检索结果-南通市图书馆

Advance and Application of Single‑cell Transcriptomics in Auditory Research

Neuroscience Bulletin 2024年第7期40卷 963-980页

作者： Xiangyu Ma jiamin guo Mengyao Tian Yaoyang Fu Pei Jiang Yuan Zhang Renjie Chai State Key Laboratory of Digital Medical Engineering Department of Otolaryngology Head and Neck SurgeryZhongda HospitalSchool of Life Sciences and TechnologyAdvanced Institute for Life and HealthJiangsu Province High‑Tech Key Laboratory for Bio‑Medical ResearchSoutheast UniversityNanjing 210096China Department of Psychiatry Affiliated Hangzhou First People’s HospitalZhejiang University school of MedicineHangzhou 310030China Department of Otolaryngology Head and Neck Surgery Affiliated Drum Tower Hospital of Nanjing University Medical SchoolJiangsu Provincial Key Medical Discipline(Laboratory)NanjingChina Research Institute of Otolaryngology Nanjing 210008China Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072China Co‑Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 101408China Beijing Key Laboratory of Neural Regeneration and Repair Capital Medical UniversityBeijing 100069China

Hearing loss and deafness,as a worldwide disability disease,have been troubling human ***,the auditory organ of the inner ear is highly heterogeneous and has a very limited number of cells,which are largely uncharacterized in ***,with the development and utilization of single-cell RNA sequencing(scRNA-seq),researchers have been able to unveil the complex and sophisticated biological mechanisms of various types of cells in the auditory organ at the single-cell level and address the challenges of cellular heterogeneity that are not resolved through by conventional bulk RNA sequencing(bulk RNAseq).Herein,we reviewed the application of scRNA-seq technology in auditory research,with the aim of providing a reference for the development of auditory organs,the pathogenesis of hearing loss,and regenerative *** about spatial transcriptomic scRNA-seq,single-cell based genome,and Live-seq technology will also be discussed.

关键词： Single-cell RNA sequencing Inner ear Cochlear Auditory sensory epithelium Spiral ganglion neuron

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp(Mylopharyngodon piceus)

引用

Aquaculture and Fisheries 2024年第2期9卷 226-233页

作者： Yafan Dai Yubang Shen jiamin guo Hao Yang Feng Chen Wei Zhang Wenhui Wu Xiaoyan Xu Jiale Li Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural AffairsShanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Centre of Aquaculture Shanghai Ocean UniversityShanghai201306China

Both in nature and in aquaculture,fish may experience periods of food scarcity or *** metabolic regulation of fish when nutritional state changes is a complex process that involves many *** study glucose metabolism adaptability during fasting and re-feeding in the black carp(Mylopharyngodon piceus),we measured changes in some biochemical indicators related to glucose *** fish were sampled on days 0,1,3,5,and 10 of fasting(F,S1,S3,S5,and S10,respectively)and days 1,3,and 5 of re-feeding(RF1,RF3,and RF5,respectively).The serum glucose concentration decreased significantly at S1,reached the lowest point at S10,and increased significantly at RF1(P<0.05).The concentration of liver glycogen decreased significantly at S1 and reached the lowest level at S3,whereas the muscle glycogen level decreased significantly at S5 and reached the lowest value at S10(P<0.05).Both liver and muscle glycogen levels returned to the pre-fasting level at RF5(P<0.05).Regarding glycolysis,the concentrations of pyruvate kinase(PK)and hexokinase(HK)decreased significantly at S5 and increased significantly at RF5 and RF1,respectively(P<0.05).The concentrations of glucokinase(GCK)and insulin decreased significantly at S1 and increased significantly at RF1 and RF3,respectively(P<0.05).The mRNA expression levels of liver GCK and glucose transporter 2(GLUT2)decreased significantly at S1 and increased significantly at RF1 and RF5,respectively(P<0.05).As for gluconeogenesis,the concentration of glucose-6-phosphatase(G6PC)increased significantly at S1 and decreased significantly at RF1(P<0.05).The concentrations of glucagon and glucocorticoid(GC)increased significantly at S3 and significantly decreased at RF1 and RF5,respectively(P<0.05).The mRNA expression levels of liver G6PC and phosphoenolpyruvate carboxykinase(PEPCK)increased significantly at S3 and S1,and both decreased significantly at RF1(P<0.05).These results indicate that coordination between glycolysis and gluconeogen

关键词： Mylopharyngodon piceus Fasting Glucose metabolism Glycolysis Gluconeogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于解析法CFD−DEM的烧结矿立式固定床气固接触特性

引用

工程科学学报 2024年第10期46卷 1753-1763页

作者：张宇李承志郭嘉敏张伟武汉科技大学材料学部武汉430081 武汉科技大学省部共建耐火材料与冶金国家重点实验室武汉430081 武汉科技大学钢铁冶金与资源利用省部共建教育部重点实验室武汉430081

烧结矿与冷却气体充分接触是实现烧结矿立式冷却工艺的关键.采用解析计算流体力学−离散元法(CFD−DEM)方法对烧结矿立式固定床的气固接触特性进行了研究,主要模拟了非规则烧结矿在固定床内的堆积过程,并采用浸没边界法和动态网格细化技... 详细信息

烧结矿与冷却气体充分接触是实现烧结矿立式冷却工艺的关键.采用解析计算流体力学−离散元法(CFD−DEM)方法对烧结矿立式固定床的气固接触特性进行了研究,主要模拟了非规则烧结矿在固定床内的堆积过程,并采用浸没边界法和动态网格细化技术获得颗粒表面的流场信息.结果表明,双粒径均匀混合料床的平均空隙率主要取决于小粒径颗粒,大粒径颗粒主要影响料床的大空隙结构及分布.“A型”偏析(中心区域堆积小粒径颗粒,近壁面区域堆积大粒径颗粒)料床压降显著低于均匀混合料床,但颗粒与气体接触不充分;“B型”偏析(偏析方式与“A型”相反)料床兼顾了低压降和气固充分接触的特点.料床实验压降与模拟压降随气体表观流速的变化趋势保持一致,验证了解析CFD−DEM方法的准确性.

关键词：计算流体力学−离散元法耦合计算浸没边界法网格细化烧结矿立式冷却气固接触

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The single-cell transcriptomic landscape of the topological differences in mammalian auditory receptors

引用

Science China Life Sciences 2024年

作者： Xiangyu Ma Xin Chen Yuwei Che Siyao Zhu Xinlin Wang Shan Gao Jiheng Wu Fanliang Kong Cheng Cheng Yunhao Wu jiamin guo Jieyu Qi Renjie Chai State Key Laboratory of Bioelectronics Department of Otolaryngology Head and Neck Surgery Zhongda Hospital School of Life Sciences and Technology Advanced Institute for Life and Health Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research Southeast University School of Engineering Vanderbilt University Department of Otolaryngology-Head and Neck Surgery Affiliated Drum Tower Hospital of Nanjing University Medical School Jiangsu Provincial Key Medical Discipline (Laboratory) Research Institute of Otorhinolaryngology Nanjing Department of Neurology Aerospace Center Hospital School of Life Sciences Beijing Institute of Technology Advanced Technology Research Institute Beijing Institute of Technology Co-innovation Center of Neuroregeneration Nantong University Department of Otolaryngology Head and Neck Surgery Sichuan Provincial People's Hospital University of Electronic Science and Technology of China Southeast University Shenzhen Research Institute

Mammalian hair cells (HCs) are arranged spirally along the cochlear axis and correspond to different frequency ranges. Serving as primary sound detectors, HCs spatially segregate component frequencies into a topographical map. HCs display significant diversity in anatomical and physiological characteristics, yet little is known about the organization of the cochleotopic map of HCs or the molecules involved in this process. Using single-cell RNA sequencing, we determined the distinct molecular profiles of inner hair cells and outer hair cells, and we identified numerous position-dependent genes that were expressed as gradients. Newly identified genes such as Ptn, Rxra, and Nfe2l2 were found to be associated with tonotopy. We employed the SCENIC algorithm to predict the transcription factors that potentially shape these tonotopic gradients. Furthermore, we confirmed that Nfe2l2, a tonotopy-related transcription factor, is critical in mice for sensing low-tomedium sound frequencies in vivo. the analysis of cell-cell communication revealed potential receptor-ligand networks linking inner hair cells to spiral ganglion neurons, including pathways such as BDNF-Ntrk and PTN-Scd4, which likely play essential roles in tonotopic maintenance. Overall, these findings suggest that molecular gradients serve as the organizing principle for maintaining the selection of sound frequencies by HCs.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

气固两相粉末燃料爆轰及其推进应用研究进展

引用

兵工学报 2024年第9期45卷 2951-2972页

作者：杨浅舒续晗倪晓冬熊凯郭佳敏翁春生南京理工大学瞬态物理全国重点实验室江苏南京210094

粉末燃料因其能量密度高、稳定性好等优势,作为爆轰推进动力系统的燃料或添加剂,具有广阔的应用前景。根据气固两相粉末爆轰的燃料种类进行分类,并以指导粉末爆轰推进技术应用为导向,回顾粉末燃料在氧化剂氛围以及气体燃料/氧化剂氛围... 详细信息

粉末燃料因其能量密度高、稳定性好等优势,作为爆轰推进动力系统的燃料或添加剂,具有广阔的应用前景。根据气固两相粉末爆轰的燃料种类进行分类,并以指导粉末爆轰推进技术应用为导向,回顾粉末燃料在氧化剂氛围以及气体燃料/氧化剂氛围中起爆特性和传播特性的国内外研究进展,并对二者进行理论和工程技术的研究归纳,概括影响气固两相爆轰起爆和传播特性的因素以及重要的研究结论。从应用前景、推进性能、难点与挑战等角度对两相爆轰发动机研究进行综述,在总结发动机数值和实验研究现状的基础上,对未来需要开展的研究工作进行展望。

关键词：粉末燃料爆轰发动机非均相爆轰混合爆轰起爆与传播特性

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

不对称三单元十三电平逆变器拓扑及其调制策略

引用

高电压技术 2024年第3期50卷 1205-1213页

作者：胡文华余书琨熊志磊郭嘉敏丁文斌刘智雄华东交通大学电气与自动化工程学院南昌330013

相比于对称型级联H桥多电平逆变器,混合级联H桥多电平逆变器可以使用较少H桥单元得到更高质量的输出电压,但传统混合级联多电平逆变器工作时单元间会出现电流倒灌的现象。针对上述问题,对电压比为1:3结构的Ⅲ型逆变器进行了改进,提出了... 详细信息

相比于对称型级联H桥多电平逆变器,混合级联H桥多电平逆变器可以使用较少H桥单元得到更高质量的输出电压,但传统混合级联多电平逆变器工作时单元间会出现电流倒灌的现象。针对上述问题,对电压比为1:3结构的Ⅲ型逆变器进行了改进,提出了一类电压比为3:2:1结构的不对称三单元十三电平逆变器。并针对该逆变器提出了一种融合阶梯波与脉宽调制波优点的混合调制策略,对该调制策略下所得的部分脉冲进行逻辑运算并分配,解决了传统Ⅲ型逆变器所存在的电流倒灌问题,提高了输出电压的电能质量。该混合调制策略下高中压单元工作在较低频率,低压单元工作在较高频率,以保证逆变器的输出电压质量。最后通过仿真和实验共同验证了所提逆变器拓扑结构以及该混合调制策略的正确性和可行性。

关键词：多电平逆变器拓扑优化混合调制逻辑运算电流倒灌

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Simulation of Offshore Wind Turbine Blade Docking Based on the Stewart Platform

引用

Energy Engineering 2023年第11期120卷 2489-2502页

作者： Yi Zhang jiamin guo Huanghua Peng College of Ocean Science and Engineering Shanghai Maritime UniversityShanghai201306China

The windy environment is the main cause affecting the efficiency of offshore wind turbine *** order to improve the stability and efficiency of single-blade installation of offshore wind turbines under high wind speed conditions,the Stewart platform is used as an auxiliary tool to help dock the wind turbine blade in this *** order to verify the effectiveness of the Stewart platform for blade docking,a blade docking simulation system consisting of the Stewart platform,wind turbine blade,and wind load calculation module was built based on Simulink/*** the same time,the PID algorithm is used to control the Stewart platform so that the blade can effectively track the desired trajectory during the docking process to ensure the successful docking of the *** the simulation of the docking process for blades with a length of 61.5 meters,this paper successfully demonstrates a docking system that might facilitate future docking *** also shows that the Stewart platform can effectively reduce the vibration and the movement range of the blade root and improve the stability and efficiency of blade docking.

关键词： Offshore wind turbine Stewart platform blade docking PID simscape multibody

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Subinhibitory Levels of Fluoroquinolones Result in Enrichment of the Membrane Proteome of Staphylococcus aureus

引用

中国海洋大学学报:英文版 2023年第5期22卷 1439-1445页

作者： XU Xiaoxiao LIN Hong guo jiamin LIU Pei SUN Haixin College of Life Sciences Qingdao UniversityQingdao 266071China Food Safety Laboratory College of Food Science and EngineeringOcean University of ChinaQingdao 266003China Institute of Biomedical Engineering Qingdao UniversityQingdao 266071China

Staphylococcus aureus is a common marine foodborne *** this study,antibiotics ciprofloxacin and enrofloxacin were used to induce drug-resistance in *** differentially expressed proteins(DEPs)were analyzed and compared with those in the bacteria cultured without *** primary proteomic alterations were in the levels of cell membrane components and proteins related to lysine and folic acid biosynthesis,which were all significantly *** minimal inhibitory concentrations(MIC)for both test drugs were elevated to 10μg m L^(-1)following serial *** results indicated that,for both ciprofloxacin and enrofloxacin,drug-resistance were developed even in the subinhibitory levels and the primary response was a major alteration in the cell membrane *** changes were similar to those observed in *** cultured with super-MIC levels of these *** current study provides a theoretical basis for in-depth study of the related changes of marine foodborne pathogens in subinhibitory concentrations that are commonly found in situ.

关键词： subinhibitory environment marine foodborne pathogen proteomics fluoroquinolone

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The crosstalk between ferroptosis and anti-tumor immunity in the tumor microenvironment:molecular mechanisms and therapeutic controversy

引用

Cancer Communications 2023年第10期43卷 1071-1096页

作者： Yichen Zheng Lingqi Sun jiamin guo Ji Ma Division of Abdominal Tumor Multimodality Treatment Cancer CenterWest China HospitalSichuan UniversityChengduSichuanP.R.China Department of Neurology Air Force Hospital of the Western Theater of the Chinese People's Liberation ArmyChengduSichuanP.R.China

The advent of immunotherapy has significantly reshaped the landscape of cancer treatment,greatly enhancing therapeutic outcomes for multiple types of ***,only a small subset of individuals respond to it,underscoring the urgent need for new methods to improve its response ***,a recently discovered form of programmed cell death,has emerged as a promising approach for anti-tumor therapy,with targeting ferroptosis to kill tumors seen as a potentially effective *** studies suggest that inducing ferroptosis can synergistically enhance the effects of immunotherapy,paving the way for a promising combined treatment method in the ***,recent research has raised concerns about the potential negative impacts on anti-tumor immunity as a consequence of inducing ferroptosis,leading to conflicting views within the scientific community about the interplay between ferroptosis and anti-tumor immunity,thereby underscoring the necessity of a comprehensive review of the existing literature on this *** reviews on ferroptosis have touched on related content,many focusing primarily on the promoting role of ferroptosis on anti-tumor immunity while overlooking recent evidence on the inhibitory effects of ferroptosis on *** have concentrated solely on discussing related content either from the perspective of cancer cells and ferroptosis or from immune cells and *** that both cancer cells and immune cells exist in the tumor microenvironment,a one-sided discussion cannot comprehensively summarize this ***,from the perspectives of both tumor cells and tumor-infiltrating immune cells,we systematically summarize the current conflicting views on the interplay between ferroptosis and anti-tumor immunity,intending to provide potential explanations and identify the work needed to establish a translational basis for combined ferroptosis-targeted therapy and immunotherapy in treating tumors.

关键词： anti-tumor immunity ferroptosis ferroptosis-targeted therapy immunogenic cell death immunotherapy tumor microenvironment

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Autonomous live working robot navigation with real-time detection and motion planning system on distribution line

引用

High Voltage 2022年第6期7卷 1204-1216页

作者： Haoning Zhao Chaoqun Wang Rui guo Xuewen Rong jiamin guo Qixun Yang Lecheng Yang Yuliang Zhao Yibin Li School of Control Science and Engineering Shandong UniversityJinanChina State Grid Shandong Electric Power Company JinanChina State Grid Intelligence Technology Co. Ltd.JinanChina

In this study,an autonomous robot navigation system is designed for live working on distribution *** developed system features a real-time detection and motion planning system,incorporating a manipulator capable of grasping power *** order to accurately identify targets,the authors propose an object detection method based on the Larger Scale‘You Only Look Once’Version 4(LS-YOLOv4)algorithm for detecting the insulators and drop *** LS-YOLOv4 extracts features of power components by Convolutional Neural Network(CNN),and then performs feature *** the authors develop a motion planning method based on the Node Control Optimal Rapidly Exploring Random Trees(NC-RRT*),which can drive the robot to realise the autonomous robot motion planning and obstacle *** the grasping function,the authors present a reliable Lightweight-based Convolutional Neural Network(L-CNN)grasping point detection ***,the authors evaluate fully autonomous robotic system in both simulated and real-world *** experimental results demonstrate that the proposed system can effectively identify the target and complete the grasping task in an efficient ***,the proposed motion planning method can take into account both planning efficiency and accuracy to manipulation tasks.

关键词： robot system motion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：