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Single-cell transcriptomic profiling reveals ZEB1-mediated regulation in microglial subtypes and the impact of exercise on neuroinflammatory responses

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Traditional Medicine Research 2025年第2期10卷 55-64页

作者： Jin-Fang Wu yi-Sheng chen Yu-Chun Xie Department of Rehabilitation Medicine Jiangsu Province Geriatric HospitalNanjing 210024China Fujian Key Laboratory of Toxicant and Drug Toxicology Medical CollegeNingde Normal UniversityNingde 352100China Central Laboratory Jiangsu Province Geriatric HospitalNanjing 210024China

Background:This study aims to identify distinct cellular subtypes within brain tissue using single-cell transcriptomic analysis,focusing on specific biomarkers that differentiate cell types and the effects of traditional and exercise ***:Four samples were analyzed:older control(OC),older exercise(OE),younger control(YC),and younger exercise(YE).Single-cell RNA sequencing was used to distinguish cellular subtypes through their biomarker *** visualization included violin and t-SNE plots to illustrate biomarker expression across cell clusters such as oligodendrocytes,microglia,and ***,BV2 cells were exposed to amyloid-beta fragments to simulate Alzheimer’s disease,assessing the impact of exercise-induced cellular ***:Distinct cellular subtypes were identified:oligodendrocytes(MBP,St18),microglia(Dock8),and astrocytes(Aqp4,Gpc5).Sample OE was predominantly oligodendrocytes,while YE had more astrocytes,inhibitory neurons,and Canal-Retzius *** showed a significant presence of Olfm3+ganglion ***1 gene knockout revealed changes in SMAD family gene expression,which regulate *** stress levels were also ***:This profiling enhances our understanding of brain cellular functions and interactions,potentially informing targeted therapies in neurological *** may influence brain cell immune responses and cell death pathways by regulating specific gene expressions,offering new insights for treating neuroinflammation and degeneration.

关键词： single-cell transcriptomics brain tissue cellular subtypes biomarker expression neuronal diversity

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Insufficient MIRO1 contributes to declined oocyte quality during reproductive aging

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Science China Life Sciences 2025年

作者： Zhen-Nan Pan Hao-Lin Zhang Kun-Huan Zhang jia-Qian Ju Jing-Cai Liu Shao-chen Sun College of Animal Science and Technology Nanjing Agricultural University Reproductive Medicine Centre The Affiliated Yantai Yuhuangding Hospital of Qingdao University

Mitochondrial Rho-GTPase 1 (MIRO1) is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis. In present study, we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging. We found that MIRO1 expressed in mouse and porcine oocytes, and its expression decreased in aged mice. MIRO1 deficiency caused the failure of meiotic resumption and polar body extrusion in both mouse and porcine oocytes, which could be rescued by exogenous MIRO1supplementation. Mass spectrometry data indicated that MIRO1 associated with several cytoskeleton and cell cycle-related proteins, and MIRO1 regulated motor protein Dynein for microtubule-organizing centers (MTOCs) dynamics at germinal vesicle (GV) stage, which determined meiotic resumption. Furthermore, we found that MIRO1 regulated Aurora A and kinesin family member 11 (KIF11) for meiotic spindle assembly in oocytes. Besides, MIRO1 associated with several mitochondria-related proteins dynamic-related protein 1 (DRP1),Parkin and lysosomal-associated membrane protein 2 (LAMP2) for mitochondrial dynamics and mitophagy during oocyte meiosis. Taken together, our results suggested that MIRO1 played pivotal roles in meiotic resumption, spindle assembly and mitochondrial function in mouse and porcine oocytes, and its insufficiency might contribute to the oocyte maturation defects during aging.

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超声/微波辅助提取分离钛铁矿中的钛铁

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有色金属（冶炼部分） 2017年第3期 24-26页

作者：滕家辉高一陈煜张凯龙浙江万里学院生物与环境学院浙江宁波315100

采用超声、微波辅助硫酸酸解—水浸的方法从钛铁矿中分离钛铁。结果表明,容量分析法可以准确测定钛铁提取液中钛铁含量,超声、微波辅助硫酸分解—水浸出取效率显著,水浸10min达到稳定,钛铁浸出率分别达到97.2%和98.0%。

关键词：钛铁矿硫酸超声微波

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平面细胞极性在晶状体发育中的作用

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国际眼科杂志 2019年第12期19卷 2041-2044页

作者：陈奕嘉李金燕欧阳帅罗莉霞中山大学中山眼科中心眼科学国家重点实验室

晶状体发育是一个复杂的生物学过程,受多种信号分子及其构成的通路网络调控。近年研究发现,平面细胞极性(PCP)信号通路在晶状体发育过程中起重要作用,是形成晶状体正常透明度和形态的基础。PCP的深入研究为临床治疗先天性白内障提供了... 详细信息

晶状体发育是一个复杂的生物学过程,受多种信号分子及其构成的通路网络调控。近年研究发现,平面细胞极性(PCP)信号通路在晶状体发育过程中起重要作用,是形成晶状体正常透明度和形态的基础。PCP的深入研究为临床治疗先天性白内障提供了指导意义,同时更是有望成为完善再生晶状体的新干预靶点。本文结合目前该领域研究进展就PCP在晶状体的发育过程中的作用进行详细综述。

关键词：平面细胞极性晶状体发育 Wnt/PCP信号通路晶状体纤维细胞细胞骨架

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Facile synthesis of the dodecahydridododecaborate (B12H122-) from borane Lewis base adducts

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Science China Chemistry 2025年

作者： yi Jing Xin-Ran Liu Xing-Hua Wang Hui Han Sha-Sha Liang Xi-Meng chen Dong-Hui Wei Lai-Sheng Wang Xue-Nian chen College of Chemistry Zhengzhou University School of Chemistry and Chemical Engineering Henan Key Laboratory of Boron Chemistry and Advanced MaterialsHenan Normal University Department of Chemistry Brown University

Polyhedral boranes are a class of well-known boron molecular clusters widely used in energy,chemistry,medicine,and materials science because of their unique physical and chemical *** efforts have been made in the past decades to find more effective synthetic methods for this important class of boron ***,existing synthetic methods suffer from low efficiency and low ***,we report a facile one-pot synthesis of[（CH3）3S]2B12H12with moderate yields at mild *** mechanisms for the multi-step chemoselective synthesis of B12H122-without other by-products are elucidated based on theoretical results and our previous *** Lewis base used in B–H bond condensation reaction,which acts as a hydrogen or to balance the newly generated polyhedral borane charges,is proposed and studied in *** current study has led to a more effective and selective synthetic method for B12H122-and has also implicated the syntheses of other new polyhedral boranes.

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硇洲马尾藻的繁殖特性及体长生物量的季节变动

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水产学报 2011年第7期35卷 1015-1022页

作者：谢恩义贾柽陈秀丽杨彬广东海洋大学水产学院

广东特产的硇洲马尾藻是一种具有食用、药用价值的大型褐藻,有望开发成人工育苗栽培的新种类。2009年7月至2010年6月,原生态定点观察周年各月其体长和生物量等季节变动情况,结果发现,藻体假多年生,异株、异托。8—10月为体止期,11月至翌... 详细信息

广东特产的硇洲马尾藻是一种具有食用、药用价值的大型褐藻,有望开发成人工育苗栽培的新种类。2009年7月至2010年6月,原生态定点观察周年各月其体长和生物量等季节变动情况,结果发现,藻体假多年生,异株、异托。8—10月为体止期,11月至翌年4月中旬为生长期,4月中旬至6月初是繁殖期,6月初至7月底为衰老期。自然种群的繁衍和维持以假根再生为主,有性繁殖为辅。假根周年各月均可再生小苗,拔除假根,严重破坏种群繁衍,保留假根的剪收可以使第二年相同面积、同一时间的同种藻体的现存生物量明显增加。分布在水深2 m以内,宽5～10 m的潮间带岩礁上,11月开始加速生长,到5月上旬藻体长度生长达到最大值,原生态藻体的生物量表现出明显的季节变化,基本上与体长生长保持同步增长,最大生物量可达1 280 g/m2,最大单株藻体长1.73 m,最大单株质量可达250 g。

关键词：硇洲马尾藻繁殖习性生物量生长季节变动

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Catalytic atroposelective synthesis of indolyl quinazolinones bearing N-N/C-C diaxes

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Science China Chemistry 2025年

作者： Ning-yi Wang Shuo Gao Zhu-Di Shu Bei-Bei cheng Chun Ma Yu-chen Zhang Feng Shi Research Center of Chiral Functional Heterocycles School of Chemistry and Materials Science Jiangsu Normal University School of Petrochemical Engineering Changzhou University School of Chemistry and Chemical Engineering Henan Normal University

The first catalytic atroposelective synthesis of indole derivatives bearing N-N/C-C diaxes has been established by the strategy of de novo ring formation. By this strategy, various indolyl quinazolinones bearing vicinal N-N/C-C diaxes were synthesized in high yields with overall good diastereoselectivities and excellent enantioselectivities via chiral phosphoric acid-catalyzed onepot asymmetric formal (5+1) cycloaddition/oxidation reaction. Moreover, such indolyl quinazolinones bearing vicinal N-N/CC diaxes have good configurational stability and could be converted into new chiral ligands with applications in asymmetric catalysis. Therefore, this work not only offered a new member for the family of diaxial atropisomers, but also provided a powerful strategy for catalytic asymmetric synthesis of atropisomers bearing N-N/C-C diaxes.

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Comparative analysis of empirical and deep learning models for ionospheric sporadic E layer prediction

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Earth and Planetary Physics 2025年第1期9卷 10-19页

作者： BingKun Yu PengHao Tian XiangHui Xue Christopher JScott HaiLun Ye jianFei Wu Wen yi TingDi chen XianKang Dou Institute of Deep Space Sciences Deep Space Exploration LaboratoryHefei 230088China CAS Key Laboratory of Geospace Environment School of Earth and Space SciencesUniversity of Science and Technology of ChinaHefei 230026China Hefei National Laboratory University of Science and Technology of ChinaHefei 230088China Department of Meteorology University of ReadingReading RG66BBUK

Sporadic E(Es)layers in the ionosphere are characterized by intense plasma irregularities in the E region at altitudes of 90-130 *** they can significantly influence radio communications and navigation systems,accurate forecasting of Es layers is crucial for ensuring the precision and dependability of navigation satellite *** this study,we present Es predictions made by an empirical model and by a deep learning model,and analyze their differences comprehensively by comparing the model predictions to satellite RO measurements and ground-based ionosonde *** deep learning model exhibited significantly better performance,as indicated by its high coefficient of correlation(r=0.87)with RO observations and predictions,than did the empirical model(r=0.53).This study highlights the importance of integrating artificial intelligence technology into ionosphere modelling generally,and into predicting Es layer occurrences and characteristics,in particular.

关键词： ionospheric sporadic E layer radio occultation ionosondes numerical model deep learning model artificial intelligence

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基于大肠杆菌O157:H7快速检测技术的研究综述

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智慧健康 2021年第3期7卷 37-39页

作者：贾亦琛初浩展齐齐哈尔医学院医学技术学院黑龙江齐齐哈尔161000 青岛科技大学山东青岛266000

随着社会的发展,生活水平的提高,人们越来越注重生活的质量和身体健康,对事物中所携带的病菌也更加担忧,而大肠杆菌是人和许多动物肠道中最为主要且数目最多的一种细菌,且仍有少部分特殊类型的大肠杆菌具有相当强的毒性。人类一旦感染,... 详细信息

随着社会的发展,生活水平的提高,人们越来越注重生活的质量和身体健康,对事物中所携带的病菌也更加担忧,而大肠杆菌是人和许多动物肠道中最为主要且数目最多的一种细菌,且仍有少部分特殊类型的大肠杆菌具有相当强的毒性。人类一旦感染,将会造成不堪设想的疫情。于是怎么样能快速检测病菌,成为不可小觑的问题,也是本研究的重点内容。本文研究分析大肠杆菌O157:H7的致病性并综述基于微流控的快速检测技术的相关研究。

关键词：微生物 PCR 微生物检测微流控

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大型综合医院广域网平台的设计与实现

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中国数字医学 2007年第4期2卷 23-24页

作者：刘谦翁昌晶陈加宜海南省人民医院信息中心

结合海南省人民医院广域网络系统建设的实际工作,提出了一套大型综合医院计算机广域网平台的建设方案,作为对医院计算机广域网平台建设的初步探讨。

关键词：综合医院网络规划广域网

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