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Cross-sectional study to determine viral hepatitis knowledge in different urban populations in Brazil

World Journal of Hepatology 2018年第11期10卷 867-876页

作者： Helena Medina Cruz jakeline ribeiro barbosa Jeová Keny Baima Colares Antonio Henrique Almeida de Moraes Neto Maria de Fátima Leal Alencar Francisco Inácio Bastos Jurema Corrêa da Mota Filipe Aníbal Carvalho-Costa Claudia Alexandra Pontes Ivantes Lia Laura Lewis-Ximenez Livia Melo Villar Laboratory of Viral HepatitisOswaldo Cruz Institute Fiocruz Rio de Janeiro 21040360 Brazi Postgraduate Program in Pathology Federal University of Ceará Fortaleza Ceará 60020181 Brazil Postgraduate Program in Medical Sciences University of Fortaleza Ceará 60430160 Brazil Laboratory of Innovations in Therapies Teaching and Bioproducts Oswaldo Cruz Institute Fiocruz Rio de Janeiro 21040360 Brazil Institute of Communication and Scientifc Information and Technology for Health Oswaldo Cruz Foundation Rio de Janeiro 21040900 Brazil Laboratory of Epidemiology and Molecular Systematics Oswaldo Cruz Institute Fiocruz Rio de Janeiro 21040900 Brazil Orientation and Counselling Centre Curitiba Paraná 80810070 Brazil

AIM To evaluate viral hepatitis knowledge among individuals from different resource areas and health conditions to identify possible *** A cross-sectional, descriptive study was carried out among 447 individuals from five distinct populations in Brazil: Southeast Viral Hepatitis Ambulatory(n = 100), South(n = 89) and Northeast(n = 114) Health Center, Southeast(n = 77) and Northeast(n = 67) low resource areas. All individuals answered a questionnaire assessing sociodemographic characteristics and viral hepatitis awareness. The perception was scored based on the average number of correct answers of all participants and categorized as "low"(0-28 correct answers) or "desirable"(29-46 correct answers). Associations between sociodemographic characteristics and perception were also *** A low level of knowledge was observed in individuals from Northeast Health Center, Northeast and Southeast low resource areas while desirable knowledge was observed in individuals from Viral Hepatitis Ambulatory and South Health Center. According to sociodemographic characteristics, desirable scores were more common among those with secondary education(47.1%), those who declared themselves as white(46.3%), and those who lived in houses with three individuals(25.5%). Multivariate analysis showed an association between viral hepatitis perception and type of population. CONCLUSION The results demonstrated high level of knowledge among study participants from health clinics from the Southeast region of Brazil and the importance of education programs in increasing the level of knowledge in low resource areas.

关键词： Viral hepatitis Knowledge Perception Urban population Brazil

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Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations

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World Journal of Hepatology 2021年第4期13卷 504-514页

作者： Geane Lopes Flores jakeline ribeiro barbosa Helena Medina Cruz Juliana Custódio Miguel Denise Vigo Potsch JoséHenrique Pilotto Danielle Malta Lima JeováKeny Baima Colares Carlos Eduardo Brandão-Mello Marcia Maria Amendola Pires Jurema Corrêa da Mota Francisco I Bastos Lia Laura Lewis-Ximenez Livia Melo Villar Viral Hepatitis Laboratory FIOCRUZRio de Janeiro 21040360Brazil Postgraduate Program in Pathology Federal University of CearáFortaleza 60020181CearaBrazil Clementino Fraga Filho Hospital Federal University of Rio de JaneiroRio de Janeiro 21040360Brazil Nova Iguaçu General Hospital Nova Iguaçu 26041061Brazil Molecular Immunology Laboratory Oswaldo Cruz InstituteRio de Janeiro 21040360Brazil Postgraduate Program in Medical Sciences University of FortalezaFortaleza 60811905CearáBrazil Escola de Medicina e Cirurgia Gaffrée&Guinle University HospitalFederal University of Rio de Janeiro StateRio de Janeiro 20270901Brazil Institute of Communication and Information on Science and Technology in Health FIOCRUZRio de Janeiro 21040360Brazil

BACKGROUND To achieve the elimination of hepatitis B and C,there is an urgent need to develop alternative strategies to increase the access of diagnosis,particularly among key populations such as people living with human immunodeficiency virus(HIV),individuals with coagulopathies and chronic kidney disease(CKD)*** To evaluate the use of dried blood spot(DBS)in the detection of hepatitis B virus(HBV)and hepatitis C virus(HCV)*** A total of 430 individuals comprised of people living with HIV,coagulopathies and CKD provided paired serum and DBS ***,anti-HBc and anti-HCV were tested in those samples using a commercial *** and selected behavioral variables were evaluated to assess possible association with HBV and HCV *** Using DBS,HBsAg prevalence varied from 3.9%to 22.1%,anti-HBc rates varied from 25.5%to 45.6%and anti-HCV positivity ranged from 15.9%to 41.2%in key *** of HBV and HCV tests using DBS varied from 88.9%to 100%.The HBsAg assay demonstrated the best performance in CKD and coagulopathy individuals and the anti-HCV test had a sensitivity and specificity of 100%in people living with *** of HBV and HCV detection in DBS varied from 90.2%to 100%.In the CKD group,HBsAg positivity was associated with infrequent use of condoms,and anti-HBc positivity was associated with sharing nail cutters/razors/***-HCV reactivity was positively associated with a history of transplantation and length of time using hemodialysis in both *** people living with HIV,only the male gender was associated with anti-HBc positivity in serum and *** DBS with electrochemiluminescence are useful tools for the diagnosis and prevalence studies of hepatitis B and C among key populations and may increase the opportunity to foster prevention and treatment.

关键词： Dried blood spot Electrochemiluminescence Hepatitis B Hepatitis C Key populations Diagnosis

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Update on hepatitis B and C virus diagnosis

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World Journal of Virology 2015年第4期4卷 323-342页

作者： Livia Melo Villar Helena Medina Cruz jakeline ribeiro barbosa Cristianne Sousa Bezerra Moyra Machado Portilho Letícia de Paula Scalioni Viral Hepatitis Laboratory Oswaldo Cruz Institute Oswaldo Cruz Foundation

Viral hepatitis B and C virus(HBV and HCV) are responsible for the most of chronic liver disease worldwide and are transmitted by parenteral route, sexual and vertical transmission. One important measure to reduce the burden of these infections is the diagnosis of acute and chronic cases of HBV and HCV. In order to provide an effective diagnosis and monitoring of antiviral treatment, it is important to choose sensitive, rapid, inexpensive, and robust analytical methods. Primary diagnosis of HBV and HCV infection is made by using serological tests for detecting antigens and antibodies against these viruses. In order to confirm primary diagnosis, to quantify viral load, to determine genotypes and resistance mutants for antiviral treatment, qualitative and quantitative molecular tests are used. In this manuscript, we review the current serological and molecular methods for the diagnosis of hepatitis B and C.

关键词： Diagnostic methods Genotypes Hepatitis B virus Molecular diagnostic techniques Serological tests Hepatitis C virus

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Soil Water Availability on Growth and Development of Safflower Plants

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American Journal of Plant Sciences 2015年第13期6卷 2066-2073页

作者： Edna Maria Bonfim-Silva Ellen Cristina Alves de Anicésio jakeline Rosa de Oliveira Helon Hébano de Freitas Sousa Tonny José Araújo da Silva Department of Agricultural and Environmental Engineering Institute of Agricultural Sciences and Technology Federal University of Mato Grosso Rondonópolis Brazil

Safflower (Carthamus tinctorius L.) is a promising culture to be widespread in Brazil. However, the lack of basic knowledge about cultivation techniques, such as water demand by the culture, is still obstacle to the expansion of safflower in that country. The objective was, then, to evaluate the effect of the soil water availability on growth and development of safflower in the Cerrado soil of Mato Grosso, Brazil. The experiment was conducted in a greenhouse, in a completely randomized design with five water availabilities (25%, 50%, 75%, 100% and 125% of the maximum water holding capacity in the soil) and four replications. Maintenance soil moisture was performed by gravimetric method with daily weighing of experimental units. The variables analyzed were: plant height, stem diameter, number of leaves, number of heads, heads diameter, dry mass of shoots, heads, and roots. The results were submitted to analysis of variance and regression test at 5% probability by SISVAR program. All variables set to the quadratic regression model, showing the best results in the water availability between 64% and 76%. Safflower is shown to be more sensitive to water stress with increased tolerance to water deficit in the soil than to flooding.

关键词： Carthamus tinctorius L. Field Capacity Water Stress IMA 0213

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Soil Water Availabilities in the Content and Accumulation of Nitrogen and Chlorophyll Index in the Safflower

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American Journal of Plant Sciences 2015年第14期6卷 2280-2286页

The safflower (Carthamus tinctorius L.) is adapted to grow in adverse conditions, is tolerant to salinity and water deficit, however, its productivity increases when grown in areas with greater availability of water and in fertilized conditions, showing that the knowledge of crop management can bring income gains for producers. It is known that extreme conditions of soil moisture damage the crop development. Given this context, the objective was to evaluate the influence of water availability on the nutritional characteristics of the safflower. The experiment was conducted in a greenhouse in a completely randomized design with five water availabilities (25%, 50%, 75%, 100% and 125% of the maximum water holding capacity of the soil) in four replications. Maintaining soil moisture was performed by gravimetric method with daily weighing of experimental units. The variables analyzed were SPAD reading (chlorophyll content), concentration and accumulation of nitrogen in the shoot and in the sections. The results were submitted to analysis of variance and regression test at 5% probability by Sisvar program. There was adjusting of variables to linear and quadratic regression models. Water availabilities between 65% and 75% have greater potential to promote better nutrition for safflower plants. Both deficit and excess of water in the soil are detrimental to the absorption of nitrogen and reduce the chlorophyll content. Safflower is less tolerant to excess than to deficit of water.

关键词： Carthamus tinctorius L. Nutrition SPAD Irrigation

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