检索结果-南通市图书馆

Optimization of adipose tissue-derived mesenchymal stromal cells transplantation for bone marrow repopulation following irradiation

引用

World Journal of Stem Cells 2022年第3期14卷 245-263页

作者： Min-Jung kim Won Moon jeonghoon Heo Sangwook Lim Seung-Hyun Lee Jee-Yeong jeong Sang Joon Lee Department of Biochemistry Cancer Research Institute Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of Internal Medicine Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of Molecular Biology and Immunology Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of Radiation Oncology Kosin University College of MedicineSeo-gu 49267BusanSouth Korea Department of General Surgery Kosin University College of MedicineSeogu 49267BusanSouth Korea Department of Ophthalmology Gospel HospitalKosin University College of MedicineSeo-gu 49267BusanSouth Korea

BACKGROUND Bone marrow(BM)suppression is one of the most common side effects of radiotherapy and the primary cause of death following exposure to *** concerted efforts,there is no definitive treatment method *** studies have reported using mesenchymal stromal cells(MSCs),but their therapeutic effects are *** We administered and examined the effects of various amounts of adipose-derived MSCs(ADSCs)in mice with radiation-induced BM *** Mice were divided into three groups:Normal control group,irradiated(RT)group,and stem cell-treated group following whole-body irradiation(WBI).Mouse ADSCs(mADSCs)were transplanted into the peritoneal cavity either once or three times at 5×10^(5) cells/200μ*** white blood cell count and the levels of,plasma cytokines,BM mRNA,and BM surface markers were compared between the three *** BM-derived CD34+hematopoietic progenitor cells were co-cultured with human ADSCs(hADSCs)or incubated in the presence of hADSCs conditioned media to investigate the effect on human cells in *** The survival rate of mice that received one transplant of mADSCs was higher than that of mice that received three *** transplantations of ADSCs delayed the repopulation of BM hematopoietic stem ***-inflammatory effects and M2 polarization by intraperitoneal ADSCs might suppress erythropoiesis and induce myelopoiesis in sub-lethally RT *** The results suggested that an optimal amount of MSCs could improve survival rates post-WBI.

关键词： Adipose tissue-derived stem cells Bone marrow suppression Mesenchymal stromal cells Radiation Cell therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Combination of cell signaling molecules can facilitate MYOD1-mediated myogenic transdifferentiation of pig fibroblasts

引用

Journal of Animal Science and Biotechnology 2021年第4期12卷 1662-1674页

作者： Jinsol jeong Kwang-Hwan Choi Seung-hun kim Dong-Kyung Lee Jong-Nam Oh Mingyun Lee Gyung Cheol Choe Chang-Kyu Lee Department of Agricultural Biotechnology Animal Biotechnology Majorand Research Institute of Agriculture and Life ScienceSeoul National UniversitySeoul 08826South Korea Present address:Research and Development Center Space F corporationHwasung-siGyeonggi-do 18471South Korea Institute of Green Bio Science and Technology Seoul National UniversityPyeong ChangKangwon-do 25354South Korea

Background:Myogenic transdifferentiation can be accomplished through ectopic MYOD1 expression,which is facilitated by various signaling pathways associated with *** this study,we attempted to transdifferentiate pig embryonic fibroblasts(PEFs)myogenically into skeletal muscle through overexpression of the pig MYOD1 gene and modulation of the FGF,TGF-β,WNT,and cAMP signaling ***:The MYOD1 overexpression vector was constructed based on comparative sequence analysis,demonstrating that pig MYOD1 has evolutionarily conserved domains across various *** forced MYOD1 expression through these vectors triggered the expression of endogenous muscle markers,transdifferentiated muscle cells from fibroblasts were not ***,various signaling molecules,including FGF2,SB431542,CHIR99021,and forskolin,along with MYOD1 overexpression were applied to enhance the myogenic *** modified conditions led to the derivation of myotubes and activation of muscle markers in PEFs,as determined by qPCR and ***,a sarcomere-like structure was observed,indicating that terminally differentiated skeletal muscle could be obtained from transdifferentiated ***:In summary,we established a protocol for reprogramming MYOD1-overexpressing PEFs into the mature skeletal muscle using signaling *** myogenic reprogramming can be used as a cell source for muscle disease models in regenerative medicine and the production of cultured meat in cellular agriculture.

关键词： MYOD1 Pig Sequence analysis Skeletal muscle Transdifferentiation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Enzyme-amplified SERS immunoassay with Ag-Au bimetallic SERS hot spots

引用

Nano Research 2020年第12期13卷 3338-3346页

作者： Xuan-hung Pham Eunil Hahm Tae Han kim Hyung-Mo kim Sang hun Lee Sang Chul Lee Homan Kang Ho-Young Lee Dae Hong jeong Hak Soo Choi Bong-Hyun Jun Department of Bioscience and Biotechnology Konkuk UniversitySeoul143-701Republic of Korea Department of Nuclear Medicine Seoul National University Bundang HospitalSeongnam13620Republic of Korea Gordon Center for Medical Imaging Department of RadiologyMassachusetts General Hospital and Harvard Medical SchoolBostonMA02114USA Department of Chemistry Education Seoul National UniversitySeoul151-742Republic of Korea

Surface-enhanced Raman scattering(SERS)enables rapid detection of single molecules with high ***,quantitative and sensitive SERS analysis has been a challenge due to the lack of reliable SERS-active *** this study,we developed a quantitative SERS-based immunoassay using enzyme-guided Ag growth on Raman labeling compound(RLC)-immobilized gold nanoparticle(Au NP)-assembled silica NPs(SiO2@Au-RLC@Ag).The enzyme amplified Ag+reduction as well as Ag growth on the RLC-immobilized Au NP-assembled silica NPs(SiO2@Au-RLC),which resulted in a significant increase in SERS *** the presence of target antigens such as immunoglobulinG(IgG)or prostate-specific antigen(PSA),Ab1-Antigen-Ab2 immune complex with alkaline phosphatase triggered an enzyme-catalyzed reaction to convert 2-phospho-L-ascorbic acid(2-phospho-L-AA)to ascorbic acid(AA).As produced AA reduced Ag+to Ag,forming an Ag hot spot on the surface of SiO2@Au-RLC,which enhanced the SERS signal of SiO2@Au-RLC@Ag in a solution with a target antigen *** plasmonic immunoassay for IgG detection showed a high linearity of SERS intensity in the range of 0.6 to 9.0 ng/mL with a detection limit(LOD)of 0.09 ng/mL,while an LOD of 0.006 ng/mL was obtained for *** results indicate that the sensitivity of our novel SERS-based immunoassay is higher than that of conventional enzyme-based colorimetric immunoassays.

关键词： surface-enhanced Raman scattering(SERS)-based immunoassay Au-Ag alloy surface-enhanced Raman scattering silica template immunoglobulin G(IgG)detection

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pharmacokinetic comparison with different assays for simultaneous determination of cis-,trans-cefprozil diastereomers in human plasma

引用

Journal of Pharmaceutical Analysis 2021年第3期11卷 351-363页

作者： Seung-Hyun jeong Ji-hun Jang Hea-Young Cho Yong-Bok Lee College of Pharmacy Chonnam National University77 Yongbong-roBuk-guGwangju61186Republic of Korea College of Pharmacy CHA University335 Pangyo-roBundang-guSeongnam-siGyeonggi-Do13488Republic of Korea

The purpose of this study was to compare pharmacokinetic(PK)parameters obtained using two newly developed assays,HPLC-UV and UPLC-ESI-MS/*** of assay and results obtained therefrom are very important in PK studies and can have a major impact on the PK-based clinical dose and usage *** this study,we developed two new methods that are most commonly used in biosample analysis and focused on PK parameters obtained from *** HPLC-UV equipped with a Luna-C8 column using UV detector,cefprozil diastereomers were separated using water containing 2%(V/V)acetic acid and acetonitrile as a mobile *** UPLC-ESI-MS/MS equipped with a HALO-C18 column,cefprozil diastereomers were separated using 0.5%(V/V)aqueous formic acid containing 5 mM ammoniumformate buffer and methanol as a mobile *** showed high resolution,sensitivity,and selectivity without interference by plasma *** intra-and inter-day precisions(CV,%)were within 8.88%for HPLC-UV and UPLC-ESI-MS/*** of both methods was 95.67%-107.50%.These two analytical methods satisfied the criteria of international guidance and could be successfully applied to PK *** of PK parameters between two assays confirmed that there is a difference in the predicted minimum plasma concentrations at steady state,which may affect clinical dose and usage ***,we confirmed possible correlation between PK parameters and various biochemical parameters after oral administration of 1000 mg cefprozil to humans.

关键词： Cefprozil diastereomers Comparison HPLC-UV UPLC-ESI-MS/MS Pharmacokinetic parameter Biochemical parameters

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Acute liver injury induces expression of FGF23 in hepatocytes via orphan nuclear receptor ERRγ signaling

引用

Genes & Diseases 2023年第3期10卷 679-682页

作者： Yoon Seok Jung Yong-Hoon kim Kamalakannan Radhakrishnan Jung-Ran Noh Jung Hyeon Choi Hyo-Jin kim Jae-Ho jeong Steven Dooley Chul-Ho Lee Hueng-Sik Choi School of Biological Sciences and Technology Chonnam National UniversityGwangju 61186Republic of Korea Laboratory Animal Resource Center Korea Research Institute of Bioscience and Biotechnology125 Gwahak-roYuseong-guDaejeon 34141Republic of Korea Department of Functional Genomics KRIBB School of BioscienceKorea University of Science and Technology(UST)Daejeon 34113Republic of Korea Combinatorial Tumor Immunotherapy MRC Chonnam National University Medical SchoolHwasun-gunJeonnam 58128Republic of Korea Department of Microbiology Chonnam National University Medical SchoolGwangju 61186Republic of Korea Molecular Hepatology Section Medical Faculty MannheimHeidelberg University69117 HeidelbergGermany

Fibroblast growth factor 23(FGF23)is an osteocyte-and osteoblast-derived hormone that primarily regulates phosphate and vitamin D *** FGF23 levels are abnormally increased in pathological conditions like acute or chronic kidney injury,resulting in disease progression as well as increased rates of morbidity and mortality.^(1) However,FGF23 production in acute liver injury is not fully *** this study.

关键词： FGF23 acute vitamin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Repetitive response to gemcitabine that led to curative resection in cholangiocarcinoma

引用

World Journal of Gastroenterology 2009年第36期15卷 4593-4595页

作者： Seong hun kim In Hee kim Sang Wook kim Seung-Ok Lee Division of Gastroenterology and Hepatology Department of Internal MedicineChonbuk National University Medical SchoolJeonju 561-712South Korea

This study reports a case of unresectable intrahepatic mass-forming cholangiocarcinoma which showed a dramatic response to gemcitabine that led to curative resection and a long-term survival of more than five *** and five cycles of gemcitabine monotherapy were administered separately over a three-year period and a radical excision was performed at 4.5 years after *** case indicates the role of gemcitabine as a neoadjuvant chemotherapeutic agent for cholangiocarcinoma and guarantees a randomized controlled prospective study.

关键词：胆管反应手术癌质量情况化疗药物治疗给药

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of pulse time on surface characteristics and corrosion resistance during pulse electrochemical polishing

引用

中国有色金属学会会刊：英文版 2012年第S3期22卷 876-880页

作者： Young-Bin kim jeong-Woo PARK Department of Advanced Parts and Materials Engineering Chosun University Department of Mechanical Design Engineering Chosun University

Pulse electrochemical polishing (PECP) was used to improve the mechanical properties,such as surface roughness and corrosion resistance,of conductive metallic *** can provide a smooth,bright,reflective,and deburred surface that exhibits superior corrosion *** this work,stainless steel was used as the anode,and copper was used as the cathode due to their low electrical *** surface roughness of the PECP sample was measured by atomic force microscopy (AFM).A scanning electron microscope (SEM) was used to observe surface characteristics,and an Auger electron spectroscope (AES) was used to analyze the metallurgical composition and thickness of the passive *** aim of this research was to compare the corrosion resistance rates of the unprocessed and PECP-processed stainless steel.

关键词： stainless stell pulse electrochemical polishing corrosion resistance

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of focal laser photocoagulation in eyes with mild to moderate non-proliferative diabetic retinopathy

引用

International Journal of Ophthalmology(English edition) 2016年第10期9卷 1439-1443页

作者： Seong hun jeong Jung Il Han Sung Won Cho Dong Won Lee Chul Gu kim Tae Gon Lee Jong Woo kim Department of Ophthalmology Kim's Eye HospitalMyung-Gok Eye Research InstituteKonyang University College of Medicine

AIM：To report the effect of focal laser photocoagulation on both the severity of hard exudates（HEs） and the rate of disease progression in eyes with mild to moderate non-proliferative diabetic retinopathy（NPDR）.METHODS： We retrospectively reviewed the medical records of 33 patients（60 eyes） who had been diagnosed with mild to moderate NPDR between January 2006 and December *** patients were divided into 2 groups：Group A（38 eyes in 20 patients treated using focal laser photocoagulation） and Group B（treated without laser photocoagulation）.We also reviewed the best corrected visual acuity measurements,and the fundus photographs taken at both baseline and follow-up visits. RESULTS： In Group A,HE severity grade had decreased significantly from baseline to the final visit（P 〈0.05）,but this was not the case in Group B（P =0.662）.The cumulative probabilities of retinopathy progression at 5y were 26% in Group A and 30% in Group *** survival curves showed no significant difference between the groups with regard to retinopathy progression（P =0.805）.CONCLUSION： Focal laser photocoagulation reduced the levels of HEs in eyes with mild to moderate ***,the treatment was not able to decelerate the progression of DR.

关键词： focal laser diabetic retinopathy retina non-proliferative diabetic retinopathy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Surgical treatment of liver metastasis with uveal melanoma:A case report

引用

World Journal of Clinical Cases 2021年第28期9卷 8498-8503页

作者： Young-hun kim Nam-Kyu Choi Department of Surgery Chosun University HospitalGwangju Metropolitan City 61453South Korea Department of Surgery Division of Hepatobiliary Surgery and Liver TransplantationChosun University HospitalGwangju Metropolitan City 61453South Korea

BACKGROUND Uveal melanoma is the most common primary intraocular malignant tumor affecting the eyes in *** half of all primary uveal melanoma tumors metastasize;yet,there are currently no effective treatments for metastatic uveal *** the time of diagnosis,less than 4%of patients with uveal melanoma have detectable metastatic *** melanoma disseminates hematogenously,with the most common site of metastasis being liver(93%),followed by lung(24%)and bone(16%).CASE SUMMARY A 57-year-old woman was diagnosed with a dysplastic nevus on her eyelid,which was histologically confirmed as malignant melanoma after *** patient had no evidence of metastasis to other organs and received both radiation therapy and *** systemic treatment,a metastatic left neck lymph node was found and another round of chemotherapy was performed after *** emission tomography-Computed Tomography tracking after completion of chemotherapy revealed two metastatic liver *** patient underwent partial liver resection and showed no signs of recurrence at 1 year after *** Surgery is an effective treatment for metastatic uveal *** patients with liver metastatic lesions,hepatectomy improves outcome.

关键词： Uveal melanoma Metastatic melanoma Liver metastasis Hepatectomy Prognosis Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Nozzle Geometry on the Flow Characteristics of Spiral Flow Generated through an Annular Slit

引用

Journal of Thermal Science 2007年第2期16卷 149-154页

作者： Shigeru MATSUO Tae-hun kim Toshiaki SETOGUCHI Heuy Dong kim Yeon-Won LEE Department of Mechanical Engineering Saga University1Honjo-machiSaga-shiSaga 840-8502Japan Graduate school of Science and Technology Saga University1Honjo-machiSaga shiSaga 840-8502Japan School of Mechanical Engineering Andong National University388Songchun-dongAndong 760749Korea School of Mechanical Engineering College of EngineeringPukyong National UniversitySan 100Young-Dang DongBusanKorea

与打漩的以前的研究比较流动，在螺线嘴产生的螺线流动有一些不同流动特征。产生它的速度不需要强迫的正切的动量并且从象长潜在的核心和低正切的速度那样的打漩的流动有一些不同优点。在这研究，在螺线嘴的流动特征上的嘴几何学的效果... 详细信息

与打漩的以前的研究比较流动，在螺线嘴产生的螺线流动有一些不同流动特征。产生它的速度不需要强迫的正切的动量并且从象长潜在的核心和低正切的速度那样的打漩的流动有一些不同优点。在这研究，在螺线嘴的流动特征上的嘴几何学的效果被实验和计算调查。作为结果，实验获得的流动特征与计算结果，份量上和品质上有一个令人满意的协议。

关键词：螺旋流流动特性喷嘴几何形状环形裂缝

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：