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规范护理人员血培养采集方法对预防血液标本污染的影响分析与对策

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中国卫生产业 2024年第7期21卷 12-14页

作者：周华章亿香蒋庭玲姚东海广西壮族自治区职业防治研究院(广西壮族自治区工人医院)医院感染管理科广西南宁530021

目的探讨规范护理人员采集血培养操作方法提高血培养检验结果的准确性效果。方法选取2022年1—6月广西壮族自治区工人医院的10名护理人员与其采集的患者血培养标本1900例作为对照组,采用常规操作流程。选取2023年1—6月本院10名护理人... 详细信息

目的探讨规范护理人员采集血培养操作方法提高血培养检验结果的准确性效果。方法选取2022年1—6月广西壮族自治区工人医院的10名护理人员与其采集的患者血培养标本1900例作为对照组,采用常规操作流程。选取2023年1—6月本院10名护理人员及其采集的患者血培养标本2992例作为观察组,采用规范化标本采集管理。两组护理人员为同一批。比较两组血培养标本污染发生情况、护理人员的工作情况评分。结果观察组总污染率(2.51%)低于对照组(4.63%),差异有统计学意义(χ^(2)=5.131,P<0.05)。观察组分离阳性率低于对照组,差异有统计学意义(P<0.05)。观察组护理人员工作情况评分高于对照组,差异有统计学意义(P均<0.05)。结论采用规范化标本采集方法管理护理人员,对于预防血液标本污染具有关键作用。

关键词：血培养标本采集方法对策

复方磺胺甲噁唑治疗百日咳的临床和实验室研究进展

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儿科药学杂志 2024年第9期30卷 51-55页

作者：翟心怡袁林王亚娟姚开虎北京市西城区展览路社区卫生服务中心北京100026 首都医科大学附属北京儿童医院北京100045 首都儿科研究所附属儿童医院北京100020

百日咳是百日咳鲍特菌(Bordetella pertussis,BP)引起的强传染性呼吸道疾病。长期以来,红霉素、克拉霉素和阿奇霉素等大环内酯类抗菌药物是百日咳抗菌治疗的首选药物。1994年从美国亚利桑那州的1例2月龄百日咳患儿分离了第一株红霉素耐... 详细信息

百日咳是百日咳鲍特菌(Bordetella pertussis,BP)引起的强传染性呼吸道疾病。长期以来,红霉素、克拉霉素和阿奇霉素等大环内酯类抗菌药物是百日咳抗菌治疗的首选药物。1994年从美国亚利桑那州的1例2月龄百日咳患儿分离了第一株红霉素耐药百日咳鲍特菌(erythromycin-resistance Bordetella pertussis,ERBP),此后欧美虽有零星病例报告,但没有发现ERBP流行的迹象[1]。2013年,国内研究者在山东2例无症状青少年的鼻咽拭子首次发现ERBP[1],随后北京、西安、深圳等地对临床分离菌株进行药物敏感性检测,发现ERBP在分离株中的占比为52.3%~91.1%,且ERBP最低抑菌浓度(minimum inhibitory concentration,MIC)均>256 mg/L[1-2]。

关键词：百日咳抗菌治疗临床分离菌株鼻咽拭子最低抑菌浓度病例报告无症状阿奇霉素

Ventricular tachycardia originating from the His bundle: A case report

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World Journal of Clinical Cases 2021年第32期9卷 10040-10045页

作者： Lu-yao Zhang shu-Juan Dong Hai-Jia Yu Ying-Jie Chu Department of Cardiology Zhengzhou University People’s HospitalZhengzhou 450003Henan ProvinceChina Department of Emergency Zhengzhou University People’s HospitalZhengzhou 450003Henan ProvinceChina

BACKGROUND Ventricular tachycardia(VT)commonly occurs among patients with heart failure and can even cause sudden cardiac *** originating from the His bundle branch has been rarely *** present the case of a patient with VT from the His bundle *** sUMMARY A 58-year-old female complained of paroxysmal palpitations and dizziness for approximately 6 *** had a history of fatty liver and cholecystitis,and carotid atherosclerosis could not be excluded from the ultrasound *** evaluation of the electrocardiogram obtained after admission showed spontaneous conversion between two different *** possible electrophysiologic mechanism suggested that the dual-source VT originated from the same source,the His bundle ***,the His bundle branch was ablated,and a dualchamber pacemaker was inserted into the patient’s *** further VT occurred during the 3-year follow-up after hospital *** The diagnosis of VT originating from the His bundle is rare and difficult to *** results of this study showed VT originating from the His bundle based on a careful evaluation of the electrocardiogram,and the diagnosis was confirmed by an intracardiac electrophysiologic examination.

关键词： Electrocardiogram His bundle Ventricular tachycardia Case report

Long noncoding RNA LINC01124 activates hepatocellular carcinoma cell proliferation, migration, and invasion by absorbing microRNA-1247-5p and overexpressing FOXO3

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Oncology Research 2021年第3期29卷 175-187页

作者： LEI sUN YUE ZHANG YUQIN yao HONGLIN DU YUEHUA ZHANG AIPING FANG Department of Geriatric Medicine West China School of Public Health and West China Fourth HospitalSichuan UniversityChengdu610041China state Key Laboratory of Biotherapy/Collaborative Innovation Center for Biotherapy West China HospitalSichuan UniversityChengdu610041China Department of Hepatobiliary and Pancreas surgery Second Clinical Medical College of Jinan University(Shenzhen People’s Hospital)ShenzhenChina Respiratory Disease Research Center West China School of Public Health and West China Fourth HospitalSichuan UniversityChengdu610041China

Long intergenic non-protein coding RNA 1124(LINC01124)has been identified as an important regulator of non-small-cell lung ***,the expression and detailed role of LINC01124 in hepatocellular carcinoma(HCC)remain unestablished to ***,this study aimed to elucidate the role of LINC01124 in the aggressiveness of HCC cells and identify the underlying regulatory *** reverse transcriptase-polymerase chain reaction was performed to measure the expression of LINC01124 in *** Counting Kit-8 assay,Transwell cell migration and invasion assays,and a xenograft tumor model were used to investigate the function of LINC01124 in HCC cells,and bioinformatics analysis,RNA immunoprecipitation,luciferase reporter assay,and rescue experiments were used to elucidate the underlying ***,LINC01124 overexpression was confirmed in HCC tissues as well as cell ***,the downregulation of LINC01124 decreased HCC cell proliferation,migration,and invasion in vitro,whereas the upregulation of LINC01124 triggered the opposite ***,LINC01124 ablation impaired tumor growth in *** analyses revealed that LINC01124 functions as a competing endogenous RNA to sponge microRNA-1247-5p(miR-1247-5p)in HCC ***,forkhead box O3(FOXO3)was identified as a direct target of ***3 was positively regulated by LINC01124 in HCC cells through the sequestration of ***,rescue assays revealed that the inhibition of miR-1247-5p or overexpression of FOXO3 reversed the effects of LINC01124 silencing on the HCC cell malignant *** summary,LINC01124 plays a tumor-promoting role in HCC by regulating the miR-1247-5p-FOXO3 *** LINC01124-miR-1247-5p-FOXO3 pathway may provide a foundation for the identification of alternative therapies for HCC.

关键词： HCC Competing endogenous RNA theory Anticancer treatments

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基于叶天士学术思想论治功能性腹泻

中国中西医结合消化杂志 2024年第8期32卷 727-729页

作者：夏津滨苏晓兰程遥刘登科北京中医医院顺义医院中医内科北京101300 中国中医科学院望京医院脾胃科功能性胃肠病中医诊治北京市重点实验室

功能性腹泻属中医“泄泻”范畴,西药治疗该病具有一定的局限性,因复发率较高,是目前亟待解决的难题。清代著名医家叶天士学验俱丰,其对“泄泻”的诊疗造诣颇深。叶氏在“泄泻”病案的诊治中论治精当,勤求古训,在实践中形成了鲜明的叶氏... 详细信息

功能性腹泻属中医“泄泻”范畴,西药治疗该病具有一定的局限性,因复发率较高,是目前亟待解决的难题。清代著名医家叶天士学验俱丰,其对“泄泻”的诊疗造诣颇深。叶氏在“泄泻”病案的诊治中论治精当,勤求古训,在实践中形成了鲜明的叶氏特色,且“泄泻”论治的思想对后世具有指导意义。中药治疗功能性腹泻药简力专、效若桴鼓,本文结合叶氏治疗“泄泻”疾病的学术思想,以期为临床医师有效诊疗功能性腹泻提供思路。

关键词：功能性腹泻治疗叶天士

A new estimate of global soil respiration from 1970 to 2008

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Chinese science Bulletin 2013年第33期58卷 4153-4160页

作者： CHEN shuTao HUANG yao XIE Wei ZOU JianWen LU YanYu HU ZhengHua school of Environmental science and Engineering Nanjing University of Information Science and Technology state Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of Sciences College of Resources and Environmental sciences Nanjing Agricultural University Anhui Climate Center school of Applied Meteorology Nanjing University of Information Science and Technology

土壤呼吸(R s ) 是在陆上的生态系统加重我们碳周期的理解的关键过程之一。在有在最高、最低的估计之间的 70 Pg C a1 的差别的以前的全球 R s 估计的大无常遗体。因此，现在的学习试图估计全球年度 R s 并且用包括了气候的因素(温度和... 详细信息

土壤呼吸(R s ) 是在陆上的生态系统加重我们碳周期的理解的关键过程之一。在有在最高、最低的估计之间的 70 Pg C a1 的差别的以前的全球 R s 估计的大无常遗体。因此，现在的学习试图估计全球年度 R s 并且用包括了气候的因素(温度和降水) 和表层土(020 厘米) 的一个半机械学的、以经验为主地基于的模型在全球年度 R s 调查 interannual 和空间可变性器官的碳存储。从 147 个测量地点的年度 R s 的大约 657 出版研究在这元分析被包括。气候，表面空气温度，和土壤性质上的从 1970 ～ 2008 的全球数据集合是镇定的。蒙特卡罗方法被用来宣传模拟错误到全球 R s。结果显示吝啬的年度全球 R s 是 94.4 Pg C a1，增加在粗略地从 1970 ～ 2008 的 0.04 Pg C a1 (0.04% a1 ) 。R s 率增加了从更冷，更干燥并且少些玷污充满碳的区域到更温暖、更潮湿、更充满碳的区域。最高的 R s 率出现在热带森林里，当最低的在极、荒芜的区域时。年度 R s 与全球温度异例直接相关，建议在温度的 interannual 可变性为在预言的全球 R s 的 interannual 变化负责。全球 R s 从高纬度的地区增加了到低纬度的地区。进一步的研究被建议探索在土壤呼吸和植被人物之间的关系。

关键词：土壤呼吸估计年际变化陆地生态系统蒙特卡罗方法温度距平气候因素资源丰富

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火龙果茎溃疡病植株茎组织细菌群落特征分析

南方农业学报 2024年第4期55卷 1023-1034页

作者：梁洲勇姚姿婷曹雪颖韦增亭赵渊朱桂宁邹承武广西大学农学院/植物科学国家级实验教学示范中心广西南宁530004 农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室/广西农业科学院植物保护研究所广西南宁530007 广西亚热带作物研究所广西南宁530001

【目的】比较火龙果健康植株与溃疡病植株茎组织的内生细菌群落特征和功能差异,为揭示火龙果溃疡病发生机制和研究防治技术提供理论依据。【方法】采集火龙果健康植株和溃疡病植株茎组织,利用Illumina Miseq测序平台对植株茎组织内生细... 详细信息

【目的】比较火龙果健康植株与溃疡病植株茎组织的内生细菌群落特征和功能差异,为揭示火龙果溃疡病发生机制和研究防治技术提供理论依据。【方法】采集火龙果健康植株和溃疡病植株茎组织,利用Illumina Miseq测序平台对植株茎组织内生细菌的16s rDNA序列进行测定,分析健康植株与罹病植株茎组织中的细菌群落特征差异。【结果】火龙果健康植株茎组织的细菌群落种类和扩增子序列变体(Amplicon sequence variant,AsV)数均低于罹病植株;健康植株与罹病植株茎组织的细菌群落多样性无显著差异(P>0.05)。在细菌优势门中,罹病植株茎组织的放线菌门(Actinobacteriota)、绿弯菌门(Chloroflexi)和异球菌门(Deinococcota)相对丰度较高,而健康植株茎组织的拟杆菌门(Bacteroidota)相对丰度较高。罹病植株茎组织的优势细菌属有27个,其中假单胞菌属(Pseudomonas)、芽孢杆菌属(Bacillus)、鞘氨醇单胞菌属(sphingomonas)、异球菌属(Deinococcus)、梭菌属(Clostridium_sensu_stricto_1)、短小杆菌属(Curtobacterium)、黄单胞菌属(Xanthomonas)、毛梭菌属(Lachnoclostridium)、微枝形杆菌属(Microvirga)、溶杆菌属(Lysobacter)、Klenkia和类芽孢杆菌属(Paenibacillus)相对丰度较高。健康植株茎组织的优势细菌属有26个,其中绒毛杆菌科未分类属(norank_f_Muribaculaceae)、拟杆菌属(Bacteroides)、乳杆菌属(Lactobacillus)、甲基杆菌属群(Methylobacterium-Methylorubrum)、黄单胞菌科未知属(unclassified_f_Xanthomonadaceae)、副杆菌属(Parabacteroides)、罗尔斯通氏菌属(Ralstonia)、红球菌属(Rhodococcus)、链霉菌属(streptomyces)、双歧杆菌属(Bifidobacterium)、放线杆菌属(Acinetobacter)和纤维弧菌属(Cellvibrio)的相对丰度较高。线性判别分析(LEfse)结果显示,健康植株茎组织具有较多指示菌群,而罹病植株茎组织的指示细菌属只有4个不同的属,分别是黄单胞菌属、短小杆菌属、毛梭菌属和新鞘氨醇单胞菌属(Novosphingobium)。细菌功能预测结果显示,溃疡病植株茎组织内生细菌在膜转运、碳水化合物代谢、其他氨基酸代谢和其他次级代谢产物的生物合成等4个二级功能层中的基因拷贝数显著高于健康植株茎(P<0.05)。【结论】火龙果发生茎溃疡病后其茎组织细菌群落多样性无明显改变,但部分优势菌属的比例发生明显改变,其中4个指示细菌属可能与溃疡病发生有关。

关键词：火龙果溃疡病茎组织细菌群落多样性

Early-stage mineralization of hydrothermal tubeworms: New insights into the role of microorganisms in the process of mineralization

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Chinese science Bulletin 2008年第2期53卷 251-261页

作者： PENG XiaoTong ZHOU HuaiYang TANG song yao HuiQiang JIANG Lei WU Zidun Guangzhou Institute of Geochemistry Chinese Academy of Sciences Guangzhou 510640 China National Key Lab of Marine Geology Tongji University Shanghai 200092 China

As an important part of marine hydrothermal system, hydrothermal vent faunas live in hydrothermal inorganic environment and closely interact with hydrothermal inorganic environment. sometimes, they can participate in the mineralization process of modern hydrothermal site. Hydrothermal vent faunas, particularly vestimentiferan and polychaete tubeworms, are occasionally preserved in the geological record. study on the early mineralization process of hydrothermal vent fauna is significant for under- standing the interaction between mineral and organism, and also the formation and preservation mechanism of geological fossil in hydrothermal environment. In this paper, the early stage of miner- alization of Vestimentiferan Ridgeia piscesae tubes collected from Juan de Fuca Ridge is studied. The results showed that a lot of filamentous microorganisms were unevenly distributed on the surface of internal wall and in the interspace of the wall of tubeworm. In some cases, microorganisms aggregated as thin layers in or on the wall of tubeworm. The surfaces of microbial cells and the products of micro- bial degradation may play an important role in the early mineralization of tubeworm. semitransparent thin layers of organic matter containing sulfur and sulfur granules were commonly found on the wall of tubeworm with lower degree of mineralization. The degradation production of these semitransparent thin layers may accelerate the mineralization of tube wall during the early stage. EDs results showed that on the tube walls some chemical elements such as Fe, P, Ca and si are selectively enriched from ambient hydrothermal environment. Interestingly, P, Ca and si covary with Fe content. Because element s originated from the bio-oxidation of H2s by symbiotic microorganism in the tissue of tubeworm, it can be considered as a biomarker when studying the mineralization process of tube wall. Based on the characteristics of tubeworms with different degrees of mineralization, we suggested t

关键词：生物矿化热水通气口微生物无机环境

New Type Austenite Dynamic Recrystallization of Microalloyed Forging steels 38MnVs During Forging Process

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Journal of Iron and steel Research International 2013年第5期20卷 57-60页

作者： ZHANG Tong-bo sHEN Yong LIU Pan YONG Qi-long BAO yao-zong Xinxing Pipes Group Co. Ltd. Great Wall Motor Co. Ltd. Central Iron and steel Research Institute

Hot compression tests of microalloyed forging steels 38MnVs were carried out on the Gleeble-3800 thermo-mechanical simulator at the deformation temperatures from 950 to 1 150 ℃ with the strain rates ranging from 0.1 to 10 s^（-1）. The effects of the deformation temperature and strain rate on the austenite dynamic recrystallization and microstructural changes were researched. The experimental results show that the dynamic recrystallization accelerated with the increase of the deformation temperatures and the decrease of the strain rate. The activation energy of dynamic recrystallization was calculated, which was about 275.453 kJ/mol. The relation between the dynamic recrystallization and the Z-parameter was investigated, and the state chart of the dynamic recrystallization of the microalloyed forging steel 38MnVs was made according to the experimental data and the deformation parameters.

关键词： microalloyed forging steel activation energy dynamic recrystallization austenite grain size state chart 38MnVs