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A comparison of honeybee (Apis mellifera) queen, worker and drone larvae by RNA-Seq

Insect Science 2019年第3期26卷 499-509页

作者： Xu-jiang He Wu-Jun jiang Mi Zhou Andrew B. Barron zhi-jiang Zeng Honeybee Research Institute Jiangxi Agricultural University Nanchang China Biomarker Technologies Co. Ltd. Beijing China 2Department of Biological Sciences Macquarie University North Ryde NSW Australia

Honeybees (Apis mellifera) have haplodiploid sex determination: males develop from unfertilized eggs and females develop from fertilized ones. The differences in larval food also determine the development of females. Here we compared the total somatic gene expression profiles of 2-day and 4-day-old drone, queen and worker larvae by RNASeq. The results from a co-expression network analysis on all expressed genes showed that 2-day-old drone and worker larvae were closer in gene expression profiles than 2-day-old queen larvae. This indicated that for young larvae (2-day-old) environmental factors such as larval diet have a greater effect on gene expression profiles than ploidy or sex determination. Drones had the most distinct gene expression profiles at the 4-day larval stage, suggesting that haploidy, or sex dramatically affects the gene expression of honeybee larvae. Drone larvae showed fewer differences in gene expression profiles at the 2-day and 4-day time points than the worker and queen larval comparisons (598 against 1190 and 1181), suggesting a different pattern of gene expression regulation during the larval development of haploid males compared to diploid females. This study indicates that early in development the queen caste has the most distinct gene expression profile, perhaps reflecting the very rapid growth and morphological specialization of this caste compared to workers and drones. Later in development the haploid male drones have the most distinct gene expression profile, perhaps reflecting the influence of ploidy or sex determination on gene expression.

关键词： caste differentiation envir on mental factors gene expression haploid and diploid honeybees larval development

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Modeling impact of culture facilities on hydrodynamics and solute transport in marine aquaculture waters of North Yellow Sea

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Water Science and Engineering 2023年第1期16卷 26-35页

作者： Heng-zhi jiang Yong-peng Ji Ming-liang Zhang National Marine Environmental Monitoring Center Dalian 116023China Beijing Jiutian Weather Co. LtdBeijing 100081China School of Ocean Science and Environment Dalian Ocean UniversityDalian 116023China

An increasing number of marine aquaculture facilities have been placed in shallow bays and open sea,which might significantly affect hydrodynamic and solute transport processes in marine aquaculture *** this study,a coupled hydrodynamic and solute transport model was developed with high-resolution schemes in marine aquaculture waters based on depth-averaged shallow water equations.A new expression of drag force was incorporated into the momentum equations to express the resistance of suspended culture *** coupled model was used to simulate the effect of suspended structures on tidal currents and the movement of a contaminant cloud in the marine aquaculture of the North Yellow Sea,*** simulation results showed a low-velocity area appearing inside the aquaculture cage area,with a maximum reduction rate of velocity close to 45%under high-density *** results also showed that tidal currents were sensitive to the density of suspended cages,the length of cages,and the drag coefficients of *** transport processes of pollutants inside aquaculture facilities were inhibited away from the vicinity of the culture cage area because of the diminished tidal ***,the suspended cages significantly affected the transport processes of pollutants in the coastal aquaculture ***,the reduced horizontal velocity significantly decreased the food supply for the aquaculture areas from the surrounding sea.

关键词： Marine aquaculture Suspended cages Numerical simulation Current reduction Solute transport

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Advances in treatment of neurodegenerative diseases: Perspectives for combination of stem cells with neurotrophic factors

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World Journal of Stem Cells 2020年第5期12卷 323-338页

作者： Jie Wang Wei-Wei Hu zhi jiang Mei-jiang Feng Department of Geriatrics the Second Affiliated Hospital of Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina Department of Neurology the Affiliated Jiangning Hospital of Nanjing Medical UniversityNanjing 211100Jiangsu ProvinceChina Department of Geriatrics Jinling HospitalMedical School of Nanjing UniversityNanjing 210002Jiangsu ProvinceChina Key Laboratory for Aging&Disease Nanjing Medical UniversityNanjing 210011Jiangsu ProvinceChina

Neurodegenerative diseases,including Alzheimer’s disease,Parkinson’s disease,Huntington’s disease and amyotrophic lateral sclerosis,are a group of incurable neurological disorders,characterized by the chronic progressive loss of different neuronal ***,despite its increasing prevalence among the everincreasing aging population,little progress has been made in the coincident immense efforts towards development of therapeutic *** interest has recently turned towards stem cells including stem cells-derived exosomes,neurotrophic factors,and their combination as potential therapeutic agents in neurodegenerative *** this review,we summarize the progress in therapeutic strategies based on stem cells combined with neurotrophic factors and mesenchymal stem cells-derived exosomes for neurodegenerative diseases,with an emphasis on the combination therapy.

关键词： Neurodegenerative diseases Stem cells Brain-derived neurotrophic factor Glial cell line-derived neurotrophic factor Nerve growth factor Combination therapy

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Autophagy plays a protective role in advanced glycation end products-induced apoptosis of chondrocytes via regulation of tumor necrosis factor-α,nuclear factor-κ B and reactive oxygen species

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Asian Pacific Journal of Tropical Medicine 2018年第1期11卷 73-77页

作者： zhi-jiang Sun Ya-Yi Xia Orthopaedics Key Laboratory of Gansu Province The Second Hospital of Lanzhou University

Objective: To study the adverse effects of advanced glycation end products(AGEs) on chondrocytes and the role of autophagy in this process. Methods: Chondrocytes were harvested from the human articular cartilage tissues in surgery. AGEs were administered during chondrocytes culture. The rapamycin was used to induce autophagy. The cell viability was determined by 3-[4,5-dimethylthiazol2-yl]-2,5-diphenyl tetrazolium bromide(MTT) *** expression of tumor necrosis factor-α(TNF-α) and nuclear factor-κ B(NF-κ B) was detected by quantitative real-time polymerase chain reaction. The reactive oxygen species(ROS) production and apoptosis of the chondrocytes were determined by fluorescent probe and flow cytometer, respectively. Results: The chondrocytes viability was significantly reduced after 12 h incubation with AGEs(P<0.01)). In contrast, rapamycin pretreatment increased the chondrocytes viability through autophagy. AGEs increased TNF-α and NF-κ B mRNA expression of chondrocytes and autophagy receded or proceeded the change. AGEs increased intracellular ROS accumulation and autophagy reversed the change. AGEs accelerated chondrocytes apoptosis and autophagy suspended apoptosis. Conclusions: Accumulation of AGEs may have an adverse role for chondrocytes by increasing TNF-α and NF-κB expression, ROS accumulation and apoptosis; meanwhile, autophagy ameliorates the AGEsinduced adverse effects.

关键词： Advanced glycation end products Autophagy Tumor necrosis factor-α Nuclear factor-κ B Reactive oxygen species Apoptosis Chondrocytes

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Salt-induced Electron Transfer Photooxidation of Cholesterol in Nonpolar Solvents

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Chemical Research in Chinese Universities 1997年第4期13卷 112-116页

作者： YANG Chen gen WAN Yan jiang zhi qin Department of Organic Chemistry Agricultural CollegeYangzhou UniversityYangzhou225009 Analysis and Research Center Tongji UniversityShanghai200092

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Ｓａｌｔ┐ｉｎｄｕｃｅｄＥｌｅｃｔｒｏｎＴｒａｎｓｆｅｒＰｈｏｔｏｏｘｉｄａｔｉｏｎｏｆＣｈｏｌｅｓｔｅｒｏｌｉｎＮｏｎｐｏｌａｒＳｏｌｖｅｎｔｓＹＡＮＧＣｈｅｎ－ｇｅｎ＊（ＤｅｐａｒｔｍｅｎｔｏｆＯｒｇａｎｉｃＣｈｅｍｉｓｔｒｙ，Ａｇｒｉｃｕｌｔｕ...

关键词： Cholesterol Photooxygenation Salt induced Electron transfer

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Catalytic activity of porous manganese oxides for benzene oxidation improved via citric acid solution combustion synthesis

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Journal of Environmental Sciences 2020年第12期32卷 196-204页

作者： Hao Guo zhixiang Zhang zhi jiang Mingxia Chen Hisahiro Einaga Wenfeng Shangguan Research Center for Combustion and Environmental Technology Shanghai Jiao Tong UniversityShanghai 200240China Department of Energy and Material Sciences Faculty of Engineering SciencesKyushu UniversityFukuoka 816-8580Japan

Various manganese oxides(MnOx) prepared via citric acid solution combustion synthesis were applied for catalytic oxidation of benzene. The results showed the ratios of citric acid/manganese nitrate in synthesizing process positively affected the physicochemical properties of MnOx, e.g., BET(Brunauer-Emmett-Teller) surface area, porous structure, reducibility and so on, which were in close relationship with their catalytic performance. Of all the catalysts, the sample prepared at a citric acid/manganese nitrate ratio of 2:1(C2M1) displayed the best catalytic activity with T(90)(the temperature when 90% of benzene was catalytically oxidized) of 212 ℃. Further investigation showed that C2M1 was Mn2O3 with abundant nano-pores, the largest surface area and the proper ratio of surface Mn^4+/Mn^3+, resulting in preferable low-temperature reducibility and abundant surface active adsorbed oxygen species. The analysis results of the in-situ Fourier transform infrared spectroscopy(in-situ FTIR) revealed that the benzene was successively oxidized to phenolate, o-benzoquinone, small molecules(such as maleates, acetates, and vinyl), and finally transformed to CO2 and H2O.

关键词： Catalytic activity Benzene oxidation Porous manganese oxides Citric acid Solution combustion

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Catalytic degradation of benzene over non-thermal plasma coupled Co-Ni binary metal oxide nanosheet catalysts

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Journal of Environmental Sciences 2023年第10期132卷 1-11页

作者： zhi jiang Dongxu Fang Yuting Liang Yaoyu He Hisahiro Einaga Wenfeng Shangguan Research Center for Combustion and Environmental Technology Shanghai Jiao Tong UniversityShanghai 200240China Department of Energy and Material Sciences Faculty of Engineering SciencesKyushu University6-1 Kasuga KoenKasugaFukuoka 816-8580Japan

Non-thermal plasma(NTP)has been demonstrated as one of the promising technologies that can degrade volatile organic compounds(VOCs)under ambient ***,one of the key challenges of VOCs degradation in NTP is its relatively low mineralization rate,which needs to be addressed by introducing ***,the design and optimization of catalysts have become the focus of NTP coupling catalysis *** thiswork,a series of two-dimensional nanosheet Co-Ni metal oxides were synthesized by microwave method and investigated for the catalytic oxidation of benzene in an NTP-catalysis coupling *** them,Co_(2)Ni_(1)O_(x)achieves 60%carbon dioxide(CO_(2))selectivity(SCO_(2))when the benzene removal efficiency(REbenzene)reaches more than 99%,which is a significant enhancement compared with the CO_(2)selectivity obtained without any catalysts(38%)under the same input *** intriguingly,this SCO_(2)is also significantly higher than that of single metal oxides,NiO or Co_(3)O_(4),which is only around 40%.Such improved performance of this binary metal oxide catalyst is uniquely attributed to the synergistic effects of Co and Ni in Co_(2)Ni_(1)O_(x)*** introduction of Co_(2)Ni_(1)O_(x)was found to promote the generation of acrolein significantly,one of the key intermediates found in NTP alone system reported previously,suggest the benzene ring open reaction is *** with monometallic oxides NiO and Co_(3)O_(4),Co_(2)Ni_(1)O_(x)also shows higher active oxygen proportion,better oxygenmobility,and stronger low-temperature redox *** above factors result in the improved catalytic performance of Co_(2)Ni_(1)O_(x)in the NTP coupling removal of benzene.

关键词： Benzene Nonthermal plasma Catalysis Cobalt metal oxide Nickel oxide Binary metal oxide

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In situ revealing the reconstruction behavior of monolayer rocksalt CoO nanosheet as water oxidation catalyst

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Journal of Energy Chemistry 2022年第7期31卷 373-381,I0010页

作者： Weiqi Guo Haolin Luo Dongxu Fang zhi jiang Jiasheng Chi Wenfeng Shangguan Research Center for Combustion and Environment Technology Shanghai Jiao Tong UniversityShanghai 200240China Center of Hydrogen Science Shanghai Jiao Tong UniversityShanghai 200240China

The reconstruction during oxygen evolution reaction (OER) significantly affects the electronic and local geometry structure of metal sites in *** with well-investigated cobalt-based materials,the reconstruction of rocksalt CoO with purely Co^(2+) in octahedral (Oh) coordination has not been revealed in ***,monolayer Co O supported on reduced graphene oxide (r GO) was synthesized via a one-pot hydrothermal strategy with calcinating in Ar *** structure evolution of twodimension (2D) Co O/r GO during OER was revealed by in situ X-ray absorption spectroscopy (XAS).The transition from Co O toward Co3O_(4) already occurred at open circuit potential,further enhanced at 1.23 V (***).The Co Ox(OH)ywas determined as the active phase at 1.53 V,displaying a tetrahedral Co coordination defective spinel Co_(3)O_(4) with the Co-O shell that featured the (oxy)hydroxide,not the standard Co *** OER,the irreversible transition from CoO to Co_(3)O_(4) was *** contrast,in situ Raman spectra revealed a reversible amorphization process on Co_(3)O_(4)/r GO under operation ***,this study indicated that the reconstruction behavior could be more effectively revealed by XAS using 2D materials.

关键词： Oxygen evolution reaction Two-dimension materials Rocksalt CoO X-ray absorption spectroscopy In situ experiments

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csd alleles in the red dwarf honey bee （Apis florea, Hymenoptera： Apidae） show exceptionally high nucleotide diversity

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Insect Science 2011年第6期18卷 645-651页

作者： zhi-Yong Liu Zi-Long Wang Xiao-Bo Wu Wei-Yu Yan zhi-jiang Zeng Honeybee Research Institute Jiangxi Agricultural University Experimental Animal Center Institute of Occupational Disease Prevention Nanchang China

The single locus complementary sex determination （sl-csd） gene is the primary gene determining the gender of honey bees （Apis spp.）. While the csd gene has been well studied in the Western honey bee （Apis mellifera）, and comparable data exist in both the Eastern honey bee （Apis cerana） and the giant honey bee （Apis dorsata）, no studies have been conducted in the red dwarf honey bee, Apisflorea. In this study we cloned the genomic region 3 of the A. florea csd gene from 60 workers, and identified 12 csd alleles. Analysis showed that similar to A. mellifera, region 3 of the csd gene contains a RS domain at the N terminal, a proline-rich domain at the C terminal, and a hypervariable region in the middle. However, the A. florea csd gene possessed a much higher level of nucleotide diversity, compared to A. mellifera, A. cerana and Apis dorsata. We also show that similar to the other three Apis species, in A. florea, nonsynonymous mutations in the csd gene are selectively favored in young alleles.

关键词： balancing selection complementary sex determination csd allele nucleotidediversity polymorphism positive selection

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Preparation and optical properties of composite thin films with embedded InP nanoparticles

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Chinese Science Bulletin 2001年第6期46卷 461-466页

作者： Maojun Zheng Lide Zhang Guanghai Li zhi jiang Chinese Acad Sci Inst Solid State Phys Hefei 230031 Peoples R China

InP nanoparticles embedded in SiO2 thin films were prepared by radio-frequency magnetron co-sputtering. We analyzed the structure and growth behavior of the composite films under different preparation conditions. X-ray diffraction and Raman spectroscopy analyses indicate that InP nanoparticles have a polycrystalline structure. The average size of InP nanoparticles is in the range of 3-10 nm. The broadening and red shift of the Raman peaks were observed, which can be interpreted by the phonon confinement model. Optical transmission spectra indicate that the opticalabsorption edges of the films can be modulated in the visible light range. The marked blue shift of the absorption edge with respect to that of bulk InP is explained by the quantum confinement effect. The theoretical values of the blue shift predicted by the effective mass approximation model are different from the experimental results for the InP-SiO2 system. Analyses indicate that the exciton effective mass of the InP nanoparticles is not

关键词： nanocomposite thin film sputtering phonon confine- ment effect quantum confinement effect.

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