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Murine lung eosinophil activation and chemokine production in allergic airway inflammation

Cellular & Molecular Immunology 2010年第5期7卷 361-374页

作者： C Edward Rose jr joanne A Lannigan Paul Kim james j lee Shu Man Fu Sun-sang j Sung Division of Pulmonary and Critical Care Department of MedicineUniversity of Virginia School of MedicineCharlottesvilleVAUSA Department of Microbiology University of Virginia School of MedicineCharlottesvilleVAUSA Center for Immunity Inflammationand Regenerative Medicine and Division of NephrologyDepartment of MedicineUniversity of Virginia School of MedicineCharlottesvilleVAUSA Division of Pulmonary Medicine Department of Biochemistry and Molecular BiologyMayo Clinic ArizonaScottsdaleAZUSA

Eosinophils play important roles in asthma and lung *** models are widely used for assessing the functional significance and mechanistic basis for eosinophil involvements in these ***,little is known about tissue eosinophils in *** addition,little data on eosinophil chemokine production during allergic airway inflammation are *** this study,the properties and functions of homeostatic and activated eosinophils were *** from normal tissues expressed costimulation and adhesion molecules B7-1,B7-2 and ICAM-1 for Ag presentation but little major histocompatibility complex(MHC)class II,and were found to be poor stimulators of T-cell ***,these eosinophils expressed high levels of chemokine mRNA including C10,macrophage inflammatory protein(MIP)-1a,MIP-1c,MIP-2,eotaxin and monocyte chemoattractant protein-5(MCP-5),and produced chemokine *** intracellular chemokines decreased rapidly with concomitant surface marker downregulation upon in vitro culturing consistent with piecemeal *** eosinophils from mice with induced allergic airway inflammation exhibited increased chemokines mRNA expression and chemokines protein production and upregulated MHC class II and CD11c *** were also found to be the predominant producers of the CCR1 ligands CCL6/C10 and CCL9/MIP-1c in inflamed *** production of C10 and MIP-1c correlated with the marked influx of CD11bhigh lung dendritic cells during allergic airway inflammation and the high expression of CCR1 on these dendritic cells(DCs).The study provided baseline information on tissue eosinophils,documented the upregulation of activation markers and chemokine production in activated eosinophils,and indicated that eosinophils were a key chemokine-producing cell type in allergic lung inflammation.

关键词： allergy chemokines eosinophils lung mouse

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CD69 expression on airway eosinophils and airway inflammation in a murine model of asthma

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Chinese Medical journal 2006年第23期119卷 1983-1990页

作者： WANG Hui-ying SHEN Hua-hao james j lee Nancy A lee Department of Respiratory Medicine Second Affiliated Hospital ofZhejiang University Medical College Institute of RespiratoryDiseases of Zhejiang University Hangzhou 310009 China Department of Biochemistry and Molecular Biology Mayo ClinicScottsdale Scottsdale Arizona 85259 USA

Background Asthma is a chronic airway disease with inflammation characterized by physiological changes （airway hyper-responsiveness, AHR） and pathological changes （inflammatory cells infiltration and mucus production）. Eosinophils play a key role in the allergic inflammation. But the causative relationship between eosinophils and airway inflammation is hard to prove. One of the reasons is lack of activation marker of murine eosinophils. We investigated the expression of CD69 on murine eosinophils in vitro, the relationship between the expression of CD69 on eosinophils from peripheral blood and bronchoalveolar lavage fluid and on airway inflammation in asthmatic mice. Methods Eosinophils from peripheral blood of IL-5 transgenic mice （Nj.1638） were purified. Mice were divided into five groups： wild type mice sensitized and challenged with saline （WS group）, wild type mice sensitized and challenged with ovalbumin （WO group）, IL-5^-/- mice sensitized and challenged with saline and transferred with purified eosinophils （ISE group）, IL-5^-/- mice sensitized and challenged with OVA and transferred with purified eosinophils （IOE group）, IL-5^-/- mice sensitized and challenged with OVA and transferred with purified eosinophils, pretreated with anti CD4 monoclonal antibody （IOE＋antiCD4mAb group）. IL-5^-/- mice were sensitized with OVA at day 0 and day 14, then challenged with OVA aerosol. On days 24, 25, 26 and 27 purified eosinophils were transferred intratracheally to IL-5^-/- mice. On day 28, blood and BALF were collected and CD69 expression on eosinophils measured by flowcytometry. Results Purified eosinophils did not express CD69. But eosinophils cultured with PMA＋MA, IFN- T, IL-5 or GM-CSF expressed CD69 strongly. Eosinophils from blood of WO, WS group did not express CD69 at all. The numbers of eosinophils in BALF of WO group, IOE group, ISE group and IOE＋antiCD4mAb group were significantly higher than in mice of WS group which did not have eosinophils at all. CD69

关键词： asthma eosinophils CD69 airway inflammation murine model

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CD69的表达在小鼠嗜酸细胞的活化与凋亡中的作用

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中国病理生理杂志 2009年第1期25卷 1-6页

作者：汪慧英 james j lee Nancy A lee 浙江大学医学院附属二院呼吸内科浙江大学呼吸病研究所浙江杭州310009 Department of Biochemistry and Molecular Biology Mayo Clinic - Scottsdale Scottsdale Arizona 85259 USA

目的:观察在体内、体外不同条件下小鼠嗜酸细胞(EOS)表面CD69的表达与细胞存活率,探讨CD69的表达在小鼠EOS的活化、凋亡中的作用。方法:提纯IL-5高分泌转基因小鼠外周血中的EOS,测定其表面CD69的表达,体外以PMA+MA刺激EOS,在1 h、12 h、... 详细信息

目的:观察在体内、体外不同条件下小鼠嗜酸细胞(EOS)表面CD69的表达与细胞存活率,探讨CD69的表达在小鼠EOS的活化、凋亡中的作用。方法:提纯IL-5高分泌转基因小鼠外周血中的EOS,测定其表面CD69的表达,体外以PMA+MA刺激EOS,在1 h、12 h、18 h、24 h测定细胞表面CD69的表达与细胞存活率;分别以1μg/L的IL-4、IL-5、IL-12、IL-13、IFN-γ、GM-CSF培养EOS18 h后测定细胞的存活率与表面CD69的表达。制备小鼠哮喘模型,观察CD69在小鼠BALF、外周血EOS中的表达。结果:新鲜提纯的小鼠外周血EOS不表达CD69,PMA+MA刺激的EOS在1 h后即有CD69的表达,12 h表达至高峰,至少持续24 h以上;但细胞的存活率快速下降。不同细胞因子对EOS的培养均可诱导CD69的表达,其中IL-13、IFN-γ、GM-CSF对此影响明显,且GM-CSF可显著抑制EOS的凋亡;哮喘小鼠外周血EOS无CD69的表达,而BALF中EOS可有CD69的表达。结论:静止小鼠EOS表面不表达CD69,但在体内、体外不同条件下激活的EOS表面均有CD69的表达;同时,体外实验显示CD69的表达与细胞凋亡密切相关。结果提示CD69既可作为EOS激活的表面标记物,同时又可诱导EOS的凋亡,这为哮喘治疗提供新的思路。

关键词： CD69 嗜酸细胞细胞凋亡

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Seasonal Distributions of Anhydrosugars Derived from Rice Straw Burning

Seasonal Distributions of Anhydrosugars Derived from Rice St...

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第十届全国气溶胶会议暨第六届海峡两岸气溶胶技术研讨会

作者： james j.lee Department of Safety Health and Environmental Engineering National Yunlin University of ScienceDouliouTaiwan

Aerosol particles released from biomass and bio-fuel burning have large contributions to the global aerosol *** primary agricultural commodity in Taiwan is rice,similar to most countries in *** a long-term study collected between December 2006 and February 2008 in Yunlin County on the subtropical island of Taiwan. Ambient aerosol was examined for seasonal patterns（summer harvest *** harvest）in terms of size-resolved chemical *** concentrations,size distributions and diagnostic ratios of three anhydrosugars（levoglucosan,mannosan,and galactosan）,used as molecular tracers for biomass burning,are reported *** total of the three anhydrosugars（ΣAnhydrosugars）in PM2.5,PM10,and TSP during the winter harvest season was on average 478（±270）,494（±275）,and 618（±385）ng m -3 ,respectively,while the respective summer harvest season values were 449（±170）,888（±271）,and 1125（±391）ng m -3 .Levoglucosan concentrations showed a strong seasonal dependence in the ambient levels as well as size *** high PM2.5-10 fraction of levoglucosan during the summer harvest season is likely related to the wet ambient conditions,characteristic for summer time in southern *** addition to particle coagulation,uptake of water by ambient aerosol particles will result in increased particle size as ***,Levoglucosan/mannosan ratios in PM2.5 derived from rice straw burning processes revealed a clear signature for smoke aerosol from straw burning.

关键词： Biomass burning Rice straw Levoglucosan Particle size distributions

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Characteristics of Sized-resolved Anhydrosugars and Pollutants Derived from Rice Straw Burning in Controlled Chamber

Characteristics of Sized-resolved Anhydrosugars and Pollutan...

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第七届亚洲气溶胶会议

作者： Chih-jen Chuang james j. lee Engineering Science and Technology National Yunlin University of Science and Technology Environmental Health and Safety Engineering National Yunlin University of Science and Technology

This study discusses the particle-sized emission characteristics in three combustion conditions (smoldering, flaming, and mixing conditions). The purpose of this paper is to investigate of characterize particle-sized profiles for the compositions of particulate mass, carbon species (OC and EC), three anhydrosugars (levoglucosan, mannosan, and galactosan) and water-soluble inorganic derived from rice straw burning in a controlled chamber. The results have shown that the smoldering smoke releases the highest particulate mass concentration in three combustion conditions. The particle-size emission of rice straw burning was concentrated in PM2.5 particles (667% of TSP).Levoglucosan constituent accounts 90% in three anhydrosugars from rice straw burning in the experiment. The ratio range of levoglucosan to mannosan (Levo/Mann) from rice straw burning was approximately 25-40 resulting from cracking cellulose composition in several burning *** carbonaceous composition from rice straw burning was mainly consisted in organic carbon (OC>90%), indicated that rice straw burning belongs to incomplete combustion. Water-soluble ions of particles from rice straw burning significantly abound in spices of Cl-, F-, SO42-, K+, and NH4+. The higher CO/CO2 value (>11%) was belong to smoldering condition of rice straw burning. Therefore, the study indicated that levoglucosan might be assigned as a unique molecular tracer and very helpful to identify rice straw burning emission characteristics in the future.

关键词： Biomass burning Levoglucosan Particle-size distribution Ions Carbon dioxide

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XAD-4微孔介质半挥发性PAHs吸附机制特征的研究

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过程工程学报 2006年第Z2期6卷 150-154页

作者：李经民庄智仁云林科技大学环境与安全卫生工程系

以"吸附相"机制探讨温度效应(30,50,70℃)影响XAD-4七种半挥发性PAHs(AcPy,AcP,Flu,PA,Ant,Fl,Pyr)吸附滞留特征.实验结果显示,随温度30℃升至70℃,XAD-4微孔介质七种PAHs单位吸附滞留体积(Vg)由31.8～58.5m3降至19.5～24.6m3;高温环境(70℃)PAHs微孔介质吸附可能已由低温环境(30℃)孔径吸附转变为表面吸附.此外,讨论了七种气相PAHs化合物单位吸附滞留体积(Vg)与其过冷液蒸汽压(PL)相关性,结果显示两者呈负相关,PAHs已经不是气态型吸附式而是以纳米液态气胶进行微孔吸附行为.本研究结果显现微孔介质比表面积不是PAHs吸附效能唯一关键因子,温度效应与介质孔径效应都可能影响PAHs微孔吸附效能与介质吸附型式(表面吸附vs.孔径吸附).

关键词：半挥发性有机化合物温度效应孔径吸附纳米气胶

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Generation of brain vascular heterogeneity:recent advances from the perspective of angiogenesis

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Neural Regeneration Research 2025年第7期20卷 2013-2014页

作者： Nathanael j.lee Ryota L.Matsuoka Department of Neurosciences Lerner Research InstituteCleveland ClinicClevelandOHUSA Department of Molecular Medicine Cleveland Clinic Lerner College of MedicineCase Western Reserve UniversityClevelandOHUSA

Heterogeneous proper t i es of vascular endothelial cells in the brain:The brain displays large energy dynamics and consumption,and this high level of metabolic demands is fulfilled by a continuous supply of glucose and oxygen through its vascular *** vasculature consists of highly divergent blood vessel branches,giving rise to a dense network of capillaries that supply blood to all cells across the *** elaborated vascular network is thought to develop via angiogenesis,a process in which new blood vessels grow from pre-existing *** capillaries exhibit organotypic features distinct from other tissues and are formed primarily by two major endothelial cell(EC)types:those that form the semi-permeable blood-brain barrier(BBB)and those that develop highly permeable pores known as fenestrae(Matsuoka et al.,2022).The structural and functional differences between BBB and fenestrated vascular ECs represent a fundamental feature of brain vasculature and form the foundation for both brain function and homeostasis.

关键词： angiogenesis homeostasis network

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Neuroprotection by resveratrol-glucuronide and quercetin-glucuronide via binding to polyphenol-and glycosaminoglycan-binding sites in the laminin receptor

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Neural Regeneration Research 2025年第3期20卷 819-820页

作者： Rayudu Gopalakrishna jennifer Aguilar Emily lee William j.Mack Department of Integrative Anatomical Sciences Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCAUSA Department of Neurological Surgery Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCAUSA

The dietary polyphenolic compounds resveratrol and quercetin prevent neurodegenerative diseases in experimental models;however, they reach the brain only in nanomolar concentrations in the glucuronidated and sulfated ... 详细信息

关键词： resveratrol molar dated

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滤材截留微粒中PAHs吹出效应之研究

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过程工程学报 2004年第Z1期4卷 859-863页

作者：李经民井长瑞陈玉恭郑惠裕云林科技大学环境与安全研究所云林云林科技大学环境与安全研究所云林云林科技大学环境与安全研究所云林云林科技大学环境与安全研究所云林

滤材截留粒状物中因采集气流常造成固相PAHs有吹出现象,低估微粒中PAHs浓度值,影响其对人体健康风险评估的正确性.实验藉由自行设计滤纸吹出实验(Blow-off)装置,以7种PAHs同位素建立悬浮微粒中PAHs化合物吹出比值(Blow-off,θ),并解析... 详细信息

滤材截留粒状物中因采集气流常造成固相PAHs有吹出现象,低估微粒中PAHs浓度值,影响其对人体健康风险评估的正确性.实验藉由自行设计滤纸吹出实验(Blow-off)装置,以7种PAHs同位素建立悬浮微粒中PAHs化合物吹出比值(Blow-off,θ),并解析不同流速(20～60 cm/s)及温度(25～45℃)条件对固相PAHs吹出比值(θ)影响.实验结果显示少环PAHs化合物(如NaP,AcP)吹出比值明显较多环PAHs物质(如F1,Pyr)为大,随表面流速上升其吹出现象有增加趋势.少环PAHs化合物较易吹出但受温度影响较少(随温度上升θ增加仅约4%～17%);多环PAHs化合物吹出比值较低但随温度升高(25→45℃)吹出比值明显倍增(15%～34%增至32%～58%).

关键词：半挥发性有机化合物吹出现象采样误差风险评估室内空气清净技术

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Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

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Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal j.Goldschmidt-Clermont Aisha Khan George jimsheleishvili Patricia Graham Adriana Brooks Risset Silvera Alexander j.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi james D.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

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