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The Difference in Calcium Levels in Aspergillus nidulans Grown on Glucose or Pectin

Advances in Microbiology 2012年第2期2卷 117-121页

作者： janice aparecida rafael Suraia Said Departamento de Ciencias Farmaceuticas Faculdade de Ciencias Farmaceuticas de Ribeirao PretoUniversidade de Sao PauloSao PauloBrazil

Understanding the growth regulatory mechanisms in filamentous fungi is very important for the production of medicines for antifungal therapies. It is well established that Ca2+ gradient is essential for hyphal growth and that one mechanism responsible for the Ca2+ cellular concentration starts with the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) by receptor-regulated forms of phosphoinositide-specific phospholipase C (PI-PLC). In the present study the levels of calcium in Aspergillus nidulans wild type (A26) and plcA-deficient mutant (AP27) growing in a carbon source readily assimilated, as glucose or pectin a non-readily assimilated carbon source was investigated. Intracellular calcium levels in A26 were higher in the presence of glucose than in pectin, but lower in AP27 independently of the carbon source and in AP27 the vesicular calcium distribution occurred mainly at the apex of the hyphae. Delay in nuclear division was also observed if A26 and AP27 were grown in pectin presence when compared with growth in glucose. For the first time, it is demonstrated that the levels of intracellular Ca2+ were higher when A. nidulans was growing in glucose than in a non readily assimilated carbon source as pectin. Further, it also showed that the plcA gene, although not essential, may be responsible for high-molecular weight carbon source recongnation, for the intracellular Ca2+ levels maintenance and consequently by the nuclear division in A. nidulans.

关键词： Aspergillus nidulans Ca^(2+) Signaling Phospholipase C

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Perinatal transmission of dengue virus in Puerto Rico: a case report

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Open Journal of Obstetrics and Gynecology 2011年第3期1卷 90-93页

作者： janice Pérez-Padilla rafael Rosario-Casablanca Luis Pérez-Cruz Carmen Rivera-Dipini Kay Marie Tomashek Dengue Branch Division of Vector-Borne Diseases Centers For Disease Control and Prevention San Juan Puerto Rico. HIMA San Pablo Hospital Fajardo Puerto Rico.

We report a laboratory confirmed case of vertical transmission of dengue in a mother-child pair in the eastern part of Puerto Rico. The clinical course of the pregnant female suggested a GBS infection, but laboratory tests confirmed it was dengue infection, one week after delivery. The male infant was healthy at birth, but one week after birth developed clinical complications related to vertical transmission of dengue. This report targets physicians in dengue endemic countries like Puerto Rico to be aware of the possibility of vertical transmission of dengue in symptomatic pregnant patients, especially around the time of delivery.

关键词： Dengue Perinatal Transmission Bloodborne

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Hyphal Morphology and Elongation Alterations in Aspergillus nidulans Provoked by the Diterpene Kaurenoic Acid

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Advances in Microbiology 2013年第5期3卷 438-441页

作者： janice A.rafael Nilton S.Arakawa Sérgio R.Ambrosio Fernando B.Da Costa Suraia Said Faculdade de Ciências Farmacêuticas de Ribeirao Preto Universidade de Sao PauloRibeirao PretoBrazil Centro de Ciências da Saúde Universidade do Vale do ParaíbaSao Josédos CamposBrazil Departamento de Ciências Farmacêuticas Universidade Estadual de LondrinaLondrinaBrazil Laboratório de Biotransformacao Universidade de FrancaFrancaBrazil

Kaurenoic acid (KA), a kaurane-type diterpene extracted from leaves of Mikania hirsutissima, was previously reported as an inhibitor of vascular contractility mainly by blocking extracellular Ca2+ influx. The compound is known for several other biological activities such as antiparasitic, antispasmodic and antibacterial activity. The aim of the present study was to investigate the effect of KA on Aspergillus nidulans. KA (0.3 mM) showed fungistatic activity against A. nidulans with visible hyphal elongation and morphology damage. These effects were reverted by CaCl2 addition showing that KA interferes with intracellular Ca2+ gradient in A. nidulans. This is the first report on the mechanism of action of KA involving calcium levels by altering the elongation of fungi hyphae.

关键词： Aspergillus nidulans Calcium Gradient Fungistatic Activity Kaurenoic Acid

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