检索结果-南通市图书馆

The Study on the Stability Constant and Species Distribution of Eu (III) and Tb (III) Complexes with BDBPH

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2005年第12期16卷 1671-1674页

作者： Guo Qiang SHANGGUAN Jun ZHU ning WANG jia zuan ni Department of Chemistry Jining Medical CollegeJining 272013 Key Laboratory of Rare Earth-Chemistry and Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 Graduate School of Chinese Academy of Sciences Beijing 100039

The stability constants and species distributions of complexes of two lanthanide ions, Eu（Ⅲ） and Tb（Ⅲ）, with a macrocyclic ligand, BDBPH, in 1：1 and 2：lsystem, were determined potentiometrically at 25.0 ℃ and I=0.100 mol L^-l. The two metal ions could form deprotonated mono- or dinuclear complexes with BDBPH after the first two protons of the ligand completely neutralized. At higher pH values, Eu（Ⅲ） could not form hydroxy complexes with BDBPH, while Tb（Ⅲ） could form hydroxy complexes in the type of M2L（OH）, M2L（OH）2, and M2L（OH）3.

关键词： BDBPH Eu(Ⅲ) Tb(Ⅲ) stability constant species distribution

Expression and characterization of a new valosin-containing protein from silkworm

在线全文

学校读者我要写书评

暂无评论

Insect Science 2012年第5期19卷 549-558页

作者： Ping Chen Ruo-Ran Wang Zhen Peng Qiong Liu jia-zuan ni Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun College of Life Sciences Shenzhen University Shenzhen Guangdong Province Graduate University of the Chinese Academy of Sciences Chinese Academy of Sciences Changchun China

Valosin-containing protein （VCP） is a type-II adenosine triphosphatase （ATPase） wih extensive biological function in organisms. Silkworm is the second in- sect model for genetic studies and a bioreactor for proteinaceous drugs and biomaterials. In this paper, a new VCP-like gene was amplified from the fat body of silkworm follow- ing genome prediction and spliced expressed sequence tag sequences, using both reverse transcription polymerase chain reaction （RT-PCR） and 3＇-RACE （rapid amplification of complementary DNA ends） methods. Bioinformatical analysis showed that the translated amino acid sequence contained a highly conserved domain of VCPs similar to that of many insects. This domain consists of the conserved structure motifs of the ATP binding site and the catalytical center, which is closely related to the insect VCPs in a phylogenetic tree. The silkworm VCP-like gene was successfully inserted into the plasmid and transformed into Escherichia coli cells to express VCP-like protein with ATPase activity. The expression of silkworm VCP-like protein was also confirmed by Western blotting and mass spectromet- ric analyses. Distribution of the VCP-like gene in various tissues of the silkworm was also studied by real-time PCR. Results showed that the messenger RNA （mRNA） of VCP-like protein is widely expressed in fat body, reproductive organs （testis or ovary）, silk gland, head, Malpighian tubule, epidermis and midgut. Among them, fat body has the highest mRNA expression level of the VCP-like gene, while the midgut has the lowest expression level. This study provides groundwork for further study on the structure and function of the new VCP-like protein.

关键词： ATPase activity Bombyx mori gene cloning protein expression real-timPCR valosin-containing protein

Studies on the Effects of Epimedium Extract on Erythrocytes

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2006年第8期17卷 1105-1108页

作者： Xiao Yu ZHANG Feng Hua CHEN Ping Hui WEI jia zuan ni Department of Biological Engineering College of Environment ＆ Chemical Engineering Yanshan University Hebei 066004 Key Laboratory of Rare Earth Chemistry and Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022

The effect of Epimedium extract （EE） on erythrocytes was investigated by atomic force microscopy （AFM）. The images of the surface structures showed clear concave and progressive increase of surface roughness of erythrocyte after incubation with EE at concentration of 0.2 or 0.05 g/L, far below its critical hemolytic levels. The AFM results also indicated that the granules of the fine surface structure increased, which caused by aggregation of membrane protein. Further study showed that the change in surface topography of erythrocyte membrane might be connected with the increase of intracellular free Ca^2＋ induced by EE.

关键词： AFM Epimedium extract (EE) erythrocyte intracellular free Ca^2+.

NMR Studies on the Subacute Biochemical Effects of Aristolochic Acid on Rat Serum

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2005年第11期16卷 1507-1510页

作者： XiaoYu ZHANG Hui Feng WU Xiao Jing LI Feng Kui PEI jia zuan ni Key Laboratory of Rare Earth Chemistry and Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022

The subacute effect of aristolochic acid （AA） on rat serum was studied by NMR method. The biochemical effects induced by AA were characterized by an increase in the amounts of creatinine, trimethylamine N-oxide, acetoacetate, acetate and 3-D-hydroxybutyrate and lactate in serum from ^1H NMR spectra. Principal component analysis was used for further comparing the similarities of ^1H NMR spectral profiles of serum from rats treated with AA and model toxins.

关键词： Aristolochic acid NMR serum principal component analysis metabolite.

Study on Effect of Gd (III) Speciation on Ca (II) Speciationin Human Blood Plasma by Computer Simulation

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2001年第2期12卷 161-162页

作者： Yue WANG Xing LU Shu Yun WANG Jing Fen HAN Kui Yue YANG Chun Ji niU jia zuan ni Key Laboratory of Rare Earth Chemistry and Physics Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022

Ca (II) speciation and effect of Gd (III) speciation on Ca (II) speciation in human blood plasma were studied by computer simulation. [CaHCO3](-) is a predominant compound species of Ca (II). Gd (III) can compete with Ca (II) for biological molecules. The presence of Gd (III) results in a increase of concentration of free Ca (II) and a decrease of concentration of Ca (II) compounds.

关键词： Speciation blood plasma computer simulation calcium (II) gadolinium (III)

STUDY ON THE COORDINATION COMPOUNDS OF RARE EARTHS WITH DIPEPTIDE-COORDINATION OF RARE EARTHS WITH DL-ALANYL-ALAniNE

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1991年第8期2卷 669-672页

作者： jian Xue LIU Qing Bo MENG Chun Ji niU jia zuan ni Changchun Institute of Applied chemistry Acadimia SinicaChangchun 130022

In this paper protonation constants of the title lisand and stability constants of its coordination compounds with rare earths have been determined by potentiometric titration at 25±0.1℃ and ionic strength of ***^(-3)NaClO_4. The coordination of rare earths with DL-alanyl-alanine has been discussed. The stability of the coordination compound shows 'tetrad effect'. The position for yttrium shifts from its usual Bo-Er region to the vicinity of Tb.

关键词： RE STUDY ON THE COORDINATION COMPOUNDS OF RARE EARTHS WITH DIPEPTIDE-COORDINATION OF RARE EARTHS WITH DL-ALANYL-ALAniNE DL

Study on the Interaction of Rare Earth with 3, 5, 3'-Triiodothyronine

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1997年第2期8卷 181-182页

作者： Feng GAO Shu Yun WANG Shu Lan MENG Yan Min LIU Chun Ji niU jia zuan ni(Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Appliet Chemistry, Chinese Academy of Sciences, Changchun 130022) CHINESE ACAD SCI CHANGCHUN INST APPL CHEMLAB RARE EARTH CHEM & PHYSCHANGCHUN 130022PEOPLES R CHINA

The stability constants for rare earth complexes with 3, 5, 3'-triiodothyronine were determined at 37℃ and an ionic strength of 0.15 mole/L NaCl,The lanthanide induced shifts were measured for 1H nuclei of 3, 5, 3'-t... 详细信息

关键词： 110 Study on the Interaction of Rare Earth with 3

NMR AND INFRARED SPECTROSCOPIC STUDY ON THE INTERACTION OF RARE EARTH WITH L-METHIOniHE

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1992年第4期3卷 295-298页

作者： Chun Ji niU~* Feng GAO Qing Bo MENG jia zuan ni Changchun Institute of Applied chemistry, Academia Sinica, Changchun 130022

Five solid complexes of rare earth with L-methionine have been prepared. The interaction of rare earth with the ligand has been studied by NMR and IR spectroscopy.

关键词： NMR AND INFRARED SPECTROSCOPIC STUDY ON THE INTERACTION OF RARE EARTH WITH L-METHIOniHE CS

In-Situ Synthesis of Terbium Complex with Salicylic Acid in Silica Matrix by a Two-Step Sol-Gel Process

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 1998年第12期9卷 0-0,0-0页

作者： Lian She FU Hong Jie ZHANGl Shu Bin WANG Qing Guo MENG Km Yue YANG jia zuan ni(Laboratory of Rare Earth Chemistry and Physics, Changchun institute of Applied Chemistry,Chinese Academy of Sciences, Changchun, 130022) Chinese Acad Sci Changchun Inst Appl Chem Lab Rare Earth Chem & Phys Changchun 130022 Peoples R China

A process for in situ synthesis of terbium complex with salicylic acid by a two-step solgel method in silica matrix has been proposed. The luminescence properties of the silica gelscodoped with terbium and salicylic acid have also been discussed with respect to that of the geldoped with terbium and that of pure terbium complex with salicylic acid.

关键词： Sol-gel method In situ synthesis, rare earth complex