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Rapid identification of full-length genome and tracing variations of monkeypox virus in clinical specimens based on mNGS and amplicon sequencing

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Virologica Sinica 2024年第1期39卷 134-143页

作者： Changcheng Wu Ruhan A Sheng Ye Fei Ye Weibang Huo Roujian Lu Yue Tang jianwei Yang Xuehong Meng Yun Tang Shuang Chen Li z.ao Baoying Huang z.ongxian z.ang Yuda Chen Dongfang Li Wenling Wang Ke-jia Shan jian Lu Wenjie Tan NHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing100052China Chongqing Municipal Key Laboratory for High Pathogenic Microbes Chongqing Center for Disease Control and PreventionChongqing400042China MGI BGI-ShenzhenShenzhen518083China Thermo Fisher Scientific Beijing100013China School of Public Health Baotou Medical CollegeBaotou014030China BGI PathoGenesis Pharmaceutical Technology Shenzhen518000China State Key Laboratory of Protein and Plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijing100871China College of Life Sciences University of Chinese Academy of SciencesBeijing100049China

The monkeypox virus(MPXV)has triggered a current outbreak *** sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and *** is a significant challenge but necessary to optimiz. the strategy and application of rapid full-length genome identification and to track variations of MPXV in clinical specimens with low viral loads,as it is one of the DNA viruses with the largest genome and the most AT-biased,and has a significant number of tandem *** we evaluated the performance of metagenomic and amplicon sequencing techniques,and three sequencing platforms in MPXV genome sequencing based on multiple clinical specimens of five mpox cases in Chinese *** rapidly identified the full-length genome of MPXV with the assembly of accurate tandem repeats in multiple clinical *** sequencing enables cost-effective and rapid sequencing of clinical specimens to obtain high-quality MPXV ***-generation sequencing facilitates the assembly of the terminal tandem repeat regions in the monkeypox virus genome and corrects a common misassembly in published ***,several intra-host single nucleotide variations were identified in the first imported mpox *** study offers an evaluation of various strategies aimed at identifying the complete genome of MPXV in clinical *** findings of this study will significantly enhance the surveillance of MPXV.

关键词： Monkeypox virus(MPXV) Metagenomic Next generation sequencing Amplicon Third-generation sequencing

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基于近场光学成像技术的极化激元学研究进展

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物理学报 2024年第8期73卷 1-21页

作者：周怡汐李志鹏陈佳宁首都师范大学物理系北京100048 中国科学院物理研究所北京100190 中国科学院大学物理学院北京100049 北京凝聚态物理国家研究中心北京100190 松山湖材料实验室东莞523808

极化激元作为光与物质的混合激发可以实现纳米光场的精确调控,为未来纳米光电器件的小型化和集成化提供了有效的途径.近年来,借助散射型扫描近场光学显微镜对多类型极化激元的观测,多种光学现象背后的物理机制被揭示,进一步加深了对极... 详细信息

极化激元作为光与物质的混合激发可以实现纳米光场的精确调控,为未来纳米光电器件的小型化和集成化提供了有效的途径.近年来,借助散射型扫描近场光学显微镜对多类型极化激元的观测,多种光学现象背后的物理机制被揭示,进一步加深了对极化激元物理和相互作用的理解,也极大地推动了极化激元调控及其应用的研究.基于此,本文总结了最新的极化激元近场研究进展.不同于前期关于二维材料极化激元的综述,本文不仅涵盖了三维至一维的极化激元材料体系,还在极化激元纳米光学特性方面增添了各向异性极化激元的最新研究工作,并且系统总结了极化激元调控的最新进展以及在亚衍射成像和聚焦、纳米结构识别、光调制器和分子检测等方面的相关应用.最后,对极化激元未来的研究方向进行了展望.

关键词：近场光学成像极化激元各向异性调控及应用

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Molecular glue triggers degradation of PHGDH by enhancing the interaction between DDB1 and PHGDH

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ACTA PHARMACEUTICA SINICA B 2024年第9期14卷 4001-4013页

作者： Huang, z.qi z.ang, Kun jiang, Yurui Wang, Mengmeng Li, Mei Guo, Yuda Gao, Ruolin Li, Ning Wang, Chenyang Chen, jia Wang, Jiefu Liu, Ning Liu, Xiang Liu, Shuangwei Wei, Mingming Yang, Cheng Yang, Guang Nankai Univ Coll Life Sci Coll Pharm State Key Lab Med Chem Biol Tianjin 300071 Peoples R China Tianjin Med Univ Canc Inst & Hosp Natl Clin Res Ctr Canc Tianjins Clin Res Ctr Canc Key Lab Canc Prevent & Therapy Tianjin 300060 Peoples R China

Cancer stem cells (CSCs) play a pivotal role in tumor initiation, proliferation, metastasis, drug resistance, and recurrence. Consequently, targeting CSCs has emerged as a promising avenue for cancer therapy. Recently, 3-phosphoglycerate dehydrogenase (PHGDH) has been identified as being intricately associated with the regulation of numerous cancer stem cells. Yet, reports detailing the functional regulators of PHGDH that can mitigate the stemness across cancer types are limited. In this study, the novel "molecular glue" LXH-3-71 was identified, and it robustly induced degradation of PHGDH, thereby modulating the stemness of colorectal cancer cells (CRCs) both in vitro and in vivo. Remarkably, LXH-3-71 was observed to form a dynamic chimera, between PHGDH and the DDB1-CRL E3 ligase. These insights not only elucidate the anti-CSCs mechanism of the lead compound but also suggest that degradation of PHGDH may be a more viable therapeutic strategy than the development of PHGDH inhibitors. Additionally, compound LXH-3-71 was leveraged as a novel ligand for the DDB1-CRL E3 ligase, facilitating the development of new PROTAC molecules targeting EGFR and CDK4 degradation. 2024 The Authors. Published by Elsevier B.V. on behalf of Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. This is an open access article under the CC BY-NC-ND license (http://***/licenses/by-nc-nd/4.0/).

关键词： Molecular glue Targeted protein degradation PHGDH Cancer stem cells PROTACs SERINE SYNTHESIS CANCER CELLS DIFFERENTIATION INHIBITOR REVEALS CLONING LIGASE

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双控制方式下带缆水下机器人轨迹跟踪与水动力响应分析

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船舶力学 2024年第7期28卷 967-980页

作者：陈东军吴家鸣拜永征扬州工业职业技术学院建筑工程学院江苏扬州225127 华南理工大学土木与交通学院广州510640 江苏智仝建设科技有限公司江苏扬州225009

本文首先根据已有的脐带缆运动控制方程,通过引入与工作船和水下机器人连接点处边界条件和速度耦合关系,与水下机器人运动方程一同构成完整的水动力数学模型;其次提出收放脐带缆的前馈-反馈控制方法和调节螺旋桨转速的增量式PID(Proport... 详细信息

本文首先根据已有的脐带缆运动控制方程,通过引入与工作船和水下机器人连接点处边界条件和速度耦合关系,与水下机器人运动方程一同构成完整的水动力数学模型;其次提出收放脐带缆的前馈-反馈控制方法和调节螺旋桨转速的增量式PID(Proportion Integration Differentiation)算法,建立完整的带缆水下机器人系统水动力与控制模型;最后对所提出的模型进行数值模拟实验验证与运动控制操纵下的水动力分析。数值计算结果表明:本文所提出的带缆水下机器人系统水动力与控制模型是有效可靠的,垂向运动控制过程中,水下机器人纵摇角、横摇角和沉深的模拟值与实验值最大误差分别为2°、1°和-50 mm;对于PID算法,调节螺旋桨转速控制水下机器人对给定位置信息的跟踪响应效果,模拟值与实验值相差均不大;轨迹跟踪模拟计算沿水平(X轴)方向和竖直(z.)方向最大相对误差分别为10%和15%;水下机器人竖向(沿z.)运动主要由调节脐带缆长度的前馈-反馈策略决定,沿水平(X轴)方向的运动主要由调节螺旋桨转速的PID算法控制;机器人水动力载荷受其周围流场变化的影响,而流场的变化主要由机器人运动速度的改变和螺旋桨转速变化二者共同决定。

关键词：运动方程水动力与控制模型带缆水下机器人前馈-反馈控制 PID算法

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固相外延生长法制备z.M-5@Silicalite-1分子筛及其CO_(2)加氢耦合甲苯烷基化反应的影响

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燃料化学学报（中英文） 2024年第8期52卷 1115-1130页

作者：贾艺敏牛鹏宇贾丽涛林明桂郭荷芹肖勇侯博李德宝中国科学院山西煤炭化学研究所煤炭高效低碳利用全国重点实验室山西太原030001 中国科学院大学北京100049 中国科学院洁净能源创新研究院辽宁大连116023

本研究采用固相法在z.M-5表面外延生长Silicalite-1,制备出z.M-5@Silicalite-1分子筛。同时制备高活性氧化物z.z.Ox,并与z.M-5@Silicalite-1物理混合组成z.z.Ox/z.M-5@Silicalite-1双功能催化剂,研究了CO_(2)加氢耦合甲苯烷基化催化性能。相比于z.z.Ox/z.M-5催化剂,分子筛改性后的双功能催化剂提高了对二甲苯(PX)选择性。研究了晶化条件(硅源、晶化过程、晶化次数)对z.M-5外延生长Silicalite-1的影响,以及Silicalite-1钝化层厚度对CO_(2)加氢耦合甲苯烷基化反应性能的影响。在400℃、3 MPa反应条件下,z.O/1:3.5z.-Na-SiO_(2)催化剂的甲苯转化率为12.0%,二甲苯选择性为77.4%,在二甲苯中对二甲苯选择性为73.4%。通过SEM、XRD、N2吸附-脱附、XPS、NH3-TPD、Py-FTIR等表征,研究了分子筛的结构和酸性质。结果表明,通过固相外延生长,延长z.M-5的孔道,增加间二甲苯(MX)、邻二甲苯(OX)的扩散阻力,同时钝化外表面的酸性,可以有效提高对二甲苯(PX)的选择性。固相外延生长法改性z.M-5分子筛,摒弃了以往堵塞孔以缩小孔口改性分子筛的缺点,在保证催化剂活性的同时提高了产物选择性。

关键词： MFI型分子筛外延生长对二甲苯固相法 CO_(2)加氢耦合甲苯烷基化

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石墨烯/GaN异质结构界面热输运性质的分子动力学研究

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物理学报 2024年第13期73卷 327-335页

作者：刘东静周福胡志亮黄家强桂林电子科技大学机电工程学院广西制造系统与先进制造技术重点实验室桂林541004

异质结构界面热输运的性能决定微纳米器件工作的可靠性.本文采用分子动力学方法,研究石墨烯/GaN三明治结构异质界面的热输运特性,分析温度、缺陷以及尺寸对异质结构界面热导的影响.研究发现,随着温度的升高,界面热导增大;当温度为1100 K... 详细信息

异质结构界面热输运的性能决定微纳米器件工作的可靠性.本文采用分子动力学方法,研究石墨烯/GaN三明治结构异质界面的热输运特性,分析温度、缺陷以及尺寸对异质结构界面热导的影响.研究发现,随着温度的升高,界面热导增大;当温度为1100 K时,3层石墨烯异质结构的界面热导增大61%,分析认为这主要是温度升高导致晶格振动增加,进而激发更多的平面外声子.GaN中的少量空位缺陷导致界面热导增大,当缺陷率为20%时,界面热导达到最大值0.0357 GW/(m^(2)·K),分析认为缺陷会增加额外的热输运通道;随后界面热导开始降低,分析认为缺陷率增大导致界面耦合强度降低.随着GaN层数从8层增加到24层,界面热导下降,计算声子模态发现参与界面热输运声子数量减少;而石墨烯从2层到6层时,界面热导先上升后下降,分析认为这是与声子匹配和耦合强度的初期提高和后期声子散射及局部化增加有关.该研究结果为调控微电子器件界面热输运提供理论依据.

关键词：异质结构界面热导空位缺陷尺寸效应

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RECIPROCATING EXTRUSION OF IN SITU Mg_2Si REINFORCED Mg-Al BASED COMPOSITE

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Acta Metallurgica Sinica(English Letters) 2008年第3期21卷 169-177页

作者： z.M. z.ang C.J. Xu X.F. Guo S.z. jia School of Materials Science ＆ Engineering Xi＇an University of Technology Xi＇an 710048 China

M92Si reinforced Mg-Al based composite with high amount o/silicon was prepared by permanent mould casting, and then extruded by reciprocating extrusion （RE） after the composite was processed by homogeniz.tion heat treatment. The effect of RE processing on the morphology and siz. of M92Si and the mechanical properties of the com- posite were investigated, to develop new ways to refine the M928i phase and improve its shape. The result showed that RE was very useful in refining the M92Si phase. The more the RE processing passes, the better the refining effect would be. Moreover, the uniform distribution of M928i phases would be more in the composite. After the composite was processed by RE for 12 passes, most M92Si phases were equiaxed, with granular diameter below 20 μm, and distributed uniformly in the matrix of the composite. The mechanical properties of the composite could be increased prominently by RE processing, and were much higher than that in the as-cast state. As the temperature rises, the tensile strength is reduced. For the composite RE processed for 12 passes, the tensile strength, yield strength, and elongation are 325.9 MPa, 211.4 MPa, and 3.3% at room temperature, whereas, 288.2 MPa, ,207.7 MPa, and 7.8%, respectively, at 150℃. In comparison with the properties at room temperature, the tensile strength and yield strength are high and only decrease by 11.6% and 1.8% at 150℃. The M928i reinforced Mg-Al based composite possesses good heat resistance at 150℃. The excellent resistance to effect of heat is attributed to the high melting tempera- ture and good thermal stability of fine Mg2Si phases, which are distributed uniformly in the composite, and effectively hinder the grain boundary gliding and dislocation movement.

关键词： Reciprocating extrusion Magnesium alloy In situ composite Elevated temperature property

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文体旅融合视角下的天府绿道发展路径研究

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四川体育科学 2024年第1期43卷 115-120页

作者：杜佳西南民族大学体育学院四川成都610041.

当前成都市体育产业规模达到近千亿元,产业贡献度日益凸显,已经成为促进经济增长动能转换、推动经济社会转型的重要引擎,绿道体育经济在成都全面建设践行新发展理念的公园城市示范区和推动高质量发展、创造高品质生活中具有重要作用。... 详细信息

当前成都市体育产业规模达到近千亿元,产业贡献度日益凸显,已经成为促进经济增长动能转换、推动经济社会转型的重要引擎,绿道体育经济在成都全面建设践行新发展理念的公园城市示范区和推动高质量发展、创造高品质生活中具有重要作用。本文对现有天府绿道体育相关产业的运营方式、特色进行调研,总结归纳文化旅游相关项目在绿道体育产业中起到的积极作用,以及融合发展对成都经济可持续发展的正面影响。对比国内外优秀绿道体育发展案例,找出适合天府绿道体育发展的并且具有成都地域文化特色的项目,为成都推动以绿色新优势的可持续发展,建造高品质公园城市场景提供发展策略。

关键词：文体旅融合天府绿道产业融合发展路径

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Evidence for particle acceleration approaching PeV energies in the W51 complex

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Science Bulletin 2024年第18期69卷 2833-2841页

作者： Cao, z.en Aharonian, F. Axikegu Bai, Y.X. Bao, Y.W. Bastieri, D. Bi, X.J. Bi, Y.J. Bian, W. Bukevich, A.V. Cao, Q. Cao, W.Y. Cao, z.e Chang, J. Chang, J.F. Chen, A.M. Chen, E.S. Chen, H.X. Chen, Liang Chen, Lin Chen, Long Chen, M.J. Chen, M.L. Chen, Q.H. Chen, S. Chen, S.H. Chen, S.z. Chen, T.L. Chen, Y. Cheng, N. Cheng, Y.D. Cui, M.Y. Cui, S.W. Cui, X.H. Cui, Y.D. Dai, B.z. Dai, H.L. Dai, z.G. Danz.ngluobu Dong, X.Q. Duan, K.K. Fan, J.H. Fan, Y.z. Fang, J. Fang, J.H. Fang, K. Feng, C.F. Feng, H. Feng, L. Feng, S.H. Feng, X.T. Feng, Y. Feng, Y.L. Gabici, S. Gao, B. Gao, C.D. Gao, Q. Gao, W. Gao, W.K. Ge, M.M. Geng, L.S. Giacinti, G. Gong, G.H. Gou, Q.B. Gu, M.H. Guo, F.L. Guo, X.L. Guo, Y.Q. Guo, Y.Y. Han, Y.A. Hasan, M. He, H.H. He, H.N. He, J.Y. He, Y. Hor, Y.K. Hou, B.W. Hou, C. Hou, X. Hu, H.B. Hu, Q. Hu, S.C. Huang, D.H. Huang, T.Q. Huang, W.J. Huang, X.T. Huang, X.Y. Huang, Y. Ji, X.L. jia, H.Y. jia, K. jiang, K. jiang, X.W. jiang, z.J. Jin, M. Kang, M.M. Karpikov, I. Kuleshov, D. Kurinov, K. Li, B.B. Li, C.M. Li, Cheng Li, Cong Li, D. Li, F. Li, H.B. Li, H.C. Li, jian Li, Jie Li, K. Li, S.D. Li, W.L. Li, X.R. Li, Xin Li, Y.z. Li, z.e Li, z.uo Liang, E.W. Liang, Y.F. Lin, S.J. Liu, B. Liu, C. Liu, D. Liu, D.B. Liu, H. Liu, H.D. Liu, J. Liu, J.L. Liu, M.Y. Liu, R.Y. Liu, S.M. Liu, W. Liu, Y. Liu, Y.N. Luo, Q. Luo, Y. Lv, H.K. Ma, B.Q. Ma, L.L. Ma, X.H. Mao, J.R. Min, z. Mitthumsiri, W. Mu, H.J. Nan, Y.C. Neronov, A. Ou, L.J. Pattarakijwanich, P. Pei, z.Y. Qi, J.C. Qi, M.Y. Qiao, B.Q. Qin, J.J. Raz., A. Ruffolo, D. Sáiz. A. Saeed, M. Semikoz. D. Shao, L. Shchegolev, O. Sheng, X.D. Shu, F.W. Song, H.C. Stenkin, Yu.V. Stepanov, V. Su, Y. Sun, D.X. Sun, Q.N. Sun, X.N. Sun, z.B. Takata, J. Tam, P.H.T. Key Laboratory of Particle Astrophysics & Experimental Physics Division & Computing Center Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China University of Chinese Academy of Sciences Beijing 100049 China TIANFU Cosmic Ray Research Center Chengdu China Dublin Institute for Advanced Studies 31 Fitzwilliam Place 2 Dublin Ireland Max-Planck-Institut for Nuclear Physics P.O. Box 103980 Heidelberg 69029 Germany School of Physical Science and Technology & School of Information Science and Technology Southwest Jiaotong University Chengdu 610031 China School of Astronomy and Space Science Nanjing University Nanjing 210023 China Center for Astrophysics Guangzhou University Guangzhou 510006 China Tsung-Dao Lee Institute & School of Physics and Astronomy Shanghai Jiao Tong University Shanghai 200240 China Institute for Nuclear Research of Russian Academy of Sciences Moscow 117312 Russia Hebei Normal University Shijiazhuang 050024 China University of Science and Technology of China Hefei 230026 China State Key Laboratory of Particle Detection and Electronics China Key Laboratory of Dark Matter and Space Astronomy & Key Laboratory of Radio Astronomy Purple Mountain Observatory Chinese Academy of Sciences Nanjing 210023 China Research Center for Astronomical Computing Zhejiang Laboratory Hangzhou 311121 China Key Laboratory for Research in Galaxies and Cosmology Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China School of Physics and Astronomy Yunnan University Kunming 650091 China Key Laboratory of Cosmic Rays (Tibet University) Ministry of Education Lhasa 850000 China National Astronomical Observatories Chinese Academy of Sciences Beijing 100101 China School of Physics and Astronomy (z.uhai) & School of Physics (Guangz.ou) & Sino-French Institute of Nuclear Engineering and Technology (z.uhai) Sun Yat-sen University Zhuhai 519000 & Guangzhou 510275 Guangdong China Institute of Frontier and

The γ-ray emission from the W51 complex is widely acknowledged to be attributed to the interaction between the cosmic rays (CRs) accelerated by the shock of supernova remnant (SNR) W51C and the dense molecular clouds in the adjacent star-forming region, W51B. However, the maximum acceleration capability of W51C for CRs remains elusive. Based on observations conducted with the Large High Altitude Air Shower Observatory (LHAASO), we report a significant detection of γ rays emanating from the W51 complex, with energies from 2 to 200 TeV. The LHAASO measurements, for the first time, extend the γ-ray emission from the W51 complex beyond 100 TeV and reveal a significant spectrum bending at tens of TeV. By combining the "π0-decay bump" featured data from Fermi-LAT, the broadband γ-ray spectrum of the W51 region can be well-characteriz.d by a simple pp-collision model. The observed spectral bending feature suggests an exponential cutoff at ∼400 TeV or a power-law break at ∼200 TeV in the CR proton spectrum, most likely providing the first evidence of SNRs serving as CR accelerators approaching the PeV regime. Additionally, two young star clusters within W51B could also be theoretically viable to produce the most energetic γ rays observed by LHAASO. Our findings strongly support the presence of extreme CR accelerators within the W51 complex and provide new insights into the origin of Galactic CRs. © 2024 Science China Press

关键词： Cosmic rays

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Valleytronic topological filters in silicene-like inner-edge systems

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Chinese Physics B 2024年第1期33卷 66-74页

作者：谢航吕小龙杨加恩 College of Physics Chongqing UniversityChongqing 401331China Chongqing Key Laboratory for Strongly-Coupled Physics Chongqing UniversityChongqing 401331China College of Science Guangxi University of Science and TechnologyLiuzhou 545006China School of Electronics and IoT Chongqing College of Electronic EngineeringChongqing 401331China

Inner edge state with spin and valley degrees of freedom is a promising candidate for designing a dissipationless device due to the topological protection. The central challenge for the application of the inner edge state is to generate and modulate the polariz.d currents. In this work, we discover a new mechanism to generate fully valley-and spin–valley-polariz.d current caused by the Bloch wavevector mismatch(BWM). Based on this mechanism, we design some serial-typed inner-edge filters. By using once of the BWM, the coincident states could be divided into transmitted and reflected modes, which can serve as a valley or spin–valley filter. In particular, while with twice of the BWM, the incident current is absolutely reflected to support an off state with a specified valley and spin, which is different from the gap *** findings give rise to a new platform for designing valleytronics and spin-valleytronics.

关键词： topological insulator inner-edge state valley polariz.tion spin polariz.tion quantum transport

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