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α1-COP modulates plasmodesmata function through sphingolipid enzyme regulation

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Journal of Integrative Plant Biology 2024年第8期66卷 1639-1657页

作者： Arya Bagus Boedi Iswanto Minh Huy Vu Jong Cheol Shon Ritesh Kumar Shuwei Wu Hobin Kang Da-Ran Kim Geon Hui Son Woe Yoen Kim youn-Sig Kwak Kwang Hyeon Liu Sang Hee Kim jae-Yean Kim Division of Applied Life Science(BK21 FOUR Program) Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 52828Korea College of Pharmacy and Research Institute of Pharmaceutical Sciences Kyungpook National UniversityDaegu 702-701Korea Departement of Plant Medicine Gyeongsang National UniversityJinju 52828Korea Division of Life Science Gyeongsang National UniversityJinju 52828Korea

Callose,aβ-1,3-glucan plant cell wall polymer,regulates symplasmic channel size at plasmodesmata(PD)and plays a crucial role in a variety of plant ***,elucidating the molecular mechanism of PD callose homeostasis is *** screened and identified an Arabidopsis mutant plant with excessive callose deposition at PD and found that the mutated gene wasα1-COP,a member of the coat protein I(COPI)coatomer *** report that loss of function ofα1-COP elevates the callose accumulation at PD by affecting subcellular protein localization of callose degradation enzyme Pd *** process is linked to the functions of ERH1,an inositol phosphoryl ceramide synthase,and glucosylceramide synthase through physical interactions with theα1-COP ***,the loss of function ofα1-COP alters the subcellular localization of ERH1 and GCS proteins,resulting in a reduction of Glc Cers and Glc HCers molecules,which are key sphingolipid(SL)species for lipid raft *** findings suggest thatα1-COP protein,together with SL modifiers controlling lipid raft compositions,regulates the subcellular localization of GPI-anchored PDBG2 proteins,and hence the callose turnover at PD and symplasmic movement of *** findings provide the first key clue to link the COPI-mediated intracellular trafficking pathway to the callose-mediated intercellular signaling pathway through PD.

关键词： callose coatomer proteins membrane-bound vesicle plasmodesmata sphingolipid enzymes

Decomposition kinetics of dimethyl methylphospate(chemical agent simulant) by supercritical water oxidation

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Journal of Environmental Sciences 2006年第1期18卷 13-16页

作者： Bambang VERIANSYAH jae-Duck KIM youn-Woo LEE Supercritical Fluid Research Laboratory Korea Institute of Science and Technology(KIST) 39-1 Hawolgok-dong Seoungbuk-gu Seoul 136-791 Korea. School of Chemical and Biological Engineering Seoul National University(SNU) San 56-1 Sillim-dong Gwanak-gu Seoul 151-744 Korea

Supercritical water oxidation （SCWO） has been drawing much attention due to effectively destroy a large variety of high-risk wastes resulting from munitions demilitarization and complex industrial chemical. An important design consideration in the development of supercritical water oxidation is the information of decomposition rate. In this paper, the decomposition rate of dimethyl methylphosphonate（DMMP）, which is similar to the nerve agent VX and GB（Sarin） in its structure, was investigated under SCWO conditions. The experiments were performed in an isothermal tubular reactor with a H2O2 as an oxidant. The reaction temperatures were ranged from 398 to 633℃ at a fixed pressure of 24 MPa. The conversion of DMMP was monitored by analyzing total organic carbon （TOC） on the liquid effluent samples. It is found that the oxidative decomposition of DMMP proceeded rapidly and a high TOC decomposition up to 99.99% was obtained within 11 s at 555℃. On the basis of data derived from experiments, a global kinetic equation for the decomposition of DMMP was developed. The model predictions agreed well with the experimental data.

关键词： supercritical water oxidation kinetics chemical agent DMMP

Improvement of uniformity in cultivation environment and crop growth rate by hybrid control of air flow devices

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Journal of Central South University 2015年第12期22卷 4702-4708页

作者： BAEK Min-Seon KWON Sook-youn LIM jae-Hyun Department of Computer Science and Engineering Kongju National University Green Energy Technology Research Center Kongju National University

A complete control type plant factory has high efficiency in terms of cultivation area by constructing vertical multiple layered cultivation ***,it has a problem of irregular crop growth due to temperature deviation at upper and lower beds and increases in energy consumption by a prolonged cultivation *** this work,air flow rate inside a facility was improved by a hybrid control of air flow devices like air conditioning and air circulation fan with an established wireless sensor network to minimize temperature deviations between upper and lower beds and to promote crop *** performance of proposed system was verified with an experimental environment or Case A wherein air conditioning device was operated without a control algorithm and Case B wherein air conditioning and circulation fans were alternatively operated based on the hybrid control *** planting leafy vegetables under each experimental condition,crops were cultivated for 21 *** a result,Case B wherein AC(air conditioning) and ACF(air-circulation fan) were alternatively operated based on the hybrid control algorithm showed that fresh mass,number of leaves,and leaf length for the crops grown were increased by 40.6%,41.1%,and 11.1%,respectively,compared to Case A.

关键词： air flow devices hybrid control cultivation environment uniformity growth rate

Improvement of light uniformity by lighting arrangement for standardized crop production

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Journal of Central South University 2014年第11期21卷 4311-4319页

作者： Lee Hwa-Soo Kwon Sook-youn Lim jae-Hyun Department of Computer Science and Engineering Kongju National UniversityCheonan 331-717 Korea

In a commercialized, fully artificial plant factory, artificial luminaire is arranged in a unified way using a general illumination theory, an actual measurement, or an empirical methodology. However, with these methods, lightings are implemented without considering specific optical characteristics of lighting or material characteristics of each component that constructs a cultivation system, resulting in an amount of light that becomes irregular. The amount of lighting is closely related with the growth and quality of crops, and the deviation between points where cultivated crops are located causes quality difference in the produced crops, thus impairing the economic feasibility of a plant factory. In this regard, a simulation to figure out an optimum lighting layout was performed. Arrangements based on the spectrum distribution of light source and reflector materials were implemented to ascertain the distance between lighting and height of lighting and gather information in the pre-treatment process to improve the uniformity of light in the plant cultivation system. Improvement of around 15% in light uniformity is achieved compared with the existing system after the simulation is carried out. This result would reduce the deviation in crop growth to make uniform quality crop production possible.

关键词： high-quality crop light uniformity improvement optimal lighting layout fully artificial plant factory Relux simulation

Development of genome engineering technologies in cattle: from random to specific

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Journal of Animal Science and Biotechnology 2018年第2期9卷 285-293页

作者： Soo-young Yum Ki-young youn Woo-jae Choi Goo Jang Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University Farm Animal Clinical Training and Research Center Institute of Green Bio Science Technology Seoul National University Emergence Center for Food-Medicine Personalized Therapy System Advanced Institutes of Convergence Technology Seoul National University College of Veterinary Medicine Seoul National University

The production of transgenic farm animals(e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into one-cell stage embryos(zygotes) followed by embryo transfer into a recipient was performed because of the ease of the ***, as this approach resulted in severe mosaicism and has a low efficiency, it is not typically employed in the cattle as priority, unlike in mice. To overcome the above issue with micro-injection in cattle, somatic cell nuclear transfer(SCNT) was introduced and successfully used to produce cloned livestock. The application of SCNT for the production of transgenic livestock represents a significant advancement, but its development speed is relatively slow because of abnormal reprogramming and low gene targeting efficiency. Recent genome editing technologies(e.g.,ZFN, TALEN, and CRISPR-Cas9) have been rapidly adapted for applications in cattle and great results have been achieved in several fields such as disease models and bioreactors. In the future, genome engineering technologies wil accelerate our understanding of genetic traits in bovine and wil be readily adapted for bio-medical applications in cattle.

关键词： Cattle CRISPR-Cas9 Genome engineering technologies Transgenesis Transposon

One-step method to enhance biotribological properties and biocompatibility of DLC coating by ion beam irradiation

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Friction 2022年第7期10卷 1114-1126页

作者： Yuzhen LIU Kelun ZHANG jae-Ho HAN youn-Hoo HWANG Shusheng XU Dae-Eun KIM Department of Mechanical Engineering Yonsei UniversitySeoul 03722Republic of Korea Department of Dermatology and Cutaneous Biology Research Institute Yonsei University College of MedicineSeoul 03722Republic of Korea State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou 730000China

A one-step method was developed to create a highly biocompatible micropatterned surface on a diamond-like carbon(DLC)through irradiation with a nitrogen ion beam and thus enhance the biocompatibility of osseointegrated surfaces and biotribological performance of articular *** biocompatibility and biotribological mechanisms were analyzed in terms of the structure and morphology of *** was demonstrated that a layer enriched in sp^(3) C-N bonds was formed on the surface of the DLC after nitrogen ion beam ***,with an increase in the radiation dose,the content of sp^(3) C-N on the DLC surface increased significantly,and the biocompatibility was positively correlated with *** adhesion of the MC3T3 osteoblasts increased significantly from 32%to 86%under an irradiation dose of 8×10^(15) ions/cm^(2).In contrast,the micropattern had a significant negative effect on the adhesion of the osteoblasts as it physically hindered cell expansion and *** micropattern with a depth of 37 nm exhibited good friction properties,and the coefficient of friction was reduced by 21%at relatively high speeds.

关键词： ion beam irradiation biocompatibility diamond-like carbon(DLC) in-vivo micropattern biotribological performance

Weekend and nighttime effect on the prognosis of peptic ulcer bleeding

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World Journal of Gastroenterology 2012年第27期18卷 3578-3584页

作者： young Hoon youn Yong Jin Park jae Hak Kim Tae Joo Jeon jae Hee Cho Hyojin Park Department of Internal Medicine Gangnam Severance HospitalYonsei University College of MedicineSeoul 135-720South Korea Department of Internal Medicine Dongguk University Ilsan HospitalDongguk University College of MedicineGyeonggi-do 410-773South Korea Department of Internal Medicine Inje University Sanggye Paik HospitalInje University College of MedicineSeoul 139-707South Korea Department of Internal Medicine Kwandong University Myungji HospitalKwandong University College of MedicineGyeonggi-do 412-270South Korea

AIM:To evaluate whether weekend or nighttime admission affects prognosis of peptic ulcer bleeding despite early ***:Retrospective data collection from four referral centers,all of which had a formal out-of-hours emergency endoscopy service,even at weekends.A total of 388 patients with bleeding peptic ulcers who were admitted via the emergency room between January 2007 and December 2009 were *** parameters included time from patients' arrival until endoscopy,mortality,rebleeding,need for surgery and length of hospital ***:The weekday and weekend admission groups comprised 326 and 62 patients,*** were no significant differences in baseline characteristics between the two groups,except for younger age in the weekend *** patients (97%) had undergone early endoscopy,which resulted in a low mortality rate regardless of point of presentation (1.8% overall vs 1.6% on the weekend).The only outcome that was worse in the weekend group was a higher rate of rebleeding (12% vs 21%,P = 0.030).However,multivariate analysis revealed nighttime admission and a high Rockall score (≥ 6) as significant independent risk factors for rebleeding,rather than weekend ***:Early endoscopy for peptic ulcer bleeding can prevent the weekend effect,and nighttime admission was identified as a novel risk factor for rebleeding,namely the nighttime effect.

关键词： Early endoscopy Nighttime effect Pepticulcer bleeding Rebleeding Weekend effect

Dilute H_2SO_4 solution for copper seed cleaning in electroplating

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Transactions of Nonferrous Metals Society of China 2013年第2期23卷 562-566页

作者： youn-Seoung LEE jae-Sik YOON Yang-Rae JO Heesoo LEE Sa-Kyun RHA Department of Information Communication Engineering Hanbat National University School of Materials Science and Engineering Pusan National University Department of Materials Science and Engineering Hanbat National University

The effects of surface cleaning to eliminate the surface oxides formed on Cu seed layer with dilute H2SO4 solution were investigated. Cu seed layer formed on Ti/Si（100） wafer by sputter deposition was exposed to air to grow native Cu oxide. Dilute H2SO4 solutions and/or TS-40A alkaline soak cleaner were used to remove the native Cu-oxide. After mainly carbon groups （such as C=O） on surface of Cu seed layer were removed by pretreatment of TS-40A alkaline solution, subsequently, dilute H2SO4 acid solution removed Cu-oxides （Cu20 and CuO） as well as a lot of O=C and Cu（OH）2.

关键词： pretreatment copper-oxide H2SO4 XPS sheet resistance

Copper nanowire/multi-walled carbon nanotube com- posites as all-nanowire flexible electrode for fast-charging/ discharging lithium-ion battery

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Nano Research 2018年第2期11卷 769-779页

作者： Zhenxing Yin Sanghun Cho Duck-jae You Yong-keon Ahn Jeeyoung Yoo youn Sang Kim Program in Nano Science and Technology Graduate School of Convergence Science and Technology Seoul National University Seoul Republic of Korea Advanced Institutes of Convergence Technology Gyeonggi-do Republic of Korea

A novel lightweight three-dimensional （3D） composite anode for a fast-charging] discharging Li-ion battery （LIB） was fabricated entirely using one-dimensional （1D） nanomaterials, i.e., Cu nanowires （CuNWs） and multi-walled C nanotubes （MWCNTs）. Because of the excellent electrical conductivity, high-aspect ratio structures, and large surface areas of these nanomaterials, the CuNW-MWCNT composite （CNMC） with 3D structure provides significant advantages regarding the transport pathways for both electrons and ions. As an advanced binder-free anode, a CuNW-MWCNT composite film with a controllable thickness （~ 600 prn） exhibited a considerably low sheet resistance, and internal cell resistance. Furthermore, the random CuNW network with 3D structure acting as a rigid framework not only prevented MWCNT shrinkage and expansion due to aggregation and swelling but also minimized the effect of the volume change during the charge/discharge process. Both a half cell and a full cell of LIBs with the CNMC anode exhibited high specific capacities and Coulombic efficiendes, even at a high current. More importantly, we for the first time overcame the limitation of MWCNTs as anode materials for fast-charging]discharging LIBs （both half cells and full cells） by employing CuNWs, and the resulting anode can be applied to flexible LIBs. This innovative anode structure can lead to the development of ultrafast chargeable LIBs for electric vehides.

关键词： three-dimensional (3D)composite,all-nanowire electrode,fast chargeable batter3 fuN-cell Li-ion batteries(L1Bs),flexible battery