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Antisense imaging of colon cancer-bearing nude mice with liposome-entrapped 99m-technetium-labeled antisense oligonucleotides of c-mycmRNA

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World Journal of Gastroenterology 2004年第17期10卷 2563-2566页

作者： jian-GuoZheng tian-ZhiTan DepartmentofNuclearMedicine BeijingHospitalBeijing100730China DepartmentofNuclearMedicine WestChinaHospitalSichuanUniversityChengdu610041SichuanProvinceChina

AIM: To investigate the feasibility for antisense imaging of the colon cancer with liposome-entrapped 99 m-technetium labeled antisense oligonucleotides as ***: Fifteen mer single-stranded aminolinked phosphorothioate antisense oligonucleotides of c-myc mRNA were labeled with .^99mTc-pertechnetate, then purified and finally entrapped with liposomes to form the labeling compounds, liposome-entrapped ^99mTc-labeled antisense oligonucleotides. The LS-174-T cells (colon of adenocarcinoma cell line) were incubated with the labeling compounds to test the uptake rates of LS-174-T cells. Later on, a model of 30 tumor bearing nude mice was constructed by inoculating with 5×10^6 of LS-174-T cells at right flank of each nude mouse. About 10 d later, the model were adminstered by intravenous injection of the liposomeentrapped ^99mTc-labeled antisense oligonucleotides. Then some of the tumour bearing nude mice were sacrificed at 0.5, 1, 2, and 4 h after intravenous injection, and proper quantity of liver, spleen, tumor, etc. was obtained. The tissues were counted in a gamma counter, and after correction for decay and background activity, expressed as a percentage of the injected dose. The others whose anterior and posterior whole-body scans were obtained at 1, 1.5, 2, 4,6 and 24 h with a dual-head bodyscan camera equipped with parallel-hole low-energy collimaters. The ratios of radioactive counts in tumor to that in contralateral equivalent region of abdomen were ***: The uptake rates of LS-174-T cells for liposomeentrapped ^99mTc-labeled antisense oligonucleotides increased as time prolonged and reach the peak (17.77±2.41%) at 7 *** biodistributions showed that the rdioactivity in the tumor (13.46±0.20%) of injected dose was the highest at 2 h of intravenous injection of liposome-entrapped ^99mTclabeled antisense oligonucleotides, and then decreased sharply to 4.58±0.45% at 4 h. The tumor was shown clearly in the whole-body scan at 2 h of intravenou

关键词：敏感性成像结肠癌裸鼠脂质体 99m-锝-标志致敏作用寡核苷酸 c-myc mRNA 消化系统肿瘤

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Numerical Study of Pressure Pulsation of Centrifugal Pumps with the Compressible Mode

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Journal of Thermal Science 2019年第1期28卷 106-114页

作者： YANG jun LIU jun LIU Xiaohua XIE tian School of Energy and Power Engineering University of Shanghai for Science and Technology School of Aeronautics and Astronautics Shanghai Jiao Tong University

The compressible effect of water is often neglected in the simulation of hydraulic machinery. However, based on experimental and numerical study, it is found that the compressibility of water could influence the magnitude of the pressure pulsation at some frequency in the pump. Therefore, in order to investigate the influence of water compressibility, compressible model is established by using Tait equation. The internal flow of centrifugal pump under different conditions is calculated by this model. The calculated results are compared with the incompressible results, and it is indicated that the compressibility of water has little effect on the performance parameters. But it affects the amplitude of pressure fluctuations at some discrete frequency, especially at the outlet of impeller and volute tongue where significant jet-wake and rotor/stator interaction appears respectively. Meanwhile, water compressibility makes greater influence on the flow pulsation under off-design condition. Therefore, it is necessary to consider the compressibility of working medium in the numerical simulation of unsteady flow in centrifugal pumps, especially in area with strong unsteady flow and at off-design condition.

关键词： water compressibility Tait equation unsteady flow pressure pulsations centrifugal pump

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Empirical Likelihood Ratio Confidence Interval for Positively Associated Series

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Acta Mathematicae Applicatae Sinica 2007年第2期23卷 245-254页

作者： jun-jian Zhang College of Applied Sciences Beijing University of Technology Beijing 100022 China College of Mathematics Guangxi Normal University Guilin 541004 China

Empirical likelihood is discussed by using the blockwise technique for strongly stationary, positively associated random variables. Our results show that the statistics is asymptotically chi-square distributed and the... 详细信息

关键词： Empirical likelihood positive association blockwise confidence interval

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Effect of scanning speed during PTA remelting treatment on the microstructure and wear resistance of nodular cast iron

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International Journal of Minerals,Metallurgy and Materials 2014年第4期21卷 363-370页

作者： Hua-tang Cao Xuan-pu Dong Qi-wen Huang Zhang Pan jian-jun Li Zi-tian Fan State Key Laboratory of Materials Processing and Die & Mould Technology School of Materials Science and EngineeringHuazhong University of Science and Technology

The surface of nodular cast iron （NCI） with a ferrite substrate was rapidly remelted and solidified by plasma transferred arc （PTA） to induce a chilled structure with high hardness and favorable wear resistance. The effect of scanning speed on the microstructure, micro-hardness distribution, and wear properties of PTA-remelted specimens was systematically investigated. Microstructural characterization in-dicated that the PTA remelting treatment could dissolve most graphite nodules and that the crystallized primary austenite dendrites were transformed into cementite, martensite, an interdendritic network of ledeburite eutectic, and certain residual austenite during rapid solidifica-tion. The dimensions of the remelted zone and its dendrites increase with decreased scanning speed. The microhardness of the remelted zone varied in the range of 650 HV0.2 to 820 HV0.2, which is approximately 2.3-3.1 times higher than the hardness of the substrate. The wear re-sistance of NCI was also significantly improved after the PTA remelting treatment.

关键词： nodular cast iron surface treatment plasma arc remelting scanning speed microhardness wear resistance

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A method of shadow puppet figure modeling and animation

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Frontiers of Information Technology & Electronic Engineering 2015年第5期16卷 367-379页

作者： Xiao-fang HUANG Shou-qian SUN Ke-jun ZHANG tian-ning XU jian-feng WU Bin ZHU College of Computer Science and Technology Zhejiang University School of Art Zhejiang University of Technology College of Information Science and Technology Zhejiang Shuren University

To promote the development of the intangible cultural heritage of the world, shadow play, many studies have focused on shadow puppet modeling and interaction. Most of the shadow puppet figures are still imaginary,spread by ancients, or carved and painted by shadow puppet artists, without consideration of real dimensions or the appearance of human bodies. This study proposes an algorithm to transform 3D human models to 2D puppet figures for shadow puppets, including automatic location of feature points, automatic segmentation of 3D models, automatic extraction of 2D contours, automatic clothes matching, and animation. Experiment proves that more realistic and attractive figures and animations of the shadow puppet can be generated in real time with this algorithm.

关键词： Shadow play Shadow puppet figure 3D human body Data processing 2D modeling

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Determination of ribose and phosphorus contents in Haemophilus influenzae type b capsular polysaccharide by a quantitative NMR method using a single internal standard

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Chinese Journal of Natural Medicines 2022年第8期20卷 633-640页

作者： QIN Chun-jun HU Jing TONG Wei ZHANG Teng-Teng tian Guang-Zong ZoU Xiao-Peng LIU jian-Kai YIN jian Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122China National&Local Joint Engineering Laboratory for Novel Vaccine Development Technology Beijing Minhai Biotechnology Co.Ltd.Beijing 102600China jiangnan University-Beijing Minhai Biotechnology Joint Research Center of Carbohydrate-Based Vaccines Jiangnan UniversityWuxi 214122China

The ribose and phosphorus contents in Haemophilus influenzae type b(Hib)capsular polysaccharide(CPS)are two important chemical indexes for the development and quality control of Hib conjugate vaccine.A quantitative ^(1)H-and ^(31)P-NMR method using a single internal standard was developed for simultaneous determination of ribose and phosphorus contents in Hib ***(HMPA)was successfully utilized as an internal standard in quantitative ^(1)H-NMR method for ribose content *** ribose and phosphorus contents were found to be affected by the concentration of polysaccharide ***,15–20 mg·L^(−1) was the optimal concentration range of Hib CPS in D_(2)O solution for determination of ribose and phosphorus contents by this *** ribose and phosphorus contents obtained by the quantitative NMR were consistent with those obtained by traditional chemical *** conclusion,this quantitative ^(1)H-and ^(31)P-NMR method using a single internal standard shows good specificity,accuracy and precision,providing a valuable approach for the quality control of Hib glycoconjugate vaccines.

关键词： Haemophilus influenzae type b Capsular polysaccharide Quantitative^(1)H-NMR Quantitative^(31)P-NMR Ribose content Phosphorus content

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Optimization Study of Calcium Leaching From Steelmaking Slag

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Journal of Iron and Steel Research International 2012年第4期19卷 34-38,68页

作者： XU An-jun ZHANG Hui-ning YANG Yi CUI jian HE Dong-feng tian Nai-yuan State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing Beijing 100083China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083 China Ningloo Iron and Steel Co Ltd Ningbo 315000 Zhejiang China

In order to study calcium leaching behavior for the steelmaking slag,factors that influence the leaching yield have been *** results show that granularity of the slag,liquid to solid ratio（in short for L/S）,temperature and reaction time have a significant effect on the leaching *** optimal conditions for leaching are determined as follows：1） the granularity at 75 μm,L/S at 100,temperature at 60 ℃;2） the granularity at 75 μm,L/S at 50,temperature at 40 ℃.Finally,the optimal leaching yield under these conditions is about 15%

关键词： steelmaking slag leaching yield calcium content

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Asymptotic inference for AR(1) panel data

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Applied Mathematics(A Journal of Chinese Universities) 2020年第3期35卷 265-280页

作者： SHEN jian-fei PANG tian-xiao School of Mathematical Sciences Zhejiang UniversityHangzhou 310027China

A general asymptotic theory is given for the panel data AR(1) model with time series independent in different cross sections. The theory covers the cases of stationary process, local to unity process, unit root process, mildly integrated, mildly explosive and explosive processes. It is assumed that the cross-sectional dimension and time-series dimension are respectively N and T. The results in this paper illustrate that whichever the process is, with an appropriate regularization, the least squares estimator of the autoregressive coefficient converges in distribution to a normal distribution with rate at least O(N-1/3). Since the variance is the key to characterize the normal distribution, it is important to discuss the variance of the least squares estimator. We will show that when the autoregressive coefficient ρ satisfies |ρ| 1. ρ = 1 is the critical point where the convergence rate changes radically. The transition process is studied by assuming ρ depending on T and going to 1. An interesting phenomenon discovered in this paper is that, in the explosive case, the least squares estimator of the autoregressive coefficient has a standard normal limiting distribution in the panel data case while it may not has a limiting distribution in the univariate time series case.

关键词： AR(1)model Least squares estimator Limiting distribution Non-stationray Panel data

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Single-Cell Transcriptome Analysis in Plants:Advances and Challenges

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Molecular Plant 2021年第1期14卷 115-126页

作者： Rahul Shaw Xin tian jian Xu Department of Plant Systems Physiology Institute for Water and Wetland ResearchRadboud UniversityHeyendaalseweg 1356525 AJ Nijmegenthe Netherlands Department of Biological Sciences and Centre for Bioimaging Sciences National University of SingaporeSingapore 117543Singapore

The rapid and enthusiastic adoption of single-cell RNA sequencing(scRNA-seq)has demonstrated that this technology is far more than just another way to perform transcriptome *** is not an exaggeration to say that the advent of scRNA-seq is revolutionizing the details of whole-transcriptome snapshots from a tissue to a *** this disruptive technology,it is now possible to mine heterogeneity between tissue types and within cells like never *** enables more rapid identification of rare and novel cell types,simultaneous characterization of multiple different cell types and states,more accurate and integrated understanding of their roles in life processes,and ***,we are only at the beginning of unlocking the full potential of scRNA-seq *** is particularly true for plant sciences,where single-cell transcriptome profiling is in its early stage and has many exciting challenges to *** this review,we compare and evaluate recent pioneering studies using the A rabidopsis root model,which has established new paradigms for scRNA-seq studies in *** also explore several new and promising single-cell analysis tools that are available to those wishing to study plant development and physiology at unprecedented resolution and *** addition,we propose some future directions on the use of scRNA-seq technology to tackle some of the critical challenges in plant research and breeding.

关键词： bioinformatics pipelines cell types and states plant development and physiology single-cell RNA sequencing single-cell transcriptome analysis

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Memristor-based multi-channel pulse coupled neural network for image fusion

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The Journal of China Universities of Posts and Telecommunications 2020年第6期27卷 54-72页

作者： Liu jian Wu Chengmao tian Xiaoping School of Electronic Engineering Xi'an University of Posts and TelecommunicationsXi'an 710121China

Image fusion is widely used in computer vision and image *** that the traditional image fusion algorithm has a certain limitation in multi-channel image fusion,a memristor-based multi-channel pulse coupled neural network(M-MPCNN)for image fusion is *** on a dual-channel pulse coupled neural network(D-PCNN),a novel multi-channel pulse coupled neural network(M-PCNN)is firstly constructed in this *** the exponential growth dynamic threshold model is used to improve the pulse generation of pulse coupled neural network,which can not only avoid multiple ignitions effectively,but can also improve operational efficiency and reduce *** the same time,synchronous capture can also enhance image edge,which is more conducive to image ***,the threshold and synaptic characteristics of pulse coupled neural networks(PCNNs)can be well realized by using a memristor-based pulse *** results show that the proposed algorithm can fuse multi-source images more effectively than existing state-of-the-art fusion algorithms.

关键词： multi-channel memristor pulse coupled neural network

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