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Light(Science & Applications) 2016年第1期5卷 303-312页

作者： Jiang-Lai wu yi-Qing Xu Jing-Jiang Xu Xiao-Ming wei Antony CS Chan Anson HL Tang Andy KS Lau Bob MF Chung Ho Cheung Shum Edmund y lam Kenneth Ky wong Kevin K Tsia Department of Electrical and Electronic Engineering The University of Hong KongPokfulam RoadHong Kong 999077China Department of Bioengineering University of WashingtonSeattleWashington 98195USA Department of Mechanical Engineering The University of Hong KongPokfulam RoadHong Kong 999077China

Optical time-stretch imaging enables the continuous capture of non-repetitive events in real time at a line-scan rate of tens of MHz—a distinct advantage for the ultrafast dynamics monitoring and high-throughput screening that are widely needed in biological ***,its potential is limited by the technical challenge of achieving significant pulse stretching(that is,high temporal dispersion)and low optical loss,which are the critical factors influencing imaging quality,in the visible spectrum demanded in many of these *** present a new pulse-stretching technique,termed free-space angular-chirpenhanced delay(FACED),with three distinguishing features absent in the prevailing dispersive-fiber-based implementations:(1)it generates substantial,reconfigurable temporal dispersion in free space(41 ns nm^(−1))with low intrinsic loss(o6 dB)at visible wavelengths;(2)its wavelength-invariant pulse-stretching operation introduces a new paradigm in time-stretch imaging,which can now be implemented both with and without spectral encoding;and(3)pulse stretching in FACED inherently provides an ultrafast all-optical laser-beam scanning mechanism at a line-scan rate of tens of *** FACED,we demonstrate not only ultrafast laser-scanning time-stretch imaging with superior bright-field image quality compared with previous work but also,for the first time,MHz fluorescence and colorized time-stretch *** results show that this technique could enable a wider scope of applications in high-speed and high-throughput biological microscopy that were once out of reach.

关键词： optical time-stretch imaging pulse stretching ultrafast laser scanning

Genomics-driven derivatization of the bioactive fungal sesterterpenoid variecolin:Creation of an unnatural analogue with improved anticancer properties

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Acta Pharmaceutica Sinica B 2024年第1期14卷 421-432页

作者： Dexiu yan Jemma Arakelyan Teng wan Ritvik Raina Tsz Ki Chan Dohyun Ahn Vladimir Kushnarev Tsz Kiu Cheung Ho Ching Chan Inseo Choi Pui yi Ho Feijun Hu yujeong Kim Hill lam Lau ying Lo Law Chi Seng Leung Chun yin Tong Kai Kap wong wing lam yim Nikolay S.Karnaukhov Richard y.C.Kong Maria V.Babak yudai Matsuda Department of Chemistry City University of Hong KongHong Kong SARChina iGEM Team“VarieCure” City University of Hong KongHong Kong SARChina Department of Biomedical Engineering City University of Hong KongHong Kong SARChina Moscow Clinical Research Center Named After A.S.Loginov Moscow 111123Russian Federation

A biosynthetic gene cluster for the bioactive fungal sesterterpenoids variecolin(1)and variecolactone(2)was identified in Aspergillus aculeatus ATCC *** production of 1 and 2 was achieved in Aspergillus oryzae by expressing the sesterterpene synthase VrcA and the cytochrome P450 ***,the replacement of VrcB with homologous P450s from other fungal terpenoid pathw.y. yielded three new variecolin analogues(5-7).Analysis of the compounds’anticancer activity in vitro and in vivo revealed that although 5 and 1 had comparable activities,5 was associated with significantly reduced toxic side effects in cancer-bearing mice,indicating its potentially broader therapeutic *** study describes the first tests of variecolin and its analogues in animals and demonstrates the utility of synthetic biology for creating molecules with improved biological activities.

关键词： Animal studies Anticancer properties Biosynthesis Natural products Sesterterpenoids Synthetic biology Terpene synthases Variecolin

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有限群的表示一百年（Ⅱ

数学译林 2002年第4期21卷 319-329,303页

作者： T.y.lam

Doxorubicin-loaded PLGA Microparticles with Internal Pores for Longacting Release in Pulmonary Tumor Inhalation Treatment

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Chinese Journal of Polymer Science 2015年第7期33卷 947-954页

作者： Tian-shi Feng 田华雨 Cai-na Xu Lin Lin Michael Hon-wah lam Hao-jun Liang Xue-si Chen CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of Sciences Department of Biology and Chemistry City University of Hong Kong Advanced Laboratory of Environmental Research and Technology(ALERT) Joint Advanced Research Center

Doxorubicin（DOX） loaded poly（lactic-co-glycolic acid）（PLGA） microparticles with internal pores（MP-D） were developed for long-acting release in pulmonary inhalation treatment. The PLGA microparticles exhibited favorable aerodynamic properties for pulmonary delivery. In vitro drug release profile suggested that MP-D have the advantage of long-term maintenance of drug concentrations. MTT assay demonstrated the in vitro anti-tumor efficiency of the DOX loaded PLGA microparticles. Furthermore, melanoma lung metastasis model was established to determine the in vivo antitumor efficiency. The mice treated with MP-D showed significantly fewer lesions than the untreated ones. The survival analysis indicated that MP-D prolonged the survival time of tumor-bearing mice. These results suggested that DOX loaded PLGA microparticles with internal pores have the potential to be used as long-acting release carriers in clinical lung cancer treatment.

关键词： Doxorubicin Poly(lactic-co-glycolic acid) Internal pores Long-acting release Pulmonary inhalation

Exendin-4 attenuates atherosclerosis progression via controlling hematopoietic stem/progenitor cell proliferation

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Journal of Molecular Cell Biology 2023年第2期15卷 43-57页

作者： Cen yan Xiaojuan Ma Sin Man lam yuejie Zhang yu Cao yuan Dong Li Su Guanghou Shui yingmei Feng Department of Science and Development Beĳing Youan hospitalCapital Medical UniversityBeĳing 100069China Center of Basic Medical Research Institute of Medical Innovation and ResearchPeking University Third HospitalBeĳing 100191China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeĳing 100101China Neuroscience Research Institute Peking University Center of Medical and Health AnalysisPeking UniversityBeĳing 100191China

Beyond glycemic control, applications of glucagon-like peptide-1 receptor (GLP-1r) agonists (GLP-1 RAs) inhibit inflammationand plaque development in murine atherosclerotic models. However, whether they modulate hematopoietic stem/progenitor cells(HSPCs)to prohibit skewed myelopoiesis in hypercholesteremia remains unknown. In this study, GLP-1r expression in fluorescenceactivated cell sorting (FACS)-sorted wild-type HSPCs was determined by capillary western blotting. Bone marrow cells (BMCs)of wild-type or GLP-1r−/− mice were transplanted into lethally irradiated low-density lipoprotein receptor deficient (LDLr−/−)recipients followed by high-fat diet (HFD) for chimerism analysis by FACS. In parallel, LDLr−/− mice were placed on HFD for 6weeks and then treated with saline or Exendin-4 (Ex-4) for another 6 weeks. HSPC frequency and cell cycle were analyzed byFACS, and intracellular metabolite levels were assessed by targeted metabolomics. The results demonstrated that HSPCs expressedGLP-1r and transplantation of GLP-1r−/− BMCs resulted in skewed myelopoiesis in hypercholesterolemic LDLr−/− *** vitro, Ex-4 treatment of FACS-purified HSPCs suppressed cell expansion and granulocyte production induced by LDL. In vivo, Ex-4treatment inhibited plaque progression, suppressed HSPC proliferation, and modified glycolytic and lipid metabolism in HSPCs ofhypercholesteremic LDLr−/− mice. In conclusion, Ex-4 could directly inhibit HSPC proliferation induced by hypercholesteremia.

关键词： glucagon-like peptide-1 receptor agonist hematopoietic stem/progenitor cells proliferation metabolomics atherosclerosis

Hybrid brightfield and darkfield transport of intensity approach for high-throughput quantitative phase microscopy

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Advanced Photonics 2022年第5期4卷 15-26页

作者： Linpeng Lu Jiaji Li yefeng Shu Jiasong Sun Jie Zhou Edmund y.lam Qian Chen Chao Zuo Nanjing University of Science and Technology Smart Computational Imaging Laboratory(SCILab)NanjingChina Nanjing University of Science and Technology School of Electronic and Optical EngineeringNanjingChina Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense NanjingChina Nanjing University of Science and Technology Smart Computational Imaging Research Institute(SCIRI)NanjingChina The University of Hong Kong Department of Electrical and Electronic EngineeringPokfulamHong KongChina

Transport of intensity equation(TIE)is a well-established non-interferometric phase retrieval approach that enables quantitative phase imaging(QPI)by simply measuring intensity images at multiple axially displaced *** advantage of a TIE-based QPI system is its compatibility with partially coherent illumination,which provides speckle-free imaging with resolution beyond the coherent diffraction ***,TIE is generally implemented with a brightfield(BF)configuration,and the maximum achievable imaging resolution is still limited to the incoherent diffraction limit(twice the coherent diffraction limit).It is desirable that TIE-related approaches can surpass this limit and achieve high-throughput[high-resolution and wide field of view(FOV)]*** propose a hybrid BF and darkfield transport of intensity(HBDTI)approach for highthroughput quantitative phase *** through-focus intensity stacks corresponding to BF and darkfield illuminations are acquired through a low-numerical-aperture(NA)objective *** high-resolution and large-FOV complex amplitude(both quantitative absorption and phase distributions)can then be synthesized based on an iterative phase retrieval algorithm taking the coherence model decomposition into *** effectiveness of the proposed method is experimentally verified by the retrieval of the USAF resolution target and different types of biological *** experimental results demonstrate that the half-width imaging resolution can be improved from 1230 nm to 488 nm with 2.5×expansion across a 4×FOV of 7.19 mm2,corresponding to a 6.25×increase in space-bandwidth product from∼5 to∼30.2 *** contrast to conventional TIE-based QPI methods where only BF illumination is used,the synthetic aperture process of HBDTI further incorporates darkfield illuminations to expand the accessible object frequency,thereby significantly extending the maximum available resolution from 2NA to∼5NA with a∼5×promotion of the coherent diffra

关键词： transport of intensity equation phase retrieval darkfield imaging high-throughput microscopy

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补血清肝四宝

心血管病防治知识 2011年第5期 8-10页

作者：张云辉 jacky.Z.y

温馨贴士甲鱼营养丰富,但腥昧较重,煲汤前,将甲鱼的胆汁与水混合,涂于甲鱼全身,稍等片刻,用清水把胆汁洗掉,可除去腥味。知母银耳甲鱼汤汤料佳配:知母、黄柏、天冬、女贞子各10克,银耳15克,甲鱼1只,生姜、葱各适量。煲汤程序:①知母、...

温馨贴士甲鱼营养丰富,但腥昧较重,煲汤前,将甲鱼的胆汁与水混合,涂于甲鱼全身,稍等片刻,用清水把胆汁洗掉,可除去腥味。知母银耳甲鱼汤汤料佳配:知母、黄柏、天冬、女贞子各10克,银耳15克,甲鱼1只,生姜、葱各适量。煲汤程序:①知母、黄柏、天冬、女贞子分别洗净,装入药袋。②银耳洗净,发水。③甲鱼杀好,洗净。④

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补血清肝四宝

心血管病防治知识 2011年第4期 8-9页

作者：张云辉 jacky.Z.y

知母银耳甲鱼汤汤料佳配：知母、黄柏、天冬、女贞子各lO克，银耳15克，甲鱼1只，生姜、葱各适量。

关键词：清肝补血甲鱼汤女贞子银耳知母

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健脾补肾汤

心血管病防治知识 2011年第4期 10-11页

作者：张云辉 jacky.Z.y

桂圆羊肉汤桂圆肉30克，羊肉500克，生姜30克，葱、盐、料酒各少许。

关键词：健脾补肾汤羊肉汤桂圆肉