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Progress in Natural Science:Materials International 2006年第12期16卷 1295-1299页

作者： jean-paul vincken Luc C.J.M. SUURS Richard G.F. VISSER Laboratory of Plant Breeding Department of Plant Sciences Wageningen University Wageningen The Netherlands

Microbial starch-binding domains (SBD) and granule-bound starch synthase I (GBSSI) are proteins which are accumulated in potato starch granules. The efficiency of SBD and GBSSI for targeting active luciferase reporter proteins to granules during starch biosynthesis was compared. GBSSI or SBD sequences were fused to the N- or C-terminus of the luciferase (LUC) gene, via an artificial Pro-Thr encoding linker sequence. The genes were introduced into an amylose-free (amf) potato mutant. It appeared that SBD was superior to GBSSI as a targeting sequence, mainly because the luciferase retained higher activity in the SBD-containing fusion proteins than in the GBSSI-containing ones.

关键词： luciferase GBSS I SBD amf potato.

SBD在重组蛋白质中位移对其酶活性及与淀粉粒结合性能的影响

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南京师大学报（自然科学版） 2005年第1期28卷 88-92页

作者：季勤 jean-paul vincken Krit Raemakers Lucas Carolus Josephus Maria Suurs Richard Gerardus Franciscus Visser 瓦赫宁根大学植物科学系作物育种实验室

　将环状糊精糖基转移酶的SBD基因编码序列连接到萤光素酶基因的 5′ 端(SBD LUC)后,重组基因通过农杆菌介导导入马铃薯植株中.对重组蛋白在转基因淀粉粒中的定位与积累、重组蛋白中SBD与淀粉的亲和性以及萤光素酶的活性进行了分析比较... 详细信息

　将环状糊精糖基转移酶的SBD基因编码序列连接到萤光素酶基因的 5′ 端(SBD LUC)后,重组基因通过农杆菌介导导入马铃薯植株中.对重组蛋白在转基因淀粉粒中的定位与积累、重组蛋白中SBD与淀粉的亲和性以及萤光素酶的活性进行了分析比较.结果表明: 重组蛋白在马铃薯淀粉粒中获得了特异性表达, 当C 端SBD移位到重组萤光素酶蛋白的N 端后, SBD和淀粉的亲和性与LUC SBD重组蛋白质比较有所下降, 但萤光素酶活性则与马铃薯遗传背景有关.

关键词：重组蛋白质萤光素酶淀粉结合功能域转基因马铃薯

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利用GBSSI和SBD作为“锚”将荧光素酶定位到淀粉粒中效果的比较

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自然科学进展 2006年第6期16卷 679-683页

作者：季勤 jean-paul vincken Krit Raemakers Luc C.J.M.Suurs Richard G.F.Visser 淮阴师范学院生物系淮安223300 Laboratory of Plant Breeding Department of Plant SciencesWageningen UniversityWageningenThe Netherlands

利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(GBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUC)定位到淀粉粒中．并对GBSSI／LUC重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中GBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了... 详细信息

利用马铃薯淀粉粒结合型淀粉合成酶Ⅰ(GBSSI)作为“锚”,在马铃薯淀粉粒形成时将荧光素酶(LUC)定位到淀粉粒中．并对GBSSI／LUC重组蛋白质在转基因淀粉粒中的定位与积累、重组蛋白质中GBSSI与淀粉粒的亲和性以及荧光素酶的活性等进行了分析．结果表明,GBSSI／ LUC重组蛋白质在马铃薯淀粉粒中获得了特异性表达,但与以微生物的淀粉粒结合结构域(SBD) 作为“锚”的SBD／LUC重组蛋白质相比较,它与淀粉粒的亲和性以及荧光素酶的活性均下降．由此认为,在淀粉粒形成过程中,利用SBD作为“锚”将荧光素酶蛋白质定位到淀粉粒中的效果要优于GBSSI．

关键词：荧光素酶 GBSSI SBD amf马铃著

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A Global Group of Friends

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Beijing Review 2024年第29期67卷 26-27页

作者： jean paul Vargas the Sustainable Development Innovation Laboratory Central American Institute of Public Administration the Center for Historical Research of Central America University of Costa Rica

China's Global Development Initiative(GDI)is emerging as a transformative force in the international *** President Xi Jinping first publicized the conceplal the United Nations General Assembly in September *** its announcemen,the GDT's main components and objectives,aimed at fostering cunnectily and coperation between coninents,hawe atractod workdwide atentin.

关键词： Global foster objectives

Virtual brain twins: from basic neuroscience to clinical use

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National Science Review 2024年第5期11卷 72-86页

作者： Huifang E.Wang paul Triebkorn Martin Breyton Borana Dollomaja jean-Didier Lemarechal Spase Petkoski Pierpaolo Sorrentino Damien Depannemaecker Meysam Hashemi Viktor K.Jirsa Aix Marseille Université Institut National de la Santéet de la Recherche Médicale Institut de Neurosciences des Systèmes(INS) UMR1106 Service de Pharmacologie Clinique et Pharmacosurveillance AP–HM

Virtual brain twins are personalized, generative and adaptive brain models based on data from an individual's brain for scientific and clinical use. After a description of the key elements of virtual brain twins,we present the standard model for personalized whole-brain network models. The personalization is accomplished using a subject's brain imaging data by three means:(1) assemble cortical and subcortical areas in the subject-specific brain space;(2) directly map connectivity into the brain models, which can be generalized to other parameters; and(3) estimate relevant parameters through model inversion, typically using probabilistic machine learning. We present the use of personalized whole-brain network models in healthy ageing and five clinical diseases: epilepsy, Alzheimer's disease, multiple sclerosis, Parkinson's disease and psychiatric disorders. Specifically, we introduce spatial masks for relevant parameters and demonstrate their use based on the physiological and pathophysiological hypotheses. Finally, we pinpoint the key challenges and future directions.

关键词： virtual brain twin personalized modeling inference neuroscience brain disorder

Fuzzy Henstock-Kurzweil Triple Integral on a Type 1 Quasi-Fuzzy Parallelepipedal Domain

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Journal of Applied Mathematics and Physics 2024年第3期12卷 897-911页

作者： Kumwimba Seya Didier Kasongo Ntambwe Dany Panga Lutanda Grégoire Mayuke Katshongo jean paul Lunda Ngoy jean Pierre Département de Mathématiques et Informatique Faculté des Sciences Université de Lubumbashi Lubumbashi Democratic Republic of the Congo

In this article, we propose by using the Hausdorff distance Simpson’s rule for the triple integral of a fuzzy-valued function and the error bound of this method, one of the variables of which is fuzzy. In addition, thin δ-fine partitions are introduced. The integration domain is a quasi-fuzzy parallelipiped. A numerical example is presented in order to show the application and the significance of the method.

关键词： Fuzzy-Valued Function Hausdorff Distance Quasi-Fuzzy Parallelipiped Henstock Integral

Fatty Acid Composition of Hazelnut Kernel Oil from Coula edulis Collected in the Republic of Congo

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Food and Nutrition Sciences 2024年第4期15卷 290-297页

作者： Josiane Enzonga Yoca jean paul Latran Ossoko Yves Okandza Michel Didace Mvoula Tsieri Laboratoire de Contrô le et Qualit&#233 des Aliments de l&#8217Ecole Nationale Sup&#233rieure d&#8217Agronomie et de Foresterie Universit&#233 Marien NGOUABI Brazzaville Congo Université Denis Sassou NGUESSO Brazzaville Congo

Coula edulis is non-timber forest product (NTFP) used in Africa for its hazelnuts, which contain edible seeds with a demonstrated nutritional potential. However, there have been very few scientific studies of this species in the Republic of Congo. Thus, the aim of the current study was therefore to determine the fatty acid composition of the oil extracted from Coula edulis hazelnut seeds collected at random in the Republic of Congo. The oil was extracted using the Soxhlet method and its fatty acid composition was determined by gas chromatography. The extracted oil from Coula edulis hazelnut kernels is rich in monounsaturated fatty acids (95.28%), particularly oleic acid (94.5%), which classifies it as an oleic oil and gives it interesting nutritional and therapeutic properties. On the other hand, saturated fatty acids (4.15%) and polyunsaturated fatty acids (0.35%) are not well represented. Its low poly-unsaturated fatty acid content makes it more stable when stored at room temperature.

关键词： Coula edulis Fatty Acids Oil Seeds Hazelnuts Republic of Congo NTFP

Uncovering the Challenges of Faecal Sludge Management in Benin’s Urban Hubs: The Cases of Abomey-Calavi and Natitingou

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Journal of Environmental Protection 2024年第7期15卷 767-780页

作者： Nikita Topanou Blaise Agbatchi Gouvidé jean Gbaguidi Jacques Fatombi Fidèle paul Tchobo Kaba Laboratory of Chemical Research and Application (LaKReCA) Department of Chemistry Faculty of Science and Technology of Natitingou University of Abomey Natitingou Bnin Laboratory of Study and Research in Applied Chemistry Polytechnic School of Abomey-Calavi University of Abomey-Calavi (LERCA/EPAC/UAC) Cotonou Bnin

The issue of faecal sludge management in developing countries remains a significant challenge due to the indiscriminate discharge of sludge into the environment. This practice generates more environmental problems than it solves. This study aims to assess the management of household faecal sludge in the communes of Abomey-Calavi and Natitingou, Benin, in order to optimise the best storage conditions for subsequent more effective treatment. To this end, a sociological survey was conducted among households in Abomey-Calavi and Natitingou, as well as among manual emptiers. The results of our studies revealed that two types of latrines are used in households. 20.55% of households use traditional latrines, while 59.83% use ordinary latrines. 7.97% of households use both types of latrine. Moreover, the depth of the latrines varies from 2.5 m to 7 m in Abomey-Calavi and from 2.5 m to 8 m in Natitingou. Among households with a latrine, 28.26% empty their pit at least once, while 71.74% have never emptied it. The emptying cost varies between 35,000 FCFA and 90,000 FCFA. The mixing of faecal sludge with solid waste, including plastic materials, makes emptying difficult due to the obstruction of the vacuum pump. The results obtained will serve as a reliable database to facilitate decision-making in the context of faecal sludge management.

关键词： Management Faecal Sludge Valorization Benin

Dosage Formula in Anesthesia for Manual Intrauterine Aspiration Post-Abortion in Resource-Limited Settings

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Pharmacology & Pharmacy 2024年第4期15卷 71-80页

作者： Serge Ibula Rivain Iteke Berthe Barhayiga Doddie Tsansya Roger Cishugi jean Jacques Kalongo Sandra Sagboze jean paul Cikwanine Dieudonné Sengeyi ISTM/Bukavu Anesthesia-Resuscitation Section CIMAC Hospital Center in Goma Goma The Democratic Republic of the Congo Department of Anesthesia-Resuscitation University of Lubumbashi Lubumbashi The Democratic Republic of the Congo Department of Anesthesia-Resuscitation University of Kinshasa Kinshasa The Democratic Republic of the Congo Department of Gyneco-Obstetrics University of Kinshasa Kinshasa The Democratic Republic of the Congo Anesthesia-Resuscitation Department Panzi Hospital Bukavu The Democratic Republic of the Congo

Setting: Provincial General Reference Hospital of Bukavu, General Reference Hospital of Panzi, General Reference Hospital of Ciriri, General Reference Hospital of Nyatende and Biopharm Hospital Center. Objective: Contribute to the improvement of the anesthetic ECP of patients benefiting from MVA for incomplete abortion, Describe the methodology used for adoption of the dosage formula in Anesthesia for MVA, present the mathematical demonstration leading to the dosage formula in anesthesia for MVA. Materials and Methods: Our study was descriptive by mathematical demonstration of obtaining the equilibrium constant of the dosage formula of bipuvacaine 0.1% and Fentanyl 50 µg% adapted to the weight and specific size of patients treated anesthetically in MVA cases for incomplete abortion. We also carried out an operational research by first determining the interval where our equilibrium constant is included and secondly by means of the ends of the intervals found correlated to the ends of intervals of possible weights and sizes in the being normal human female of childbearing age to arrive at the real numerical value of the equilibrium constant of the BUKAVU Dosage Formula in the case of anesthesia for MVA during the management of incomplete abortions. Results: TWO-STAGE OPERATIONAL RESEARCH: • Determination of the interval where the equilibrium constant x = −0.95 x x by crossing the means between the extremes of volumes of anesthetic drugs giving a satisfactory sensory block without hemodynamic disturbance and the extremes of normal weight and height for women of childbearing age. We ended up with X = 0.37. Conclusion: At the end of our study which had the general objectives of contributing to the improvement of the anesthetic PEC of patients receiving MVA for incomplete abortion and specific objectives of describing the methodology used for adoption of the dosage formula in Anesthesia for MVA and present the mathematical demonstration which resulted in the dosage formul

关键词： Dosage Anesthesia Intrauterine Aspiration DR Congo

Experimental Assessment of the Thermal Performance of Two Corrugated Metal Roofs

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Open Journal of Applied Sciences 2024年第9期14卷 2374-2392页

作者： Kouamé jean-Louis Kouakou Ekoun paul Magloire Koffi Bi Tra Désiré Zinla Prosper Gbaha Laboratoire des Procds Industriels de Synthse de lEnvironnement et des Energies Nouvelles Institut National Polytechnique Felix Houphout-Boigny Yamoussoukro Cte dIvoire

This experimental study is a contribution to the search for solutions to reduce indoor heat gain through sheet metal roofing in hot weather. It has evaluated the thermal impact of two different sheet metal roofs inside of two identical test buildings in sunny weather and cloudy weather conditions. Test building 1 has a single sheet corrugated roof and the building 2 is covered with roof made from top to bottom with corrugated sheet metal, a 12 mm thick serpentine copper tube in which water is circulated, a sheet of aluminium foil acting as a heat reflector, a 4 cm thick polystyrene panel and a 1.5 cm thick plywood. A maximum reduction of 15.1˚C in the temperature of the inner face of the test Building 2 roof was obtained comparatively to the temperature of the inner face of the test Building 1 roof consisting of a single sheet of metal at the warmest hours. In addition, the simple corrugated sheet metal roof of the test building generates high and varied temperatures inside the building. Whereas the proposed heat recovery roof favours low and relatively uniform temperatures inside the building. The proposed sheet metal roof construction technique is very effective in reducing the heat gain through the roof considerably;thus improving the thermal comfort inside sheet metal roofed dwellings. Hot water has been produced by recovering heat from the metal sheet of the roof of test building 2. The temperature of the hot water produced reached of 39˚C. This study could be also an alternative for the reduction of energy consumption due to the use of mechanical means for cooling of sheet metal roofed houses and the reduction of the use of fossil fuels for domestic hot water production.

关键词： Metal Roof Corrugated Time Lag Decrement Factor