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Promoter hypermethylation and loss of CD133 gene expression in colorectal cancers

World Journal of Gastroenterology 2010年第25期16卷 3153-3160页

作者： You-Kyung Jeon Sung-Hee Kim Seung-Ho Choi Kyung-Hee Kim Byong-Chul Yoo ja-lok ku jae-Gahb Park Laboratory of Cell Biology Cancer Research Institute Seoul National University College of Medicine Seoul 110-799 South Korea Colorectal Cancer Branch Research Institute National Cancer Center Goyang 411-764 Gyeonggi South Korea

AIM: To understand CD133 promoter hypermethyl-ation and expression in 32 colorectal cancer cell lines. METHODS: Nucleic acid was isolated from 32 colorectal cancer cell lines and CD133 expression levels were measured by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Promoter methylation status of the CD133 gene was analyzed with a methylation-specific PCR after sodium-bisulfi te modification and by clonal sequencing analysis. The correlation between expression and promoter methylation of CD133 gene was confirmed with treatment of 5-aza-2’-deoxycytidine. RESULTS: We measured CD133 expression levels in 32 colorectal cancer cell lines. RT-PCR analysis showed undetectable or low levels of CD133 expression in 34.4%of cell lines. To verify the relation between CD133 expression and methylation status of the CD133 gene promoter in colorectal carcinogenesis, CD133 gene promoter hypermethylation was analyzed in 32 cancer cell lines. Promoter hypermethylation was detected in 13 (40.6%) of the cell lines using methylation specificPCR and confirmed by bisulfite sequencing analysis. Treatment of 11 of the cell lines with the demethylation agent 5-aza-2’-deoxycytidine recovered CD133 expression in most of them. CONCLUSION: Transcriptional repression of CD133 is caused by promoter hypermethylation of the CD133 CpG islands in some of colorectal cancer cell lines. The study may contribute to the understanding of the role of CD133 inactivation in the progression of colorectal cancers.

关键词： CD133 Promoter Hypermethylation Colorectal cancer Sodium bisulfite modification

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The Mystery 40 Hz:Unraveling the Efficacy of Rhythmic Stimulation in Alzheimer's Disease

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Neuroscience Bulletin 2024年第6期40卷 831-834页

作者： Yixuan ku Guangdong Provincial Key Laboratory of Brain Function and Disease Center for Brain and Mental Well-beingDepartment of PsychologySun Yat-sen UniversityGuangzhou510006China Peng Cheng Laboratory Shenzhen518000China

Promises and Challenges of Rhythmic *** advances in research have made significant strides in understanding the potential of 40 Hz rhythmic optogenetic stimulation for the treatment of Alzheimer's disease(AD).A pivotal study conducted by Iaccarino et al.(2016)unveiled that targeted stimulation of interneurons at this specific frequency results in a remarkable reduction ofβ-amyloid levels in a mouse model of AD[1].

关键词： stimulation Alzheimer al.

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Nearly degenerate ground states of a checkerboard antiferromagnet and their bosonic interpretation

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Science China(Physics,Mechanics & Astronomy) 2024年第1期67卷 84-89页

作者： Haiyuan Zou Fan Yang Wei ku Key Laboratory of Polar Materials and Devices(MOE) School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China Department of Physics Beijing Institute of TechnologyBeijing 100081China Tsung-Dao Lee Institute Shanghai 200240China Key Laboratory of Artificial Structures and Quantum Control(Ministry of Education) Shanghai 200240China Shanghai Branch Hefei National LaboratoryShanghai 201315China

The spin-1/2 model system with antiferromagnetic(AF) couplings on a J1-J2checkerboard lattice, known as the planar pyrochlore model, is strongly frustrated and associated with a two-to-one dimensional crossover. Using the Projected Entangled Simplex States tensor network ansatz, we identify a large number of nearly degenerate states in the frustrated region(J_(1)

关键词： tensor networks frustrated magnetism dimensional crossover degenerate states

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The Sequential Pricing of Ride-Hailing System with Rental Service in the Context of Fleet Electrification

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Journal of Systems Science and Systems Engineering 2024年第1期33卷 77-105页

作者： Yaoyao ku Peng Wu Qiang Ren Yiqing Wang Business School Sichuan UniversityChengdu610065China

Surging demand and reduced capacity in the ride-hailing industry have prompted numerous ride-hailing platforms to build their own car-rental services catering to drivers who do not possess private vehicles. However, the trade-off between the ride-hailing service and the car-rental service is an important issue that is still unclear in theory. Moreover, ride-hailing platforms are transitioning towards all-electric fleets in the context of Carbon Neutrality goals and government regulations. This paper considers a ride-hailing system comprising a monopolist ride-hailing platform, an electric vehicle (EV) rental firm, and a gasoline vehicle (GV) rental firm. Furthermore, we build a stylized model to study the sequential pricing of the system. The equilibrium outcomes show the significant impact of the ride-hailing platform’s decision to continue or withdraw offering EV rental services on EV and GV drivers’ net earnings, rental prices, and wages. The ride-hailing platform providing EV rental services increases EV drivers’ net earnings but decreases the GV driver wages and rental prices. However, the EV rental service offered by the ride-hailing platform does not necessarily lead to an increased total profit for the system. The improved profitability of the system by the ride-hailing platform providing EV rental services is contingent upon lower rider prices and higher fuel costs. The ride-hailing platform’s EV rental services provision also effectively fosters the ride-hailing fleet’s electrification. Furthermore, as the number of riders increases, the ride-hailing platform should reduce the commission rate for EV drivers to maintain competitiveness and profitability.

关键词： Ride-hailing system car rental fleet electrification sequential pricing differential wage scheme

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Presence of CCK-A, B receptors and effect of gastrin and cholecystokinin on growth of pancreatobiliary cancer cell lines

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World Journal of Gastroenterology 2005年第6期11卷 803-809页

作者： Jin-Young jang Sun-Whe Kim ja-lok ku Yong-Hyun Park jae-Gahb Park Department of Surgery

AIM: To investigate the effects of gastrin and cholecystokin in(CCK) and their specific antagonists on the growth of pancreatic and biliary tract cancer cell ***: Five pancreatic and 6 biliary cancer cell lines with 2 conrtol cells were used in this study. Cell proliferation study was done using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MI-T) test and direct cell count method. Reverse transcription-polymerase chain reaction (RT-PCR) and slot blot hybridization were performed to examine and quantify the expression of hormonal receptors in these cell ***: SNU-308 showed a growth stimulating effect by gastrin-17, as did SNU-478 by both gastrin-17 and CCK-8. The trophic effect of these two hormones was completely blocked by specific antagonists (L-365, 260 for gastrin and L-364, 718 for CCK). Other cell lines did not respond to gastrin or CCK. In RT-PCR, the presence of CCK-A receptor and CCK-B/gastrin receptor mRNA was detected in all biliary and pancreatic cancer cell lines. In slot blot hybridization, compared to the cell lines which did not respond to hormones, those that responded to hormones showed high expression of receptor ***: Gastrin and CCK exert a trophic action on some of the biliary tract cancers.

关键词： CCK-A B受体胃泌激素肠促胰酶肽胰腺癌肿瘤消化系统

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Promoter hypomethylation and reactivation of MAGE-A1 and MAGE-A3 genes in colorectal cancer cell lines and cancer tissues

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World Journal of Gastroenterology 2006年第35期12卷 5651-5657页

作者： Kyung-Hee Kim Jin-Sung Choi Il-Jin Kim ja-lok ku jae-Gahb Park Laboratory of Cell Biology Korean Cell Line Bank Cancer Research Institute Seoul National University College of Medicine Seoul 110-744 Korea Laboratory of Cell Biology Korean Cell Line Bank Cancer Research Institute Seoul National University College of Medicine Seoul 110-744

AIM: To verify the expression and methylation status o the MAGE-A1 and MAGE-A3 genes in colorectal cance tissues and cancer cell lines. METHODS: We evaluated promoter demethylation status of the MAGE-A1 and MAGE-A3 genes by RT-PCR analysis and methylation-specific PCR (MS-PCR), as we as sequencing analysis, after sodium bisulfite modifica tion in 32 colorectal cancer cell lines and 87 cancer tis sues. RESULTS: Of the 32 cell lines, MAGE-A1 and MAGE-A3 expressions were observed in 59% and 66%, respective ly. Subsequent to sodium bisulfite modification and MS PCR analysis, the promoter hypomethylation of MAGE-A1 and MAGE-A3 was confirmed in both at 81% each Promoter hypomethylation of MAGE-A1 and MAGE-A3 in colorectal cancer tissues was observed in 43% and 77% respectively. Hypomethylation of MAGE-A1 and MAGE-A3 genes in corresponding normal tissues were observed in 2% and 6%, respectively. CONCLUSION: The promoter hypomethylation of MAGE genes up-regulates its expression in colorectal carcino mas as well as in gastric cancers and might play a signif icant role in the development and progression of human colorectal carcinomas.

关键词： MAGE-A1 MAGE-A3 细胞活化结直肠癌肿瘤细胞肿瘤组织

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Establishment and characterization of four human hepatocellular carcinoma cell lines containing hepatitis B virus DNA

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World Journal of Gastroenterology 1999年第4期5卷 17-23页

作者： jae Ho Lee 1, ja lok ku 1, Young Jin Park 1,2 , kuhn Uk Lee 2, Woo Ho Kim 3 and jae Gahb Park 1,2

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ＩＮＴＲＯＤＵＣＴＩＯＮＨｅｐａｔｏｃｅｌｕｌａｒｃａｒｃｉｎｏｍａ（ＨＣＣ）ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｐｒｅｖａｌｅｎｔｍａｌｉｇｎａｎｔｄｉｓｅａｓｅｓｅｎｃｏｕｎｔｅｒｅｄｉｎｔｈｅｗｏｒｌｄ，ｋｉｌｉｎｇｕｐｔｏ１ｍｉｌｉｏｎｐｅｏｐｌｅａ...

关键词： carcinoma, hepatocellular liver neoplasms hepatitis B virus hepatitis x antigen cell line

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Identification of thermal front dynamics in the northern Malacca Strait using ROMS 3D-model

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Journal of Oceanology and Limnology 2024年第1期42卷 41-57页

作者： ku Nor Afiza Asnida ku MANSOR Nur Hidayah ROSELI Poh Heng KOK Fariz Syafiq Mohamad ALI Mohd Fadzil Mohd AKHIR Institute of Oceanography and Environment Universiti Malaysia TerengganuKuala Nerus 21030Malaysia Faculty of Science and Marine Environment Universiti Malaysia TerengganuKuala Nerus 21030Malaysia

The thermal front in the oceanic system is believed to have a significant effect on biological *** an era of climate change,changes in heat regulation between the atmosphere and oceanic interior can alter the characteristics of this important *** the simulation results of the 3D Regional Ocean Modelling System(ROMS),we identified the location of thermal fronts and determined their dynamic variability in the area between the southern Andaman Sea and northern Malacca *** Single Image Edge Detection(SIED)algorithm was used to detect the thermal front from model-derived *** show that a thermal front occurred every year from 2002 to 2012 with the temperature gradient at the location of the front was 0.3°C/*** to the years affected by El Ni?o and negative Indian Ocean Dipole(IOD),the normal years(e.g.,May 2003)show the presence of the thermal front at every selected depth(10,25,50,and 75 m),whereas El Ni?o and negative IOD during 2010 show the presence of the thermal front only at depth of 75 m due to greater warming,leading to the thermocline deepening and enhanced *** May 2003,the thermal front was separated by cooler SST in the southern Andaman Sea and warmer SST in the northern Malacca *** higher SST in the northern Malacca Strait was believed due to the besieged Malacca Strait,which trapped the heat and make it difficult to release while higher chlorophyll a in Malacca Strait is due to the freshwater conduit from nearby rivers(Klang,Langat,Perak,and Selangor).Furthermore,compared to the southern Andaman Sea,the chlorophyll a in the northern Malacca Strait is easier to reach the surface area due to the shallower thermocline,which allows nutrients in the area to reach the surface faster.

关键词： regional ocean modelling system thermal front Andaman Sea Malacca Strait single image edge detection algorithm

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Numerical Simulation on Radon Retardation Behavior of Covering Floats in Radon-Containing Water

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Biomedical and Environmental Sciences 2024年第4期37卷 406-417页

作者： LIU Shu Yuan ZHANG Li YE Yong Jun DING ku Ke National Institute for Radiological Protection Chinese Center for Disease Control and PreventionBeijing 100088China Office for Public Health Management Chinese Center for Disease Control and PreventionBeijing 102206China Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy University of South ChinaHengyang 421001HunanChina

Objective This study aimed to efficiently reduce the release of radon from water bodies to protect the *** Based on the sizes of the experimental setup and modular float,computational fluid dynamics(CFD)was used to assess the impact of the area coverage rate,immersion depth,diffusion coefficient,and radon transfer velocity at the gas–liquid interface on radon migration and exhalation of radon-containing *** on the numerical simulation results,an estimation model for the radon retardation rate was *** effectiveness of the CFD simulation was evaluated by comparing the experimental and simulated variation values of the radon retardation rate with the coverage area *** The effect of radon transfer velocity on radon retardation in water bodies was minor and insignificant according to the appropriate value;therefore,an estimation model of the radon retardation rate of the coverage of a radon-containing water body was constructed using the synergistic impacts of three factors:area coverage rate,immersion depth,and diffusion *** deviation between the experimental and simulated results was<4.3%.Conclusion Based on the numerical simulation conditions,an estimation model of the radon retardation rate of covering floats in water bodies under the synergistic effect of multiple factors was obtained,which provides a reference for designing covering floats for radon retardation in radoncontaining water.

关键词： Radon-containing water Radon retardation rate CFD Coverage experiment Optimized design

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Review of tribological properties of nanoparticle-based lubricants and their hybrids and composites

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Friction 2024年第4期12卷 569-590页

作者： Ye Zar Ni HTWE Aws.S.AL-jaNABI Yasmin WADZER Hussin MAMAT ku NOORYASMIN School of Materials&Mineral Resources Engineering Universiti Sains Malaysia(Engineering Campus)Nibong Tebal 14300Malaysia School of Aerospace Engineering Universiti Sains Malaysia(Engineering Campus)Nibong Tebal 14300Malaysia Universiti Sains Malaysia

Due to their encouraging results,nanolubricants have been revolutionary in the field of *** degree,to which the new material may improve the tribology,energy savings,and durability,is a crucial consideration for any new additive to a conventional *** results of the earlier research on carbon,metal,metal oxide,and their composites and hybrid nanolubricants as well as their effects on tribology,are summarized in this review *** most popular measuring methodologies,the tribology results for lubricants with an oil base,biodegradable base,and a water base,as well as the reasons that explain these tribological advancements,are all included in this ***,prospects for more study in this area are emphasized.

关键词： nanolubricants tribology carbon metal metal oxide hybrid

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