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Site-Specific Conjugation of Cell Wall Polyrhamnose to Protein SpyAD Envisioning a Safe Universal Group A Streptococcal Vaccine

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Infectious microbes & Diseases 2021年第2期3卷 87-100页

作者： Nina J.Gao Satoshi Uchiyama Lucy Pill Samira Dahesh Joshua Olson Leslie Bautista Shilpa maroju Aym Berges Janet ZLiu Raymond HZurich Nina mvan Sorge jeff Fairman Neeraj Kapoor Victor Nizet Division of Host-microbe Systems and Therapeutics Department of PediatricsUC San DiegoLa JollaCaliforniaUSA Vaxcyte Inc.Foster CityCaliforniaUSA Department of medical microbiology and Infection Prevention Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Netherlands Reference Laboratory for Bacterial meningitis Amsterdam University Medical CenterUniversity of AmsterdamAmsterdamThe Netherlands Skaggs School of Pharmacy and Pharmaceutical Sciences UC San DiegoLa JollaCaliforniaUSA

Development of an effective vaccine against the leading human bacterial pathogen group A Streptococcus(GAS)is a public health *** species defining group A cell wall carbohydrate(GAC,Lancefield antigen)can be engineered to remove its immunodominant N-acetylglucosamine(GlcNAc)side chain,implicated in provoking autoimmune cross-reactivity in rheumatic heart disease,leaving its polyrhamnose core(GACPR).Here we generate a novel protein conjugate of the GACPR and test the utility of this conjugate antigen in active *** of conjugation to a standard carrier protein,we selected SpyAD,a highly conserved GAS surface protein containing both B-cell and T-cell epitopes relevant to the bacterium that itself shows promise as a vaccine *** was synthesized using the XpressTm cell-free protein expression system,incorporating a non-natural amino acid to which GACpr was conjugated by site-specific click chemistry to yield high molecular mass SpyAD-GACPR conjugates and avoid disruption of important T-cell and B-cell immunological *** conjugated SpyAD-GACPR elicited antibodies that bound the surface of multiple GAS strains of diverse m types and promoted opsonophagocytic killing by human *** immunization of mice with a multivalent vaccine consisting of SpyAD-GACPR,together with candidate vaccine antigens streptolysin O and C5a peptidase,protected against GAS challenge in a systemic infection model and localized skin infection model,without evidence of cross reactivity to human heart or brain tissue *** general approach may allow GAC to be safely and effectively included in future GAS subunit vaccine formulations with the goal of broad protection without autoreactivity.

关键词： group A Streptococcus Streptococcus pyogenes group A carbohydrate Lancefield antigen conjugate vaccine XpressCFTm non-natural amino acids SpyAD Introduction

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利用视频均衡器延长RS-422的传输距离

世界电子元器件 2006年第10期 40-41页

作者： jeff Lies Intersil公司

TI/EIA-422-B的指标建议，5mbps的数据速率要在CAT5类的电缆上实现无故障运行，其传输距离不得超过30m（98英尺）。显然，这是一个相当保守的数字，不过，即使是将其变为原来的3倍，也才不过是90米（或295英尺）。

关键词：传输距离 RS-422 均衡器视频无故障运行数据速率

Potential of a planarian model to study certain aspects of anti-Parkinsonism drugs

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Advances in Parkinson's Disease 2013年第3期2卷 70-74页

作者： Robert B. Raffa jeff Danah Christopher S. Tallarida Carrie Zimmerman Grace Gill Steven J. Baron Scott m. Rawls Center for Substance Abuse Research and Temple University School of medicine Philadelphia USA Temple University School of Pharmacy Philadelphia USA

We previously created and investigated a planarian model for the study of drug action, abuse, physical dependence, receptor affinity, the toxicity of heavy metals in wastewater, and seizures. For the present pilot study, we investigated the possibility that this model might be useful for studying certain aspects of drugs used in treatment of Parkinson disease. For the first step, we were interested in finding an in vivo metric for the inhibition of L-DOPA by an inhibitor of DOPA decarboxylase. The direct clinical relevance of the endpoint was of secondary concern during this preliminary phase of model development. Two metrics were explored: L-DOPA-induced inhibition of motility (locomotor velocity) and dopamine-mediated toxicity, which was quantified using a Kaplan-meier survival curve. L-DOPA produced both dose- and time-related toxicity. The water-soluble DOPA decarboxylase inhibitor benserazide dose-dependently inhibited the effect of L-DOPA, as manifested by a leftward shift in the Kaplan-meier curve. Additional work was initiated using the more sensitive and a graded metric of spontaneous locomotor velocity. The encouraging results of this pilot study suggest that: 1) planarians contain DOPA decarboxylase or an equivalent enzyme, and 2) the planarian model might be useful for the study of certain aspects of anti-Parkinsonism pharmacotherapy.

关键词： L-DOPA Benserazide Planarians

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为什么说人民币被高估了

中国改革 2010年第12期 92-93页

作者： jeff Lu 耶鲁大学北京俱乐部

中国m2和GDP增长之差为42％，而美国为19％，人民币是不是应该相对于美元贬值23％？

关键词：人民币高估美元贬值 GDP

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创新决胜——全球最具创新力企业50强

国际商业技术 2012年第1期 68-71页

作者： jeff Dyer Hal B Gergersen Clayton m．Christensen 朱娱（摘译）杨百翰(Brigham Young)大学万豪学院战略研究系欧洲工商管理学院(INSEAD)领导科学系哈佛大学商学院企业管理系

创新力是全球经济的生命血液，也是所有的企业领军者所必须掌握的战略先机。革命性的创新既能打败整个行业，也能创造无边财富，一些经典的案例我们耳熟能详：苹果的iPod终结了索尼的随身听；星巴克的咖啡和舒适空间吞噬了街角的小咖啡... 详细信息

创新力是全球经济的生命血液，也是所有的企业领军者所必须掌握的战略先机。革命性的创新既能打败整个行业，也能创造无边财富，一些经典的案例我们耳熟能详：苹果的iPod终结了索尼的随身听；星巴克的咖啡和舒适空间吞噬了街角的小咖啡屋；Skype运用“免费”的战略横扫美国电报电话公司（AT＆T）。

关键词：创新力企业美国电报电话公司 Skype 全球经济 iPod 咖啡屋革命性

用LandSat—4专题制图仪推求雪的反射率

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遥感技术与应用 1987年第2期 65-69页

作者： jeff DOZIER 宋强

在美国加州,75％的农业用水来自内华达山上积雪场的融化。用专题制图仪所获得的该流域范围内的光谱反射率,可较好地进行春季的融雪径流预报。鉴于专题制图仪具有较大的动态响应,故在积雪场研究中,它比多波段扫描仪优越。在Tm1—4波段中... 详细信息

在美国加州,75％的农业用水来自内华达山上积雪场的融化。用专题制图仪所获得的该流域范围内的光谱反射率,可较好地进行春季的融雪径流预报。鉴于专题制图仪具有较大的动态响应,故在积雪场研究中,它比多波段扫描仪优越。在Tm1—4波段中,也存在着饱和现象,不过仅是Tm1(0.45μm—0.52μm)较为严重。雪在Tm2(0.53μm—0.61μm)上的反射率可以做为整个可见光波段上的反射率的代表,此波段的反射率对雪的晶体颗粒大小几乎没有什么显著的反应,但对雪的污染极为敏感。Tm4(0.78μm—0.9μm)可用于估算雪的有效光学粒径。Tm5(1.57μm—1.78μm)可以把云从雪中分辨出来。

关键词：反射率专题制图仪 Tm 反射比率波段 LandSat

Impact of manure Storage Time and Temperature on microbial Composition and Stable Fly (Diptera: muscidae) Development

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Advances in microbiology 2019年第3期9卷 248-265页

作者： Janice L. Speshock jeff A. Brady Jessica Eastman Travis Roach Samantha Hays David Kattes Department of Biology Tarleton State University Stephenville USA Department of Wildlife Sustainability & Ecosystem Sciences Tarleton State University Stephenville USA Texas A&m AgriLife Research & Extension Center Stephenville USA Department of Agriculture & Environmental Science Tarleton State University Stephenville USA

Samples are often frozen for preservation until needed for use. It has been a common practice to store fresh dairy manure in the freezer until needed for fly development studies. However, conflicting data have suggested that freezer temperature and duration of manure may impact fly development studies, and it is likely due to the change in microbial comminutes due to the freezer conditions. In this study manure storage conditions were assessed to ascertain how temperatures impact stable fly, Stomoxys calcitrans L., survival to pupation and determine which bacterial populations impacted fly development using massively-parallel sequencing and 16S metagenomic analysis. Stable fly survival to pupation was greater in manure that was stored warm (27?C) or frozen (-20?C or -80?C) for 24 days as compared to fresh manure samples. Refrigeration (4?C) of the manure for 24 days did not affect fly development and slightly decreased the pupal weights. Over 80 bacterial families were detected by sequencing allowing for a more thorough assessment of changes in bacterial populations. Only minor shifts were observed in bacterial family composition in the manure when refrigerated or frozen for 24 days, but significant population changes were observed when the manure was incubated for 24 days at 27?C. Since it is the temperature and incubation time that yielded the greatest pupation rate, it is hypothesized that the manure microbial community impacts the growth and development of stable flies. This study has determined suggested freezer conditions for the best storage of manure samples to maintain bacterial diversity and retain the closest bacterial populations to freshly collected manure. Although untouched, aged (20 days) manure is best to use to assess fly development, it is not always feasible in laboratory experimentations. This study demonstrates the importance of preservation techniques on manure samples, which could also confer to storage of other biological specimens that cont

关键词： Stomoxys calcitrans L. manure microbiota 16S metagenomics

Toward a biomarker panel measured in CNS-originating extracellular vesicles for improved differential diagnosis of Parkinson’s disease and multiple system atrophy

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Translational Neurodegeneration 2023年第1期12卷 677-680页

作者： Hash Brown Taha Simon Hornung Suman Dutta Leony Fenwick Otmane Lahgui Kathryn Howe Nour Elabed Irish del Rosario Darice Y.Wong Aline Duarte Folle Daniela markovic Jose-Alberto Palma Un Jung Kang Roy N.Alcalay miriam Sklerov Horacio Kaufmann Brent L.Fogel jeff m.Bronstein Beate Ritz Gal Bitan Department of Neurology David Geffen School of MedicineUniversity of California Los AngelesLos AngelesCA 90095USA Department of Integrative Biology and Physiology University of California Los AngelesLos AngelesCA 90095USA Department of Epidemiology Fielding School of Public HealthUniversity of California Los AngelesLos AngelesCA 90095USA Department of medicine Statistics Core Division of General Internal Medicine and Health Services ResearchUniversity of California Los AngelesLos AngelesCA 90095USA Department of Neurology Dysautonomia CenterNew York University School of MedicineNew YorkNY 10016USA Department of Neurology Taub Institute for Research on Alzheimer’s Disease and the Aging BrainColumbia UniversityNew YorkNY 10032USA Department of Neurology Tel Aviv Sourasky Medical CenterTel AvivIsrael Department of Neurology University of North Carolina School of MedicineChapel HillNC 27599USA Department of Neurology The Marlene and Paolo Fresco Institute for Parkinson’s and Movement DisordersNew York University School of MedicineNew YorkNY 10016USA Department of Human Genetics David Geffen School of MedicineUniversity of California Los AngelesLos AngelesCA 90095USA UCLA Clinical Neurogenomics Research Center David Geffen School of MedicineUniversity of CaliforniaLos AngelesCA 90095USA Brain Research Institute University of CaliforniaLos AngelesCA 90095USA molecular Biology Institute University of California Los AngelesLos AngelesCA 90095USA Present Address:Division of Peptide Biochemistry TUM School of Life SciencesTechnical University of Munich85354 FreisingGermany

Synucleinopathies are neurodegenerative diseases char-acterized by accumulation of misfoldedα-synuclein(α-syn)inclusions in neuronal and/or glial *** differences in the underlying pathophysiology,synucle-inopathies ... 详细信息

关键词： diagnosis atrophy diseases

The Effect of Catena Position on Greenhouse Gas Emissions from Dambo Located Termite (<i>Odontotermes transvaalensis</i>) mounds from Central Zimbabwe

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Atmospheric and Climate Sciences 2012年第4期2卷 501-509页

作者： George Nyamadzawo Jephita Gotosa Justice muvengwi menas Wuta Justice Nyamangara Philip Nyamugafata jeff L. Smith Department of Environmental Science Bindura University of Science Education Bindura Zimbabwe Department of Soil Science and Agricultural Engineering University of Zimbabwe Mount Pleasant Harare Zimbabwe International Centre for Research in Semi Arid Tropics (ICRISAT) matopos Bulawayo Zimbabwe USDA-Agricultural Research Service Washington DC USA

methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2)O) are greenhouse gases (GHGs) which cause global warming. Natural sources of GHGs include wetlands and termites. Previous studies have quantified GHG emissions from upland termites and no study has reported GHG emissions from seasonal wetlands (dambo) located termite mounds. The objective of this study was to evaluate the effect of dambo catena position on termite mound distribution and GHG emissions. It was hypothesized that mound density and GHG emissions from Odontotermes transvaalensis mounds, vary with catena position. The evaluated catena positions were margin, mid-slope, lower slope and bottom. mound density was significantly lower in the bottom when compared to the other catena positions. The mean GHG fluxes were 88 μg m2 hr-1, 0.78 mg m2 hr-1 and 1361 mg m2 hr-1 for N2) O, CH4 and CO2 respectively. Fluxes varied with catena position and were 0.48, 0.72, 1.35 and 0.79 mg m-2 hr-1 for CH4 , and 1173.7, 1440.7, 1798.7 and 922.8 mg m-2 hr-1 for CO2 in the margin, mid-slope, lower slope and the bottom catena position respectively. For N2) O, there were no significant differences between catena positions. It was concluded that dambo located Odontotermes transvaalensis termite mounds are an important source of GHGs, and emissions varied with catena position for CO2 and CH4.

关键词： Greenhouse Gas Emissions Termites mounds Odontotermes transvaalensis Dambos Catena Position