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Characterization of 3D DNA Assemblies Using Cryogenic Electron Microscopy

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Chemical Research in Chinese Universities 2020年第2期36卷 227-236页

作者： WANG Mingyang duan jialin DAI Lizhi XIN Xiaodong WANG Fangfang LI Zheng TIAN Ye School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105P.R.China Shenzhen Research Institute Nanjing UniversityShenzhen 518057P.R.China College of Engineering and Applied Sciences National Laboratory of Solid State MicrostructuresCollaborative Innovation Center ofAdvanced MicrostructuresJiangsu Key Laboratory of Artificial Functional MaterialsNanjing UniversityNanjing 210093P.R.China National Facility for Protein Science in Shanghai Zhangjiang LabShanghai 201210R R.China School of Physics and Optoelectronic Engineering Ludong UniversityYantai 264025P.R.China Chemistry and Biomedicine Innovation Center Nanjing UniversityNanjing 210023R R.China

DNA nanotechnology utilizes DNA double strands as building units for self-assembly of DNA *** specific base-pairing interaction between DNA molecules is the basis of these *** decades of development,this technology has been able to construct complex and programmable *** the increase in delicate nature and complexity of the synthesized nanostructures,a characterization technology that can observe these structures in three dimensions has become necessary,and developing such a technology is considerably *** assemblies have been studied using different characterization methods including atomic force microscopy(AFM),scanning electron microscopy(SEM),and transmission electron microscopy(TEM).However,the three-dimensional(3D)DNA assemblies always collapse locally due to the dehydration during the drying *** electron microscopy(cryo-EM)can overcome the challenge by maintaining three-dimensional morphologies of the cryogenic samples and reconstruct the 3D models from cryogenic samples accordingly by collecting thousands of two-dimensional(2D)projection images,which can restore their original morphologies in ***,we have reviewed several typical cases of 3D DNA-assemblies and highlighted the applications of cryo-EM in characterization of these *** comparing with some other characterization methods,we have shown how cryo-EM promoted the development of structural characterization in the field of DNA nanotechnology.

关键词： DNA nanotechnology DNA-assembly Cryogenic electron microscopy

A Novel On-Site-Real-Time Method for Identifying Characteristic Parameters Using Ultrasonic Echo Groups and Neural Network

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Chinese Journal of Mechanical Engineering 2024年第1期37卷 215-228页

作者： Shuyong duan jialin Zhang Heng Ouyang Xu Han Guirong Liu State Key Laboratory of Reliability and Intelligence of Electrical Equipment Hebei University of TechnologyTianjin 300401China Department of Aerospace Engineering and Engineering Mechanics University of CincinnatiCincinnati 45221USA

On-site and real-time non-destructive measurement of elastic constants for materials of a component in a in-service structure is a challenge due to structural complexities,such as ambiguous boundary,variable thickness,nonuniform material *** work develops for the first time a method that uses ultrasound echo groups and artificial neural network(ANN)for reliable on-site real-time identification of material *** use of echo groups allows the use of lower frequencies,and hence more accommodative to structural *** train the ANNs,a numerical model is established that is capable of computing the waveform of ultrasonic echo groups for any given set of material properties of a given *** waveform of an ultrasonic echo groups at an interest location on the surface the structure with material parameters varying in a predefined range are then computed using the numerical *** results in a set of dataset for training the ANN *** the ANN is trained,the material parameters can be identified simultaneously using the actual measured echo waveform as input to the *** tests have been conducted both numerically and experimentally to evaluate the effectiveness and accuracy of the currently proposed *** results show that the maximum identification error of numerical example is less than 2%,and the maximum identification error of experimental test is less than 7%.Compared with currently prevailing methods and equipment,the proposefy the density and thickness,in addition to the elastic ***,the reliability and accuracy of inverse prediction is significantly ***,it has broad applications and enables real-time field measurements,which has not been fulfilled by any other available methods or equipment.

关键词： Parameter identification Ultrasonic echo group High-precision modeling Artificial neural network NDT

Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro

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Journal of Traditional Chinese Medicine 2013年第1期33卷 85-91页

作者： Wei Quan Ying Yin Miaomiao Xi Dan Zhou Yanrong Zhu Yue Guan Chao Guo Yanhua Wang jialin duan Aidong Wen Department of Pharmacy Xijing HospitalFourth Military Medical University

OBJECTIVE： To determine the cardioprotective ef- fect of magnesium lithospermate B （MLB） on myo- cardial ischemia/reperfusion （MI/R） injury and to in- vestigate the antioxidant potential in vivo and in vitro. METHODS： MI/R injury was induced by the occlu- sion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GPx）, reduced glutathione （GSH） and malondialdehyde （MDA）. Blood samples were collected to determine serum levels of creatine kinase-MB （CK-MB）, cardiac troponin （cTnl） and lactate dehydrogenase （LDH）. Furthermore, simulatedischemia/reperfusion （SI/R） injury in vitro was established by oxygen and glucose deprivation （OGD） for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured su- pernatant and the levels of intracellular reactive oxygen species （ROS）, SOD and MDA in cardiomyo- cytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS： MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnl and LDH. Additional- ly, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION： MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties.

关键词： Ischemia Reperfusion injury Myocytes, Cardiac Oxidative stress Antioxidants In vitro Magnesium lithospermate B

基于R语言的AMMI模型和GGE双标图在大豆区试中的应用评价

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中国农学通报 2024年第13期40卷 140-145页

作者：张恺东张凡巧董博段佳霖陈光荣王立明杨如萍甘肃省农业科学院榆中高寒农业试验站/甘肃绿星农业科技有限责任公司兰州730070 甘肃省农业科学院旱地农业研究所兰州730700 通渭县林业和草原服务中心甘肃通渭743300 甘肃农业大学资源与环境学院兰州730070 甘肃省寒旱生态农业研究所兰州730070

为提高甘肃省大豆品种的选育和应用效率,利用大豆区域试验数据,从基因型与环境的互作分析出发,对甘肃省大豆新品种的稳定性、适应性以及各试点的鉴别力进行全面评估。本研究采用AMMI模型与GGE双标图相结合的方法对甘肃省9个大豆品种在5... 详细信息

为提高甘肃省大豆品种的选育和应用效率,利用大豆区域试验数据,从基因型与环境的互作分析出发,对甘肃省大豆新品种的稳定性、适应性以及各试点的鉴别力进行全面评估。本研究采用AMMI模型与GGE双标图相结合的方法对甘肃省9个大豆品种在5个试验点的产量进行分析,结果表明,AMMI模型中主成分值(IPCA1、IPCA2)占总变异平方和的95%;其中‘中黄318’属于高产稳产性品种,而‘陇黄3号’和‘铁丰31’虽然产量较高,但其稳定性中等,适合在特定区域栽培。在5个试验点中,凉州分辨力最强,镇原分辨力较弱。综合运用AMMI模型和GGE双标图法,能够更准确直观地反映各品种生产力、稳定性和适应能力,以及在不同试验区域的分辨能力和代表性。

关键词： AMMI模型 GGE双标图大豆稳定性适应性

Effects of Shuanghuanglian oral liquids on patients with COVID-19:a randomized,open-label,parallel-controlled,multicenter clinical trial

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Frontiers of Medicine 2021年第5期15卷 704-717页

作者： Li Ni Zheng Wen Xiaowen Hu Wei Tang Haisheng Wang Ling Zhou Lujin Wu Hong Wang Chang Xu Xizhen Xu Zhichao Xiao Zongzhe Li Chene Li Yujian Liu jialin duan Chen Chen Dan Li Runhua Zhang Jinliang Li Yongxiang Yi Wei Huang Yanyan Chen Jianping Zhao Jianping Zuo Jianping Weng Hualiang Jiang Dao Wen Wang Division of Cardiology Department of Internal Medicine and Hubei Key Laboratory of Genetics and Molecular Mechanisms of Cardiological DisordersTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China The First Affiliated Hospital of University of Science and Technology of China Hefei 230026China State Key Laboratory of Drug Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China Harbin Pharmaceutical Group Co. Ltd.Harbin 150070China The Sixth Hospital of Harbin Harbin 150036China The Second Hospital of Nanjing and the Affiliated Hospital of Nanjing University of Chinese Medicine Nanjing 210003China Department of Cardiology Chinese People’s Liberation Army Central War Command General HospitalWuhan 430010China Department of Information Management Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Division of Respiratory Department of Internal MedicineTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

We conducted a randomized,open-label,parallel-controlled,multicenter trial on the use of Shuanghuanglian(SHL),a traditional Chinese patent medicine,in treating cases of COVID-19.A total of 176 patients received SHL by three doses(56 in low dose,61 in middle dose,and 59 in high dose)in addition to standard *** control group was composed of 59 patients who received standard therapy *** with SHL was not associated with a difference from standard care in the time to disease *** with 14-day SHL treatment had significantly higher rate in negative conversion of SARS-CoV-2 in nucleic acid swab tests than the patients from the control group(93.4%vs.73.9%,P=0.006).Analysis of chest computed tomography images showed that treatment with high-dose SHL significantly promoted absorption of inflammatory focus of pneumonia,which was evaluated by density reduction of inflammatory focus from baseline,at day 7(mean difference(95%CI),−46.39(−86.83 to−5.94)HU;P=0.025)and day 14(mean difference(95%CI),−74.21(−133.35 to−15.08)HU;P=0.014).No serious adverse events occurred in the SHL *** study illustrated that SHL in combination with standard care was safe and partially effective for the treatment of COVID-19.

关键词： COVID-19 SARS-CoV-2 Shuanghuanglian oral liquid clinical trial

The serum metabolomic study of intervention effects of the traditional Chinese medicine Radix salviae miltiorrhizae and Lignum dalbergiae odoriferae in the treatment of myocardial ischemia/reperfusion injury in rats

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The serum metabolomic study of intervention effects of the t...

Anti-inflammatory Effect of Protocatechuic Aldehyde on Myocardial Ischemia/Reperfusion Injury in Vivo and in Vitro

2016年中国药学大会暨第十六届中国药师周

作者： FEI MU jialin duan HAIXU BIAN RUI LIN MEINA ZHAO AIDONG WEN YONG YANG MIAOMIAO XI Department of Pharmacy Xijing HospitalFourth Military Medical University Department of Pharmacy Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital

. Radix salviae miltiorrhizae（SM） and Lignum dalbergiae odoriferae（DO） are both traditional Chinese medicinal herbs for the treatment of ischemic heart disease and other cardiovascular diseases, but few of literature is about the systematic studies. Sprague-Dawley rats were pretreated with SM, aqueous extract of DO（DOA） and volatile oil of DO（DOO）, either as monotherapy or in combination for 7 days, then subjected to 30 min of ischemia followed by 180 min of reperfusion. Traditional pharmacodynamic evaluation and metabonomics based on gas chromatography/time-of-flight mass spectrometry（GC/TOF-MS） were used to identify the therapeutic effect of these traditional Chinese medicines. The results showed that SM, DOA and DOO ameliorated cardiac function respectively, and this effect was further strengthened by their combinations. Among all combinations, SM+DOO showed predominant potential（P＜0.01） to improve electrocardiogram and heart rate, reduce heart weight index and myocardial infarct size, decrease the levels of CK-MB and LDH. Moreover, metabonomics-based findings such as the principal component analysis（PCA） and partial least squares discriminant analysis（PLS-DA） score plot of metabolic state in rat serum correlated well to the preceding results, confirming that SM+DOO exerted synergistic therapeutic efficacies to exhibit better effect on MI/R injury rats compared with other pretreatment groups. Furthermore, the maximum effective point of SM+DOO was 30 min and the minimum effective point was 180 min. In addition, SM+DOO group markedly（P＜0.01） reduced the number of TUNEL-positive cells, the levels of TNF-α, IL-6 and MDA, while increased the activity of SOD both in serum and tissue. These results indicated that SM+DOO have combined effects that are highly effective than single pretreatment against MI/R injury rats. This effect might be achieved partly through anti-apoptosis, anti-oxidant and anti-inflammatory. Thus SM+DOO might be an effective and promising medicine f

关键词： Myocardial ischemia/reperfusion injury Radix salviae miltiorrhizae Lignum dalbergiae odoriferae The maximum and minimum effective points Cardioprotection Metabonomics

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Anti-inflammatory Effect of Protocatechuic Aldehyde on Myoca...

Antihyperglycemic,hypolipidemic and antioxidant activities of total saponins extracted from Aralia taibaiensis in experimental type 2 diabetic rats

中华医学会临床药学分会2014年全国学术会议

作者：卫国 Guan Yue Yin Ying duan jialin Zhou Dan Zhu Yanrong Quan Wei Xi Miaomiao Wen Aidong Department of Pharmacy Xijing Hospital Fourth Military Medical University

Aim The present study was aimed to investigate the anti-inflammatory effect and the possible mechanism of protocatechuic aldehyde(PAI)on MI/R injury both in vivo and in *** Pretreatment with PAI could protect the heart from MI/R injury by reducing myocardial infarct size and the activities of creatine kinase-MB(CK-MB)and cardiac troponin I(cTn-I)in ***,PAI administration markedly reduced

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Antihyperglycemic,hypolipidemic and antioxidant activities o...

中华医学会临床药学分会2014年全国学术会议

作者：翁琰 Wang YanHua Wei Guo duan jialin Yin Ying Guo Chao Zhu YanRong Guan Yue Xi MiaoMiao Wen AiDong Department of Pharmacy Xijing Hospital Fourth Military Medical University

Aim To evaluate the antihyperglycemic,hypolipidemic and antioxidant activities of total saponins extracted from Aralia taibaiensis(SAT).Methods SAT was given orally to diabetic rats at 80,160 and 320 mg/kg for 28 days to determine the antihyperglycemic *** parameters and antioxidant levels were measured to evaluate hypolipidemic and antioxidant activities

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掺铥激光在生物医疗领域的应用

激光与光电子学进展 2022年第1期59卷 42-55页

作者：张安军段嘉霖邢颍滨李进延华中科技大学武汉光电国家研究中心湖北武汉430074 华中科技大学同济医学院附属同济医院湖北武汉430074

掺铥激光凭借其可以被水分子高效率吸收的优势,成为生物组织消融和碎石手术应用的理想选择,在生物医疗上具有极大的应用前景。简要阐述了掺铥激光与生物组织作用原理,介绍了国内外掺铥激光器的最新研究成果,以及掺铥激光器在组织消融和... 详细信息

掺铥激光凭借其可以被水分子高效率吸收的优势,成为生物组织消融和碎石手术应用的理想选择,在生物医疗上具有极大的应用前景。简要阐述了掺铥激光与生物组织作用原理,介绍了国内外掺铥激光器的最新研究成果,以及掺铥激光器在组织消融和碎石手术中的应用,总结了不同激光器参数,包括工作模式、功率、照射时间、光斑面积、脉冲频率等对组织消融和碎石手术的影响,表明掺铥光纤激光器是未来医疗激光器的重要发展方向,并对国产掺铥激光器在生物医疗领域的发展提出了建议。

关键词：激光器掺铥光纤激光器激光热效应激光组织消融激光碎石