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Sodium selenite promotes neurological function recovery after spinal cord injury by inhibiting ferroptosis

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Neural Regeneration Research 2022年第12期17卷 2702-2709页

作者： Yi-Xin Chen Talifu Zuliyaer Bin Liu Shuang guo De-Gang Yang Feng Gao Yan Yu Ming-Liang Yang Liang-Jie Du jian-Jun Li School of Rehabilitation Medicine Capital Medical UniversityBeijingChina China Rehabilitation Science Institute BeijingChina Center of Neural Injury and Repair Beijing Institute for Brain DisordersBeijingChina Department of Spinal and Neural Functional Reconstruction China Rehabilitation Research CenterBeijingChina Beijing Key Laboratory of Neural Injury and Rehabilitation BeijingChina Department of Rehabilitation Medicine Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina Department of Spine Surgery Hunan Provincial People’s Hospital(The First Affiliated Hospital of Hunan Normal University)ChangshaHunan ProvinceChina

Ferroptosis is a recently discovered form of iron-dependent cell death,which occurs during the pathological process of various central nervous system diseases or injuries,including secondary spinal cord *** has been shown to promote neurological function recovery after cerebral hemorrhage by inhibiting ***,whether selenium can promote neurological function recovery after spinal cord injury as well as the underlying mechanism remain poorly *** this study,we injected sodium selenite(3μL,2.5μM)into the injury site of a rat model of T10 vertebral contusion injury 10 minutes after spinal cord injury *** found that sodium selenite treatment greatly decreased iron concentration and levels of the lipid peroxidation products malondialdehyde and ***,sodium selenite increased the protein and mRNA expression of specificity protein 1 and glutathione peroxidase 4,promoted the survival of neurons and oligodendrocytes,inhibited the proliferation of astrocytes,and promoted the recovery of locomotive function of rats with spinal cord *** findings suggest that sodium selenite can improve the locomotive function of rats with spinal cord injury possibly through the inhibition of ferroptosis via the specificity protein 1/glutathione peroxidase 4 pathway.

关键词： ferroptosis glutathione peroxidase 4 glutathione iron lipid peroxidation neural regeneration secondary injury sodium selenite specificity protein 1 spinal cord injury

Construction of a Food-Grade Expression Vector Based on pMG36e by Using an α-Galactosidase Gene as a Selectable Marker

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Journal of Integrative Agriculture 2014年第8期13卷 1802-1808页

作者： GU Xin-xi TAN jian-xin TIAN Hong-tao ZHANG Yu-lan LUO Yun-bo guo Xing-hua College of Food Science and Technology Agricultural University of Hebei College of F ood Science and Nutritional Engineering China Agricultural University Institute of Microbiology Chinese Academy of Sciences

Construction of a food-grade expression vector for application to lactic acid bacteria（LAB） is of importance for dairy fermentation system. An α-galactosidase（aga） gene encoding an enzyme degrading melibiose was amplified by PCR from the plasmid p RAF800 of Lactococcus lactis NZ9000. The aga gene was introduced into pMG36 e to substitute the p rimary antibiotic selectable marker of pMG36 e, resulting in construction of a new food-grade expression vector pMG36-aga. To testify the expression efficiency of exogenous gene in pMG36-aga, a 1.5 kb long α-amylase（amy） gene from Ba cillus li cheniformis was cloned by PCR and introduced into the plasmid pMG36-aga. The resultant plasimd pMG36-aga-amy was transformed into L. lactis ML23 by electroporation. The positive clones were selected with the medium containing melibiose as the sole carbon source. Th e selection efficiency of aga was 8.71×103 CFU with a standard deviation of 9.1×102 CFU ？g-1 DNA of pMG36-aga. Furthermore, the SDS-PAGE analysis showed that the pMG36-aga-amy expressed a 56.4 kDa protein which was the same as the putati ve molecular weight of α-amylase. The starch plate assay also indicated that L. lactis ML23 displayed high activity of α-amylase by expressing of amy gene of pMG36-aga-amy.

关键词： food-grade expression vector Lactococcus lactis α-galactosidase gene amylase gene pMG36e

Adjuvant radiotherapy for gallbladder cancer:A dosimetric comparison of conformal radiotherapy and intensity-modulated radiotherapy

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World Journal of Gastroenterology 2011年第3期17卷 397-402页

作者： Xiao-Nan Sun Qi wang Ben-Xing Gu Yan-Hong Zhu jian-Bin Hu guo-Zhi Shi Shu Zheng Department of Radiation Oncology of SirRun Run Shaw Hospital Sir Run Run Shaw Institute of ClinicalMedicine of Zhejiang University Zhejiang University School ofMedicine Hangzhou 310016 Zhejiang Province China Cancer Institute (Key Laboratory of Cancer Prevention and Intervention Ministry of Education Key Laboratory ofMolecular Biology in Medical Sciences Zhejiang Province China)The Second Affiliated Hospital Zhejiang University School ofMedicine Hangzhou 310009 Zhejiang Province China

AIM: To assess the efficacy and toxicity of conformal radiotherapy (CRT) and compare with intensity-modulated radiotherapy (IMRT) in the treatment of gallbladder cancer. METHODS: Between November 2003 and January 2010, 20 patients with gallbladder cancer were treated with CRT with or without chemotherapy after surgical resection. Preliminary survival data were collected and examined using both Kaplan-Meier and actuarial analysis. Demographic and treatment parameters were collected. All patients were planned to receive 46-56 Gy in 1.8 or 2.0 Gy per fraction. CRT planning was compared with IMRT. RESULTS: The most common reported acute toxicities requiring medication (Radiation Therapy Oncology Group, Radiation Therapy Oncology Group Grade 2) were nausea (10/20 patients) and diarrhea (3/20). There were no treatment-related deaths. Compared with CRT planning, IMRT significantly reduced the volume of right kidney receiving > 20 Gy and the volume of liver receiving > 30 Gy. IMRT has a negligible impact on the volume of left kidney receiving > 20 Gy. The 95% of prescribed dose for a planning tumor volume using either 3D CRT or IMRT planning were 84.0% ± 6.7%, 82.9% ± 6.1%, respectively (P > 0.05). CONCLUSION: IMRT achieves similar excellent target coverage as compared with CRT planning, while reducing the mean liver dose and volume above threshold dose. IMRT offers better sparing of the right kidney compared with CRT planning, with a significantly lower mean dose and volume above threshold dose.

关键词： Gallbladder cancers Adjuvant treatment Surgery Radiation therapy

Alternative technique to save ischemic bowel segment in management of neonatal short bowel syndrome: A case report

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World Journal of Clinical Cases 2019年第20期7卷 3353-3357页

作者： Lei Geng Lei Zhou guo-jian Ding Xiao-Liang Xu Yu-Mei Wu Ji-Jun Liu Ting-Liang Fu Department of Pediatric Surgery Binzhou Medical University HospitalBinzhou 256603Shandong ProvinceChina Department of Hepatobiliary Surgery Binzhou Medical University HospitalBinzhou 256603Shandong ProvinceChina Department of Neonatology Binzhou Medical University HospitalBinzhou 256603Shandong ProvinceChina Department of Anorectal Surgery Binzhou Medical University HospitalBinzhou 256603Shandong ProvinceChina

BACKGROUND Congenital short bowel syndrome(SBS)associated with malrotation,gut volvulus and jejuno-ileal atresia is a very rare *** is a severe challenge for surgeons to preserve residual ischemic bowel segment in the management of short bowel syndrome,especially in *** SUMMARY We report a newborn baby with gut malrotation associated with jejuno-ileal atresia,congenital SBS and jejunal *** and abdominal distention were *** laparotomy,malrotation associated with jejuno-ileal atresia,congenital SBS and jenunal volvulus was *** total length of the small bowel was 63 cm with proximal jejunal bowel segment measuring 38 cm,including 18 cm necrotic segment below the Treitz’s ligament and 20 cm severe ischemic *** distal part of the small bowel was 25 cm in length and only about 0.8 cm in ***’s procedure,necrotic segment resection and end-to-back duodeno-ileal anastomosis were *** residual severe ischemic jejunum was preserved with single proximal stoma and distal end *** months later,to restore the continuity of the isolated gut segment,end-to-end duodeno-jejunal and jejuno-ileal anastomosis was *** entire functional small bowel length increased to 80 *** fluid therapy and parenteral nutrition were discontinued on the 10th day *** months later,her body weight was 9.5 *** Isolation of severe ischemic bowel segment and staged anastomosis to restore the gut continuity for infants with SBS are safe and feasible.

关键词： Ischemic bowel segment Short bowel syndrome Bowel isolation technique Staged salvaging procedure Case report

A Survey of Application of Complementary and Alternative Medicine in Chinese Patients with Parkinson’s Disease: A Pilot Study

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Chinese Journal of Integrative Medicine 2020年第3期26卷 168-173页

作者： PAN Xue-wei ZHANG Xiao-guang CHEN Xia-chan LU Qi HU Ying-si HAN Li-ya ZHENG jian-le JIN Jie ZHENG guo-qing Department of Neurology the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhouZhejiang Province325000China Department of Neurology Wenzhou Central HospitalTheorem Clinical College of Wenzhou Medical UniversityWenzhouZhejiang Provinee325000China Department of Neurology Ruian People's Hospitalthe Third Affiliated Hospital of Wenzhou Medical UniversityWenzhouZhejiang Province325200China

Objective:To investigate the prevale nee and spectrum of complementary and alternative medicine(CAM)application and its related-factors in Parkinson's disease(PD)patients in ***:A structured questionnaire regarding the use of CAMs was administered to PD patients by face-to-face *** and social variables,clinical characteristics and treatments,and the related factors of PD were also ***:Ninety out of 113 patients(79.6%)reported that they had received CAMs treatment,of which 48.6%(55/113)patients had received CAMs treatment for *** top 3 kinds of CAMs for PD were Chinese materia medica,rehabilitation exercise,and acupuncture *** source of information regarding CAMs treatment mainly came from relatives and friends(51.8%,73 cases),followed by physicians(31.9%,45 cases),and medias such as television,newspapers or the internet(16.3%,28 cases).Fifty percent of CAM users reported a good therapeutic efficacy,whereas 39% patients reported that they felt neither better nor worse after the treatment,while 11% patients claimed worsening of *** correspondence analyses survey found the main factors affecting the selectivity of CAMs ranked in the following order:in come,education,occupati on,habitati on,age at on set,Hoeh n and Yahr staging,PD durati on,and gen ***:CAMs,especially Chin ese materia medica,rehabilitation exercise,and acupuncture,are widely used for PD patients in China.

关键词： Parkinson's disease complementary and alternative medicine Chinese medicine

煨脓长肉理论在肛肠疾病术后中的应用及安全性分析

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河北中医药学报 2024年第1期39卷 14-16,21页

作者：王娜许建成戚文月田茂生郭姣姣马茜高记华李利霞河北省中医药科学院石家庄050011 河北省中医院石家庄050011 河北中医药大学石家庄050091

目的:评价煨脓长肉换药法促进肛肠疾病术后创面愈合的临床疗效及安全性。方法:将110例肛肠疾病患者随机分为治疗组和对照组,均手术治疗,术后治疗组紫榆膏纱条换药,对照组凡士林纱条换药。比较2组术后第7、14、21 d疼痛评分、创面感染、... 详细信息

目的:评价煨脓长肉换药法促进肛肠疾病术后创面愈合的临床疗效及安全性。方法:将110例肛肠疾病患者随机分为治疗组和对照组,均手术治疗,术后治疗组紫榆膏纱条换药,对照组凡士林纱条换药。比较2组术后第7、14、21 d疼痛评分、创面感染、创面面积以及愈合时间,并对治疗组手术前后安全指标丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、尿素氮(BUN)、肌酐(Cr)进行分析。结果:2组在术后3个时间节点的疼痛评分、创面感染、创面面积以及愈合时间相比较,存在差异(P0.05)。结论:紫榆膏纱条换药可减轻肛肠疾病术后疼痛,促进创面愈合,减少术后创面感染,安全、无副作用。

关键词：煨脓长肉紫榆膏肛肠疾病创面愈合

Pathological Change of Chronic Hepatitis B Patients with Different Tongue Coatings by Circular Multi-omics Integrated Analysis

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Chinese Journal of Integrative Medicine 2022年第1期28卷 28-35页

作者： TANG Yi-shuang guo jian-chun XU Lin ZHANG Xiao-na SHEN Xiao-ping HAI Ya-mei MAO Yu-feng HU Yi-yang ZHAO Yu Key Laboratory of Liver and Kidney Diseases Institute of Liver DiseasesShuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai(201203)China Department of Integrated Chinese and Western Medicine Hangzhou Xixi HospitalHangzhou(310023)China National Engineering Center for Biochip at Shanghai Shanghai(201203)China Institute of Clinical Pharmacology Shuguang Hospital affiliated to Shanghai University of Traditional Chinese MedicineShanghai(201203)China Shanghai Key Laboratory of Traditional Chinese Clinical Medicine Shanghai(201203)China

Objective:To compare the circular pathological changes of chronic hepatitis B(CHB)patients according to the tongue ***:Totally 41 CHB patients with typical white tongue coating(WTC)or yellow tongue coating(YTC)were enrolled and 14 healthy volunteers with normal tongue manifestation served as *** mRNA expression of peripheral leukocytes was detected by GeneChips,and 9 genes were randomly selected for expression *** metabolites were detected by gas chromatographymass *** information was analyzed based on ingenuity pathways analysis or metabolomics database and the integrated networks were constructed by ***:A total of 945 and 716 differentially expressed genes were found in patients with WTC and YTC relative to healthy volunteers *** biological information analysis indicated that CHB patients had obviously increased functions in cell death,apoptosis and necrosis(Z-score 2,P<0.05)and decreased activation in T lymphocytes(Z-score–2,P<0.05),regardless of the tongue *** to patients with WTC,the YTC patients were predicted to be more active in functions related to virus replication(Z-score 2,P<0.05),and the content of circular fatty acids,such as oleic acid(P=0.098)and lauric acid(P=0.035),and citric acid cycle-related metabolites were higher in the YTC patients(P<0.1).The integrated analysis based on differential genes and metabolites indicated that the most difference in the biological function network between the WTC and YTC patients was tumor necrosis factor receptor associated factor 6 mediated-nuclear factor kappa-B activation ***:CHB patients with YTC had more severe inflammation and fatty acids metabolism aberrant than patients with *** results facilitate the modern pathological annotation of Chinese medicine tongue diagnosis theory and provide a reference for the interpretation of pharmacological mechanisms of Chinese medicine treatment.

关键词： Chinese medicine chronic hepatitis B tongue diagnosis metabolomics transcriptomic

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基于图像域检测的SAR杂波干扰评估

电波科学学报 2007年第3期22卷 430-435页

作者：李江源王建国杨建宇电子科技大学电子工程学院四川成都610054

杂波干扰在时域、频域及多普勒域与雷达回波信号具有相似特征,其特点是在SAR成像后形成局部类似杂波的输出效果。为了定量评估干扰效果,图像域采用双参数恒虚警检测方法,通过比较杂波干扰和高斯白噪声干扰对检测性能的影响,验证了杂波... 详细信息

杂波干扰在时域、频域及多普勒域与雷达回波信号具有相似特征,其特点是在SAR成像后形成局部类似杂波的输出效果。为了定量评估干扰效果,图像域采用双参数恒虚警检测方法,通过比较杂波干扰和高斯白噪声干扰对检测性能的影响,验证了杂波干扰优于白噪声干扰的效果以及基于图像域检测方法对干扰效果评估的有效性。

关键词：合成孔径雷达杂波干扰双参数恒虚警检测

Meta-analysis of randomized trials comparing the effectiveness of on-pump and off-pump coronary artery bypass

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Chinese Medical Journal 2012年第2期125卷 338-344页

作者： Chen Yong-bing Shu jian Yang Wen-tao Shi Li guo Xu-feng wang Fei-ge Qian Yong-yue Soochow Univ Affiliated Hosp 2 Dept Cardiothorac Surg Suzhou 215004 Jiangsu Peoples R China

Background The growing enthusiasm for coronary artery bypass grafting （CABG） without cardiopulmonary bypass （CPB） is emerging,but the role of off-pump coronary artery bypass （OPCAB） in clinical practice remains *** purpose of this study was to assess differences in the incidences of stroke,atrial fibrillation （AF）,and myocardial infarction （MI） between OPCAB and conventional coronary artery bypass grafting （CCABG） by meta-analyses of randomized clinical *** A literature search for the period before March 2010 supplemented with manual bibliographic review was performed for all Chinese or English publications in Medline,the Science Citation Index Expanded,the Cochrane Central Register of Controlled Trials （CENTRAL） and CBMdisc.A systematic overview （meta-analyses） of randomized clinical trials was conducted to evaluate the differences between OPCAB and CCABG in the incidences of stroke,AF,and *** meta-analysis was performed using RevMan 5 *** Forty-three randomized clinical trials were selected for meta-analysis after screening a total of 356 references,with 8104 patients in the OPCAB group and 8724 cases in the CCABG *** meta-analyses of these trials showed no significant difference between OPCAB and CCABG in the incidences of stroke （odds ratio （OR）=0.80,95% confidence interval （CI）=0.52-1.22,P=0.30） and MI （OR=0.73,95%CI=0.52-1.02,P=0.06）.However,we found a significantly reduced risk of AF （OR=0.65,95%CI =0.52-0.82,P=0.0002）in off-pump *** Our meta-analyses suggest that OPCAB reduces the risk of postoperative AF compared with CCABG,but there is no significant difference in the incidences of stroke and MI between OPCAB and CCABG.

关键词： coronary artery bypass grafting off pump stroke atrial fibrillation myocardial infarction randomized clinical trials