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Effect of SMAD7 gene overexpression on TGF-β1-induced profibrotic responses in fibroblasts derived from Peyronie＇s plaque

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Asian Journal of Andrology 2015年第3期17卷 487-492页

作者： Min ji Choi kang-Moon Song jin-Mi Park Mi-Hye Kwon Ki-Dong Kwon Soo-Hwan Park Dong-Soo ryu ji-kan ryu Jun-Kyu Suh Department of Urology National Research Center for Sexual Medicine Inha University School of Medicine Incheon 400-711 Korea Department of Urology Changwon Samsung Medical Center Changwon 630-522 Korea Inha Research Institute for Medical Sciences Inha University School of Medicine Incheon 400-711 Korea

Transforming growth factor-β1 （TGF-β1） has been identified as one of the most important fibrogenic cytokines associated with Peyronie＇s disease （PD）. The mothers against decapentaplegic homolog 7 （SMAD7） is an inhibitory Smad protein that blocks TGF-J3 signaling pathway. The aim of this study was to examine the anti-fibrotic effect of the SMAD7 gene in primary fibroblasts derived from human PD plaques. PD fibroblasts were pretreated with the SMAD7 gene and then stimulated with TGF-β1. Treated fibroblasts were used for Western blotting, fluorescent immunocytochemistry, hydroxyproline determination, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling assays. Overexpression of the SMAD7 gene inhibited TGF-β1-induced phosphorylation and nuclear translocation of SMAD2 and SMAD3, transdifferentiation of fibroblasts into myofibroblasts, and quashed TGF-β1-induced production of extracellular matrix protein and hydroxyproline. Overexpression of the SMAD7 gene decreased the expression of cyclin D1 （a positive cell cycle regulator） and induced the expression of poly （ADP-ribose） polymerase 1, which is known to terminate Smad-mediated transcription, in PD fibroblasts. These findings suggest that the blocking of the TGF-β pathway by use of SMAD7 may be a promising therapeutic strategy for the treatment of PD.

关键词： cell culture decapentaplegic homolog 7 fibrosis Peyronie's disease transforming growth factor-beta

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Inhibition of histone deacetylase 2 mitigates profibrotic TGF-β1 responses in fibroblasts derived from Peyronie＇s plaque

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Asian Journal of Andrology 2013年第5期15卷 640-645页

作者： ji-kan ryu Woo-Jean Kim Min-ji Choi jin-Mi Park kang-Moon Song Mi-Hye Kwon Nando-Dulal Das Ki-Dong Kwon Dulguun Batbold Guo-Nan Yin Jun-Kyu Suh National Research Center for Sexual Medicine and Department of Urology Inha University School of Medicine Incheon 400-711 Korea

Epigenetic modifications, such as histone acetylation/deacetylation, have been shown to play a role in the pathogenesis of fibrotic disease. Peyronie＇s disease （PD） is a localized fibrotic process of the tunica albuginea, which leads to penile deformity. This study was undertaken to determine the anti-fibrotic effect of small interfering RNA （siRNA）-mediated silencing of histone deacetylase 2 （HDAC2） in primary fibroblasts derived from human PD plaque. PD fibroblasts were pre-treated with HDAC2 siRNA and then stimulated with transforming growth factor-p1 （TGF-β1）. Protein was extracted from treated fibroblasts for Western blotting and the membranes were probed with antibody to phospho-Smad2/Smad2, phospho-Smad3/Smad3, smooth muscle α-actin and extracellular matrix proteins, including plasminogen activator inhibitor-β 1, fibronectin, collagen I and collagen IV. We also performed immunocytochemistry to detect the expression of extracellular matrix proteins and to examine the effect of HDAC2 siRNA on the TGF-β1-induced nuclear translocation of Smad2/3 in fibroblasts. Knockdown of HDAC2 in PD fibroblasts abrogated TGF-β1-induced extracellular matrix production by blocking TGF-β1-induced phosphorylation and nuclear translocation of Smad2 and Smad3, and by inhibiting TGF-β1-induced transdifferentiation of fibroblasts into myofibroblasts. Decoding the individual function of the HDAC isoforms by use of siRNA technology, preferably siRNA for HDAC2, may lead to the development of specific and safe epigenetic therapies for PD.

关键词： cell culture fibrosis histone deacetylase Peyronie's disease transforming growth factor-beta

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Tc-99m ciprofloxacin imaging in diagnosis of chronic bacterial prostatitis

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Asian Journal of Andrology 2003年第3期5卷 179-183页

作者： ji-kan ryu Seong-Min Lee Do-Whan Seong Jun-Kyu Suh Sungeun Kim Wonsick Choe Yeonsook Moon Soo-Hwan Pai Department of Urology Inha University School of MedicineJncheon 400103Korea Department of Nuclear Medicine Inha University School of MedicineIncheon 400103Korea Department of Clinical Patholgy Inha University School of MedicineJncheon 400103Korea

Aim: To investigate the value of Tc-99m ciprofloxacin imaging in the differential diagnosis of chronic bacterial prostatitis. Methods: The study included 4 normal subjects as the negative controls, 2 patients with acute prostatitis or cystourethritis as the positive controls and 59 patients diagnosed as chronic bacterial prostatitis or chronic pelvic pain syndrome by traditional laboratory tests. In every subject, the single photon emission computerized tomography images were obtained 3 h after intravenous injection of Tc-99m Ciprofloxacin. The results of the imaging were compared with those of laboratory tests. Results: On the images, negative uptake was observed in all normal subjects, while strong hot uptake, in the whole prostate of acute prostatitis patients and in the whole urethra of acute cystourethritis patients. In 13 (68 %) of 19 patients categorized as chronic bacterial prostatitis by standard laboratory tests, hot uptake with less intensity than that of acute prostatitis was observed in the prostate area around the prostatic urethra. Negative uptake in the prostate was observed in 6 of 19 patients (32 %) categorized as chronic bacterial prostatitis. Interestingly, hot uptake in the prostate was exhibited in 28 (70 %) of the 40 patients categorized as chronic pelvic pain syndrome. Conclusion: Tc-99m ciprofloxacin imaging is helpful in the differential diagnosis of prostatitis syndrome.

关键词： prostate prostatitis pelvic pain syndrome urinary tract infections radioisotope diagnostic techniques

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Plasma transforming growth factor-β_1 levels in patients with erectile dysfunction

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Asian Journal of Andrology 2004年第4期6卷 349-353页

作者： ji-kan ryu Sun U.Song Hyung-Ki Choi Do-Hwan Seong Sang-Min Yoon Seong-jin Kim Jun-Kyu Suh Department of Urology Clinical Research CenterDepartment of UrologyDepartment of UrologyDepartment of UrologyNational Cancer InstituteDepartment of Urology Inha University School of Medicine Incheon Korea Yonsei University School of Medicine Seoul Korea National Institutes of Health Bethesda MD USA

Aim: To evaluate the plasma TGF-β1 level in erectile dysfunction (ED) patients of various causes. Methods: Sixty-two patients with ED and 26 potent men were subjected to the study. Based on multidisciplinary work-ups, including medical history, physical examinations, blood tests with lipid profile and hormones, penile duplex Doppler ultrasonogram and neurophysiological tests, causes for ED were classified as psychogenic (n=15), neurogenic (n=16) and vasculogenic (n=31). The plasma TGF-β1 level was measured by the ELISA method. Results: The plasma TGF-β1 level was significantly increased in the ED group (6.7 ± 4.9 ng/mL), compared to the control (4.0±2.1 ng/mL) (P <0.01). In the ED groups, there was a significant increase in the vasculogenic group (9.0 ± 5.5 ng/mL), compared to the psychogenic (3.8 ± 1.8 ng/mL) and neurogenic groups (4.8 ± 3.2 ng/mL) (P<0.01). Of the vascular risk factors, both the smoking (7.5 ± 4.7 ng/mL) and dyslipidemia groups (7.4 ± 4.4 ng/mL) showed significantly increased plasma TGF-β1 levels, compared to the non-smokers (5.5 ± 2.8 ng/mL), and those without dyslipidemia (4.8 ?2.8 ng/mL) (P<0.05). Conclusion: Vascular risk factors are associated with an elevated plasma TGF-β1 level, which may contribute to cavernous fibrosis and ED.

关键词： erectile dysfunction transforming growth factor fibrosis diagnosis

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Combination of stromal vascular fraction and Ad-COMP-Angl gene therapy improves long-term therapeutic efficacy for diabetes-induced erectile dysfunction

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Asian Journal of Andrology 2018年第5期20卷 465-472页

作者： Guo-Nan Yin Lin Wang Xiang Nan Lin Lei Shi Zhen-Lin Gao Feng-Chan Han Ping Li Yin-Chuan jin Jun-Kyu Suh ji-kan ryu Xiong Wang Hai Rong jin National Research Center for Sexual Medicine and Department of Urology Inha University School of Medicine Incheon 22332 Korea Department of Urology Yantai Yuhuangding Hospital Affiliated to Medical College of Qingdao University Yantai 264000 China Key Laboratory for Genetic Hearing Disorders in Shandong Binzhou Medical University Yantai 264003 China Department of Histology and Embryology Binzhou Medical University Yantai Shandong 264000 China Reproductive Medicine Center Affiliated Yantai Yuhuangding Hospital of Qingdao University Yantai 264000 China

Men with diabetic erectile dysfunction （ED） respond poorly to the currently available oral phosphodiesterase-5 inhibitors. Therefore, functional therapies for diabetic ED are needed. Stromal vascular fraction （SVF） and the adenovirus-mediated cartilage oligomeric matrix angiopoietin-1 （Ad-COMP-Angl） gene are known to play critical roles in penile erection. We previously reported that SVF and Ad-COMP-Ang1 have only a short-term effect in restoring erectile function. Further improvements to ED therapy are needed for long-lasting effects. In the present study, we aimed to test if the combination of SVF and Ad-COMP-Ang1 could extend the erection effect in diabetic ED. We found that the combination therapy showed a long-term effect in restoring erectile function through enhanced penile endothelial and neural cell regeneration. Combination therapy with SVF and Ad-COMP-Angl notably restored cavernous endothelial cell numbers, pericyte numbers, endothelial cell-cell junctions, decreased cavernous endothelial cell permeability, and promoted neural regeneration for at least 4 weeks in diabetic mice, In summary, this is an initial description of the long-term effect of combination therapy with SVF and Ad-COMP-Ang1 in restoring erectile function through a dual effect on endothelial and neural cell regeneration. Such combination therapy may have therapeutic potential for the treatment of diabetic ED.

关键词： angiopoietin-1 erectile dysfunction neural cell regeneration stromal vascular fraction

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Effects of dispersoid preforming via multistep sintering of oxide dispersion-strengthened CoCrFeMnNi high-entropy alloy

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Journal of Materials Science & Technology 2023年第9期140卷 187-200页

作者： SeungHyeok Chung ji Ho Shin Ho jin ryu Department of Nuclear and Quantum Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea Central Research Institute Korea Hydro and Nuclear Power Co.Ltd.(KHNP)Daejeon 34101Republic of Korea

Dispersoid formation and microstructural evolution in an oxide dispersion-strengthened CoCrFeMnNi high-entropy alloy(HEA)using a newly designed multistep sintering process are *** proposed multistep sintering consists of a dispersoid preforming heat treatment of as-milled 0.1 wt%Y_(2)O_(3)-CoCrFeMnNi high-entropy alloy powders at 800℃,followed by sintering at 800–1000℃ under uniaxial *** the conventional single-step sintered bulk,the coarsened BCC Y_(2)O_(3)dispersoids mainly form with an incoherent interface with the HEA *** contrast,finer FCC Y_(2)O_(3)dispersoids,an atypical form of Y_(2)O_(3),are formed in the matrix region after multistep *** of FCC Y_(2)O_(3)disper-soids is initiated on the favorable facet,the{111}plane of the austenitic matrix,with the formation of a semi-coherent interface with the matrix during the dispersoid preforming heat treatment and it maintains its refined size even after *** is found that dispersoid preforming prior to sintering appears promising to control the finer dispersoid formation and refined grain structure.

关键词： Oxide dispersion strengthening High-Entropy alloy Multistep sintering Dispersoid preforming Microstructure evolution Interfacial structure

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Uniform assembly of gold nanoparticles on S-doped g-C3N4 nanocomposite for effective conversion of 4-nitrophenol by catalytic reduction

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Journal of Materials Science & Technology 2020年第5期40卷 176-184页

作者： Vellaichamy Balakumar Hyungjoo Kim ji Won ryu Ramalingam Manivannan Young-A Son Department of Advanced Organic Materials Engineering Chungnam National University220 Gung-dongYuseong-guDaejeon305-764South Korea

In this work,a simple synthesis of sulfur doped graphitic carbon nitride(S-g-C3N4)act as a support cum stabilizers for gold nanoparticles(Au)and its was characterized by UV–vis and XRD to measure the absorbance and crystallinity,*** functional group and morphology of the samples were identified using FT-IR and ***,the Au@S-g-C3N4 nanocatalyst exhibits good catalytic performance and stability in the reduction of hazardous 4-nitrophenol(NP)compared to S-g-C3N4 using Na ***,the Au@S-g-C3N4 nanocomposite holds a good catalytic efficiency(near 100%)achieved by within 5 *** highest catalytic reduction of NP is due to the synergistic effect of Au nanoparticles decorated on *** fast electron transfer reduction mechanism was elucidated and *** reusability and stability of the developed nanocomposites were also observed in consecutive reduction *** filtering and catalyzing device was used for the direct conversion of NP polluted *** method can open a new avenue for the metal nanoparticles based carbon materials heterogeneous catalyst and its reduction of toxic contaminants.

关键词： Gold nanoparticles S-doped g-C3N4 Catalytic reduction 4-nitrophenol

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Electrochemical performance of all-solid lithium ion batteries with a polyaniline film cathode

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Journal of Energy Chemistry 2016年第1期25卷 93-100页

作者： ji-Woo Oh Rye-Gyeong Oh Yongku kang Kwang-Sun ryu Department of Chemistry University of Ulsan Advanced Materials Divisions Korea Research Institute of Chemical Technology

We have prepared a high-density polyaniline（PANI） paste（50 mg/m L）, with similar physical properties to those of paints or pigments. The synthesis of PANI is confirmed by Fourier transform infrared（FT-IR） spectroscopy. The morphologies of PANI, doped PANI, and doped PANI paste are confirmed by scanning electron microscopy（SEM）. Particles of doped PANI paste are approximately 40–50 nm in diameter, with a uniform and cubic shape. The electrochemical performances of doped PANI paste using both liquid and solid polymer electrolytes have been measured by galvanostatic charge and discharge process. The cell fabricated with doped PANI paste and the solid polymer electrolyte exhibits a discharge capacity of ～87 μAh/cm2（64.0 m Ah/g） at the second cycle and～67 μAh/cm2（50.1 m Ah/g） at the 100 th cycle.

关键词： Lithium ion batteries Cathode Conducting polymer Polyaniline Flexible battery

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Anti-cancer effect of ethylacetate fraction from Orostachys japonicus on HT-29 human colon cancer cells by induction of apoptosis through caspase-dependent signaling pathway

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Asian Pacific Journal of Tropical Medicine 2018年第5期11卷 330-335页

作者： Deok-Seon ryu Hyun-ji Lee ji-Hye Kwon Dong-Seok Lee Department of Biomedical Laboratory Science Soonchunhyang University Department of Smart Foods and Drugs Graduate School of Inje University

Objective: To investigate the anti-colon cancer effects of ethylacetate fraction from Orostachys japonicus(0. japonicus) on HT-29 cancer cells. Methods: The viability of HT-29 cells was assayed by the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2 H-tetrazolium(MTS) method. Apoptosis induction and cell cycle inhibition were confirmed by fluorescein isothiocyanate and propidium iodide staining using flow *** changes in the nucleus were observed, using a fluorescence microscope with4',6-diamidino-2-phenylindole(DAPI) nuclear staining. The expression levels of the upstream and downstream proteins involved in the anti-cancer mechanism were confirmed by Western blotting. Results: After treating HT-29 cells with different concentrations of ethylacetate fraction from O. japonicus, the viability of cells decreased in a concentration-dependent manner,while apoptosis induction and apoptotic body formation increased. Cell cycle analysis showed that the arrest occurred at the sub-G_1 and S phase. Among the upstream and downstream proteins involved in anti-cancer activity, the level of B cell lymphoma-2 decreased, and the bcl-2-associated x protein increased. The level of pro-caspase-3, pro-caspase-8, and pro-caspase-9 decreased, while the level of cleaved-caspase-3, cleaved-caspase-8, and cleaved-caspase-9 increased. Moreover, the phosphorylation, that is, activation of extracellular signal regulated kinase 1/2, Jun-N-terminal kinase, and p38 increased. Conclusions: Combining the above results, it is thought that the survival of HT-29 cells is suppressed by ethylacetate fraction from0. japonicus through mitochondrial regulation-induced caspase cascade activation, induction of apoptosis and cell cycle arrest.

关键词： Orostachys japonicus HT-29 human colon cancer cells Anti-cancer activity Apoptosis Caspase cascade

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Crawlspace heating system in detached house with insulated foundation

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Journal of Central South University 2012年第3期19卷 623-632页

作者：刘青荣阮应君 ryu Yu-ji 任建兴 Institute of Energy and Environmental Engineering Shanghai University of Electric Power School of Mechanical Engineering Tongji University Faculty of Environment Engineering The Unive rsity of Kitakyushu

Two practical crawlspace heating systems introduced in detached houses have been chosen as a field study. One is the crawlspace warm air heating system and the other is the crawlspace hot water circulation system. Based on the field study result, by using the simulation sol, ware, THERB, the effectiveness of the crawlspace warm air heating system has been examined. The effect of the factors, such as the wind amount circulating between crawlspace and indoor space, foundation insulation condition, and heat amount into the crawlspace, on the indoor thermal environment has been analyzed. Based on these analyses, the measured crawlspace heating system can make the average temperature of the living room above 20℃. These two houses have excellent thermal environment. According to the simulating result, heat amount input into crawlspace, which can make comfortable indoor thermal environment, for every month in heating period has been roughly concluded, and they are 600 W in December and March and 800 W in February and January, respectively.

关键词： erawlspaee heating system indoor thermal environment detached house floor insulation foundation insulation

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