin of multi-horizontal submerged jets. The experiments results show that the model scale has but a slight effect on the time-averaged hydraulic characteristics in the stilling basin of multi-horizontal submerged jets, which indicates that the results of time-averaged hydraulic characteristics for a normal pressure model are reliable. However, the scale effects of air entrainment, fluctuation pressure and vortex structure call for further investigations in order to make the results of experiments serve as scientific references for practical engineering.

关键词： multi-horizontal submerged jets Xiangjiaba hydropower station scale effects experimental investigation hydraulic characteristics

Optimization of Two-Dimensional Gel Electrophoresis for Kenaf Leaf Proteins

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Agricultural Sciences in China 2011年第12期10卷 1842-1850页

作者： CHEN Tao QI jian-min xu jian-tang CHEN Pin-pin TAO Ai-fen CHEN Fu-cheng CHEN Wei Key Laboratory for Genetics Breeding and Multiple Utilization of Crops Ministry of Education/Fujian Agriculture and Forestry University Fuzhou 350002. P.R.China

To establish a suitable and effective protocol of protein extraction for two-dimensional gel electrophoresis （2-DE） analysis in kenaf leaf tissues, three extraction methods （trichloroacetic acid/acetone, urea/thiourea, and phenol extraction methods） were applied to the extraction of kenaf leaf protein. The results were compared in regard to protein extraction efficiency, sodiumdodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）, and 2-DE gels. Furthermore, the 2-DE system was optimized for four aspects： the pH range of IPG （immobilized pH gradient） stripes, sampling methods, sample volumes, and concentration of polyacrylamide gels. The data presented showed that the phenol extraction method is the best method to perform 2-DE analysis of kenaf leaf protein. The protein extracted from phenol extraction method reached the purity of （26.40±0.859）%, showed （25.67±1.53） protein bands in one dimension SDS-PAGE gels, and （1 374±54.44） protein spots on 2-DE gels. The research also indicates that kenaf leaf protein spots were distributed mainly within the pH range of 4-8. More clear background with a better distribution effect and many protein spots could be obtained on 2-DE gels under the conditions of active rehydration loading, 24 cm IPG strips （linear pH gradient of 4-7）, 1.4 mg samples, and 12% SDS-PAGE gels.

关键词： kenaf protein extraction proteome two-dimensional gel electrophoresis

Pattern of colorectal surgery and long-term survival:10-year experience from a single center

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World Journal of Gastrointestinal Oncology 2024年第11期16卷 4383-4391页

作者： De-Xiang Zhu Miao Chen Dong-Hao xu Guo-Dong He Ping-Ping xu Qi Lin Li Ren jian-min xu Department of Colorectal Surgery Zhongshan Hospital Fudan UniversityShanghai 200032China

BACKGROUND The incidence of colorectal cancer(CRC)has increased in recent decades,and ranks fourth among males and third among females in *** resection remains the most important treatment modality for curative intent in *** studies found that surgeon volumes and specialization appeared to be associated with improved overall survival(OS).Moreover,numerous reports have suggested that specialization and minimally invasive surgery have gained increased popularity in CRC ***,few studies have specifically examined the role and long-term survival of all stage CRC in a real-world *** To evaluate the effect of surgeon specialization on survival changes and minimally invasive surgery utilization in a real world *** A retrospective analysis on the association between surgeon specialization and OS between 2008 and 2013 in Zhongshan Hospital CRC database was *** demographic,clinicopathologic,surgical and follow-up data were obtained from the CRC *** specialty was categorized as colorectal surgeon(CS)and general surgeon(GS).CRC patients who underwent primary surgical resection were *** A total of 5141 CRC patients who underwent primary surgical resection between 2008 and 2013 were evaluated,1748(34.0%)of these by *** percentage of minimally invasive procedures in the CS group showed an increasing *** was no benefit associated with surgeon specialization for stage I,II and IV *** specialization exhibited a significant association with OS solely among stage III patients,with 5-year OS rates of 76%and 67%for the CS and GSgroups,respectively(P<0.01).Further analyses found that surgeon specialization was significantly associated with survival only in stage III rectal patients,and the 5-year OS rate in the CS group and GS group was 80%and 67%,respectively(P<0.01).CONCLUSION Surgeon specialization is associated with improved OS after primary surgery in stage III rectal patien

关键词： Colorectal surgery minimally invasive surgery Primary location Overall survival Tumor stage Follow-up

Ocular manifestations of Alport syndrome

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International Journal of Ophthalmology(English edition) 2010年第2期3卷 149-151页

作者： jian-min xu Shi-Sheng Zhang Qiong Zhang Ying-ming Zhou Cai-Hong Zhu jian Ge and Ling Wang Department of Ophthalmology Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai 200025 China

AIMTo analyze the clinical manifestation of Alport syndrome, especially the ocular features.

关键词： Alport syndrome anterior lenticonus macular flecks

Self-expandable metallic stent placement plus laparoscopy for acute malignant colorectal obstruction

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World Journal of Gastroenterology 2013年第33期19卷 5513-5519页

作者： Jia-min Zhou Li-Qing Yao jian-min xu Mei-Dong xu Ping-Hong Zhou Wei-Feng Chen Qiang Shi Zhong Ren Tao Chen Yun-Shi Zhong Department of Endoscopic Center Zhongshan HospitalFudan University Department of General Surgery Zhongshan HospitalFudan University

AIM:To investigate the clinical advantages of the stent-laparoscopy approach to treat colorectal cancer(CRC)patients with acute colorectal obstruction(ACO).METHODS:From April 2008 to April 2012,surgeryrelated parameters,complications,overall survival(OS),and disease-free survival(DFS)of 74 consecutive patients with left-sided CRC presented with ACO who underwent self-expandable metallic stent(SEMS)placement followed by one-stage open(n=58)or laparoscopic resection(n=16)were evaluated *** stent-laparoscopy group was also compared with a control group of 96 CRC patients who underwent regular laparoscopy without ACO between January 2010 and December 2011 to explore whether SEMS placement influenced the laparoscopic procedure or reduced long-term survival by influencing CRC oncological ***:The characteristics of patients among these groups were *** rate of conversion to open surgery was 12.5%in the stent-laparoscopy *** function recovery and postoperative hospital stay were significantly shorter(3.3±0.9 d vs 4.2±1.5 d and 6.7±1.1 d vs 9.5±6.7 d,P=0.016 and P=0.005),and surgical time was significantly longer(152.1±44.4 min vs 127.4±38.4 min,P=0.045)in the stent-laparoscopy group than in the stent-open ***-related complications and the rate of admission to the intensive care unit were lower in the stent-laparoscopy *** were no significant differences in the interval between stenting and surgery,intraoperative blood loss,OS,and DFS between the two stent *** with those in the stentlaparoscopy group,all surgery-related parameters,complications,OS,and DFS in the control group were ***:The stent-laparoscopy approach is a feasible,rapid,and minimally invasive option for patients with ACO caused by left-sided CRC and can achieve a favorable long-term prognosis.

关键词： Self-expandable metallic stent Colorectal cancer Endoscopy Laparoscopy Efficiency Safety

Anti-EGFR and anti-VEGF agents:Important targeted therapies of colorectal liver metastases

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World Journal of Gastroenterology 2014年第15期20卷 4263-4275页

作者： Qing-Yang Feng Ye Wei Jing-Wen Chen Wen-Ju Chang Le-Chi Ye De-Xiang Zhu jian-min xu Department of General Surgery Zhongshan HospitalFudan University

Colorectal liver metastasis (CLM) is common worldwide. Targeted therapies with monoclonal antibodies have been proven effective in numerous clinical trials, and are now becoming standards for patients with CLM. The development and application of anti-epidermal growth factor receptor (anti-EGFR) and anti-vascular endothelial growth factor (anti-VEGF) antibodies represents significant advances in the treatment of this disease. However, new findings continue to emerge casting doubt on the efficacy of this approach. The Kirsten rat sarcoma viral oncogene (KRAS) has been proven to be a crucial predictor of the success of anti-EGFR treatment in CLM. Whereas a recent study summarized several randomized controlled trials, and showed that patients with the KRAS G13D mutation significantly benefited from the addition of cetuximab in terms of progress-free survival (PFS, 4.0 mo vs 1.9 mo, HR = 0.51, P = 0.004) and overall survival (OS, 7.6 mo vs 5.7 mo, HR = 0.50, P = 0.005). Some other studies also reported that the KRAS G13D mutation might not be absolutely predictive of non-responsiveness to anti-EGFR therapy. At the same time, “new” RAS mutations, including mutations in neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) and exons 3 and 4 of KRAS, have been suggested to be predictors of a poor treatment response. This finding was first reported by the update of the PRIME trial. The update showed that for patients with non-mutated KRAS exon 2 but other RAS mutations, panitumumab-fluorouracil, leucovorin, and oxaliplatin (FOLFOX)4 treatment led to inferior PFS (HR = 1.28, 95%CI: 0.79-2.07) and OS (HR = 1.29, 95%CI: 0.79-2.10), which was consistent with the findings in patients with KRAS mutations in exon 2. Then, the update of the PEAK trial and the FIRE-III trial also supported this finding, which would reduce candidates for anti-EGFR therapy but enhance the efficacy. In first-line targeted combination therapy, the regimens of cetuximab plus FOLFOX was c

关键词： Oncology Colorectal cancer Liver metastases Chemotherapy Targeted therapy

High patatin like phospholipase domain containing 8 expression as a biomarker for poor prognosis of colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 787-797页

作者： Peng-Yang Zhou De-Xiang Zhu Yi-Jiao Chen Qing-Yang Feng Yi-Hao Mao Ao-Bo Zhuang jian-min xu Department of General Surgery Zhongshan Hospital Fudan UniversityShanghai 200032China

BACKGROUND Patatin like phospholipase domain containing 8(PNPLA8)has been shown to play a significant role in various cancer *** studies have focused on its roles as an antioxidant and in lipid ***,the role of PNPLA8 in colorectal cancer(CRC)progression is *** To explore the prognostic effects of PNPLA8 expression in *** A retrospective cohort containing 751 consecutive CRC patients was ***8 expression in tumor samples was evaluated by immunohistochemistry staining and semi-quantitated with immunoreactive *** patients were divided into high and low PNPLA8 expression groups based on the cut-off va-lues,which were calculated by X-tile *** prognostic value of PNPLA8 was identified using univariate and multivariate Cox regression *** over-all survival(OS)rates of CRC patients in the study cohort were compared with Kaplan-Meier analysis and Log-rank *** PNPLA8 expression was significantly associated with distant metastases in our cohort(P=0.048).CRC patients with high PNPLA8 expression indicated poor OS(median OS=35.3,P=0.005).CRC patients with a higher PNPLA8 expression at either stage I and II or stage III and IV had statistically significant shorter *** patients with left-sided colon and rectal cancer,the survival curves of two PN-PLA8-expression groups showed statistically significant *** analysis also confirmed that high PNPLA8 expression was an independent prog-nostic factor for overall survival(hazard ratio HR=1.328,95%CI:1.016-1.734,P=0.038).

关键词： Biomarker Colorectal cancer Expression level Overall survival Patatin like phospholipase domain containing 8 Prognosis

Early activated hepatic stellate cell-derived molecules reverse acute hepatic injury

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World Journal of Gastroenterology 2015年第14期21卷 4184-4194页

作者： Wen-Ju Chang Lu-Jun Song Tuo Yi Kun-Tang Shen Hong-Shan Wang Xiao-Dong Gao min Li jian-min xu Wei-Xin Niu Xin-Yu Qin Department of General Surgery Zhongshan HospitalInstitute of General SurgeryFudan University

AIM: To test whether hepatic stellate cells(HSCs) at different activation stages play different roles in acetaminophen(APAP)-induced acute liver injury(ALI).METHODS: HSCs were isolated from mouse liver and cultured in *** changes of initiation HSCs [HSCs(5d)] and perpetuation HSCs [HSCs(p3)] were observed by immunofluorescence and transmission electron *** protective effects of HSCderived molecules, cell lysates and HSC-conditioned medium(HSC-CM) were tested in vivo by survival and histopathological *** injury was determined by measuring aminotransferase levels in the serum and by histologic examination of tissue sections under a light ***, to determine the molecular mediators of the observed protective effects of initiation HSCs, we examined HSC-CM using a highdensity protein ***: HSCs(5d) and HSCs(p3) had different morphological and phenotypic ***(5d) presented a star-shaped appearance with expressing α-SMA at non-uniform levels between ***, HSCs(p3) evolved into myofibroblast-like cells without lipid droplets and expressed a uniform and higher level of α-***-CM(5d), but not HSC-CM(p3), provided a significant survival benefit and showed a dramatic reduction of hepatocellular necrosis and panlobular leukocyte infiltrates in mice exposed to ***, this protective effect was abrogated at higher cell masses, indicating a therapeutic window of ***, the protein array screenrevealed that HSC-CM(5d) was composed of many chemokines and growth factors that correlated with inflammatory inhibition and therapeutic *** compared with HSC-CM(p3), higher levels of monocyte chemoattractant protein-1, macrophage inflammatory protein-1γ, hepatocyte growth factor, interleukin-10, and matrix metalloproteinase-2, but lower levels of stem cell factor and Fas-Ligand were observed in HSCCM(5d).CONCLUSION: These data indicated that initiation HSCs and perpetuation

关键词： Hepatic stellate cells Acute liver injury Initiati

Robot-assisted one-stage resection of rectal cancer with liver and lung metastases

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World Journal of Gastroenterology 2015年第9期21卷 2848-2853页

作者： jian-min xu Ye Wei Xiao-Ying Wang Hong Fan Wen-Ju Chang Li Ren Wei jiang Jia Fan Xin-Yu Qin Zhongshan Hospital Fudan University

The Da Vinci Surgical System may help to overcome some of the difficulties of laparoscopy for complicated abdominal *** authors of this article present a case of robot-assisted, one-stage radical resection of three tumors, including robotic anterior resection for rectal cancer, segmental hepatectomy for liver metastasis, and wedge-shaped excision for lung metastasis.A 59-year-old man with primary rectal cancer and liver and lung metastases was operated upon with a one-stage radical resection approach using the Da Vinci Surgical *** and anastomosis of rectal cancer were performed extracorporeally afterundocking the *** procedure was successfully completed in 500 *** surgical complications occurred during the intervention and postoperative period, and no conversion to laparotomy or additional trocars were *** the best of our knowledge, this is the first case of simultaneous resection for rectal cancer with liver and lung metastases using the Da Vinci Surgery System to be *** procedure is feasible and safe and its main advantages for patient are avoiding repeated operation, reducing surgical trauma, shortening recovery time, and early implementation of postoperative adjuvant therapy.

关键词： Robotic surgery minimally invasive colorectal surg