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皖产桐桔梗提取物大鼠体内代谢产物鉴定及代谢途径分析

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中国中药杂志 2024年第14期49卷 3912-3923页

作者：王晶晶常相伟汪鑫蕊智楠楠王举涛查良平张小波蒋超桂双英安徽中医药大学药学院安徽合肥230012 省部共建安徽道地中药材品质提升协同创新中心安徽合肥230012 安徽省中医药科学院药物制剂研究所安徽合肥230012 药物制剂技术与应用安徽省重点实验室安徽合肥230012 安徽省道地药材品质提升与开发利用工程研究中心安徽合肥230012 中国中医科学院中药资源中心道地药材品质保障与资源持续利用全国重点实验室北京100700

研究桐桔梗在大鼠血浆、尿液、粪便中的原型成分和代谢产物,揭示其体内代谢途径及代谢规律。采用超高效液相色谱-串联四极杆飞行时间质谱(UPLC-q-TOF-MS)及质量亏损过滤(MDF)技术对桐桔梗在大鼠体内的原型成分及代谢产物进行系统表征与... 详细信息

研究桐桔梗在大鼠血浆、尿液、粪便中的原型成分和代谢产物,揭示其体内代谢途径及代谢规律。采用超高效液相色谱-串联四极杆飞行时间质谱(UPLC-q-TOF-MS)及质量亏损过滤(MDF)技术对桐桔梗在大鼠体内的原型成分及代谢产物进行系统表征与辨识。采用ACqUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),以0.1%甲酸水(A)-0.1%甲酸乙腈(B)为流动相进行梯度洗脱,在电喷雾质谱负离子模式下对生物样品进行分析。从灌胃给予桐桔梗提取物的大鼠血浆、尿液、粪便中共检测到12个皂苷类原型成分和27个代谢产物,其中血浆中共检测到11个原型成分和9个代谢产物,尿液中共检测到11个原型成分和8个代谢产物,粪便中共检测到10个原型成分和20个代谢产物。进一步研究表明,桐桔梗在大鼠体内的代谢途径主要包括氧化、还原、乙酰化、逐级水解脱糖、葡萄糖醛酸化等。该研究为阐明桐桔梗药效物质基础及作用机制提供了科学依据。

关键词：桐桔梗三萜皂苷原型成分代谢产物超高效液相色谱-串联四极杆飞行时间质谱

High-energy,alignment-insensitive,injection-seeded q-switched Ho:yttrium aluminum garnet single-frequency laser

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High Power Laser Science and Engineering 2023年第5期11卷 157-163页

作者： Dong Yan Yue Yuan Yunpeng Wang Jiawei Fan Jiaze Wu Xiaoming Duan Sining Li Tongyu Dai Youlun ju National Key Laboratory of Tunable Laser Technology Harbin Institute of TechnologyHarbinChina Department of Physics and Chemistry PLA Army Academy of Special OperationsGuangzhouChina Institute of Laser Engineering Faculty of Materials and ManufacturingBeijing University of TechnologyBeijingChina

A high-energy,alignment-insensitive,injection-seeded q-switched Ho:yttrium aluminum garnet(YAG)singlefrequency laser is *** the slave q-switched laser and the seed laser are Ho:YAG ring lasers based on a pair of corner cubic *** seed laser has an available power of 830 mW at 2096.667 *** 100 Hz,the q-switched Ho:YAG laser provides a single-frequency pulsed output using injection-seeded *** 7.3 mJ single-frequency pulse energy from the slave laser has a pulse width of 161.2 ns and is scaled to 33.3 mJ after passing through the Ho:YAG single-pass *** to the measurement results of the heterodyne beating technique,the single-frequency pulse has a half-width of 4.12 MHz.

关键词： alignment insensitivity corner cubic reflector high energy injection seeding single frequency

Soil Nematode Response to Biochar Addition in a Chinese Wheat Field

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Pedosphere 2013年第1期23卷 98-103页

作者： ZHANG Xiao-Ke LI qi LIANG Wen-ju ZHANG Min BAO Xue-Lian XIE Zu-Bin State Key Laboratory of Forest and Soil Ecology Institute of Applied Ecology Chinese Academy of Sciences University of Chinese Academy of Sciences Jiangsu Biochar Engineering Center State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences

While studies have focused on the use of biochar as soil amendment, little attention has been paid to its effect on soil fauna. The biochar was produced from slow pyrolysis of wheat straw in the present study. Four treatments, no addition (CK) and three rates of biochar addition at 2 400 (B1), 12 000 (B5) and 48 000 kg ha-1 (B20), were investigated to assess the effect of biochar addition to soil on nematode abundance and diversity in a microcosm trial in China. The B5 and B20 application significantly increased the total organic carbon and the C/N ratio. No significant difference in total nematode abundance was found among the treatments. The biochar addition to the soil significantly increased the abundance of fungivores, and decreased that of plant parasites. The diversity of soil nematodes was significantly increased by B1 compared to CK. Nematode trophic groups were more effectively indicative to biochar addition than total abundance.

关键词： diversity ecological indices microcosm trial nematode community trophic groups

磷酸肌酸钠联合维生素C治疗小儿病毒性心肌炎的效果

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中国合理用药探索 2024年第6期21卷 87-93页

作者：郝海菊王军张俊清蒋辉刘仿锦郑州大学第三附属医院小儿心血管内科郑州450052 郑州大学第三附属医院小儿内科郑州450052 郑州大学第三附属医院医务部郑州450052

目的:观察磷酸肌酸钠联合维生素C治疗小儿病毒性心肌炎的效果。方法:选取2020年5月~2023年12月某院收治的病毒性心肌炎患儿106例,采用信封法分为对照组和观察组,每组53例。对照组给予利巴韦林注射液、辅酶q10等常规治疗,观察组在常规治... 详细信息

目的:观察磷酸肌酸钠联合维生素C治疗小儿病毒性心肌炎的效果。方法:选取2020年5月~2023年12月某院收治的病毒性心肌炎患儿106例,采用信封法分为对照组和观察组,每组53例。对照组给予利巴韦林注射液、辅酶q10等常规治疗,观察组在常规治疗基础上给予磷酸肌酸钠联合维生素C治疗。比较治疗前后两组患儿心肌酶指标、心功能指标、单核细胞趋化蛋白-1(MCP-1)、高迁移率族蛋白B1(HMGB1)等因素水平,比较两组患儿临床疗效和安全性。结果:治疗后,两组患儿乳酸脱氢酶(LDH)、肌酸激酶同工酶(CK-MB)、羟丁酸脱氢酶(HBDH)、肌酸激酶(CK)、心肌肌钙蛋白I(cTnI)、MCP-1、HMGB1、正五聚蛋白-3(PTX-3)、细胞间黏附分子-1(ICAM-1)、超敏C反应蛋白(hs-CRP)、白介素-18(IL-18)均降低,且观察组低于对照组(P0.05);观察组总有效率(90.57%)高于对照组(75.00%,P<0.05);两组患儿不良反应总发生率比较无统计学差异。结论:磷酸肌酸钠与维生素C联合治疗小儿病毒性心肌炎可改善心肌酶、MCP-1、HMGB1等相关指标水平,提高临床疗效,且不会增加不良反应。

关键词：小儿病毒性心肌炎磷酸肌酸钠维生素C 心肌酶疗效

Nontoxic virus nanofibers improve the detection sensitivity for the anti-p53 antibody, a biomarker in cancer patients

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Nano Research 2015年第11期8卷 3562-3570页

作者： Pengtao Pan Yicun Wang Ye Zhu Xiang Gao Zhigang ju Penghe qiu Li Wang Chuanbin Mao Institute of Genetics and Cytology School of Life Sciences Northeast Normal University 5268 Renmin Street Changchun 130024 China Department of Chemistry and Biochemistry Stephenson Life Sciences Research Center University of Oklahoma Norman Oklahoma 73019 USA

The presence of anti-p53 antibody in serum is a biomarker for cancer. However, its high sensitivity detection is still an issue in cancer diagnosis. To tackle this challenge, we used fd phage, a human-safe bacteria-specific virus nanofiber that can be mass-produced by infecting host bacteria in an error-free manner, and genetically engineered it to display a peptide capable of recognizing and capturing anti-p53 antibody on its side wall. We employed the resultant phage nanofibers as a capture probe to develop a modified version of the enzyme- linked immunosorbent assay （ELISA） method, termed phage-ELISA. We compared it to the traditional ELISA method for the detection of anti-p53 antibody, p53-ELISA, which uses recombinant wild-type p53 protein to capture anti-p53 antibody. We applied phage-ELISA to detect anti-p53 antibody in an experimental group of 316 patients with various types of malignant tumors. We found that a detection rate of 17.7% （56 positive cases） was achieved by phage-ELISA, which was comparable to the detection rate of 20.6% for p53-ELISA （65 positive cases）. However, when both phage and p53 were combined to form antibody-capturing probes for phage/p53-ELISA, a detection rate of 30.4% （96 positive cases） was achieved. Our work showed that owing to the combined capture of the anti-p53 antibody by both phage nanofibers and p53, the phage/p53-ELISA achieved the highest diagnostic accuracy and detection efficiency for the anti-p53 antibody in patients with various types of cancers. Our work suggests that a combination of nanofibers and antigens, both of which capture antibody, could lead to increased detection sensitivity, which is useful for applications in the life sciences, clinical medicine, and environmental sciences.

关键词： phage virus protein nanofibers cancer diagnosis

Mutant p53 increases exosome-mediated transfer of mi R-21-3p and mi R-769-3p to promote pulmonary metastasis

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Chinese Journal of Cancer Research 2019年第3期31卷 533-546页

作者： qiang ju Lina Zhao Jiajia Gao Lanping Zhou Yang Xu Yulin Sun Xiaohang Zhao State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College

Objective: Tumor metastasis is a complex, multistep process that depends on tumor cells and their communication with the tumor microenvironment. A p53 gain-of-function mutant has been shown to enhance the tumorigenesis, invasion, and metastasis abilities of tumor cells. This study aimed to investigate the roles of p53 R273 H mutation in the tumor ***: The in vitro and in vivo effects of the p53 R273 H mutant on the invasion and metastasis of HCT116 cells were investigated. Exosomes from wild-type and HCT116-TP53(R273 H) cells were cocultured with mouse embryonic fibroblasts(MEFs). The roles of differentially expressed exosomal micro RNAs identified by microarray analysis were investigated. The functions of the p53 R273 H mutant in tumor cells were also investigated via gene expression microarray and quantitative polymerase chain reaction(q PCR) ***: Introducing p53 R273 H mutant into HCT116 cells significantly potentiated pulmonary metastasis in vivo. In the presence of exosomes derived from HCT116-TP53(R273 H) cells, the exosomes were taken up by MEFs and became activated. Microarray analysis showed that the p53 R273 H mutation increased the exosomal levels of mi R-21-3 p and mi R-769-3 p. Intriguingly, in clinical samples, mi R-21-3 p and mi R-769-3 p levels were significantly higher in patients with a p53 mutation than in those without this mutation. Furthermore, both mi R-21-3 p and mi R-769-3 p activated fibroblasts and exerted a synergistic effect via their target genes on the transforming growth factor-β(TGF-β)/Smad signaling pathway. The activated fibroblasts excreted cytokine TGF-β and may have reciprocally induced cancer cells to undergo epithelial-mesenchymal transition(EMT). Indeed, HCT116-TP53(R273 H) cells showed increased expression of ZEB1 and SNAI2 and decreased transcription of several cell adhesion ***: The mutant p53-exosomal mi R-21-3 p/mi R-769-3 p-fibroblast-cytokine circuit appears to be res

关键词： miR-21-3p miR-769-3p exosome tumor metastasis tumor microenvironment

Inhibition of long non-coding RNA TUG1 on gastric cancer cell transference and invasion through regulating and controlling the expression of miR-144/c-Met axis

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Asian Pacific Journal of Tropical Medicine 2016年第5期9卷 494-498页

作者： Ting-Ting Ji Xuan Huang Jie Jin Sheng-Hua Pan Xiao-ju Zhuge Department of Gastroenterology the Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital) Department of Gastroenterology Zhejiang Provincial Hospital of Chinese Traditional Medicine Department of Gastroenterology Wenzhou Central Hospital Department of Pathology the Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital)

Objective: To discuss the expression of long noncoding RNA TUG1 (IneRNA-TUG1) in gastric carcinoma (GC) and its effects on the transferring and invading capacity of gastric carcinoma cells. Methods: Forty cases of carcinoma tissue and para-carcinoma tissue were selected from GC patients who underwent surgical removal in Zhejiang Provincial Hospital of Chinese Traditional Medicine and Wenzhou Central Hospital from January, 2013 to December, 2014; the expressing level of IncRNA-TUG I in GC and para-C tissues was detected by applying the qRT-PCR technique. The correlation between IncRNA-TUG1 expression and patients' clinical data was classified and analyzed. SGC-7901 cells were transfected using IncRNA-TUG1 specific siRNA. Changes of the transferring and invading capacity of siRNA-transfected SGC-7901 cells were scratch-tested and transwell-detected. qRT-PCR was applied to detect the expression level of microRNA-144 after IncRNA-TUG1 was silenced. Changes of c-Met mRNA and protein expressions was detected by qRT-PCR and western -blot test. Results: The expression level of lneRNA-TUG1 in GC tissue was significant higher than that in para-C tissue (PqRT-PCR and western -blot proved that the expression of microRNA-144 was significantly boosted and the expression level of c-Met mRNA and protein was inhibited after IncRNA-TUG1 was silenced (P<0.05). Conclusions: IncRNA-TUG1 shows an up -regulated expression in GC tissue and that bears a correlation with clinicopathological features of malignant tumor. IncRNATUGI can promote the transferring and invading capacity of GC by inhibiting the pathway of microRNA-144/c-Met.

关键词： lneRNA-TUG1 Gastric carcinoma Transference Invasion microRNA-144 c-Met

A robust graphene oxide memristor enabled by organic pyridinium intercalation for artificial biosynapse application

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Nano Research 2023年第8期16卷 11278-11287页

作者： Yang Li Songtao Ling Ruiyu He Cheng Zhang Yue Dong Chunlan Ma Yucheng Jiang ju Gao Jinghui He qichun Zhang Jiangsu Key Laboratory of Micro and Nano Heat Fluid Flow Technology and Energy Application School of Physical Science and TechnologySuzhou University of Science and TechnologySuzhou 215009China College of Chemistry Chemical Engineeringand Materials ScienceSoochow UniversitySuzhou 215123China Department of Materials Science and Engineering City University of Hong KongKowloonHong Kong 999077China Department of Chemistry&Center of Super-Diamond and Advanced Films(COSDAF) City University of Hong KongKowloonHong Kong 999077China

Graphene oxide(GO)-based memristors offer the promise of low cost,eco-friendliness,and mechanical flexibility,making them attractive candidates for outstanding flexible electronic ***,their resistive transitions often display abrupt change rather than bidirectional progressive tuning,which largely limits their applications for biological synapse emulation and neuromorphic ***,a memristor with a novel layered structure of GO/pyridinium/GO is presented with tunable bidirectional *** inserted organic pyridinium intercalation succeeds in serving as a satisfactory buffer layer to intrinsically control the formation of conductive filaments during device operation,leading to progressive conductance ***,the essential synaptic behaviors including analog memory characteristics,excitatory postsynaptic current,paired pulse facilitation,prepulse inhibition,spike-timing-dependent plasticity,and spike-rate-dependent plasticity are *** emulation of brain-like“learning-forgetting-relearning”process is also ***,the instant responses of the memristor can be stimulated by low operational voltages and short pulse *** study paves one way for GO-based memristors to actuate appealing features such as bidirectional tuning and fast speed switching that are desirable for the development of bio-inspired neuromorphic systems.

关键词： graphene oxide nanoscale memristor 2D materials flexible electronics artificial synapses

Late Pleistocene aeolian activity in Haitan Island, Southeast China: Insights from optically stimulated luminescence dating of coastal dunes on marine terraces

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Journal of Mountain Science 2018年第8期15卷 1777-1788页

作者： JIN Jian-hui LI Zhi-zhong CHENG Yan XU Xiao-lin LI Zhi-xing LIU Xiao-ju Institute of Geography Fujian Normal University Key Laboratory for Humid Subtropical Eco-geographical Processes of the Ministry of Education Fujian Normal University College of Geographical Sciences Fujian Normal University

The 'Old Red Sand' is a type of semicemented medium-fine sandy sediment that is red(10R_4/8) or brown red(2.5YR_4/8) in colour and is found in late quaternary deposits. The sediments have distinctive characteristics and are a critical archive for understanding climatic changes in the coastal areas of East Asia. The ages of the late quaternary aeolian sand dunes from Haitan Island in the coastal area of South China are still in debate. In this study, three sets of marine terraces were identified in the northern region of Haitan Island. Aeolian dune sands are well preserved on the top of these terraces. quartz Optically Stimulated Luminescence dating and the distribution of the formation ages demonstrated that the palaeo-dunes are deposits from the middle-late period of the Late Pleistocene(q_3^(2-3)). The period may be divided into three stages, 100-90 ka, 70-60 ka, and 40-20 ka, in which the palaeo-dunes of the first two stages are more widespread and were formed separately during a low-sea level period of the Marine Isotope Stages 5 b and 4. Several depositional palaeo-flood event records were preserved during the last stage due to the increasing gradient of mountain gullies formed during the Last Glacial Maximum.

关键词： Aeolian activity Sediment dating Optically Stimulated Luminescence (OSL) Late Pleistocene Environmental evolution Haitan Island