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Chinese Chemical Letters 1995年第3期6卷 259-260页

作者： jian Sheng GU jian ying tong and mei hua ma(Department of Chemistry,Suzhou University,Suzhou,215006Dennis G.TUCK)(Dept.of Chem.and Biochem,Univ.of Windsor,Windsor,Ont.,Canada N9B 3P4)

Tris(tropolonato) samarium (Ⅲ) has been prepared in high yield, by the electrochemical oxidation of samarium metal anode in acetonitrile solution of 2-hydroxy-2, 4, 6-cycloheptatrienone (trogolone, HL). The unusual divalent samarium (Ⅱ) complex was formed at the first step of electrosynthesis. The final product, Sm(C7H8O2)3, has been characterized by microanalysis and the studies of IR and 1H1 13C NMR spectra.

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Dispersion modeling and health risk assessment of dioxin emissions from a municipal solid waste incinerator in Hangzhou,China

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2012年第1期13卷 69-78页

作者： Xiao-jun ma Xu-guang jianG Yu-qi JIN Hong-mei LIU Xiao-dong LI tong CHEN jian-hua YAN State Key Laboratory of Clean Energy Utilization Institute for Thermal Power E~Tgineering Zhejiang University Hangzhou 310027 China

The emission of dioxins from municipal solid waste incinerators(MSWIs) has become a widespread *** effect of meteorological parameters(wind speed,atmospheric stability and mixing height) on the hourly ground level concentration(GLC) of dioxins was estimated using air dispersion ***,the health risks of dioxin exposure were evaluated for children and adults using the Nouwen *** total environmental exposure via air inhalation and food ingestion was calculated,based on linear fit *** results indicate that potentially severe pollution from dioxins occurs at a wind speed of 1.5 m/s with atmospheric stability class *** addition,local residents in the study area are exposed to severe weather conditions most of the time,and the risk exposures for children are far higher than those for *** total exposure for children far exceeds the tolerable daily intake of dioxin recommended by the World Health Organization(WHO) of 1-4 pg TEQ/(kg·d) under severe weather *** from modeling calculations of health risk assessment were consistent with dioxin levels obtained during actual monitoring of emissions.

关键词： Dioxins Meteorology Air dispersion model Health risk assessment Dioxin exposure

γ-Aminobutyric acid transporter (GAT1) overexpression in mouse affects the testicular morphology

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Cell Research 2000年第1期10卷 59-69页

作者： ma ying hua JIA hua HU XIAO GANG ZHOU ZHEN tong mei jian FEI LI HE GUO(Shanghai Institute of Cell Biology, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China) ShanghaiInstituteofCellBiology ChineseAcademyofScience.

γ-Aminobutyric acid and GABAergic receptors were previously reported to be distributed in reproductive systems besides CNS and predicted to participate in the modulation of testicular function. γ-Aminobutyric acid transporter was implicated to be involved in this process. However, the potential role of γ-aminobutyric transporter in testis has not been explored. In this study, we investigated the existence of mouse γ-aminobutyric acid transporter subtype I (mGAT1) in testis. Wild-type and transgenic mice, which overexpressing mGAT1 in a variety of tissues, especially in testis, were primarily studied to approach the profile of mGAT1 in testis. Mice with overexpressed mGAT1 develop normally but with reduced mass and size of testis as compared with wild-type. Testicular morphology of transgenic mice exhibited overt abnormalities including focal damage of the spermatogenic epithelium accompanied by capillaries proliferation and increased diameter of seminiferous tubules lumen. Reduced number of spermatids was also found in some seminiferous tubules. Our results clearly demonstrate the presence of GAT1 in mouse testis and imply that GAT1 is possibly involved in testicular function.

关键词： γ-aminobutyric acid transporter testicular morphology mouse.

Transgenic mice overexpressingγ-aminobutyric acid transporter subtypeⅠ develop obesity

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Cell Research 2000年第4期10卷 303-310页

作者： ma ying hua JIA hua HU XIAO GANG ZHOU RUO WANG ZENG ZHEN tong mei jian FEI LI HE GUO Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China Shanghai Institute of harmacy.ankara.edu.tr

Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity.

关键词： γ-aminobutyric acid transporter subtype Ⅰ transgenic mouse obesit€

Semen parameters in men recovered from COVID-19

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Asian Journal of Andrology 2021年第5期23卷 479-483页

作者： tong-Hang Guo mei-ying Sang Shun Bai Hui ma Yang-Yang Wan Xiao-hua jiang Yuan-Wei Zhang Bo Xu Hong Chen Xue-ying Zheng Si-Hui Luo Xue-Feng Xie Chen-Jia Gong jian-Ping Weng Qing-hua Shi The First Affiliated Hospital of USTC Hefei National Laboratory for Physical Sciences at MicroscaleThe CAS Key Laboratory of Innate Immunity and Chronic DiseasesSchool of Life SciencesUniversity of Science and Technology of ChinaHefei 230027China The Clinical Research Hospital(Hefei)of Chinese Academy of Science Hefei 230001China

The novel coronavirus disease(COVID-19)pandemic is emerging as a global health threat and shows a higher risk for men than *** far,the studies on andrological consequences of COVID-19 are *** ascertain the consequences of COVID-19 on sperm parameters after recovery,we recruited 41 reproductive-aged male patients who had recovered from COVID-19,and analyzed their semen parameters and serum sex hormones at a median time of 56 days after hospital *** longitudinal analysis,a second sampling was obtained from 22 of the 41 patients after a median time interval of 29 days from first *** with controls who had not suffered from COVID-19,the total sperm count,sperm concentration,and percentages of motile and progressively motile spermatozoa in the patients were significantly lower at first sampling,while sperm vitality and morphology were not *** total sperm count,sperm concentration,and number of motile spermatozoa per ejaculate were significantly increased and the percentage of morphologically abnormal sperm was reduced at the second sampling compared with those at first in the 22 patients *** there were higher prolactin and lower progesterone levels in patients at first sampling than those in controls,no significant alterations were detected for any sex hormones examined over time following COVID-19 recovery in the 22 *** it should be interpreted carefully,these findings indicate an adverse but potentially reversible consequence of COVID-19 on sperm quality.

关键词： COVID-19 semen sex hormones sperm count sperm motility spermatogenesis

Protective efficacy of an H5/H7 trivalent inactivated vaccine(H5-Re13,H5-Re14, and H7-Re4 strains) in chickens, ducks, and geese against newly detected H5N1, H5N6, H5N8, and H7N9 viruses

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Journal of Integrative Agriculture 2022年第7期21卷 2086-2094页

作者： ZENG Xian-ying HE Xin-wen MENG Fei ma Qi WANG Yan BAO Hong-mei LIU Yan-jing DENG Guo-hua SHI jian-zhong LI Yan-bing TIAN Guo-bin CHEN hua-lan State Key Laboratory of Veterinary Biotechnology National Poultry Laboratory Animal Resource CenterHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China

Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.***.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.***.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.***.4.4 b HA gene, an H5 N6 virus bearing the clade 2.***.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.***.4.4 h or 2.***.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.

关键词： avian influenza H5/H7 trivalent vaccine H5-Re13 H5-Re14 H7-Re4 strains protective efficacy chickens ducks and geese

Protective effect of LIF-huM SCs on the retina of diabetic model rats

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International Journal of Ophthalmology(English edition) 2021年第10期14卷 1508-1517页

作者： Shan-Na Chen Zhi-Gang Xu ying-Xue ma Song Chen Guang-Hui He mei Han Xiang Gao Jun-hua Wang Bin Wu jian Wang Clinical College of Ophthalmology Tianjin Medical UniversityTianjin 300070China Tianjin Eye Hospital Tianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceTianjin 300020China Xiamen Kehong Eye Hospital Xiamen 361000Fujian ProvinceChina Department of Ophthalmology Baoan Central HospitalShenzhen 518000China Department of Ophthalmology Tianjin First Central HospitalTianjin 300192China Department of Vitreous and Retinopathy Tianjin Eye HospitalTianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceTianjin 300020China Department of Ophthalmology Tianjin Eye HospitalTianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceTianjin 300020China Studying for a Doctor of Medicine Degree from Nankai University Tianjin 300000China

AIM:To investigate the protective effect of human umbilical cord mesenchymal stem cells(hUCMSCs)modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible ***:A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor(LIF)was *** was verified by fluorescent quantitative polymerase chain reaction(qPCR).Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group(group A),streptozotocin-induced diabetic control group(group B),diabetic rats at 3mo injected with empty vector-transfected hUCMSCs(group C)or injected with LIF-hUCMSCs(group D).Four weeks after the intravitreal injection,analyses in all groups included retinal function using flash electroretinogram(F-ERG),retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran(FITCdextran),and retinal structure examination of sections using hematoxylin and eosin *** levels of adiponectin(APN),high-sensitivity C-reactive protein(hsCRP),and neurotrophin-4(NT-4)in each group was detected using immunohistochemistry,PCR,Western blotting,and ELISA,***:A stable transgenic cell line of LIF-hUCMSCs was constructed.F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A,severe damage of the retinal blood vessels and function in group B,and improved retinal structure and function in group C and especially group ***,ELISA,and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B,C,and D than in group ***-CRP expression was significantly higher in group B than in groups A,C,and D,and was significantly higher in group C than in group D(P<0.05).CONCLUSION:LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.

关键词： leukemia inhibitory factor human umbilical cord mesenchymal stem cells diabetic retinopathy diabetic rats adiponectin high-sensitivity C-reactive protein neurotrophin-4

Overexpression of γ-aminobutyric acid transporter subtype I leads to susceptibility to Kainic acid-induced seizure in transgenic mice

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Cell Research 2001年第1期11卷 61-67页

作者： ma yinghua, JIA hua HU, WEN JUAN ZHAO, jian FEI, YUN YU, XIAO GANG ZHOU, ZHEN tong mei, LI HE GUO (Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China) (Shanghai Inst 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2Shanghai Institute of Physiology Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.

关键词： γ-aminobutyric acid transporter seizure susceptibility kainic acid electroencephalography transgenic mice

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

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Journal of Integrative Agriculture 2020年第9期19卷 2294-2300页

作者： ZENG Xian-ying CHEN Xiao-han ma Shu-jie WU Jiao-jiao BAO Hong-mei PAN Shu-xin LIU Yan-jing DENG Guo-hua SHI jian-zhong CHEN Pu-cheng jianG Yong-ping LI Yan-bing HU Jing-lei LU tong maO Sheng-gang GUO Xing-fu LIU Jing-li TIAN Guo-bin CHEN hua-lan State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China Harbin Weike Biotechnology Development Company Harbin 150069P.R.China

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.***.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.***.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.

关键词： avian influenza H5/H7 trivalent vaccine Re-11 Re-12 and H7-Re2 strains protective efficacy

Diagnostic performance of a multiplexed gastrointestinal PCR panel for identifying diarrheal pathogens in children undergoing hematopoietic stem cell transplant

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World Journal of Pediatrics 2024年第9期20卷 966-975页

作者： Yue Tao Cheng-Juan Luo Bing-hua Zhang Xin-Yan Shen Rui-Ke Zhao Bei-ying ma Nan Shen Chang-ying Luo jian-Min Wang Yi-Jun Xia Li Xie Jing Chen Xi Mo Pediatric Translational Medicine Institute Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong University1678 Dongfang Rd.Shanghai200127China Department of Hematology and Oncology Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong University1678 Dongfang Rd.Shanghai200127China Department of Infectious Diseases Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Medical Affairs BioMérieux(Shanghai)CompanyLimitedShanghaiChina Clinical Research Institute School of MedicineShanghai Jiao Tong UniversityShanghaiChina

Background Diarrhea is a common complication of hematopoietic stem cell transplantation(HSCT)and is associated with substantial morbidity,but its etiology is often *** of diarrhea in this population include infectious causes,chemotherapy-or medication-induced mucosal injury and graft-versus-host disease(GVHD).Distinguishing these potential causes of diarrhea is challenging since diarrheal symptoms are often multifactorial,and the etiologies often overlap in transplant *** objectives of this study were to evaluate whether the Filmarray gastrointestinal(GI)panel would increase diagnostic yield and the degree to which pre-transplantation colonization predicts post-transplantation *** From November 2019 to February 2021,a total of 158 patients undergoing HSCT were prospectively included in the *** specimens were obtained from all HSCT recipients prior to conditioning therapy,28±7 days after transplantation and at any new episode of *** stool samples were tested by the Filmarray GI panel and other clinical microbiological *** The primary cause of post-transplantation diarrhea was infection(57/84,67.86%),followed by medication(38/84,45.24%)and GVHD(21/84,25.00%).Ninety-five of 158 patients were colonized with at least one gastrointestinal pathogen before conditioning therapy,and the incidence of infectious diarrhea was significantly higher in colonized patients(47/95,49.47%)than in non-colonized patients(10/63,15.87%)(Pmarray GI panel testing improved the diagnostic yield by almost twofold in our study(55/92,59.78%vs.30/92,32.61%).Conclusions Our data show that more than half of pediatric patients who were admitted for HSCT were colonized

关键词： Children Diarrhea Hematopoietic stem cell transplantation Multiplexed polymerase chain reaction