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Virologica Sinica 2013年第1期28卷 36-42页

作者： Julian Ruiz-Sáenz Jairo jaime Victor Vera Microbiology and Epidemiology Group Faculty of Veterinary Medicine and Animal Science National University of Colombia Grupo de Investigación CENTAURO Agrarian Sciences Faculty Universidad de Antioquia A.A. 1226

Bovine Herpesvirus-1 （BoHV-1） is a DNA virus belonging to the family Herpesviridae, subfamily Alfaherpesvirinae; it is a worldwide pathogen, causing serious economic losses in livestock. In Colombia there have been multiple isolates of BoHV-1 that have been subjected to molecular characterization, classifying most of the country isolates as BoHV-I.1. In the present study we developed and evaluated an ethyleneimine binary inactivated isolate from the native BoHV-1 strain （C6rdoba-2） in a rabbit model of vaccination and infection. The vaccine was evaluated in two phases, one of immunogenicity with vaccination and a booster after 21 days, and an evaluation phase of protection against challenge with a highly virulent reference strain. The results demonstrate optimum serum-conversion, with protective neutralizing antibody titers 28 days post vaccination and optimal protection against challenge with the reference strain with decreased clinical signs of infection, protection against the onset of fever and decrease of virus excretion post challenge. In conclusion, our results show the enormous potential that an immunogenic inactivated vaccine has produced from the native BoHV-I.1 strain, which produces a high antigen mass to the vaccine to induce optimal immunity and protection, and it is a strong candidate for evaluation and possible future use in different cattle populations.

关键词： Antigenic Mass Adjuvant Binary Ethyleneimine Bovine Herpesvirus-1 Vaccine

Unmanned Aerial Vehicle Multi-Access Edge Computing as Security Enabler for Next-Gen 5G Security Frameworks

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Intelligent Automation & Soft Computing 2023年第8期37卷 2307-2333页

作者： jaime Ortiz Córdoba Alejandro Molina Zarca Antonio Skármeta Department of Information and Communications Engineering University of MurciaMurcia30003Spain

5G/Beyond 5G(B5G)networks provide connectivity to many heterogeneous devices,raising significant security and operational issues and making traditional infrastructure management increasingly *** this regard,new frameworks such as Anastacia-H2020 or INSPIRE-5GPlus automate the management of next-generation infrastructures,especially regarding policy-based security,abstraction,flexibility,and *** paper presents the design,workflow,and implementation of a security solution based on Unmanned Aerial Vehicles(UAVs),able to extend 5G/B5G security framework capabilities with UAV features like dynamic service provisioning in specific geographic *** proposed solution allows enforcing UAV security policies in proactive and reactive ways to automate UAV dynamic deployments and provisioning security Virtual Network Functions(VNFs)in the onboard Multi-access Edge Computing(MEC)node.A UAV has been ensembled from scratch to validate the proposal,and a raspberry-pi has been onboarded as compute *** implementation provides a VNF for dynamic UAV management,capable of dynamically loading waypoints into the flight controller to address reactive autonomous flights,and anMLbased VNF capable of detecting image *** to the security policies,the onboard VNFs can be dynamically configured to generate alerts to the framework and apply local reactions depending on the detection *** our experiments,we measured the time it takes for the solution to be ready after receiving a security policy for detecting patterns in a specific geographical *** time it takes for the solution to react automatically was also *** results show that the proactive flow configuration considering ten waypoints can be enforced in less than 3 s,and a local reactive flow can be enforced in around 1 *** consider that the results are promising and aligned with other security enabler solutions as part of existing 5G/6G security frameworks.

关键词： UAV 5G AI NFV security

Molecular and in vitro Characterization of Field Isolates of Bovine Herpesvirus-1

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Virologica Sinica 2012年第1期27卷 26-37页

作者： Julian Ruiz-Saenz Jairo jaime Gloria Ramirez Victor Vera Faculty of Veterinary Medicine and Animal Science National University of Colombia

Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.

关键词： Bovine herpesvirus 1 Genital infection Respiratory infection Latent infection

Variations in porcine colostrum oligosaccharide composition between breeds and in association with sow maternal performance

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Journal of Animal Science and Biotechnology 2020年第3期11卷 830-840页

作者： Paolo Trevisi Diana Luise Savanna Won jaime Salcedo Micol Bertocchi Daniela Barile Paolo Bosi Department of Agricultural and Food Sciences University of BolognaViale G.Fanin 4640127 BolognaItaly Department of Food Science and Technology University of California-DavisOne Shields AvenueDavisCA 95616USA

Background:Oligosaccharides(OS)are indigestible carbohydrates naturally found in *** composition of porcine colostrum OS may influence the growth and the health of the neonate and consuming optimal concentrations of OS may reduce piglet susceptibility to *** this manner,targeted supplementation of animal feed with OS is being explored as a health management tool in the livestock *** variation in OS composition between different breeds of pig and its association with the litter performance is currently *** aim of this study was to characterize the colostrum OS composition from sows of different breed and parity and correlate this data with sow maternal ***:Eighty-three colostrum samples from parities 1 to 8 were gathered from 3 different breeds of sow:44 Large White sows,27 Landrace sows and 12 Duroc *** were taken between the birth of the first and the last piglet from sows that were not pharmacologically induced to *** were purified from the samples and analysed by MALDI-ToF mass spectrometry(21 OS compositions detected).The farrowing season and the maternal data were recorded for each sow,including the number of live piglets and the litter body weight at birth,at day(d)3 and at ***:Five OS compositions,including isomers of the bifidogenic Sialyllactose,Lacto-N-Tetraose and Lacto-NHexaose series,were detected in all the *** other OS were identified in at least 50%of samples,and their abundances were affected by breed(P<0.05;6 of 12),marginally affected by season(P<0.10;3 of 12)and never by parity *** abundances of each OS component were standardized by Z-score scaling(μ=0 and SD=1),transformed by principal component analysis,and four similarity clusters were *** membership was associated with litter weight gain within 3 days(P=0.063)and at weaning(P<0.05),but not with piglet mortality within 3 ***:OS composition of colostrum may partially explain the vari

关键词： MALDI-ToF analysis Milk Nutrient composition Pig breeds Piglet survival

Factors affecting somatic embryogenesis in conifers

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Journal of Forestry Research 2012年第4期23卷 503-515页

作者： jaime A.Teixeira da Silva Ravindra B.Malabadi Faculty of Agriculture and Graduate School of Agriculture Kagawa UniversityMiki-choIkenobe 2393Kagawa-ken761-0795Japan Division of Plant Biotechnology Department of BotanyKarnatak UniversityPavate NagarDharwad-580003Karnataka StateIndia

This review seeks to examine the extreme response of isolated somatic plant cells of apical meristematic tissues of mature conifer trees towards specific stress conditions in vitro resulting in somatic embryo- genesis. Signal molecules regulating embryo development have been described in angiosperms, but very little is known about somatic rejuve- nation in conifers. Recent studies on cloning of mature conifers provide new perspectives on signal molecules on cellular dedifferentiation into the embryogenic pathway. Our recent studies show that signal molecules such as butenolide, calcium ions, salicylic acid, antioxidants, amino acids triacontanol and 24-epibrassinolide all play an important role in the con- version of somatic cells into an embryogenic pathway in many recalcitrant pines. This constitutes a major breakthrough in forest biotechnology with many practical applications in clonal forestry.

关键词： cloning commercial forestry in vitro micropropagation thin cell layers

The ability of sperm selection techniques to remove single- or double-strand DNA damage

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Asian Journal of Andrology 2011年第5期13卷 764-768页

作者： Maria Enciso Miriam Iglesias Isabel Galan Jonas Sarasa Antonio Gosalvez jaime Gosalvez Unidad de Genetica Departamento de Biologia Universidad Aut6noma de Madrid Madrid 28049 Spain Unidad de Reproducci6n Asistida Hospital Quir6n Madrid Madrid 28223 Spain

A wide variety of techniques for the preparation of sperm are currently available, of which the most commonly employed are densitygradient centrifugation （DGC） and swim-up （SUP）. To date, these methods appear to be effective in selecting functional sperm for assisted reproduction techniques （ART）, but they may have negative effects on sperm DNA. In this study, the ability of these semen processing techniques to eliminate spermatozoa containing single- and double-strand DNA damage was assessed by the two-tailed comet assay and the sperm chromatin dispersion test in 157 semen samples from patients seeking assisted reproduction treatment. Our results indicated that SUP and DGC are equally efficient in eliminating spermatozoa containing double-strand DNA damage and sperm with highly damaged （degraded） DNA, as characterized by the presence of both single- and double-strand DNA breaks. However, DGC is more efficient than SUP in selecting spermatozoa that are free from single-strand DNA damage. Future studies should characterise the importance of the various types of DNA damage and examine the sperm processing protocols used in each laboratory to determine their ability to eliminate DNA damage and hence, prevent the potential transmission of genetic mutations via ART.

关键词： density-gradient centrifugation male factor sperm chromatin dispersion sperm DNA fragmentation swim-up

Soil mineral nitrogen dynamics in fallow periods in a rainfed semiarid Mediterranean agricultural system

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Pedosphere 2023年第4期33卷 622-637页

作者： Awais SHAKOOR Àngela D.BOSCH-SERRA Antonio LIDON Damián GINESTAR jaime BOIXADERA University of Lleida Department of Environment and Soil SciencesAvda.Alcalde Rovira Roure 191Lleida E-25198(Spain) Institute of Water and Environmental Engineering Universitat Politècnica de ValènciaCamino de Vera s/n.Valencia E-46022(Spain) Institute for Multidisciplinary Mathematics Universitat Politècnica de ValènciaCamino de Vera s/n.Valencia E-46022(Spain) Department of Agriculture LivestockFisheries and FoodGovernment of CataloniaAvda.Alcalde Rovira Roure 191Lleida E-25198(Spain)

Rainfed agricultural systems in semiarid Mediterranean environments are subject to erratic but often heavy rainfall *** an agronomic practice,fallow periods can be included even within the existing European Union common policy for crop *** study aimed to quantify the effects of previous mineral fertilization on soil mineral nitrogen(N_(min))content and potential nitrate leaching during no-till fallow periods of crop *** Leaching Estimation and Chemistry Model(LEACHM)was used for soil N_(min)prediction after *** fallow periods,N_(min)measured in the soil profile increased by an average of 125 kg N ha^(-1),whereas the model showed an average increase of 95 kg N ha^(-1).The amount of nitrogen(N)leached was estimated to be 11–38 kg N ha^(-1).The N balance simulated using LEACHM might differ from the actual *** calibrated LEACHM underestimated soil N_(min)after a drought period followed by soil water replenishment,probably due to a pulse in soil C and N mineralization caused by the wetting of dry soil,but overestimated soil N_(min)after occasional rainy spells when soil quickly became saturated,probably due to occasional nitrous oxide emissions not being fully accounted for by the model and specific preferential water flow,which might lead to greater nitrate leaching than that simulated by LEACHM using the convection-dispersion *** results show that soil N_(min)measurements after fallow periods cannot be well predicted by *** findings of this study provide a support for fertilization planning for crop rotation systems including a fallow period to avoid overfertilization in the following cropping season and reduce N environmental impacts.

关键词： crop rotation Latin Hypercube-One factor at a Time(LH-OAT)method Leaching Estimation and Chemistry Model(LEACHM) mineral fertilizer nitrogen balance nitrogen environmental impacts nitrogen leaching soil water

Integration of Facial Thermography in EEG-based Classification of ASD

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International Journal of Automation and computing 2020年第6期17卷 837-854页

作者： Dilantha Haputhanthri Gunavaran Brihadiswaran Sahan Gunathilaka Dulani Meedeniya Sampath Jayarathna Mark jaime Christopher Harshaw Department of Computer Science and Engineering University of MoratuwaMoratuwa 10400Sri Lanka Department of Computer Science Old Dominion UniversityNorfolk 23529USA Division of Science Indiana University-Purdue UniversityIndianapolis 47203USA Department of Psychology University of New OrleansNew Orleans 7014&USA

Autism spectrum disorder(ASD)is a neurodevelopmental disorder affecting social,communicative,and repetitive *** phenotypic heterogeneity of ASD makes timely and accurate diagnosis challenging,requiring highly trained clinical *** development of automated approaches to ASD classification,based on integrated psychophysiological measures,may one day help expedite the diagnostic *** paper provides a novel contribution for classifing ASD using both thermographic and EEG *** methodology used in this study extracts a variety of feature sets and evaluates the possibility of using several learning ***,standard deviation,and entropy values of the EEG signals and mean temperature values of regions of interest(ROIs)in facial thermographic images were extracted as *** selection is performed to filter less informative features based on *** classification process utilizes Naive Bayes,random forest,logistic regression,and multi-layer perceptron *** integration of EEG and thermographic features have achieved an accuracy of 94%with both logistic regression and multi-layer perceptron *** results have shown that the classification accuracies of most of the learning models have increased after integrating facial thermographic data with EEG.

关键词： Autism spectrum disorder facial thermography EEG signal processing machine learning decision support system ASDGenus.

A fuzzy cognitive map-based algorithm for predicting water consumption in Spanish healthcare centres

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Building Simulation 2023年第11期16卷 2193-2205页

作者： Gonzalo Sánchez-Barroso jaime González-Domínguez Joao Paulo Almeida-Fernandes Justo García-Sanz-Calcedo Engineering Projects Area University of ExtremaduraAv.Elvas06006BadajozSpain Department of Paisagem Ambiente e OrdenamentoUniversity of EvoraR.Romão Ramalho7000-650ÉvoraPortugal

The management of water consumption in healthcare centres can have positive impacts on both the environmental performance and profitability of health *** tools assist in the decision-making process of managing the operation and maintenance of healthcare *** research aimed to integrate the empirical knowledge of experts in Healthcare Engineering and the historical data from 66 healthcare centres in a Fuzzy Cognitive *** outputs of the predictive model included water consumption,water cost,and CO_(2) emissions in healthcare facilities,along with eleven variables to discover the causes and consequences of water consumption in healthcare centres.A healthcare centre with about 12350 users,located in a city that experiences an average of 1100 heating degree days,whose facilities be moderately energy-efficient contributing over 50%with renewable energies is expected to consume 8.4 dam^(3) of water with 32.1 k€of cost,and contribute realising 30.8 ton CO_(2)eq *** use of Fuzzy Cognitive Maps for prediction can provide a high level of effectiveness in identifying the factors that contribute to water consumption and in designing key performance indicators to manage the environmental performance of healthcare *** tool is extremely effective in enhancing the performance of the management division of health systems.

关键词： water consumption Fuzzy Cognitive Maps healthcare centres Healthcare Engineering water management

Red sandalwood(Pterocarpus santalinus L. f.): biology,importance, propagation and micropropagation

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Journal of Forestry Research 2019年第3期30卷 745-754页

作者： jaime A.Teixeira da Silva Mafatlal M.Kher Deepak Soner M.Nataraj P. O. Box 7 Miki-cho post officeIkenobe 3011-2 Kagawa-ken 761-0799Japan P.G.Department of Biosciences Sardar Patel UniversitySardar Patel MaidanVadtal Rd.P.O.Box 39 Vallabh VidyanagarGujarat 388120India Resonance Plot No-21/5Near Regional Science CentreAcharya ViharBhubaneswarOdisha 751013India

Pterocarpus santalinus L. f.(Fabaceae;red sanders) is prized for its wood whose colour and fragrance is due to the presence of santalins that have pharmaceutical and industrial uses. Red sanders is listed as an endangered plant species on the IUCN red data list as a result of the exploitation of its wood and essential oil. This review emphasizes the pollination biology, seed germination, vegetative propagation and micropropagation of P. santalinus. Excessive use of P. santalinus has also caused the emergence of various adulterants, so accurate identification is essential.

关键词： Conservation Fabaceae IUCN red data list Medicinal plant Micropropagation Red sanders