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Impact of post-ruminally infused macronutrients on bovine mammary gland expression of genes involved in fatty acid synthesis, energy metabolism, and protein synthesis measured in RNA isolated from milk fat

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Journal of Animal Science and Biotechnology 2020年第4期11卷 1220-1231页

作者： Kelly Nichols AndréBannink Jurgen van Baal jan dijkstra Animal Nutrition Group Wageningen University and ResearchPO Box 3386700AHWageningenthe Netherlands Wageningen Livestock Research Wageningen University and ResearchPO Box 3386700AHWageningenthe Netherlands

Background: Characterising the regulation of milk component synthesis in response to macronutrient supply is critical for understanding the implications of nutritional interventions on milk production. Gene expression in mammary gland secretory cells was measured using RNA isolated from milk fat globules from 6 Holstein-Friesian cows receiving 5-d abomasal infusions of saline, essential amino acids(AA), or glucose(GG) or palm olein(LG)without(LAA) or with(HAA) essential AA, according to a 6 × 6 Latin square design. RNA was isolated from milk fat samples collected on d 5 of infusion and subjected to real-time quantitative PCR. We hypothesised that m RNA expression of genes involved in de novo milk fatty acid(FA) synthesis would be differently affected by GG and LG,and that expression of genes regulating transfer of tricarboxylic acid cycle intermediates would increase at the HAA level. We also hypothesised that the HAA level would affect genes regulating endoplasmic reticulum(ER)homeostasis but would not affect genes related to the mechanistic target of rapamycin complex 1(m TORC1) or the integrated stress response(ISR) ***: Infusion of GG did not affect de novo milk FA yield but decreased expression of FA synthase(FASN).Infusion of LG decreased de novo FA yield and tended to decrease expression of acetyl-Co A carboxylase 1(ACC1).The HAA level increased both de novo FA yield and expression of ACC1, and tended to decrease expression of mitochondrial phosphoenolpyruvate carboxykinase(PCK2). m RNA expression of m TORC1 signaling participants was not affected by GG, LG, or AA level. Expression of the ε subunit of the ISR constituent eukaryotic translation initiation factor 2 B(EIF2 B5) tended to increase at the HAA level, but only in the presence of LG. X-box binding protein 1(XBP1) m RNA was activated in response to LG and the HAA ***: Results show that expression of genes involved in de novo FA synthesis responded to glucogenic,lipogenic, and

关键词： Cytoplasmic crescent Endoplasmic reticulum biogenesis Mammary cell Milk fat globule Milk synthesis Tricarboxylic acid cycle

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MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells

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Animal Nutrition 2022年第3期10卷 319-328页

作者： Fang Wang Jürgen van Baal Lu Ma Xuejun Gao jan dijkstra Dengpan Bu State Key Laboratory of Animal Nutrition Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing100193China Animal Nutrition Group Wageningen University and ResearchWageningen6708WDthe Netherlands College of Animal Science Yangtze UniversityJingzhou434020China Joint Laboratory on Integrated Crop-Tree-Livestock Systems of the Chinese Academy of Agricultural Sciences(CAAS) Ethiopian Institute of Agricultural Research(EIAR)and World Agroforestry Center(ICRAF)Beijing100193China

Myotonic dystrophy-related Cdc42-binding kinase alpha(MRCKα)is an integral component of signaling pathways controlling vital cellular processes,including cytoskeletal reorganization,cell proliferation and cell *** this study,we investigated the physiological role of MRCKα in milk protein and fat production in dairy cows,which requires a dynamic and strict organization of the cytoskeletal network in bovine mammary epithelial cells(BMEC).Within a selection of 9 Holstein cows,we found that both mRNA and protein expression of MRCKα in the mammary gland were upregulated during lactation and correlated positively(r>0.89)with the mRNA and protein levels of *** positive correlations(r>0.79)were found in a primary culture of BMEC stimulated with prolactin for 24 *** these cells,silencing of MRCKα decreased basal b-casein,sterol-regulatory element binding protein(SREBP)-1 and cyclin D1 protein level,phosphorylation of mTOR,triglyceride secretion,cell number and viabilitydwhile overexpression of MRCKα displayed the reversed ***,silencing of MRCKα completely prevented the stimulatory action of prolactin on the same *** data demonstrate that MRCKα is a critical mediator of prolactin-induced lactogenesis via stimulation of the mTOR/SREBP1/cyclin D1 signaling pathway.

关键词： Myotonic dystrophy-related Cdc42-binding kinase alpha Bovine mammary gland Prolactin Mechanistic target of rapamycin Sterol regulatory element-binding protein-1 Cyclin D1

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Performance of the Montreal classification for inflammatory bowel diseases

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World Journal of Gastroenterology 2014年第41期20卷 15374-15381页

作者： Lieke M Spekhorst Marijn C Visschedijk Rudi Alberts Eleonora A Festen Egbert-jan van der Wouden Gerard dijkstra Rinse K Weersma Department of Gastroenterology and Hepatology University of Groningen and University Medical Centre Groningen Department of Genetics University of Groningen and University Medical Centre Groningen Department of Gastroenterology Isala Clinics Zwolle

AIM: To validate the Montreal classification system for Crohn’s disease (CD) and ulcerative colitis (UC) within the Netherlands.

关键词： Crohn’ s disease Ulcerative colitis Montreal classification Phenotypes- inter-observer agreement

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快速原位形成的生物水凝胶的合成及蛋白质释放

快速原位形成的生物水凝胶的合成及蛋白质释放

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2007年全国高分子学术论文报告会

作者：钟志远 Christine Hiemstra Rong Jin Pieter J.dijkstra jan Feijen 苏州大学化学化工学院生物医用高分子材料实验室 Department of Polymer Chemistry and Biomaterials Institute for Biomedical Technology Faculty of Science and TechnologyUniversity of TwenteP.O.Box 2177500 AE Enschedethe Netherlands. Department of Polymer Chemistry and Biomaterials Institute for Biomedical Technology Faculty of Science and TechnologyUniversity of TwenteP.O.Box 2177500 AE Enschedethe Netherlands. Department of Polymer Chemistry and Biomaterials Institute for Biomedical Technology Faculty of Science and TechnologyUniversity of TwenteP.O.Box 2177500 AE Enschedethe Netherlands. Department of Polymer Chemistry and Biomaterials Institute for Biomedical Technology Faculty of Science and TechnologyUniversity of TwenteP.O.Box 2177500 AE Enschedethe Netherlands.

＜正＞水凝胶因良好的生物相容性和渗透性等在生物医学领域如药物／蛋白质的缓释、生物组织工程等展现了广阔的应用前景。近年来,我们从分子设计角度出发,研发了一系列新型、可注射性的、可生物降解的、原位形成的水凝胶。具体包括：热... 详细信息

＜正＞水凝胶因良好的生物相容性和渗透性等在生物医学领域如药物／蛋白质的缓释、生物组织工程等展现了广阔的应用前景。近年来,我们从分子设计角度出发,研发了一系列新型、可注射性的、可生物降解的、原位形成的水凝胶。具体包括：热敏性的水凝胶、八臂聚乙二

关键词：水凝胶可注射性的可生物降解原位形成蛋白质释放

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