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Capsosiphon fulvescens suppresses LPS-stimulated inflammatory responses by suppressing TLR4/NF-κB activation in RAW264.7 murine macrophages

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Asian Pacific Journal of Tropical Biomedicine 2024年第3期14卷 115-126页

作者： Seon Yeong ji Eunjin Bang Hyun Hwangbo Min Yeong Kim Da Hye Kim su Hyun Hong Shin-Hyung Park Chang-Young Kwon Gi-Young Kim You-jin Jeon suengmok Cho Yung Hyun Choi Department of Biochemistry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Basic Research Laboratory for the Regulation of Microplastic-Mediated Diseases and Anti-Aging Research Center Dong-eui UniversityBusan 47227Republic of Korea Department of Pathology Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Oriental Neuropsychiatry Dong-eui University College of Korean MedicineBusan 47227Republic of Korea Department of Marine Life Science Jeju National UniversityJeju 63243Republic of Korea Department of Food Science and Technology/Institute of Food Science Pukyong National UniversityBusan 48513Republic of Korea

Objective:To evaluate the effects of Capsosiphon fulvescens(***)ethanolic extract on inflammation in lipopolysaccharide(LPS)-induced RAW296.7 ***:The protective effects of *** ethanolic extract on LPS-induced inflammation in RAW264.7 macrophages were assessed using biochemical analysis,including enzyme-linked immunosorbent assay,quantitative reverse transcription-polymerase chain reaction,and Western blot *** examine reactive oxygen species(ROS)production,flow cytometry analysis,and immunofluorescence staining were ***,the modulatory effect of *** ethanolic extract on NF-κB activation was ***:*** ethanolic extract significantly attenuated LPS-induced levels of pro-inflammatory cytokines and notably reduced the secretion and mRNA levels of LPS-mediated matrix *** addition,*** ethanolic extract decreased ROS production and suppressed the TLR4/NF-κB signaling ***:*** ethanolic extract alleviates inflammation as well as oxidative stress by modulating the TLR4/NF-κB signaling in LPS-induced RAW264.7 *** can be used as a potential therapeutic agent to suppress inflammation and oxidative stress-associated diseases.

关键词： Capsosiphon fulvescens Inflammation Oxidative stress NF-κB Nrf2 TLR4

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表达鸡RSAD2蛋白的重组杆状病毒的构建与鉴定

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中国家禽 2024年第1期46卷 62-69页

作者：张婕柴家前苏帅山东农业大学动物科技学院(动物医学院) 山东省动物生物工程与疾病防治重点实验室山东泰安271018

为研究鸡S-腺苷甲硫氨酸基区域蛋白2(RSAD2)生物学功能,试验构建原核表达重组质粒pGEX-RSAD2,采用SDS-PAGE、Western blot验证RSAD2融合蛋白的表达,将融合蛋白免疫小鼠制备多克隆抗体。扩增RSAD2基因克隆至pFsatBac dual真核表达载体,... 详细信息

为研究鸡S-腺苷甲硫氨酸基区域蛋白2(RSAD2)生物学功能,试验构建原核表达重组质粒pGEX-RSAD2,采用SDS-PAGE、Western blot验证RSAD2融合蛋白的表达,将融合蛋白免疫小鼠制备多克隆抗体。扩增RSAD2基因克隆至pFsatBac dual真核表达载体,重组质粒经转座、蓝白斑筛选后,获得重组杆状病毒质粒pFastBac-RSAD2,重组杆粒转染至sf9昆虫细胞后收获重组杆状病毒rBac-RSAD2。用鸡传染性贫血病毒(CIAV)感染孵育RSAD2蛋白的MDCC-MSB1细胞,通过荧光定量PCR检测病毒在细胞中的增殖情况。结果显示:成功构建原核表达质粒pGEX-RSAD2,诱导表达了69 ku的RSAD2融合蛋白,制备的多克隆抗体能够与表达RSAD2蛋白的DF-1细胞特异性结合;重组杆状病毒rBac-RSAD2能够引起sf9细胞病变,且感染后的sf9细胞与制备的RSAD2多克隆抗体能够特异性结合,rBac-RSAD2表达了43 ku的RSAD2蛋白;相较对照组,孵育蛋白后的MDCC-MSB1细胞中的病毒载量降低。研究表明,成功构建了表达鸡RSAD2蛋白的重组杆状病毒rBac-RSAD2,其表达的RSAD2蛋白能够抑制CIAV增殖。

关键词：鸡 RSAD2 多克隆抗体杆状病毒真核表达

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教育领域下多维度特征命名实体识别方法

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计算机工程 2024年第10期50卷 110-118页

作者：任义苏博袁帅沈阳建筑大学计算机科学与工程学院辽宁沈阳110168

信息技术的发展与进步促使“互联网+教育”成为目前教育领域的研究热点,教育教学的各个环节都在向智能化的方向发展。中学数学的命名实体识别(NER)任务的研究,可为后续构建中学数学学科知识图谱及自动问答等任务奠定基础,进而满足中学... 详细信息

信息技术的发展与进步促使“互联网+教育”成为目前教育领域的研究热点,教育教学的各个环节都在向智能化的方向发展。中学数学的命名实体识别(NER)任务的研究,可为后续构建中学数学学科知识图谱及自动问答等任务奠定基础,进而满足中学生个性化知识获取的需求,助力新型智能化教育体系的构建。目前中学数学知识语义复杂,其NER和研究数据较少,且在当前主流模型特征提取任务中容易忽略掉部分局部特征。为解决该领域的实体识别困难问题,以自建的中学数学知识语料库为研究对象,提出一种融合多头注意力的多维度特征NER方法。该方法首先采用BERT进行文本表征预训练得到词向量,接着引入对抗训练对每个嵌入向量进行扰动,将得到的对抗样本和嵌入向量传送到多维度特征提取层进行特征提取,再将输出的特征进行拼接,通过多头注意力机制进行动态融合,最终经过条件随机场(CRF)修正后输出。实验结果表明,该方法在自建Educ数据集上的识别准确率、召回率以及F1值分别达到96.68%、97.71%和97.19%,证明了该方法在中学数学知识实体识别上的有效性。

关键词：命名实体识别教育领域对抗训练多维度特征提取多头注意力机制

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Highly Thermally Conductive and Structurally Ultra‑Stable Graphitic Films with Seamless Heterointerfaces for Extreme Thermal Management

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Nano-Micro Letters 2024年第3期16卷 383-397页

作者： Peijuan Zhang Yuanyuan Hao Hang Shi jiahao Lu Yingjun Liu Xin Ming Ya Wang Wenzhang Fang Yuxing Xia Yance Chen Peng Li Ziqiu Wang Qingyun su Weidong Lv ji Zhou Ying Zhang Haiwen Lai Weiwei Gao Zhen Xu Chao Gao MOE Key Laboratory of Macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringKey Laboratory of Adsorption and Separation Materials and Technologies of Zhejiang ProvinceZhejiang University38 Zheda RoadHangzhou 310027People’s Republic of China Shanxi-Zheda Institute of Advanced Materials and Chemical Engineering Taiyuan 030032People’s Republic of China Beijing Spacecrafts Manufacturing Co. LtdBeijing Friendship Road 104Haidian DistrictBeijing 100094People’s Republic of China Beijing Institute of Space Mechanics and Electricity Beijing Friendship Road 104Haidian DistrictBeijing 100094People’s Republic of China China Academy of Aerospace Aerodynamics Beijing 100074People’s Republic of China Hangzhou Gaoxi Technol Co. LtdHangzhou 311113People’s Republic of China International Research Center for X Polymers International CampusZhejiang UniversityHaining 314400People’s Republic of China

Highly thermally conductive graphitic film(GF)materials have become a competitive solution for the thermal management of high-power electronic ***,their catastrophic structural failure under extreme alternating thermal/cold shock poses a significant challenge to reliability and ***,we present the first investigation into the structural failure mechanism of GF during cyclic liquid nitrogen shocks(LNS),which reveals a bubbling process characterized by“permeation-diffusion-deformation”*** overcome this long-standing structural weakness,a novel metal-nanoarmor strategy is proposed to construct a Cu-modified graphitic film(GF@Cu)with seamless *** well-designed interface ensures superior structural stability for GF@Cu after hundreds of LNS cycles from 77 to 300 ***,GF@Cu maintains high thermal conductivity up to 1088 W m^(−1)K^(−1)with degradation of less than 5%even after 150 LNS cycles,superior to that of pure GF(50%degradation).Our work not only offers an opportunity to improve the robustness of graphitic films by the rational structural design but also facilitates the applications of thermally conductive carbon-based materials for future extreme thermal management in complex aerospace electronics.

关键词： Highly thermally conductive Structurally ultra-stable Graphitic film Extreme thermal management Liquid nitrogen bubbling

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考虑双重信息不对称及互动二元性的医患信任机制

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同济大学学报（自然科学版） 2024年第11期52卷 1803-1812页

作者：苏强季荔同济大学经济与管理学院上海200092

从医患双方出发,考虑信任关系建立中医患互动的二元性,构建双重信息不对称下逆向选择与道德风险同时存在的医患信任演化博弈模型。通过模型求解和仿真模拟,分析各因素对医患信任的影响。结果表明,医患双方对彼此的期望高于实际能力并不... 详细信息

从医患双方出发,考虑信任关系建立中医患互动的二元性,构建双重信息不对称下逆向选择与道德风险同时存在的医患信任演化博弈模型。通过模型求解和仿真模拟,分析各因素对医患信任的影响。结果表明,医患双方对彼此的期望高于实际能力并不总是导致负面情绪,在期望高于实际但不超过临界值的情况下,合理的高期望有助于双方信任策略演化;在确保患方信息受益的情况下,即使相对医方存在信息劣势,医患双方依旧倾向于信任策略;提高医方沟通能力、降低患者道德风险对信任策略选择更为关键。最后,结合1·20北京朝阳医院伤医案事件,讨论了医患沟通、期望及道德风险等因素对医患双方信任行为的演化结果,论证了模型的有效性。

关键词：医患信任双重信息不对称互动二元性演化博弈

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面向动态场景去模糊的对偶学习生成对抗网络

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控制与决策 2024年第4期39卷 1305-1314页

作者：纪野戴亚平廣田薰邵帅北京理工大学自动化学院北京100081 北京理工大学复杂系统智能控制与决策国家重点实验室北京100081

针对动态场景下的图像去模糊问题,提出一种对偶学习生成对抗网络(dual learning generative adversarial network,DLGAN),该网络可以在对偶学习的训练模式下使用非成对的模糊图像和清晰图像进行图像去模糊计算,不再要求训练图像集合必... 详细信息

针对动态场景下的图像去模糊问题,提出一种对偶学习生成对抗网络(dual learning generative adversarial network,DLGAN),该网络可以在对偶学习的训练模式下使用非成对的模糊图像和清晰图像进行图像去模糊计算,不再要求训练图像集合必须由模糊图像与其对应的清晰图像成对组合而成.DLGAN利用去模糊任务与重模糊任务之间的对偶性建立反馈信号,并使用这个信号约束去模糊任务和重模糊任务从两个不同的方向互相学习和更新,直到收敛.实验结果表明,在结构相似度和可视化评估方面,DLGAN与9种使用成对数据集训练的图像去模糊方法相比具有更好的性能.

关键词：动态场景去模糊对偶学习生成对抗网络注意力引导特征图损耗函数

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叙事结构理论视角下数据故事模型构建研究

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现代情报 2024年第7期44卷 13-21页

作者：宿倩纪雪梅山东理工大学信息管理学院山东淄博255049

[目的/意义]数据故事是数据与故事的结合,具有叙事性和结构性,从叙事结构理论视角可以厘清数据故事的结构要素,进而构建数据故事模型,指导数据故事创作。[方法/过程]首先,基于叙事结构理论从故事点、叙事意图、叙事逻辑、视觉效果4个层... 详细信息

[目的/意义]数据故事是数据与故事的结合,具有叙事性和结构性,从叙事结构理论视角可以厘清数据故事的结构要素,进而构建数据故事模型,指导数据故事创作。[方法/过程]首先,基于叙事结构理论从故事点、叙事意图、叙事逻辑、视觉效果4个层次剖析数据故事结构,得到数据故事模型框架;其次,从原料层、目标层、结构层和呈现层4个层次构建数据故事模型,对数据故事构建的生命周期进行阐释;最后,通过制作山东省农业碳排放数据故事对构建的数据故事模型进行实例分析。[结果/结论]该模型能够指导数据故事的生成和结构要素的呈现,对于提升数据的可读性、可理解性和逻辑性具有一定的理论与实践意义。

关键词：叙事结构叙事要素数据故事数据叙事山东省农业碳排放

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Understanding synergistic catalysis on Pt–Cu diatomic sites via operando X-ray absorption spectroscopy in sulfur redox reactions

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eScience 2024年第5期4卷 110-119页

作者： shuai Xie Xingjia Chen Leilei Wang Guikai Zhang Haifeng Lv Guolei Cai Ying-Rui Lu Ting-Shan Chan e jing Zhang Juncai Dong Hongchang jin Xianghua Kong Junling Lu Song jin Xiaojun Wu Hengxing ji Hefei National Laboratory for Physical Sciences at the Microscale CAS Key Laboratory of Materials for Energy ConversionDepartment of Applied ChemistryUniversity of Science and Technology of ChinaHefei 230026China School of Chemistry and Material Sciences CAS Key Laboratory of Materials for Energy Conversionand CAS Center for Excellence in Nanoscience and Synergetic Innovation of Quantum Information and Quantum TechnologyUniversity of Science and Technology of ChinaHefei 230026China Hefei National Laboratory for Physical Sciences at the Microscale Department of Chemical PhysicsSchool of Chemistry and Materials ScienceUniversity of Science and Technology of ChinaHefei 230026China Beijing Synchrotron Radiation Laboratory Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China National Synchrotron Radiation Research Center Hsinchu 300092China School of Chemistry and Chemical Engineering Hefei University of TechnologyHefei 230009China

sulfur redox reactions render lithium–sulfur(Li–S)batteries with an energy density of>500Whkg−1 but suffer a low practical capacity and fast capacity fade due to sluggish sulfur redox reaction(SRR)kinetics,which lies in the complex reaction process that involves a series of reaction intermediates and proceeds via a cascade ***,we present a Pt–Cu dual-atom catalyst(Pt/Cu-NG)as an electrocatalyst for sulfur redox ***/Cu-NG enabled the rapid conversion of soluble polysulfide intermediates into insoluble Li2S2/Li2S,and consequently,it prevented the accumulation and shuttling of lithium polysulfides,thus outperforming the corresponding single-atom catalysts(SACs)with individual Pt or Cu *** X-ray absorption spectroscopy and density functional theory calculations revealed that a synergistic effect between the paired Pt and Cu atoms modifies the electronic structure of the Pt site through d-orbital interactions,resulting in an optimal moderate interaction of the metal atom with the different sulfide *** optimal interaction enhanced charge transfer kinetics and promoted sulfur redox *** work thus provides important insights on the atomic scale into the synergistic effects operative in dual-atom catalysts and will thus pave the way to electrocatalysts with enhanced efficiency for high-performance Li–S batteries.

关键词： Dual-atom catalysts sulfur redox reaction Lithium-sulfur batteries Synergistic interaction X-ray absorption spectroscopy

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Genomic surveillance for SARS-CoV-2 Variants: Dominance of XBB Replacement — China, January–June 2023

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China CDC weekly 2024年第15期6卷 324-331页

作者： Yenan Feng Qiudong su Lili Li Xiaozhou He Peihua Niu Xiaojuan Guo Xiang Zhao jing Tang Zhiyuan jia ji Wang Changcheng Wu Baicheng Xia Zhixiao Chen Yuchao Wu jing Yang Songqi Chen Cao Chen Shiwen Wang Xiaoping Dong National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina NHC Key Laboratory of Medical Virology and Viral Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina

Introduction:In the first half of 2023,a global shift was observed towards the predominance of XBB *** faced a significant epidemic between late 2022 and early 2023 due to Omicron subvariants BA.5.2 and *** study aims to depict the evolving variant distribution among provincial-level administrative divisions(PLADs)in China and explore the factors driving the predominance of XBB ***:Sequences from local and imported coronavirus disease 2019(COVID-19)cases recorded between January 1 and June 30,2023,were *** study analyzed the changing distribution of viral variants and assessed how the prior dominance of specific variants,XBB subvariants,and imported cases influenced the prevalence of the XBB replacement ***:A total of 56,486 sequences were obtained from local cases,and 8,669 sequences were from imported *** in April,there was a shift in the prevalence of XBB from imported to local cases,with varying dominance among *** PLADs previously high in BF.7,the rise of XBB was delayed.A positive correlation was found between XBB proportions in imported cases from January to March and local cases in *** distribution pattern of XBB subvariants differed between local and imported cases within the same *** significant differences were noted in the replacement rates of XBB ***:The timing of XBB dominance differed among various PLADs in China in the first half of 2023,correlating closely with the prevalence of XBB variants among imported cases.

关键词： dominance imported distribution

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Variable phenotypes and outcomes associated with the MMACHC c.482G>A mutation:follow-up in a large CblC disease cohort

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World Journal of Pediatrics 2024年第8期20卷 848-858页

作者： Sheng-Nan Wu Hui-Shu E Yue Yu Shi-Ying Ling Li-Li Liang Wen-Juan Qiu Hui-Wen Zhang Rui-Xue shuai Hai-Yan Wei Chi-Ju Yang Peng Xu Xi-Gui Chen Hui Zou ji-Zhen Feng Ting-Ting Niu Hai-Li Hu Kai-Chuang Zhang De-Yun Lu Zhu-Wen Gong Xia Zhan Wen-Jun ji Xue-Fan Gu Yong-Xing Chen Lian-Shu Han Department of Endocrinology and Metabolism Henan Key Laboratory of Children’s Genetics and Metabolic DiseasesChildren’s Hospital Affiliated to Zhengzhou UniversityHenan Children’s HospitalZhengzhou Children’s HospitalNo.255 Gangdu StreetZhengzhouChina Department of Pediatric Endocrinology and Genetics Fujian Children’s HospitalFuzhouChina The Center for Pediatric Liver Diseases Children’s HospitalFudan UniversityShanghaiChina Department of Pediatric Endocrinology/Genetics Shanghai Institute for Pediatric ResearchXinhua HospitalSchool of MedicineShanghai Jiao Tong UniversityNo.1665 Kongjiang RoadShanghaiChina Department of Pediatrics Second Affiliated Hospital of Naval Medical UniversityShanghaiChina Center of Neonatal Disease Screening Jining Maternal and Child Health Care HospitalJiningChina Center of Neonatal Disease Screening Jinan Maternal and Child Health Care HospitalJinanChina Center of Neonatal Disease Screening Shijiazhuang Maternal and Child Health Care HospitalShijiazhuangChina Center of Neonatal Disease Screening Shandong Maternal and Child Health Care HospitalJinanChina Center of Neonatal Disease Screening Hefei Maternal and Child Health Care HospitalHefeiChina

Background The aim of this study was to characterize the variable phenotypes and outcomes associated with the methylmalonic aciduria and homocystinuria type C protein gene(MMACHC)c.482G>A mutation in 195 Chinese cases with CblC *** We carried out a national,retrospective multicenter study of 195 Chinese patients with CblC disease attributable to the MMACHC c.482G>A variant either in a homozygous or compound heterozygous *** control group consisted of 200 patients diagnosed with CblC disease who did not possess the c.482G>A *** features,including disease onset,symptoms,biochemical metabolites,gene mutation,and follow-up outcomes were reviewed and analyzed in *** median follow-up period spanned 3 years and 8 months,with a range of 1 year and 2 months to 12 years and 10 *** Among 195 patients carrying the c.482G>A variant,125(64.1%)cases were diagnosed by newborn screening(NBS),60(30.8%)cases were detected due to disease onset,and 10(5.1%)cases were identified from sibling *** hundred and seventeen(93.6%)individuals who were diagnosed by NBS,and nine patients who came from sibling diagnoses remained asymptomatic in this *** 69 symptomatic patients of the c.482G>A group,more patients presented with later onset,and the top six common clinical symptoms at disease onset were developmental delay(59.4%),lower limb weakness and poor exercise tolerance(50.7%),cognitive decline(37.7%),gait instability and abnormal posture(36.2%),seizures(26.1%),and psychiatric and behavioral disturbances(24.6%).In the 159 symptomatic patients lacking c.482G>A variants,the most frequently observed clinical manifestations at disease onset included developmental delay(81.8%),lethargy and feeding difficulty(62.9%),lower limb weakness and poor exercise tolerance(54.7%),prolonged neonatal jaundice(51.6%),vomiting(47.2%),and seizures(32.7%).Before treatment,the levels of blood propionylcarnitine,propionylcarnitine/acetylcarnitine ratio

关键词： c.482G>A CblC disease MMACHC gene Newborn screening Outcome Use our pre-submission checklist Avoid common mistakes on your manuscript.

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