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Expression of a novel dipeptidyl peptidase 8(DPP8)transcript variant,DPP8-v3,in human testis

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Asian Journal of Andrology 2005年第3期7卷 245-255,页

作者： Hui Zhu Zuo-min Zhou li Lu min Xu Hui Wang jian-min li Jia-Hao Sha Key Laboratory of Reproductive Medicine Department of Histology and Embryology Nanjing Medical UniversityNanjing 210029 China

Aim： To investigate the role of a novel dipeptidyl peptidase 8 transcript variant （DPP8-v3） gene in testis development and/or spermatogenesis. Methods： A human testis cDNA microarray was hybridized with mRNA of human adult and fetal testes. Differentially expressed clones were sequenced and characterized and their expression was analyzed by real-time reverse transcription polymerase chain reaction （RT-PCR） and Southern-blot analysis. Results： A new transcript variant of the human dipeptidyl peptidase （DPP8）, exhibiting a 5-fold higher expression level in human adult than that in fetal testes, was cloned and was named DPP8 variant 3 （DPP8-v3）. The full-length sequence of DPP8-v3 was 3,030 bp, encoding a protein of 898 amino acids. Conclusion： DPPS-v3 is a novel human DPP8 transcript variant highly expressed in the adult testis. Similar to DPPIV, DPP8-v3 may play a key role in the immunoregulation of testes and accordingly may influence spermatogenesis and male fertility. （Asian J Androl 2005 Sep; 7： 245-255）

关键词： DPP8 dipeptidyl peptidase IV (DPPIV) DPP8-v3 immunoregulation spermatogenesis testis

Challenges in Atomic-Scale Characterization of High-k Dielectrics and Metal Gate Electrodes for Advanced CMOS Gate Stacks

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Journal of Materials Science & Technology 2009年第3期25卷 289-313页

作者： Xinhua Zhu jian-min Zhu Aidong li Zhiguo liu Naiben ming National Laboratory of Solid State Microstructures Department of Physics Nanjing University Nanjing 210093 China

The decreasing feature sizes in complementary metal-oxide semiconductor （CMOS） transistor technology will require the replacement of SiO2 with gate dielectrics that have a high dielectric constant （high-k） because as the SiO2 gate thickness is reduced below 1.4 nm, electron tunnelling effects and high leakage currents occur in SiO2, which present serious obstacles to future device reliability. In recent years significant progress has been made on the screening and selection of high-k gate dielectrics, understanding their physical properties, and their integration into CMOS technology. Now the family of hafnium oxide-based materials has emerged as the leading candidate for high-k gate dielectrics due to their excellent physical properties. It is also realized that the high-k oxides must be implemented in conjunction with metal gate electrodes to get sufficient potential for CMOS continue scaling. In the advanced nanoscale Si-based CMOS devices, the composition and thickness of interfacial layers in the gate stacks determine the critical performance of devices. Therefore, detailed atomic- scale understandings of the microstructures and interfacial structures built in the advanced CMOS gate stacks, are highly required. In this paper, several high-resolution electron, ion, and photon-based techniques currently used to characterize the high-k gate dielectrics and interfaces at atomic-scale, are reviewed. Particularly, we critically review the research progress on the characterization of interface behavior and structural evolution in the high-k gate dielectrics by high-resolution transmission electron microscopy （HRTEM） and the related techniques based on scanning transmission electron microscopy （STEM）, including high-angle annular dark- field （HAADF） imaging （also known as Z-contrast imaging）, electron energy-loss spectroscopy （EELS）, and energy dispersive X-ray spectroscopy （EDS）, due to that HRTEM and STEM have become essential metrology tools for characterizing the d

关键词： High-k gate dielectrics Metal gate electrodes, CMOS gate stack HRTEM STEM

NUMERICAL INVESTIGATION ON THE HYDRODYNAMIC PERFORMANCES OF A NEW SPAR CONCEPT

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Journal of Hydrodynamics 2007年第4期19卷 473-481页

作者： ZHANG Fan YANG jian-min li Run-pei CHEN Gang State Key Laboratory of Ocean Engineering Shanghai Jiaotong University Shanghai 200030 China

Recently, the spar platform concept develops quickly in the offshore oil and gas exploitations, especially in deep and ultra-deep water, owing to its benign motion performance, excellent stability and adaptation to wide range of water depth. Many new spar concepts have been put forward with the purpose of reducing fabrication difficulty and cost, while meeting the requirements of exploitation in the meantime Based on the aims mentioned above, a new spar concept was presented in this article and its hydrodynamics both in operating and survival conditions was studied by means of numerical simulation. Basic model tests were also conducted to calibrate the numerical approach. Following aspects are highlighted：（1） new spar concept, （2） global performance of the spar concept and （3） mooring line analysis.

关键词： spar platform time-domain coupled analysis hydrodynamic performances

COUPliNG EFFECTS FOR CELL-TRUSS SPAR PLATFORM: COMPARISON OF FREQUENCY-AND TIME-DOMAIN ANALYSES WITH MODEL TESTS

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Journal of Hydrodynamics 2008年第4期20卷 424-432页

作者： ZHANG Fan YANG jian-min li Run-pei CHEN Gang State Key Laboratory of Ocean Engineering Shanghai Jiaotong University Shanghai 200030 China

For the floating structures in deepwater, the coupling effects of the mooring lines and risers on the motion responses of the structures become increasingly significant. Viscous damping, inertial mass, current loading and restoring, etc. from these slender structures should be carefully handled to accurately predict the motion responses and line tensions. For the spar platforms, coupling the mooring system and riser with the vessel motion typically results in a reduction in extreme motion responses. This article presents numerical simulations and model tests on a new cell-truss spar platform in the State Key Laboratory of Ocean Engineering in Shanghai Jiaotong University. Results from three calculation methods, including frequency-domain analysis, time-domain semi-coupled and fully-coupled analyses, were compared with the experimental data to find the applicability of different approaches. Proposals for the improvement of numerical calculations and experimental technique were tabled as well.

关键词： cell-truss spar time-domain coupled analysis model test coupling effects

Maisonneuve injury with no fibula fracture:A case report

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World Journal of Clinical Cases 2021年第15期9卷 3733-3740页

作者： Guang-Ping liu Ji-Gang li Xiao Gong jian-min li Department of Orthopedics Qilu HospitalCheeloo College of MedicineShandong UniversityJinan 250012Shandong ProvinceChina Department of Orthopedics Zibo Central HospitalShandong UniversityZibo 255000Shandong ProvinceChina Department of Radiology Zibo Central HospitalShandong UniversityZibo 255000Shandong ProvinceChina

BACKGROUND Ankle syndesmosis injury is difficult to diagnose accurately at the initial *** diagnosis or improper treatment can lead to chronic *** syndesmosis injury with a concomitant rupture of the interosseous membrane(IOM)is more unstable and *** relationship between this type of injury and Maisonneuve injury,in which the syndesmosis is also injured,has not been discussed in the literature *** SUMMARY A 16-year-old patient sustained left medial malleolar fracture,and the associated inferior tibiofibular syndesmotic instability was *** open reduction and internal fixation of the medial malleolar fracture,inferior tibiofibular syndesmosis diastasis with IOM rupture was detected by auxiliary *** surgical intervention was performed to reduce anatomically and fix with two trans-syndesmosis *** weeks later,the screws were *** the 6-mo follow-up,the patient gained full range of motion of the *** Complete syndesmosis injury with IOM rupture should be considered Maisonneuve-type *** reduction and internal fixation could obtain good outcomes.

关键词： Maisonneuve Syndesmosis injury Interosseous membrane Stress test Classification Case report

NUMERICAL SIMULATION OF IRREGULAR WAVE-SIMULATING IRREGULAR WAVE TRAIN

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Journal of Hydrodynamics 2010年第4期22卷 537-545页

作者： liANG Xiu-feng YANG jian-min li Jun XIAO Long-fei li Xin State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Shanghai 200030 China

In real sea states,damage incidents on offshore floating structures are not due to the whole time series of wave elevation characterized as statistical one but due to few extreme waves or wave groups in irregular wave ***,using CFD tools to precisely simulate predetermined irregular wave train will lay sound basis for understanding the local characteristic of the flow field and impact loads on offshore floating structures when damage incidents *** this article,the generation of single extreme wave is investigated in a numerical wave ***,experimental irregular wave train is decomposed into certain number of small-amplitude *** Fourier series expansion is performed to determine the amplitude and initial phase angle of each wave component.A hydrodynamic transfer function is used to calculate the amplitude of wave-maker motion associated with each wave *** superposition is carried out on all of the wave-maker motion components to get the final wave-maker *** the wave-maker motion as input,simulation of the single extreme irregular wave train is modeled *** the method is applied to simulating a much more complicated irregular wave *** again main features of the complicated irregular wave train are reproduced compared with experiment carried out in the new deepwater experimental basin at Shanghai Jiao Tong *** the simulation,dynamic mesh method is enabled to model the piston-type wave-maker,the Volume Of Fluid（VOF） method is employed to capture the free surface and a dissipation zone is introduced to deal with wave reflection.

关键词： numerical wave tank irregular wave train Volume of Fluid (VOF) dynamic mesh

EXPERIMENTAL INVESTIGATION ON WAVE RUN-UP CHARACTERISTICS ALONG COLUMNS AND AIR GAP RESPONSE OF SEMI-SUBMERSIBLE PLATFORM

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Journal of Hydrodynamics 2011年第5期23卷 625-636页

作者： SHAN Tie-bing YANG jian-min li Xin XIAO Long-fei State Key Laboratory of Ocean Engineering Shanghai Jiao Tong University Shanghai 200240 China

This article mainly concentrates on a large-volume drilling semi-submersible platform, aiming to reveal wave run-up characteristics along square columns and give the relationship between air gap distributions and wave parameters. The tests with fixed model were conducted firstly on its encountering a series of monochromatic waves. A wide range of wave slope （H/L ） were selected to investigate the air gap response in detail. As can be seen, larger wave steepness will generally cause smaller air gap in the same wave period, which indicates nonlinear effects of incoming wave can amplify wave elevation. Model tests with mooring condition were also conducted in the same wave conditions. As was expected, the maximum relative wave elevation reduces obviously compared with the fixed one. However, wave shape close to columns show higher harmonic characteristics due to interaction between waves and the columns of semi-submersible platform. Meaningful conclusions from the model tests are drawn in this article, which is helpful in air gap design of floating offshore platform to a certain extent. In addition, the experimental results will provide an important reference for further research on validation and update of theoretical models of air gap.

关键词： wave run-up air gap diffraction-radiation effects wave steepness

Expression of a novel alternative transcript of the novel retinal pigment epithelial cell gene NORPEG in human testes

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Asian Journal of Andrology 2005年第3期7卷 277-288,页

作者： Wa Yuan Ying Zheng Ran Huo li Lu Xiao-Yan Huang Lan-Lan Yin jian-min li Zuo-min Zhou Jia-Hao Sha Key Laboratory of Reproductive Medicine Department of Histology and Embroyology Nanjing Medical University Nanjing 210029 China

Aim： To identify a novel alternative transcript of the novel retinal pigment epithelial cell gene （NORPEG） expressed in the human testis. Methods： A human testis cDNA microarray was established and hybridized with cDNA probes from human fetal testes, adult testes and human spermatozoa. Differentially expressed clones were sequenced and analyzed. One of these clones was a short transcript of NORPEG which we proceeded to analyze by RT-PCR. Results： The novel short alternative transcript of NORPEG was isolated and named sNORPEG. It was 3486 bp in length and contained a 2952-bp open reading frame, encoding a 110.4-kDa protein of 983 amino acids. Amino acid sequence analysis showed that the sNORPEG protein contains six ankyrin repeats and two coiled-coil domains. It shares a high homology with the NORPEG and ankycorbin proteins in both its sequence and motifs. Blasting the human genome database localized sNORPEG to human chromosome 5pl 3.2-13.3. Expression profiles showed that sNORPEG was expressed in human fetal testes, adult testes and spermatozoa. Moreover, sNORPEG was found to be ubiquitously expressed in human tissues. Conclusion： sNORPEG is expressed in different developmental stages of the testis and encodes a protein that may have roles in human testis development and spermatogenesis. （Asian J Androl 2005 Sep; 7： 277-288）

关键词： alternative transcript NORPEG testis development spermatogenesis

Effect of siRNA interference on nerve growth factor in intervertebral disc inflammation rats

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Asian Pacific Journal of Tropical Medicine 2014年第9期7卷 739-743页

作者： ming-Lei Lang Ai-lin Qin jian-min li Peng Fu Department of Orthopedics Qilu Hospital Affiliated to Shandong University Shengli Oilfield Central Hospital Department of Orthopedics Gaomi People's Hospital

Objective:To investigate the inhibition effect of siRNA interference on NGF induced by inflammatory factor IL-6,and JUL—1 so as to provide novel targets for clinical treatment of discogenic low back ***:The intervertebral disc nucleus and annulus fibrosus cells of rats were separated-The cells were co-cultured with different concentrations(10 nmol/L,20nmol/L,50 nmol/L,100 nmol/L)of IL-6 and IL-1β.The NGF-siRNA was leaded into the cocultured cells with its import ability assessed by flow cytometry instrument tests,hefore and after which the NCF mRNA expression was detected by real-time Q-PCR and the NGF content was detected by ***:Flow cytometry instrument test results showed that the NGFsiRNA cell conversion rate was 99.8%.Real-time Q-PCR detection results showed that compared with negative control group,the NGF mRNA expression of co-cultured cells treated by 10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L IL-6 and IL-1βwere respectively raised 3.4,3.7,4.7,3.7 times which were all significantly down-regulated after the import of *** detection results showed that compared with negative control group,the NGF content of cocultured medium treated by 10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L I-L6 and IL-1βwere respectively raised 2.9,3.3,4.5,7.4 times which were all significantly decreased after the import of ***:These molecular biological results suggest that inflammatory factor IL-6 and IL-1βcould stimulate NCF on intervertebral disc cells in vitro culture model and its efficiency is concentration dependent,while siRNA interference can inhibit the stimulation effect of IL-6 and IL-1βon intervertebral disc cell,which provides a new targets for the clinical treatment of discogenic low back pain.

关键词： RNA Small interfering Nerve growth factor Low back pain Intervertebral disk displacement