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Novel perspective in transplantation therapy of mesenchymal stem cells:targeting the ferroptosis pathway

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第2期24卷 115-129页

作者： YUZHU XU PAN FAN LEI LIU XUANFEI XU LELE ZHANG jiadong wang YUAO TAO XIAOLONG LI XI LI YUNTAO wang Medical School of Southeast University Southeast UniversityNanjing 210009China Department of Spine Surgery Zhongda Hospital Affiliated to Southeast UniversitySchool of MedicineSoutheast UniversityNanjing 210009China

Ex vivo culture-amplified mesenchymal stem cells(MSCs)have been studied because of their capacity for healing tissue injury.MSC transplantation is a valid approach for promoting the repair of damaged tissues and replacement of lost cells or to safeguard surviving cells,but currently the efficiency of MSC transplantation is constrained by the extensive loss of MSCs during the short post-transplantation period.Hence,strategies to increase the efficacy of MSC treatment are urgently needed.Iron overload,reactive oxygen species deposition,and decreased antioxidant capacity suppress the proliferation and regeneration of MSCs,thereby hastening cell death.Notably,oxidative stress(OS)and deficient antioxidant defense induced by iron overload can result in ferroptosis.Ferroptosis may inhibit cell survival after MSC transplantation,thereby reducing clinical efficacy.In this review,we explore the role of ferroptosis in MSC performance.Given that little research has focused on ferroptosis in transplanted MSCs,further study is urgently needed to enhance the in vivo implantation,function,and duration of MSCs.

关键词： Mesenchymal stem cells(MSCs) Ferroptosis Oxidative stress(OS) Iron metabolism Lipid peroxidation Glutathione peroxidase 4(GPX4)

Evolving insights:how DNA repair pathways impact cancer evolution

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Cancer Biology & Medicine 2020年第4期17卷 805-827页

作者： jiadong Zhou Xiao Albert Zhou Ning Zhang jiadong wang Department of Radiation Medicine Institute of Systems BiomedicineSchool of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China Biomedical Pioneering Irmovation Center(BIOPIC)and Translational Cancer Research Center School of Life SciencesFirst HospitalPeking UniversityBeijing 100871China Laboratory of Cancer Cell Biology Tianjin Medical University Cancer Instituteand HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China

Viewing cancer as a large,evolving population of heterogeneous cells is a common perspective.Because genomic instability is one of the fundamental features of cancer,this intrinsic tendency of genomic variation leads to striking intratumor heterogeneity and functions during the process of cancer formation,development,metastasis,and relapse.With the increased mutation rate and abundant diversity of the gene pool,this heterogeneity leads to cancer evolution,which is the major obstacle in the clinical treatment of cancer.Cells rely on the integrity of DNA repair machineries to maintain genomic stability,but these machineries often do not function properly in cancer cells.The deficiency of DNA repair could contribute to the generation of cancer genomic instability,and ultimately promote cancer evolution.With the rapid advance of new technologies,such as single-cell sequencing in recent years,we have the opportunity to better understand the specific processes and mechanisms of cancer evolution,and让s relationship with DNA repair.Here,we review recent findings on how DNA repair affects cancer evolution,and discuss how these mechanisms provide the basis for critical clinical challenges and therapeutic applications.

关键词： DNA repair cancer evolution intratumor heterogeneity genomic instability drug resistance

Maintenance of Genome Stability

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Genomics, Proteomics & Bioinformatics 2016年第3期14卷 119-121页

作者： jiadong wang Tomas Lindahl Institute of Systems Biomedicine Department of Radiation MedicineSchool of Basic Medical SciencesPeking University Cancer Research UK London Research Institute Clare Hall Laboratories

It was ever thought that genomic information is transmitted faithfully from generation to generation. But our current knowledge does not indicate that it is the case. For example, genomic variations can be generated from DNA replication infidelity and unequal chromosome segregation. Natural decay of DNA molecules is also a fundamental source of changing genomic information. In addition, cellular and organismal exposure to exogenous genotoxic agents such as ultraviolet （UV） light, oxidative stress, chemical mutagens, and radiation can lead to a variety of modifications on DNA constituents, resulting in genome alterations. Fortunately, cells have evolved several response systems to tackle numerous DNA lesions in order to maintain their genome integrity. Among them, check- point control is probably the most well-known one. For exam- ple, checkpoint responds to replication stress, replication fork stalling, double-strand DNA breaks, and various other types of DNA lesions. Increasing experimental evidence indicates that genomic instability is probably the fundamental reason for carcinogenesis. Genomic instability is also found to be a main etiological factor of neurodegenerative diseases, aging, immunodeficiency, etc. Thus, to understand how cells regulate to maintain their genomic stability is of fundamental importance.

关键词： genomic Genome chromosome DNA segregation replication decay eukaryotic ultraviolet transmitted

粒子滤波和GRU神经网络融合的锂电池RUL预测

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哈尔滨工业大学学报 2024年第5期56卷 142-151页

作者：贺宁张思媛李若夏高峰王家栋西安建筑科技大学机电工程学院西安710055 西安建筑科技大学信息与控制工程学院西安710055 浙江中控技术股份有限公司杭州310000

随着锂电池在移动电子设备和电动汽车等领域中得到广泛应用,其剩余使用寿命(remaining useful life,RUL)的精确预测对锂电池的健康管理更具重要意义。本文提出一种基于粒子滤波(particle filter,PF)和门控循环单元(gated recurrent unit... 详细信息

随着锂电池在移动电子设备和电动汽车等领域中得到广泛应用,其剩余使用寿命(remaining useful life,RUL)的精确预测对锂电池的健康管理更具重要意义。本文提出一种基于粒子滤波(particle filter,PF)和门控循环单元(gated recurrent unit,GRU)神经网络融合(PF-GRU)的预测方法预测锂电池的RUL。这种融合方法结合了PF在估计RUL概率分布上的优势以及GRU能够进行时间序列长期预测的能力,获得融合的预测结果。再利用每个预测周期的容量预测结果,采用带移动窗口迭代更新训练数据集的方法对GRU模型进行再训练,提高了GRU的长期预测性能。以上预测步骤迭代进行,直到容量衰减至寿命阈值以下。最后将粒子代表的预测结果外推至寿命阈值,得到电池RUL分布直方图。本文采用美国NASA卓越诊断学中心(PCoE)实验室所提供的锂电池数据对所提方法进行验证,将所提出的融合方法与GRU、PF和无窗口移动融合方法进行RUL预测比较。实验结果表明,本文所提出的融合方法具有良好的性能,在状态和参数估计、RUL预测方面明显优于PF和无窗口移动融合方法,预测精度均高于其他3种预测方法。

关键词：锂电池剩余使用寿命粒子滤波 GRU神经网络融合方法

两级变幅应变疲劳本征损伤耗散寿命预测模型研究

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力学学报 2024年第1期56卷 149-156页

作者：李文奇向忠李浩然任忠凯王嘉栋浙江理工大学机械工程学院杭州310018 太原理工大学机械与运载工程学院太原030024

在变幅低周疲劳载荷条件下,材料的疲劳损伤累积过程中呈现出了显著的载荷顺序效应.这种现象复杂化了变幅疲劳寿命的预测,因此,如何更精确地预测变幅低周载荷下的疲劳寿命,已经成为了一个亟待解决的问题.针对变幅低周疲劳载荷下损伤累积... 详细信息

在变幅低周疲劳载荷条件下,材料的疲劳损伤累积过程中呈现出了显著的载荷顺序效应.这种现象复杂化了变幅疲劳寿命的预测,因此,如何更精确地预测变幅低周载荷下的疲劳寿命,已经成为了一个亟待解决的问题.针对变幅低周疲劳载荷下损伤累积表现的载荷顺序效应,考虑到本征损伤耗散与疲劳损伤构成一一映射,且刻画低周疲劳破坏的热力学本质,基于连续介质损伤力学及其不可逆热力学框架,推导本征损伤耗散功演化模型D型描述并以等同本征损伤耗散功作为损伤转换条件,建立了一种考虑载荷顺序效应的变幅低周疲劳寿命预测模型.为了验证新模型的有效性和先进性,进行了P355NL1结构钢和Ti-6Al-4V钛合金在两级变幅载荷下的单轴低周疲劳实验验证,并与Manson模型、Kwofie模型和Peng模型进行了比较分析.研究结果表明,新建模型的预测效果都在1.5倍的误差范围内,与实验结果相当吻合,且优于现有的预测模型.运用本征损伤耗散理论开展变幅疲劳寿命预测,为金属材料变幅疲劳寿命预测提供了新思路.

关键词：低周疲劳载荷顺序效应损伤力学本征损伤耗散寿命预测

Inhibition of the toxic byproduct during photocatalytic NO oxidation via La doping in ZnO

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Chinese Chemical Letters 2020年第3期31卷 751-754页

作者： Chaowei Yuan Wen Cui Yanjuan Sun jiadong wang Ruimin Chen Jin Zhang Yuxin Zhang Fan Dong Chongqing Key Laboratory of Catalysis and New Environmental Materials College of Environment and ResourcesChongqing Technology and Business UniversityChongqing 400067China Research Center for Environmental Science&Technology Institute of Fundamental and Frontier SciencesUniversity of Electronic Science and Technology of ChinaChengdu 611731China The Center of New Energy Materials and Technology School of Materials Science and EngineeringSouthwest Petroleum UniversityChengdu 610500China College of Materials Science and Engineering Chongqing UniversityChongqing 400044China

It is of a great challenge to develop semiconductor photocatalysts with potential possibilities to simultaneously enhance photocatalytic efficiency and inhibit generation of toxic intermediates.In this study,we developed a facile method to induce the La doping and cationic vacancie(V(Zn))on ZnO for the highly efficient complete NO oxidation.The photocatalytic NO removal efficiency increases from 36.2%to 53,6%.Most importantly,a significant suppressed NO2 production also has been realized.According to the DFT calculations,ESR spectra and in situ FTIR spectra,the introduction of La^3+induce the redistribution of charge carriers in La-ZnO,which promote the production of·O2^- and lead to the formation of V(Zn)for the formation of·OH,contributing to the complete oxidation of NO to nitrate.Besides,the conversion pathway of photocatalytic NO oxidation has been elaborated,This work paves a new way to simultaneously realize the photocatalytic pollutants removal and the inhibition of toxic intermediates generation for efficient and safe air purification.

关键词： Photocatalytic NO oxidation Toxic byproduct Reactive oxygen species ZnO Cationic vacancie

高功率Tesla变压器次级绝缘结构电场分析与设计

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高压电器 2024年第5期60卷 84-91页

作者：蔡宛辰金童沈骏峰王佳栋江进波三峡大学电气与新能源学院湖北宜昌443002 国网台州供电公司浙江台州318000 国网衢州供电公司浙江衢州324000 三峡大学湖北省输电线路工程技术研究中心湖北宜昌443002

高功率Tesla变压器在脉冲功率技术领域应用广泛,但其次级线圈处于非均匀电场中,线圈匝间电压分布不均,容易导致匝间绝缘击穿。文中设计了一台双磁心Tesla变压器,利用有限元法建立次级线圈的电场仿真模型,分析其匝间的电场分布情况。针... 详细信息

高功率Tesla变压器在脉冲功率技术领域应用广泛,但其次级线圈处于非均匀电场中,线圈匝间电压分布不均,容易导致匝间绝缘击穿。文中设计了一台双磁心Tesla变压器,利用有限元法建立次级线圈的电场仿真模型,分析其匝间的电场分布情况。针对匝间电压分布不均问题,提出次级线圈首尾两端双线并绕及增加屏蔽环来改善电场分布的措施,使最大场强小于微小油间隙的击穿场强,确保变压器次级线圈的绝缘安全。通过仿真分析发现首尾两端并绕可改善电场分布,但并绕匝数不宜过多。高压端增加屏蔽环后,场强分布更加均匀,最大场强下降62.9%,且最大场强远低于脉冲条件下的油间隙击穿场强,能够满足绝缘设计要求。

关键词：高功率Tesla变压器电场分布双线并绕屏蔽环绝缘设计

六神曲发酵过程挥发性风味成分的变化及中药组分缺失对其风味的影响

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中国酿造 2024年第1期43卷 222-229页

作者：殷娴曹爽郭慧敏王家栋廖永红北京工商大学轻工科学技术学院北京100048 北京工商大学北京食品营养与人类健康高精尖创新中心北京100048

六神曲是自然发酵的中药曲剂,气味是监控六神曲发酵过程和评判质量的重要指标。该实验采用顶空固相微萃取-气质联用(HS-SPME-GC-MS)技术分析六神曲的挥发性风味成分,探究发酵过程挥发性风味物质的变化规律及中药组分缺失对其挥发性风味... 详细信息

六神曲是自然发酵的中药曲剂,气味是监控六神曲发酵过程和评判质量的重要指标。该实验采用顶空固相微萃取-气质联用(HS-SPME-GC-MS)技术分析六神曲的挥发性风味成分,探究发酵过程挥发性风味物质的变化规律及中药组分缺失对其挥发性风味成分的影响。结果表明,在六神曲发酵过程中共检测出33种挥发性风味物质,其中2种酸类、6种醇类、7种酯类、3种醛类、11种烃类、1种酮类和3种其他类。六神曲在发酵0 h时挥发性风味物质以苯甲醛为主,含量为2157.43μg/mL。发酵过程中,苯甲醛含量迅速下降,72 h时含量降至116.15μg/mL,而苯甲醇、醋酸和苯乙酸甲酯等物质的含量明显上升,发酵结束时含量分别为703.95μg/mL、252.97μg/mL和143.12μg/mL。当中药组分缺失青蒿、辣蓼、苍耳草和苦杏仁中的任何一种,六神曲的挥发性风味物质发生极大变化,发酵过程中苯甲醛含量下降速度均减缓,而苯甲醇、醋酸和苯乙酸甲酯含量的增长速度亦降低;其中,原料缺少苦杏仁时的苯甲醛初始含量仅为101.73μg/mL,苦杏仁是苯甲醛的产生的主要因素。

关键词：六神曲发酵中药组分固相微萃取-气相色谱-质谱挥发性风味物质

Eupatilin通过Sesn2-Nrf2保护线粒体功能在脓毒症脑损伤中的作用

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实用医学杂志 2024年第5期40卷 601-607页

作者：王加栋黄方舟黄艳陈管雄刘军黄佩琦长沙市第四医院(湖南师范大学附属长沙医院)急诊急救中心长沙410006

目的探讨Sestrin2(Sesn2)的保护线粒体功能在减轻脓毒症脑损伤(SAE)小鼠的认知功能障碍中的作用研究。方法 120只6周大的雄性C57BL/6J小鼠随机分为3组,每组40只:假手术(Sham)组、CLP组、CLP+Eupatilin组。建立由盲肠结扎和穿孔(CLP)手... 详细信息

目的探讨Sestrin2(Sesn2)的保护线粒体功能在减轻脓毒症脑损伤(SAE)小鼠的认知功能障碍中的作用研究。方法 120只6周大的雄性C57BL/6J小鼠随机分为3组,每组40只:假手术(Sham)组、CLP组、CLP+Eupatilin组。建立由盲肠结扎和穿孔(CLP)手术诱导脓毒症模型。CLP+Eupatilin组小鼠采用Eupatilin治疗。通过神经行为测试、Morris水迷宫(MWM)来确定小鼠神经行为、空间学习和记忆功能。通过尼氏染色法计数海马CA1区的神经元数目。将HT22细胞随机分为对照组(Con)、脂多糖组(LPS)、LPS+Eupatilin组、LPS+Eupatilin+si-Nrf2组。通过TUNEL染色分析细胞凋亡,和线粒体膜电位(MMP)分析线粒体损伤。结果在CLP手术后7 d,与Sham小鼠相比,CLP小鼠的海马和皮质中Sesn2显著下调(P <0.01)。与CLP组相比,CLP+Eupatilin组的存活率显著增加(P <0.05)。与Sham组相比,CLP组小鼠表现出相对较高的神经损伤评分(P <0.05),和具有更少的平台穿越次数和更短的目标停留时间,而CLP+Eupatilin组中小鼠神经损伤评分较CLP组显著降低(P <0.05),并且停留在目标区域时间和平台穿越次数显著高于CLP组(P <0.05)。与Sham组相比,CLP组小鼠海马组织中神经元、Sesn2和Nrf2的共定位率明显减少(P <0.05),和CD68/Iba-1阳性小胶质细胞数量显著增加(P <0.05),而CLP+Eupatilin组逆转了这些变化。与Con组相比,LPS组细胞凋亡和MMP水平显著增加(P <0.01),而LPS+Eupatilin组细胞凋亡和MMP水平显著低于LPS组(P <0.05)。然而,Nrf2敲低(LPS+Eupatilin+si-Nrf2组)逆转了Eupatilin的抗细胞凋亡作用和线粒体保护作用。结论 Eupatilin通过激活Sesn2-Nrf2通路减轻SAE小鼠的认知功能障碍、神经功能缺损,并且通过减轻线粒体功能障碍来改善炎症微环境。

关键词： Sestrin2 脓毒症脑损伤线粒体认知功能障碍核因子红系-2相关因子2