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Mid-infrared chemical imaging of intracellular tau fibrils using fluorescence-guided computational photothermal microscopy

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Light(Science & Applications) 2023年第7期12卷 1354-1369页

作者： Jian Zhao Lulu Jiang Alex Matlock Yihong Xu jiabei zhu Hongbo zhu Lei Tian Benjamin Wolozin Ji-xin Cheng Department of Electrical and Computer Engineering Boston UniversityBostonMA 02215USA The Picower Institute for Learning and Memory Massachusetts Institute of TechnologyCambridgeMA 02142USA Department of Pharmacology and Experimental Therapeutics Boston University School of MedicineBostonMA 02118USA Department of Physics Boston UniversityBostonMA 02215USA State Key Laboratory of Luminescence and Applications Changchun Institute of OpticsFine Mechanics and PhysicsChinese Academy of Sciences130033 ChangchunChina Department of Biomedical Engineering Boston UniversityBostonMA 02215USA photonics Center Boston UniversityBostonMA 02215USA present address:Department of Mechanical Engineering Massachusetts Institute of TechnologyCambridgeMA 02142USA

Amyloid proteins are associated with a broad spectrum of neurodegenerative ***,it remains a grand challenge to extract molecular structure information from intracellular amyloid proteins in their native cellular *** address this challenge,we developed a computational chemical microscope integrating 3D midinfrared photothermal imaging with fluorescence imaging,termed Fluorescence-guided Bond-Selective Intensity Diffraction Tomography(FBS-IDT).Based on a low-cost and simple optical design,FBS-IDT enables chemical-specific volumetric imaging and 3D site-specific mid-IR fingerprint spectroscopic analysis of tau fbrils,an important type of amyloid protein aggregates,in their intracellular ***-free volumetric chemical imaging of human cells with/without seeded tau fibrils is demonstrated to show the potential correlation between lipid accumulation and tau aggregate ***-resolved mid-infrared fingerprint spectroscopy is performed to reveal the protein secondary structure of the intracellular tau fibrils.3D visualization of theβ-sheet for tau fibril structure is achieved.

关键词： tau amyloid chemical

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Global profiling of O-GlcNAcylated and/or phosphorylated proteins in hepatoblastoma

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Signal Transduction and Targeted Therapy 2019年第1期4卷 311-319页

作者： Hang Song Ji Ma Zhixuan Bian Shuhua Chen jiabei zhu Jing Wang Nan Huang Minzhi Yin Fenyong Sun Min Xu Qiuhui Pan Department of Laboratory Medicine Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiaotong University200127 ShanghaiChina Department of Laboratory Medicine Yunfu People’s Hospital527300 GuangdongChina Department of Surgery Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiaotong University200127 ShanghaiChina Department of Laboratory Medicine Shanghai Tenth People’s Hospital of Tongji University200072 ShanghaiChina Department of Pathology Shanghai Children’s Medical CenterSchool of MedicineShanghai Jiaotong University200127 ShanghaiChina

O-linked-β-N-acetylglucosamine(O-GlcNAc)glycosylation(O-GlcNAcylation)and phosphorylation are critical posttranslational modifications that are involved in regulating the functions of proteins involved in tumorigenesis and the development of various solid ***,a detailed characterization of the patterns of these modifications at the peptide or protein level in hepatoblastoma(HB),a highly malignant primary hepatic tumor with an extremely low incidence in children,has not been ***,we examined O-GlcNAc-modified or phospho-modified peptides and proteins in HB through quantitative proteomic analysis of HB tissues and paired normal liver *** results identified 114 O-GlcNAcylated peptides belonging to 78 proteins and 3494 phosphorylated peptides in 2088 ***,41 proteins were modified by both O-GlcNAcylation and *** proteins are involved in multiple molecular and cellular processes,including chromatin remodeling,transcription,translation,transportation,and organelle *** addition,we verified the accuracy of the proteomics results and found a competitive inhibitory effect between O-GlcNAcylation and phosphorylation of ***,O-GlcNAcylation modification of HSPB1 promoted proliferation and enhanced the chemotherapeutic resistance of HB cell lines in ***,our research suggests that O-GlcNAc-modified and/or phospho-modified proteins may play a crucial role in the pathogenesis of HB.

关键词： hepato competitive modification

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Ginsenoside Rg1 impairs homologous recombination repair by targeting CtIP and sensitizes hepatoblastoma cells to DNA damage agents

Ginsenoside Rg1 impairs homologous recombination repair by t...

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第一届中国临床分子诊断大会

作者： Ni Zhen Ji Ma jiabei zhu Qiuhui Pan Shanghai Children’s Medical Center

＜正＞Background:The ginsenoside Rg1,the primarypharmacologically active ingredient of the traditional Chinese herb ginseng,is widely used in the clinical treatment of diseases of the immune and nervous *** studies have shown that it also has an antitumor ***:In this study,we explored the effects of Rgl on hepatoblastoma （HB） and its underlying ***:MTT assays,colony formation assays,

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Anatomical Modeling of Brain Vasculature in Two-Photon Microscopy by Generalizable Deep Learning

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Biomedical Engineering Frontiers 2021年第1期2卷 103-114页

作者： Waleed Tahir Sreekanth Kura jiabei zhu Xiaojun Cheng Rafat Damseh Fetsum Tadesse Alex Seibel Blaire S.Lee Frédéric Lesage Sava Sakadžic David A.Boas Lei Tian Department of Electrical and Computer Engineering Boston UniversityBostonMAUSA Department of Biomedical Engineering Boston UniversityBostonMAUSA Biomedical Engineering Institute École Polytechnique de MontréalMontréalQCCanada Institute of Neurological Sciences and Psychiatry Hacettepe UniversityAnkaraTurkey Department of Radiology Massachusetts General HospitalHarvard Medical SchoolCharlestownUSA Neurophotonics Center Boston UniversityBostonMAUSA

Objective and Impact *** of blood vessels from two-photon microscopy(2PM)angiograms of brains has important applications in hemodynamic analysis and disease ***,we develop a generalizable deep learning technique for accurate 2PM vascular segmentation of sizable regions in mouse brains acquired from multiple 2PM *** technique is computationally efficient,thus ideal for large-scale neurovascular *** segmentation from 2PM angiograms is an important first step in hemodynamic modeling of brain *** segmentation methods based on deep learning either lack the ability to generalize to data from different imaging systems or are computationally infeasible for large-scale *** this work,we overcome both these limitations by a method that is generalizable to various imaging systems and is able to segment large-scale *** employ a computationally efficient deep learning framework with a loss function that incorporates a balanced binary-cross-entropy loss and total variation regularization on the network’s *** effectiveness is demonstrated on experimentally acquired in vivo angiograms from mouse brains of dimensions up to 808×808×702μ*** demonstrate the superior generalizability of our framework,we train on data from only one 2PM microscope and demonstrate high-quality segmentation on data from a different microscope without any network ***,our method demonstrates 10×faster computation in terms of voxels-segmented-per-second and 3×larger depth compared to the *** work provides a generalizable and computationally efficient anatomical modeling framework for brain vasculature,which consists of deep learning-based vascular segmentation followed by *** paves the way for future modeling and analysis of hemodynamic response at much greater scales that were inaccessible before.

关键词： Deep network overcome

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MiroRNA-127-3p targets XRCC3 to enhance the chemosensitivity of hepatoblastoma cells to a novel phenanthroline-dione derivative

MiroRNA-127-3p targets XRCC3 to enhance the chemosensitivity...

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第一届中国临床分子诊断大会

作者： Ni Zhen Ji Ma jiabei zhu Qiuhui Pan Shanghai Children’s Medical Center

＜正＞Purpose:The story mainly aims to determine the biological functions of microRNA-127-3p in hepatoblastoma carcinogenesis and to explore its potential ***:In the study,we used MTT assays,colony formation assays,tumor-bearing nude mice to analyze the roles of microRNA-127-3p in regulating the growth of hepatoblastoma cells both in vitro and in vivo.

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钙碳比对CaSO_4-CaCO_3-(NH_4)_2CO_3-H_2O体系反应-结晶过程的影响

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化工学报 2018年第6期69卷 2533-2539页

作者：宫源周加贝朱家骅罗安安薛潇田建四川大学化学工程学院四川成都610065

在CaSO_4-CaCO_3-(NH_4)_2CO_3-H_2O反应体系中,生成碳酸钙与硫酸铵的控速步骤是石膏溶解。溶解表面被新生的碳酸钙结晶覆盖是重要影响因素。基于反应面模型,通过调整钙离子溶出速率与碳酸根加入速率之比(钙碳比),将反应-结晶面控制在... 详细信息

在CaSO_4-CaCO_3-(NH_4)_2CO_3-H_2O反应体系中,生成碳酸钙与硫酸铵的控速步骤是石膏溶解。溶解表面被新生的碳酸钙结晶覆盖是重要影响因素。基于反应面模型,通过调整钙离子溶出速率与碳酸根加入速率之比(钙碳比),将反应-结晶面控制在溶解表面的固液扩散边界层临界距离之外从而避免覆盖。实验采用表面积一定的天然纯净块状石膏试样,并向溶解体系滴加碳酸铵溶液,使钙碳比分别为0.125、0.25、0.5、1、2、4和8,观察碳酸钙在石膏表面的包覆现象,并预测反应-结晶面与溶解表面之间的微距离。结果显示,当钙碳比为0.125、0.25、0.5和1时,试样表面出现碳酸钙包覆但包覆量随λ增大而减小;当钙碳比为2、4和8时,试样表面不出现包覆,碳酸钙结晶生成于液相主体。将钙碳比为1视为临界值,该条件下反应-结晶面与溶解表面的微距离大于18μm。

关键词：反应结晶包覆钙碳比二氧化碳捕集

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NeuPh:scalable and generalizable neural phase retrieval with local conditional neural fields

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Advanced Photonics Nexus 2024年第5期3卷 67-76页

作者： Hao Wang jiabei zhu Yunzhe Li Qianwan Yang Lei Tian Boston University Department of Electrical and Computer EngineeringBostonMassachusettsUnited States Boston University Department of Biomedical EngineeringBostonMassachusettsUnited States

Deep learning has transformed computational imaging,but traditional pixel-based representations limit their ability to capture continuous multiscale object *** this gap,we introduce a local conditional neural field(LCNF)framework,which leverages a continuous neural representation to provide flexible object ***’s unique capabilities are demonstrated in solving the highly ill-posed phase retrieval problem of multiplexed Fourier ptychographic *** network,termed neural phase retrieval(NeuPh),enables continuous-domain resolution-enhanced phase reconstruction,offering scalability,robustness,accuracy,and generalizability that outperform existing *** integrates a local conditional neural representation and a coordinate-based training *** show that NeuPh can accurately reconstruct high-resolution phase images from low-resolution intensity ***,NeuPh consistently applies continuous object priors and effectively eliminates various phase artifacts,demonstrating robustness even when trained on imperfect ***,NeuPh improves accuracy and generalization compared with existing deep learning *** further investigate a hybrid training strategy combining both experimental and simulated datasets,elucidating the impact of domain shift between experiment and *** work underscores the potential of the LCNF framework in solving complex large-scale inverse problems,opening up new possibilities for deep-learning-based imaging techniques.

关键词： neural representation phase retrieval computational imaging deep learning computational microscopy

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New insight into the origin and evolution of the flora and fauna of Hainan Island, China

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Science China Earth Sciences 2025年第1期68卷 52-61页

作者： Hua zhu Center for Integrative Conservation Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences Yunnan International Joint Laboratory of Southeast Asia Biodiversity Conservation Yunnan Key Laboratory for the Conservation of Tropical Rainforests and Asian Elephants

The origin of the biota of Hainan Island, China, remains a fundamental scientific problem. Comparative studies on the biota, geological tectonics, and paleomagnetism proposed that during the Eocene, Hainan Island connected Vietnam and Guangxi, and then moved to the southeast to reach its present location. This review mainly discusses its biota origin from molecular phylogeny and palaeobotanical research. The formation and evolution of the biological groups of Hainan Island were identified via research on their genomes, phylogeny, and molecular biogeography. This research showed that the genera and species that appeared and diverged early in their evolution(i.e., before the Miocene) can be explained by the land connection between Hainan Island, Vietnam, and Guangxi during the Eocene-Oligocene. According to Southeast Asian tectonic theory, in the Middle Miocene(at about 15 million years ago), Hainan Island had moved to its present position. The population and genetic divergence of species with late divergence times(after 15 Ma) can be reasonably explained by the close proximity(or land connection) between Hainan Island and the Leizhou Peninsula, enabling genetic communication between Hainan Island and the Chinese mainland, but have exceptional cases. Conclusions have been obtained through palaeobotanical studies, showing that in the Eocene-Oligocene period, southwest China and Hainan Island had subtropical vegetation and flora. However, the fossil group in southeast China(in Maoming, near present-day Hainan Island) in the Late Eocene indicated a tropical rainforest vegetation and flora. This suggests that in the Paleogene, Hainan Island may have been located near or on the southwest border of Guangxi and northern Vietnam, rather than in its present location. The floristic division of the flora of Hainan Island basically presents a northeast-southwest inclined distribution pattern. This pattern can be explained by the counterclockwise rotation of Hainan Island during i

关键词： Molecular phylogeny Biogeography Paleobotany Origin of flora and fauna Hainan Island of China

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Modification of Maxwell model for conductivity prediction of carbon nanotubes-filled polymer composites with tunneling effect

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Applied Mathematics and Mechanics(English Edition) 2025年第1期46卷 25-36页

作者： Jue zhu Longyuan LI Ningtao zhu Zhejiang Provincial Engineering Research Center for the Safety of Pressure Vessel and Pipeline Key Laboratory of Impact and Safety Engineering (Ningbo University) Ministry of Education School of Engineering Computing and Mathematics University of Plymouth Xi'an Zhanshi Testing & Engineering Co.Ltd.

Carbon nanotubes(CNTs) have garnered great attention in recent years due to their outstanding electrical, thermal, and mechanical properties. The incorporation of small amounts of CNTs in polymers can substantially improve the sensitivity of the polymer's electrical conductivity. This paper presents a modified Maxwell model to evaluate the electrical conductivity of CNTs-filled polymer composites by introducing a transition zone to account for the tunneling effect. In this modified Maxwell model, the CNTs-filled polymer composite is modeled as a three-phase composite, consisting of a matrix(polymer), inclusions(CNTs), and a transition zone(tunneling zone). The effective electrical conductivity(EEC) of the composite is calculated based on the volume fractions and electrical conductivities of the matrix, inclusions, and transition zone. The model's validity is confirmed through the use of available test data, which demonstrates its capability to accurately capture the nonlinear conductivity behavior observed in CNTs-polymer composites. This study offers valuable insights into the design of high-performance conductive polymer nanocomposites, and enhances the understanding of electrical conduction mechanisms in CNT-dispersed polymer composites.

关键词： Nanoclay

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Late Paleozoic to early Mesozoic volcanic-sedimentary history of the southeastern margin of the Central Asian Orogenic Belt: Timing of uplift of the Inner Mongolia paleo-uplift

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Science China Earth Sciences 2025年第1期68卷 297-309页

作者： Wenxing HAO Jin-Hui YANG Guang zhu State Key Laboratory of Lithospheric Evolution Institute of Geology and Geophysics Chinese Academy of Sciences School of Resource and Environmental Engineering Hefei University of Technology

The Kulun region is located in the eastern Bainaimiao arc belt on the southeastern margin of the Central Asian Orogenic Belt(CAOB), adjacent to the North China Craton(NCC) to the south. This region records the closure of the PaleoAsian Ocean during the Paleozoic to early Mesozoic and is key to study the tectonic evolution of the northern margin of the *** conducted zircon U-Pb geochronology and Hf isotope analysis of volcanic-sedimentary and intrusive rocks in the Kulun region. The volcanic-sedimentary rocks were previously assigned an early Carboniferous age, but include volcanic rocks with ages of 267–266 Ma and sedimentary rocks with youngest detrital zircon age peaks of 265–244 Ma, which were intruded by a granite with an age of 242 Ma. This indicates that these rocks are actually middle Permian to Middle Triassic in age(267–242Ma) and correspond to the Qingfengshan and Liujiagou formations. Moreover, two volcanic rock samples have high εHf(t) values(+1.6 to +16.1) and relatively young two-stage Hf model ages(1095–327 Ma), indicating their sources associated with the CAOB. The age spectra and Hf isotopic characteristics of detrital zircons from the sedimentary rocks suggest that, during the middle Permian to Middle Triassic, the sedimentary provenance in the Kulun region was mainly the CAOB. However, by the Early Jurassic, the provenance changed to the NCC(i.e., the Inner Mongolia Paleo-uplift, IMPU). This result, combined with the development of a series of conglomerates on the northern margin of the NCC in the Late Triassic(i.e., Xingshikou, Xiaoyingzi,and Xiaohekou formations), indicates the Kulun region experienced an abrupt change in paleogeography during the Late Triassic, with the IMPU undergoing significant uplift and thus becoming the main sediment source in the Kulun region. Based on the Late Triassic alkaline igneous belt(235–215 Ma) in the IMPU, we speculate that this uplift was caused by detachment of the southward-subducting Paleo-Asian oceanic

关键词： Central Asian Orogenic Belt the North China Craton Zircon geochronology Hf isotopes Inner Mongolia Paleo-uplift

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