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Epidemiology of carbapenem-resistant Acinetobacter baumannii colonization in neonatal intensive care units:A systematic review and meta-analysis

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World Journal of Meta-Analysis 2024年第1期12卷 1-10页

作者： Donatien Serge Mbaga Sebastien Kenmoe Seraphine Nkie Esemu Arnol Bowo-Ngandji Nene Kaah Keneh Jane-Francis Tatah Kihla Akoachere Hortense Kamga Gonsu Roland Ndip Ndip jean Thierry Ebogo-Belobo Cyprien Kengne-Ndé Nicholas Tendongfor jean paul assam assam Lucy Mande Ndip Jacky Njiki Bikoï Sara Honorine Riwom Essama Department of Microbiology The University of Yaounde IYaounde 00237Cameroon Department of Microbiology and Parasitology University of BueaBuea 00237Cameroon Faculty of Medicine and Biomedical Sciences The University of Yaounde IYaounde 00237Cameroon Center for Research in Health and Priority Pathologies Institute of Medical Research and Medicinal Plants StudiesYaounde 00237Cameroon Epidemiological Surveillance Evaluation and Research UnitNational AIDS Control CommitteeDouala 00237Cameroon

BACKGROUND The rising prevalence of carbapenem-resistant Acinetobacter baumannii(CRAB)in neonatal intensive care units(NICUs)represents an escalating challenge in healthcare settings,particularly in managing hospital-acquired infections(HAIs).Studies across various World Health Organization regions have documented a significant incidence of CRAB-related HAIs,with rates as high as 41.7 cases per 1000 patients in ICUs,accounting for 13.6%of all *** infections pose a doubled mortality risk compared to infections with carbapenem-susceptible Acinetobacter baumannii.A particularly concerning aspect of CRAB colonization is its asymptomatic nature,enabling its transmission through healthcare workers(HCWs)or the NICU environment to vulnerable neonates with developing immune *** To explore the prevalence of CRAB colonization in NICUs,focusing on neonates,healthcare workers,and the environmental samples,to enhance epidemiological understanding and inform targeted *** We conducted according to PRISMA 2020 checklist guidelines,a comprehensive literature search across multiple databases including MEDLINE(Ovid),EMBASE(Ovid),Global Health(Ovid),Web of Science,and Global Index *** were selected based on predetermined criteria,primarily involving neonates,HCWs,and environmental swabs,using culture or molecular methods to detect CRAB *** excluded studies that did not specifically focus on NICUs,were duplicates,or lacked necessary *** study selection and quality assessment were conducted independently by two *** extraction involved collecting comprehensive details about each *** statistical analysis used a random-effects model to calculate the pooled prevalence and confidence intervals,stratifying results by regional *** assessed study heterogeneity using Cochran's Q statistic and I²statistic,with regression tests employed to evaluate potential publication *** We analyzed 737 records from f

关键词： Colonization Carbapenem-resistant Acinetobacter baumannii Neonatal intensive-care unit

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Comparison of the Sensitivities of Different PCR Assays for the Detection of Mycobacterium tuberculosis Complex Isolates from Five Regions of Cameroon

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Journal of Tuberculosis Research 2015年第2期3卷 34-42页

作者： Fidelis Cho-Ngwa Irene A. Anyangwe Emiliene T. Berinyuy Henry D. Meriki jean-paul assam-assam Vincent P. Titanji Biotechnology Unit Faculty of Science University of Buea Buea Cameroon Laboratory of Tuberculosis Research Biotechnology Centre of Nkolbisson Faculty of Science University of Yaoundé I Yaoundé Cameroon

The accurate diagnosis of tuberculosis caused by members of the Mycobacterium tuberculosis complex (MTBC) has remained a major challenge in clinical laboratories world-wide. Several studies have evaluated the use of highly specific in-house PCR assays targeting the IS6110, hupB, rpoB, oxyR, and IS1081 genes in the detection of MTBC species with reports on variable sensitivities depending on the geographical sourcing of isolates. In the present investigations, we evaluated the sensitivities of these PCR assays on 125 MTBC cultured isolates from five (West, Centre, Littoral, North West and South West) of the ten regions of Cameroon. Of this number, 124 (99.2%), 117 (93.6%), 123 (99.1%), 119 (95.2%) and 118 (94.4%) were positive by the IS6110, hupB, rpoB, oxyR, and IS1081-based PCR assays respectively. A total of 110 (88%) of the cultured isolates were also identified as MTBC by standard biochemical tests. Of this number, 109 (99.1%), 104 (94.5%), 109 (99.1%), 106 (96.4%) and 104 (94.5%) were positive in the IS6110, hupB, rpoB, oxyR, and IS1081-based PCR assays respectively. Concordant PCR results were obtained for 108 of the 125 samples. The 15 isolates that were negative biochemically scored sensitivities ranging from 100% (for the IS6110 assay) to 86.7% (for the hupB and oxyR assay). The combination of the IS6110 assay, which turned out to be the most sensitive, and each of the other assays gave 100% sensitivity. We conclude that the combined targeting of the IS6110 and rpoB genes is likely to yield the most sensitive PCR procedure for the diagnosis of MTBC infection in the five regions of Cameroon.

关键词： PCR-Assays Sensitivities MTB Complex

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Estimates of Genetic Variability of <i>Mycobacterium tuberculosis</i>Complex and Its Association with Drug Resistance in Cameroon

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Advances in Infectious Diseases 2013年第1期3卷 55-59页

作者： Larissa Kamgue Sidze Emmanuel Mouafo Tekwu Christopher Kuaban jean-paul assam assam jean-Claude Tedom Stefan Niemann Matthias Frank Véronique N. Penlap Beng Institute for Tropical Medicine University of Tübingen Tübingen Germany Laboratory for Tuberculosis Research Biotechnology Centre of Nkolbisson (BTC) University of Yaoundé I Yaoundé Cameroon Pneumology Unit Jamot Hospital Yaoundé Cameroon Research Centre Borstel Molecular Mycobacteriology Borstel Germany

The present study investigates the genetic diversity among Mycobacterium tuberculosis complex circulating in the Centre region of Cameroon and analyzes the relationship between genotypes and drug resistance patterns. Spoligotyping was performed by PCR-amplification followed by the reverse hybridization of 298 cultured specimens. Spoligotypes patterns were identified by comparison to reference strains in SPolDB4 database via the MIRU VNTR plus web application. About 97.65% of all tuberculosis (TB) cases were attributed to M. tuberculosis. A total of 65 different profiles were identified. Of these, 40 were represented as Shared Types (ST) while the others were orphans. LAM10_CAM and Haarlem families were the most prevalent genetic families with 51.01% and 14.09% respectively. ST 61, a member of the LAM10_ CAM family formed the largest cluster with 128 (42.95%) isolates. No association was found between genotypes with regard to drug resistance and HIV sero-status. However, there was a significant association between genotypes and age groups. Patients belonging to 15 - 24 and 35 - 44 age groups were more likely infected by LAM10_CAM strains compared to others. The population structure of Mycobacterium tuberculosis complex strains from the Centre region was found to be diverse and the spoligotype 61 of the LAM10_CAM family was highly predominant. Isolates of the LAM10_CAM seem to be not associated with drug resistance.

关键词： M. tuberculosis Spoligotyping LAM10_CAM

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A Global Group of Friends

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Beijing Review 2024年第29期67卷 26-27页

作者： jean paul Vargas the Sustainable Development Innovation Laboratory Central American Institute of Public Administration the Center for Historical Research of Central America University of Costa Rica

China's Global Development Initiative(GDI)is emerging as a transformative force in the international *** President Xi Jinping first publicized the conceplal the United Nations General Assembly in September *** its announcemen,the GDT's main components and objectives,aimed at fostering cunnectily and coperation between coninents,hawe atractod workdwide atentin.

关键词： Global foster objectives

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Fuzzy Henstock-Kurzweil Triple Integral on a Type 1 Quasi-Fuzzy Parallelepipedal Domain

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Journal of Applied Mathematics and Physics 2024年第3期12卷 897-911页

作者： Kumwimba Seya Didier Kasongo Ntambwe Dany Panga Lutanda Grégoire Mayuke Katshongo jean paul Lunda Ngoy jean Pierre Département de Mathématiques et Informatique Faculté des Sciences Université de Lubumbashi Lubumbashi Democratic Republic of the Congo

In this article, we propose by using the Hausdorff distance Simpson’s rule for the triple integral of a fuzzy-valued function and the error bound of this method, one of the variables of which is fuzzy. In addition, thin δ-fine partitions are introduced. The integration domain is a quasi-fuzzy parallelipiped. A numerical example is presented in order to show the application and the significance of the method.

关键词： Fuzzy-Valued Function Hausdorff Distance Quasi-Fuzzy Parallelipiped Henstock Integral

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Fatty Acid Composition of Hazelnut Kernel Oil from Coula edulis Collected in the Republic of Congo

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Food and Nutrition Sciences 2024年第4期15卷 290-297页

作者： Josiane Enzonga Yoca jean paul Latran Ossoko Yves Okandza Michel Didace Mvoula Tsieri Laboratoire de Contrô le et Qualit&#233 des Aliments de l&#8217Ecole Nationale Sup&#233rieure d&#8217Agronomie et de Foresterie Universit&#233 Marien NGOUABI Brazzaville Congo Université Denis Sassou NGUESSO Brazzaville Congo

Coula edulis is non-timber forest product (NTFP) used in Africa for its hazelnuts, which contain edible seeds with a demonstrated nutritional potential. However, there have been very few scientific studies of this species in the Republic of Congo. Thus, the aim of the current study was therefore to determine the fatty acid composition of the oil extracted from Coula edulis hazelnut seeds collected at random in the Republic of Congo. The oil was extracted using the Soxhlet method and its fatty acid composition was determined by gas chromatography. The extracted oil from Coula edulis hazelnut kernels is rich in monounsaturated fatty acids (95.28%), particularly oleic acid (94.5%), which classifies it as an oleic oil and gives it interesting nutritional and therapeutic properties. On the other hand, saturated fatty acids (4.15%) and polyunsaturated fatty acids (0.35%) are not well represented. Its low poly-unsaturated fatty acid content makes it more stable when stored at room temperature.

关键词： Coula edulis Fatty Acids Oil Seeds Hazelnuts Republic of Congo NTFP

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Uncovering the Challenges of Faecal Sludge Management in Benin’s Urban Hubs: The Cases of Abomey-Calavi and Natitingou

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Journal of Environmental Protection 2024年第7期15卷 767-780页

作者： Nikita Topanou Blaise Agbatchi Gouvidé jean Gbaguidi Jacques Fatombi Fidèle paul Tchobo Kaba Laboratory of Chemical Research and Application (LaKReCA) Department of Chemistry Faculty of Science and Technology of Natitingou University of Abomey Natitingou Bnin Laboratory of Study and Research in Applied Chemistry Polytechnic School of Abomey-Calavi University of Abomey-Calavi (LERCA/EPAC/UAC) Cotonou Bnin

The issue of faecal sludge management in developing countries remains a significant challenge due to the indiscriminate discharge of sludge into the environment. This practice generates more environmental problems than it solves. This study aims to assess the management of household faecal sludge in the communes of Abomey-Calavi and Natitingou, Benin, in order to optimise the best storage conditions for subsequent more effective treatment. To this end, a sociological survey was conducted among households in Abomey-Calavi and Natitingou, as well as among manual emptiers. The results of our studies revealed that two types of latrines are used in households. 20.55% of households use traditional latrines, while 59.83% use ordinary latrines. 7.97% of households use both types of latrine. Moreover, the depth of the latrines varies from 2.5 m to 7 m in Abomey-Calavi and from 2.5 m to 8 m in Natitingou. Among households with a latrine, 28.26% empty their pit at least once, while 71.74% have never emptied it. The emptying cost varies between 35,000 FCFA and 90,000 FCFA. The mixing of faecal sludge with solid waste, including plastic materials, makes emptying difficult due to the obstruction of the vacuum pump. The results obtained will serve as a reliable database to facilitate decision-making in the context of faecal sludge management.

关键词： Management Faecal Sludge Valorization Benin

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Dosage Formula in Anesthesia for Manual Intrauterine Aspiration Post-Abortion in Resource-Limited Settings

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Pharmacology & Pharmacy 2024年第4期15卷 71-80页

作者： Serge Ibula Rivain Iteke Berthe Barhayiga Doddie Tsansya Roger Cishugi jean Jacques Kalongo Sandra Sagboze jean paul Cikwanine Dieudonné Sengeyi ISTM/Bukavu Anesthesia-Resuscitation Section CIMAC Hospital Center in Goma Goma The Democratic Republic of the Congo Department of Anesthesia-Resuscitation University of Lubumbashi Lubumbashi The Democratic Republic of the Congo Department of Anesthesia-Resuscitation University of Kinshasa Kinshasa The Democratic Republic of the Congo Department of Gyneco-Obstetrics University of Kinshasa Kinshasa The Democratic Republic of the Congo Anesthesia-Resuscitation Department Panzi Hospital Bukavu The Democratic Republic of the Congo

Setting: Provincial General Reference Hospital of Bukavu, General Reference Hospital of Panzi, General Reference Hospital of Ciriri, General Reference Hospital of Nyatende and Biopharm Hospital Center. Objective: Contribute to the improvement of the anesthetic ECP of patients benefiting from MVA for incomplete abortion, Describe the methodology used for adoption of the dosage formula in Anesthesia for MVA, present the mathematical demonstration leading to the dosage formula in anesthesia for MVA. Materials and Methods: Our study was descriptive by mathematical demonstration of obtaining the equilibrium constant of the dosage formula of bipuvacaine 0.1% and Fentanyl 50 µg% adapted to the weight and specific size of patients treated anesthetically in MVA cases for incomplete abortion. We also carried out an operational research by first determining the interval where our equilibrium constant is included and secondly by means of the ends of the intervals found correlated to the ends of intervals of possible weights and sizes in the being normal human female of childbearing age to arrive at the real numerical value of the equilibrium constant of the BUKAVU Dosage Formula in the case of anesthesia for MVA during the management of incomplete abortions. Results: TWO-STAGE OPERATIONAL RESEARCH: • Determination of the interval where the equilibrium constant x = −0.95 x x by crossing the means between the extremes of volumes of anesthetic drugs giving a satisfactory sensory block without hemodynamic disturbance and the extremes of normal weight and height for women of childbearing age. We ended up with X = 0.37. Conclusion: At the end of our study which had the general objectives of contributing to the improvement of the anesthetic PEC of patients receiving MVA for incomplete abortion and specific objectives of describing the methodology used for adoption of the dosage formula in Anesthesia for MVA and present the mathematical demonstration which resulted in the dosage formul

关键词： Dosage Anesthesia Intrauterine Aspiration DR Congo

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Pleomorphic leiomyosarcoma of the maxilla with metastasis to the colon:A case report

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World Journal of Gastrointestinal Endoscopy 2024年第6期16卷 361-367页

作者： Asma Alnajjar Abdulrahman Alfadda Abdullah Mohammad Alqaraawi Bader Alajlan jean paul Atallah Hussah Fahad AlHussaini Section of Gastroenterology Department of MedicineKing Faisal Specialist Hospital&Research CentreRiyadh 11211Saudi Arabia Department of Oncology King Faisal Specialist Hospital&Research CentreRiyadh 11211Saudi Arabia Department of Pathology King Faisal Specialist Hospital&Research CentreRiyadh 11211Saudi Arabia

BACKGROUND Pleomorphic leiomyosarcomas make up around 8.6%of all *** behave aggressively and often have poor *** can affect the gastrointestinal tract and *** date,pleomorphic leiomyosarcoma involving the mesocolon have been reported in nine *** SUMMARY The patient was a 44-year-old man with a history of pleomorphic leiomyosarcoma of the left maxilla with metastasis to the lung and *** most recent positron emission tomography-computed tomography(PET-CT)scan showed uptake in the ascending and transverse colons.A colonoscopy revealed a 5.0 cm×3.5 cm×3.0 cm pedunculated polyp in the ascending *** polyp was removed using hot snare polypectomy technique and retrieved with *** examination of the polyp showed a metastatic pleomorphic *** Uptake(s)on PET-CT in a patient with pleomorphic leiomyosarcoma should raise suspicion for metastasis.

关键词： Pleomorphic leiomyosarcoma Colon polyp Hot snare polypectomy Maxillary Spindle cell tumor Case report

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Relationship between Inter Canine Width and Inter Alar Width, Inter Commissural Width, and Inter Canthal Distance: A Pilot Study

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Open Journal of Stomatology 2024年第6期14卷 279-290页

作者： Grevisse Bindele Ndombolo Augustin Mantshumba Milolo Fidele Nyimi Bushabu Em Kalala Kazadi Pierrot Kumpanya Ntumba jean Marie Kayembe Bukama Elias Lubadi Tshilumba jean paul Sekele Isouradi Department of Prosthodontics Teaching Hospital of Kinshasa University Kinshasa Democratic Republic of the Congo National Center of Research in Dental Sciences Kinshasa Democratic Republic of the Congo Department of Oral and Maxillofacial Surgery Teaching Hospital of Kinshasa University Kinshasa Democratic Republic of the Congo Department of Periodontology Teaching Hospital of Kinshasa University Kinshasa Democratic Republic of the Congo Department of Operative Dentistry Teaching Hospital of Kinshasa University Kinshasa Democratic Republic of the Congo

The aim of this study was to determine the relationship between inter canine width (ICW) and inter alar width (IAW), inter commissural width (ICoW), and inter canthal distance (ICaD) in Bantu population. Materials and Methods: It was a pilot study included all participants who were aged at least 18 years, without a history of orthodontics treatment from Prosthodontics Department, Faculty of Dental Medicine, University of Kinshasa. The Ethics committee of the National Center of Research for Dental Sciences in DR Congo approved the study protocol (CNRSB 1504.218). The participants medical records were obtained from the interview and dental examination. Nature of the study was explained with participant information sheet, and an informed consent of all participants was obtained. The inclusion criteria were no missing maxillary and mandibular teeth, no diastema, and no anterior restorations, 18 years old before enrollment in the study. The exclusion criteria were inability to meet the above requirements, pregnancy, and refusal to participate in the study. The inter-canine width (ICW), inter alar width (IAW), inter canthal distance (ICaD) and inter-commissural width (ICoW) of each participant were measured with a digital Vernier caliper (Mitutoyo, UK Ltd.,) (0.01 mm) three times and the average value was recorded. The inter canthal distance (ICaD) was measured without applying pressure by bringing the recording parts of the caliper just in contact with the medial angle. The inter alar width (IAW) was marked with a fine marked pencil on the widest outer surface of the alae of the nose on either side or width. Those two points were measured without applying pressure by bringing the recording parts of the caliper just in contact with the outer surface. The participant was told to stop breathing shortly to avoid any change in shape of the nose. The inter-commissural width (ICoW) was determined by measuring the maxillary lip vermilion from commissure to commissure without the

关键词： Relationship Inter-Canine Width Inter Alar Width Inter Canthal Distance Inter Commissural Width

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