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Cardioprotective effect of Danshensu against myocardial ischemia/reperfusion injury and inhibits apoptosis of H9c2 cardiomyocytes via Akt and ERK1/2 phosphorylation

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Cardioprotective effect of Danshensu against myocardial isch...

中华医学会临床药学分会2014年全国学术会议

作者：殷英 Guan Yue duan jialin Wei Guo Zhu Yanrong Quan Wei Guo Chao Zhou Dan Wang Yanhua Xi Miaomiao Wen Aidong Department of Pharmacy Xijing Hospital Fourth Military Medical University

Aim In the present study,we aimed to confirm the cardioprotection of Danshensu(DSS)from myocardial ischemia/reperfusion(Ml/R)injury,and investigate the mechanisms against Ml/R induced *** The rat model of Ml/R injury was induced by a transient vessel occlusion.H9c2 cardiomyocytes was incubated with vehicle or ischemic buffer during hypoxia,and then reoxygenated with or without DSS.

关键词：

Dissection of mechanism of a Chinese medicinal formula,Danhong injection therapy for myocardial ischemia/reperfusion injury in vivo and in vitro

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Dissection of mechanism of a Chinese medicinal formula,Danho...

中华医学会临床药学分会2014年全国学术会议

作者：关月 Yin Ying Zhu YanRong Guo Chao Wei Guo duan jialin Wang YanHua Zhou Dan Quan Wei Weng Yan Xi MiaoMiao Wen AiDong Department of Pharmacy Xijing Hospital Fourth Military Medical University

Aim Danhong injection,a very popular Chinese medicine injection,is reported to be effective for many cardiovascular *** primary active ingredients of DHI,and their systemic mechanism have not been *** We identified th...

关键词：

Dissection of Mechanisms of Chinese Medicinal Formula Danhong Injection, an Effective Treatment for Myocardial Ischemia/Reperfusion Injury

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Dissection of Mechanisms of Chinese Medicinal Formula Danhon...

第十五届中国科协年会第21分会场：中药与天然药物现代研究学术研讨会

作者： GUAN Yue YIN Ying ZHU Yanrong GUO Chao WEI Guo duan jialin WANG Yanhua ZHOU Dan QUAN Wei WENG Yan XI Miaomiao WEN Aidong Fourth Military Medical University

Traditional Chinese medicine uses a systemic treatment approach, targeting multiple etiological factors simultaneously. Danhong injection (DHI), a very popular Chinese medicine injection composed of water-soluble extracts of Radix Salvia miltiorrhiza (Danshen) and Flos Carthamus tinctorius L. (Honghua), is reported to be effective for many cardiovascular conditions. The primary active ingredients of DHI, and their systemic and interrelated mechanism have not been evaluated in an established myocardial ischemia/reperfusion (MI/R) injury model. We identified the main active constituents in DHI, including hydroxysafflor yellow A (A), salvianolic acid B (B), and Danshensu (C), by HPLC fingerprint analysis and assessed their effect on I/R injury rats and cardiomyocytes. These 3 compounds and DHI all decreased the levels of IL-1, TNF-α, and MDA, and increased those of IL-10 and SOD activity in vivo and in vitro, and had anti-apoptotic effects, as shown by flow cytometric analysis and TUNEL assay. Moreover, these compounds increased phosphorylation of Akt and ERK1/2 in cardiomyocytes. Interestingly, we found A, B, and C, exerted prominent effect compared with two other ingredients on inhibiting inflammation, reducing oxidative stress, alleviating apoptosis through decreasing NF-κB level and increasing Nrf2 expression and lower caspase-3 activity, respectively. Compounds A, B, and C are the main active ingredients of DHI. Compound A exerted a more prominent anti-inflammatory effect than B and C, by decreasing NF-κB levels;compound B had more powerful anti-oxidative capacity than A and C, by increasing Nrf2 expression;and compound C had stronger anti-apoptotic ability than A and B, by lowering caspase-3 activity. Our results elucidate the mechanisms by which DHI protects against MI/R induced injury.

关键词： Danhong injection Myocardial ischemia/reperfusion Akt ERK1/2 Inflammation Oxidation Apoptosis. 1

Cardioprotective effects and mechanism of Radix salviae miltiorrhizae and Lignum dalbergiae odoriferae on rat myocardial ischemia/reperfusion injury

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Cardioprotective effects and mechanism of Radix salviae milt...

中国药学会第十三届青年药学科研成果交流会

作者： Fei Mu jialin duan HaiXu Bian Ying Yin YanRong Zhu Guo Wei Yue Guan YanHua Wang Chao Guo AiDong Wen Yong Yang MiaoMiao Xi Department of Pharmacy Xijing HospitalFourth Military Medical University Department of Pharmacy Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

Objective Radix salviae miltiorrhizae（SM） and Lignum dalbergiae odoriferae（DO） are both traditional Chinese medicinal herbs for the treatment of ischemic heart disease and other cardiovascular diseases,but few of literature is about the systematic ***-Dawley rats were pretreated with SM,aqueous extract of DO（DOA） and volatile oil of DO（DOO）,either as monotherapy or in combination for 7 days,then subjected to 30 min of ischemia followed by 180 min of *** pharmacodynamic evaluation and metabonomics based on gas chromatography/time-of-flight mass spectrometry（GC/TOF-MS） were used to identify the therapeutic effect of these traditional Chinese *** results showed that SM,DOA and DOO ameliorated cardiac function respectively,and this effect was further strengthened by their *** all combinations,SM+DOO showed predominant potential（P＜0.01） to improve electrocardiogram and heart rate,reduce heart weight index and myocardial infarct size,decrease the levels of CK-MB and ***,metabonomics-based findings such as the principal component analysis（PCA） and partial least squares discriminant analysis（PLS-DA） score plot of metabolic state in rat serum correlated well to the preceding results,confirming that SM+DOO exerted synergistic therapeutic efficacies to exhibit better effect on MI/R injury rats compared with other pretreatment ***,the maximum effective point of SM+DOO was 30 min and the minimum effective point was 180 *** addition,SM+DOO group markedly（P＜0.01） reduced the number of TUNEL-positive cells,the levels of TNF-α,IL-6 and MDA,while increased the activity of SOD both in serum and *** results indicated that SM+DOO have combined effects that are highly effective than single pretreatment against MI/R injury *** effect might be achieved partly through anti-apoptosis,anti-oxidant and *** SM+DOO might be an effective and promising medicine for both prophylaxis

关键词： Myocardial ischemia/reperfusion injury Radix salviae miltiorrhizae Lignum dalbergiae odoriferae The maximum and minimum effective points Cardioprotection Metabonomics

Total saponins of Aralia taibaiensis protects myocardial cells against oxidative stress through the Nrf2-dependent antioxidant pathway

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Total saponins of Aralia taibaiensis protects myocardial cel...

2013年中国药学大会暨第十三届中国药师周

作者： duan jialin CUI Jia GUAN Yue YIN Ying WEI Guo WENG Yan ZHU Yanrong GUO Chao WANG Yanhua YAN Jiajia WU Xiaoxiao XI Miaomiao WEN Aidong Department of pharmacy Xijing hospital The Fourth Military Medical University

Background: Patients with type 2 diabetes have increased cardiovascular disease risk compared with those without diabetes. Hyperglycemia can induce reactive oxygen species （ROS） generation, which contributes to development of diabetic cardiomyopathy. Our previous study has demonstrated that total saponins of Aralia taibaiensis （sAT）, a frequently-used antidiabetic in traditional Chinese medicine （TCM）, can scavenge free radicals in vivo and have good antioxidant ability on lipid peroxidation of rat liver microsomes. To investigate whether sAT could protect the heart, while it was in the treatment of diabetes, we designed this study to elucidate cardioprotective effects of sAT. Methods and results: Oxidative stress was induced in H9c2 cells by high glucose （33 mM） and glucose oxidase （15mU） and the protective effects of sAT were evaluated. Treatment of H9c2 cells with G/GO resulted in an increase in cell death, intracellular ROS level and cell oxidative injury, which were markedly reduced by sAT treatment. Further study revealed that sAT induced the nuclear translocation of Nrf2, enhanced the expression of Nrf2-related antioxidant proteins heme oxygenase （HO-1） and NAD （P） H quinone oxidoreductase-1 （NQO-1）. Inhibition of Nrf2 by siRNA reduced sAT-induced upregulation of these antioxidant genes. Western blotting and luciferase assay revealed sAT activated Nrf2 via the PI3K/Akt pathway. Moreover, Nrf2 siRNA markedly reduced the cytoprotective effects of sAT. Conclusions: sAT exerts cytoprotective effects against oxidative stress induced by hyperglycemia. This cardioprotection is mediated, at least in part, through PI3K/Akt/Nrf2 pathways, which further activates Nrf2-regulated antioxidant enzymes. sAT might be a promising candidate for the treatment of diabetic cardiomyopathy.

关键词： Type 2 diabetes Reactive oxygen species Antioxidant response element Total saponins of Aralia taibaiensis Nfr2 AKT

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复合铝热剂配方及其性能研究

爆破器材 2019年第6期48卷 39-42页

作者：王志强黄寅生杨研段佳林南京理工大学化工学院

为了改善铝热剂的点火和燃烧性能,采用均匀设计法对Al-Fe 3O 4、Al-Ba(NO 3)2和Al-MnO 2进行配方优化设计,然后选用SPSS(statistical program for social sciences)软件进行二次回归分析,确定了一种铝热剂配方,并外掺占其质量25%的黏结... 详细信息

为了改善铝热剂的点火和燃烧性能,采用均匀设计法对Al-Fe 3O 4、Al-Ba(NO 3)2和Al-MnO 2进行配方优化设计,然后选用SPSS(statistical program for social sciences)软件进行二次回归分析,确定了一种铝热剂配方,并外掺占其质量25%的黏结剂,获得复合铝热剂。研究其燃烧性能,试验结果表明,该复合铝热剂点燃性能好,燃烧过程分成两个阶段,整个燃烧时间持续约140 s,最高燃烧温度达1893.32 K,能够形成较好的高温纵火效果,30 g复合铝热剂能够可靠熔穿2 mm铝板。

关键词：复合铝热剂均匀设计法回归分析

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污水处理系统中厌氧氨氧化细菌的富集策略

北京工业大学学报 2024年第2期50卷 207-215页

作者：冯婉仪李家麟段晨雪彭永臻北京工业大学城镇污水深度处理与资源化利用技术国家工程实验室北京100124

厌氧氨氧化(Anammox)工艺有利于实现可持续的污水生物处理,自发现以来就引起了人们的极大兴趣,与传统的生物脱氮技术相比,其优势在于节约充氧电耗与外加碳源所需的运行费用,以及较低的剩余污泥产量。然而,厌氧氨氧化细菌(AnAOB)的生长... 详细信息

厌氧氨氧化(Anammox)工艺有利于实现可持续的污水生物处理,自发现以来就引起了人们的极大兴趣,与传统的生物脱氮技术相比,其优势在于节约充氧电耗与外加碳源所需的运行费用,以及较低的剩余污泥产量。然而,厌氧氨氧化细菌(AnAOB)的生长速率极其缓慢,限制了Anammox工艺的大规模应用。综述了以往富集AnAOB的研究,主要介绍了以下3个方面:接种污泥选择、载体与反应器设计以及外加化学物质。该文将有助于研究人员规划和设计一个合适的AnAOB富集策略,有利于拓宽Anammox工艺在污水生物处理中的应用。

关键词：厌氧氨氧化生物脱氮污水处理富集工艺启动

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青柿子提取物抑制消化酶活性与降血糖效应

中国食品学报 2024年第5期24卷 264-279页

作者：刘婧任维维屈佳林李珉梦刘小娟段旭昌西北农林科技大学食品科学与工程学院陕西杨凌712100

为探究青柿子提取物对人唾液α-淀粉酶(HSA)、猪胰腺α-淀粉酶(PPA)、α-葡萄糖苷酶(α-Glu)的抑制作用及餐后血糖变化,以青柿子为原料,采用超声辅助丙酮萃取和大孔树脂纯化法制备青柿子提取物,通过酶活性抑制率分析、酶动力学分析和酶... 详细信息

为探究青柿子提取物对人唾液α-淀粉酶(HSA)、猪胰腺α-淀粉酶(PPA)、α-葡萄糖苷酶(α-Glu)的抑制作用及餐后血糖变化,以青柿子为原料,采用超声辅助丙酮萃取和大孔树脂纯化法制备青柿子提取物,通过酶活性抑制率分析、酶动力学分析和酶荧光猝灭试验研究青柿子提取物体外抑制酶的效果及机制;通过小鼠实验验证青柿子提取物对餐后血糖水平的控制作用。结果表明:青柿子提取物抑制HSA、PPA、α-Glu活性,抑制类型均为混合性抑制,半抑制浓度值(IC50)分别为(16.18±0.17),(13.57±0.30),(3.22±0.03)μg/mL;HSA、PPA、α-Glu酶与青柿子提取物均有1个结合位点,说明青柿子提取物作用于HSA、PPA、α-Glu酶的荧光发色基团,自发形成复合物,引起HSA、PPA、α-Glu酶发色基团周围微环境的改变和酶内源性荧光淬灭,从而抑制酶活性。动物实验表明青柿子提取物可明显降低小鼠餐后血糖水平,提示青柿子提取物通过抑制淀粉酶和葡萄糖苷酶活性来降低餐后血糖水平。

关键词：青柿子提取物 α-淀粉酶 α-葡萄糖苷酶酶活性机制餐后血糖

建构视角下西安乡村现代建筑材料应用问题研究

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城市设计 2024年第2期 104-109页

作者：段德罡韩嘉林西安建筑科技大学建筑学院浙江省建筑设计研究院

随着城镇化建设进程的加速,乡村地区的建设与城市逐渐接轨,现代建筑材料在乡村建设中无序使用,导致乡村风貌混乱成为当下乡村问题的集中诟病,以致地域特色消弭。本研究以当下西安乡村地区若干典型村庄为调查对象,以建构理论为基础,从建... 详细信息

随着城镇化建设进程的加速,乡村地区的建设与城市逐渐接轨,现代建筑材料在乡村建设中无序使用,导致乡村风貌混乱成为当下乡村问题的集中诟病,以致地域特色消弭。本研究以当下西安乡村地区若干典型村庄为调查对象,以建构理论为基础,从建造逻辑、组织形式及文化属性三个方面对当前建筑材料应用所带来的风貌问题进行分析,并提出更新策略,以期实现现代建筑材料应用视角下的乡村风貌优化目标。

关键词：乡村风貌现代建筑材料建造逻辑组织形式文化属性