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Synaptotagmins family affect glucose transport in retinal pigment epithelial cells through their ubiquitination-mediated degradation and glucose transporter-1 regulation

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World journal of Diabetes 2024年第5期15卷 958-976页

作者： hong Xu Li-Bo Zhang Yi-Yi Luo Ling Wang Ye-Pin Zhang Pei-Qi Chen Xue-Ying Ba jian Han Heng Luo Department of Ophthalmology The People’s Hospital of Chuxiong Yi Autonomous Prefecture&The Fourth Affiliated Hospital of Dali UniversityChuxiong Yi Autonomous Prefecture 675000Yunnan ProvinceChina Precision Medicine Center of Chuxiong Yi Autonomous Prefecture The People’s Hospital of Chuxiong Yi Autonomous Prefecture&The Fourth Affiliated Hospital of Dali UniversityChuxiong Yi Autonomous Prefecture 675000Yunnan ProvinceChina Department of Endocrinology The People’s Hospital of Chuxiong Yi Autonomous Prefecture&The Fourth Affiliated Hospital of Dali UniversityChuxiong Yi Autonomous Prefecture 675000Yunnan ProvinceChina Department of Pathology The People’s Hospital of Chuxiong Yi Autonomous Prefecture&The Fourth Affiliated Hospital of Dali UniversityChuxiong Yi Autonomous Prefecture 675000Yunnan ProvinceChina

BACKGROUND Synaptotagmins(SYTs)are a family of 17 membrane transporters that function as calcium ion sensors during the release of Ca2+-dependent neurotransmitters and ***,few studies have reported whether members of the SYT family play a role in glucose uptake in diabetic retinopathy(DR)through Ca2+/glucose transporter-1(GLUT1)and the possible regulatory mechanism of *** To elucidate the role of the SYT family in the regulation of glucose transport in retinal pigment epithelial cells and explore its potential as a therapeutic target for the clinical management of *** DR was induced by streptozotocin in C57BL/6j mice and by high glucose medium in human retinal pigment epithelial cells(ARPE-19).Bioinformatics analysis,reverse transcriptase-polymerase chain reaction,Western blot,flow cytometry,ELISA,HE staining,and TUNEL staining were used for *** Six differentially expressed proteins(SYT2,SYT3,SYT4,SYT7,SYT11,and SYT13)were found between the DR and control groups,and SYT4 was highly *** induces SYT4 overexpression,manipulates Ca2+influx to induce GLUT1 fusion with the plasma membrane,promotes abnormal expression of the glucose transporter GLUT1 and excessive glucose uptake,induces ARPE-19 cell apoptosis,and promotes DR *** deficiency inhibits the proteasomal degradation of SYT4 in DR,resulting in SYT4 accumulation and enhanced GLUT1 fusion with the plasma membrane,and these effects were blocked by oe-Parkin ***,dysregulation of the myelin transcription factor 1(Myt1)-induced transcription of SYT4 in DR further activated the SYT4-mediated stimulus-secretion coupling process,and this process was inhibited in the oe-MYT1-treated *** Our study reveals the key role of SYT4 in regulating glucose transport in retinal pigment epithelial cells during the pathogenesis of DR and the underlying mechanism and suggests potential therapeutic targets for clinical DR.

关键词： Diabetic retinopathy Glucose transporter-1 Synaptotagmin 4 Parkin Myelin transcription factor 1

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高压大容量柔性直流换流阀关键设计和发展趋势

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电源学报 2024年第3期22卷 1-14页

作者：饶宏周月宾陈煜坤杨柳赵彪易荣盛俊毅直流输电技术全国重点实验室(南方电网科学研究院有限责任公司) 广州510663 清华大学电机系北京100084 荣信汇科电气股份有限公司鞍山114051 特变电工西安柔性输配电有限公司西安710061

柔性直流输电技术具有控制灵活性高、无换相失败问题和动态无功支撑能力强等突出优点,已广泛应用于点对点输电、背靠背联网和构建直流电网等场景。柔性直流换流阀通过功率器件的频繁开断来实现交、直流的电能转换,是柔性直流输电工程的... 详细信息

柔性直流输电技术具有控制灵活性高、无换相失败问题和动态无功支撑能力强等突出优点,已广泛应用于点对点输电、背靠背联网和构建直流电网等场景。柔性直流换流阀通过功率器件的频繁开断来实现交、直流的电能转换,是柔性直流输电工程的核心设备。首先,结合工程实践经验,系统梳理并提出了不同应用场景下柔性直流换流阀的关键设计要求,对比分析了柔性直流输电工程中常用的功率器件及柔性直流换流阀拓扑,展望了柔性直流换流阀的发展趋势。此外,针对2种典型的未来应用场景,对比了不同的技术方案,可为柔性直流输电技术应用于高压大容量远距离输电场景提供有益借鉴。

关键词：电压源型换流器高压直流输电模块化多电平换流器电力电子器件新能源

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伯仲胺基团修饰Fe_(3)O_(4)磁性材料的制备和性能表征

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复合材料学报 2024年第4期41卷 1977-1986页

作者：周书威傅红杨方福州海关技术中心福州350001 福州大学生物科学与工程学院福州350108

食品中的有机酸等内源性物质会干扰其中痕量残留物的检测,为消除此影响,以N-β-(氨乙基)-γ-氨丙基三甲氧基硅烷(KH792)为氨基化试剂,直接修饰酸化后的Fe_(3)O_(4)得到磁性吸附材料。经响应面试验优化得到KH792对酸化Fe_(3)O_(4)的最佳... 详细信息

食品中的有机酸等内源性物质会干扰其中痕量残留物的检测,为消除此影响,以N-β-(氨乙基)-γ-氨丙基三甲氧基硅烷(KH792)为氨基化试剂,直接修饰酸化后的Fe_(3)O_(4)得到磁性吸附材料。经响应面试验优化得到KH792对酸化Fe_(3)O_(4)的最佳修饰条件为温度82.5℃、pH=4.9,KH792添加量1.8 mL,在最佳条件下,KH792直接修饰的酸化Fe_(3)O_(4)对没食子酸吸附量为22.8 mg/g,比市售Fe_(3)O_(4)提高了188%,且具备快速固液分离的能力、良好的稳定性及分散性。通过BET、Zeta电位、FTIR和XPS对直接修饰的产物性能进行了表征,结果表明,KH792与Fe_(3)O_(4)表面羟基通过Fe−O−Si键结合,酸化提高了Fe_(3)O_(4)表面羟基含量,因此也增强了KH792对Fe_(3)O_(4)的修饰效果,本文制备的磁性产物可用于净化复杂样品基质中的有机酸。

关键词： N-β-(氨乙基)-γ-氨丙基三甲氧基硅烷酸化Fe_(3)O_(4) 直接修饰法磁性吸附材料修饰机制

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香鱼LECT2双抗体夹心ELISA法的建立及初步应用

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农业生物技术学报 2024年第7期32卷 1703-1714页

作者：许立琼史雨红陈炯宁波大学农产品质量安全危害因子与风险防控国家重点实验室宁波大学海洋学院生物化学与分子生物学实验室宁波大学海洋学院水产生物技术教育部重点实验室

白细胞衍生趋化因子(leucocyte cell-derived chemotaxin 2, LECT2)的表达量对鱼类健康状态具有指示作用。本研究利用毕赤酵母(Pichia pastoris)表达香鱼(Plecoglossus altivelis)重组PaLECT2蛋白(recombinant PaLECT2, rPaLECT2)，分别免疫小鼠(Mus musculus)和新西兰白兔(Oryctolagus cuniculus)制备抗血清，再用Protein G亲和层析柱纯化获得抗体。以小鼠抗PaLECT2抗体作为捕获抗体以及辣根过氧化酶(horseradish peroxidase, HRP)标记的兔抗PaLECT2抗体作为检测抗体，通过条件优化建立双抗体夹心酶联免疫吸附法(double-antibody sandwich enzyme-linked immunosorbent assay, DAS-ELISA)。最后应用该方法检测嗜水气单胞菌(Aeromonas hydrophila)感染香鱼的模型和抗生素治疗的预后模型中血清PaLECT2蛋白含量。研究结果显示，小鼠和HRP-兔抗PaLECT2抗体效价分别为1∶12 800和1∶25 600。当捕获抗体浓度为8μg/mL而检测抗体稀释比例为1∶1 600时，为最佳捕获抗体包被浓度和检测抗体工作浓度。所建DAS-ELISA法的最低检测限为11.22 ng/mL，具有良好的特异性和重复性。应用本方法测得感染组中PaLECT2的含量为(412±31.5) ng/mL，极显著高于健康组(54±7.2) ng/mL (P<0.001)，而治疗组中PaLECT2的含量为(225±56.1) ng/mL，极显著低于感染组(P<0.01)。综上，本研究为PaLECT2蛋白表达水平的定量检测提供了一种更加快捷、标准化的方法，有望应用于香鱼健康状况诊断和监测。

关键词：白细胞衍生趋化因子(LECT2) 双抗体夹心ELISA法(DAS-ELISA) 香鱼初步应用

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Association between glucose levels of children with type 1 diabetes and parental economic status in mobile health application

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World journal of Diabetes 2024年第7期15卷 1477-1488页

作者： Wen-Hao Zhang Chao-Fan Wang Hao Wang jie Tang hong-Qiang Zhang jiang-Yu Zhu Xue-Ying Zheng Si-Hui Luo Yu Ding Department of Endocrinology The First Affiliated Hospital of University of Science and Technology of ChinaDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefei 230001Anhui ProvinceChina Department of Endocrinology and Metabolism The Third Affiliated Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of DiabetologyGuangzhou 510000Guangdong ProvinceChina Graduate School Bengbu Medical CollegeBengbu 233000Anhui ProvinceChina

BACKGROUND The glycemic control of children with type 1 diabetes(T1D)may be influenced by the economic status of their *** To investigate the association between parental economic status and blood glucose levels of children with T1D using a mobile health *** Data from children with T1D in China's largest T1D online community,Tang-TangQuan■.Blood glucose levels were uploaded every three months and parental economic status was evaluated based on annual household *** were divided into three groups:Low-income(100000 yuan)(1 Yuan=0.145 United States Dollar approximately).Blood glucose levels were compared among the groups and associations were explored using Spearman’s correlation analysis and multivariable logistic *** From September 2015 to August 2022,1406 eligible children with T1D were included(779 female,55.4%).Median age was 8.1 years(Q1-Q3:4.6-11.6)and duration of T1D was 0.06 years(0.02-0.44).Participants were divided into three groups:Low-income(n=320),middle-income(n=724),and high-income(n=362).Baseline hemoglobin A1c(HbA1c)levels were comparable among the three groups(P=0.072).However,at month 36,the low-income group had the highest HbA1c levels(P=0.036).Within three years after registration,glucose levels increased significantly in the low-income group but not in the middle-income and high-income *** economic status was negatively correlated with pre-dinner glucose(r=-0.272,P=0.012).After adjustment for confounders,parental economic status remained a significant factor related to pre-dinner glucose levels(odds ratio=13.02,95%CI:1.99 to 126.05,P=0.002).CONCLUSION The blood glucose levels of children with T1D were negatively associated with parental economic *** is suggested that parental economic status should be taken into consideration in the management of T1D for children.

关键词： Type 1 diabetes Children and adolescents Glycemic control Economic status Mobile health application

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螺旋CT对急性重症胰腺炎诊断效能及对出血分型和并发症检出情况分析

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当代医学 2024年第4期30卷 151-154页

作者：陈小华饶驭徐小艳洪欣抚州市第一人民医院影像科江西抚州344000 抚州市第一人民医院超声科江西抚州344000

目的分析螺旋CT对急性重症胰腺炎诊断效能及出血分型、并发症检出情况。方法回顾性分析2019年1月至2022年1月抚州市第一人民医院收治的40例急性重症胰腺炎患者及本院同期40名健康体检者螺旋CT、腹部超声检查的影像学资料,比较螺旋CT与... 详细信息

目的分析螺旋CT对急性重症胰腺炎诊断效能及出血分型、并发症检出情况。方法回顾性分析2019年1月至2022年1月抚州市第一人民医院收治的40例急性重症胰腺炎患者及本院同期40名健康体检者螺旋CT、腹部超声检查的影像学资料,比较螺旋CT与腹部超声检查对急性重症胰腺炎的诊断效能及对急性重症胰腺炎患者胰腺出血分型及并发症的检出率。结果螺旋CT对急性重症胰腺炎诊断准确度、灵敏度、特异度分别为96.25%、95.00%、97.50%,均高于腹部超声检查的81.25%、77.50%、80.00%(P<0.05)。螺旋CT对Ⅰ型、Ⅱ型胰腺出血及血管异常、胰腺假性囊肿的检出率均高于腹部超声检查(P<0.05);螺旋CT与腹部超声对Ⅲ型胰腺出血、胃底静脉曲张、胸腔积液的检出率比较差异无统计学意义。结论与腹部超声相比,螺旋CT对急性重症胰腺炎的诊断价值较高,且对胰腺出血分型评估及并发症的检出率较高。

关键词：急性重症胰腺炎螺旋CT 并发症

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成年人非心胸手术中通气管理的临床实践:一项全国问卷调查研究

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国际麻醉学与复苏杂志 2024年第2期45卷 168-173页

作者：饶倩倩玉红王思洋余海四川大学华西医院麻醉科成都610041 复旦大学中山医院麻醉科上海200032

目的调查中国麻醉医师在成年人非心胸手术中通气管理的临床实践现状。方法2021年8月至2021年9月,向中国81家大型三甲综合医院麻醉科发出电子调查问卷进行全国调查性研究,分析小潮气量(V_(T))通气、呼气末正压通气(PEEP)及肺复张手法(ARM... 详细信息

目的调查中国麻醉医师在成年人非心胸手术中通气管理的临床实践现状。方法2021年8月至2021年9月,向中国81家大型三甲综合医院麻醉科发出电子调查问卷进行全国调查性研究,分析小潮气量(V_(T))通气、呼气末正压通气(PEEP)及肺复张手法(ARM)的应用情况和“肺保护性通气(LPV)策略”的理论认识情况。对调查结果进行描述性统计分析。结果全国57家调查中心参与调查,调查中心回复率为70%,回收有效问卷1089份。92.7%的麻醉医师术中常规使用小VT通气。77.1%的麻醉医师术中常规设置PEEP,1~5 cmH_(2)O(1 cmH_(2)O=0.098 kPa)是最常采用的PEEP水平。84.6%的麻醉医师术中常规实施ARM。绝大多数麻醉医师(97.9%)认为“LPV策略”是指联合使用“小V_(T)、PEEP及ARM”。64.8%的麻醉医师术中常规联合使用小V_(T)、PEEP及ARM。结论中国大型三甲综合医院的麻醉医师在成年人非心胸手术中通气管理中的临床实践现状存在异质性,LPV策略的临床实践与理论认识存在一定差距。

关键词：问卷调查非心胸手术机械通气肺保护性通气策略

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外伤性颈动脉血栓形成致血栓栓塞性脑梗死残疾等级评定1例

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法医学杂志 2024年第1期40卷 92-94页

作者：陈红羽刘凯洪仕君信阳市中心医院医学影像科河南信阳464000 河南圣德司法鉴定中心河南信阳464000 昆明医科大学法医学院云南昆明650500

1案例1.1简要案情王某,男,16岁,某年3月12日晚骑电瓶车行驶过程中被电杆拉线绊住颈部摔倒,当即感头痛。急诊入院后行头颅CT检查未显示明显异常,遂对颈部右侧创口行清创缝合术。3月14日20:00许,王某在洗手时晕倒,后感头晕、头疼,左侧肢... 详细信息

1案例1.1简要案情王某,男,16岁,某年3月12日晚骑电瓶车行驶过程中被电杆拉线绊住颈部摔倒,当即感头痛。急诊入院后行头颅CT检查未显示明显异常,遂对颈部右侧创口行清创缝合术。3月14日20:00许,王某在洗手时晕倒,后感头晕、头疼,左侧肢体无力,被送入院治疗。1.2病史摘要某年3月14日21:00许王某至某医院就诊,血压16/11 kPa(120/82 mmHg),既往体健,无高血压、动脉硬化史。专科检查:嗜睡,呼唤睁眼;两侧颈部对称,颈部右前有一挫裂创(已缝合),局部红肿、无渗出;左侧肢体肌力0级,肌张力高。

关键词：法医学创伤和损伤颈动脉血栓血栓栓塞脑梗死残疾评定

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Comparative Analysis of Energy Characteristics of Two Southwest Vortices in Sichuan Under Similar Circulation Backgrounds

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journal of Tropical Meteorology 2024年第2期30卷 168-179页

作者：周春花张驹肖红茹 Sichuan Meteorological Disaster Prevention Technology Center Chengdu 610072 China Chengdu Meteorological Bureau Chengdu 610072 China Sichuan Meteorological Observatory Chengdu 610072 China

Based on ERA5 reanalysis data,the present study analyzed the thermal energy development mechanism and kinetic energy conversion characteristics of two extreme rainstorm processes in relation to the shallow southwest vortex in the warm-sector during a“rain-generated vortex”process and the deep southwest vortex in a“vortex-generated rain”*** findings were as follows:(1)During the extreme rainstorm on August 11,2020(hereinafter referred to as the“8·11”process),intense surface heating and a high-energy unstable environment were *** mesoscale convergence system triggered convection to produce heavy rainfall,and the release of latent condensation heat generated by the rainfall promoted the formation of a southwest *** significant increase(decrease)in atmospheric diabatic heating and kinetic energy preceded the increase(decrease)in *** contrast,the extreme rainstorm on August 16,2020(hereinafter referred to as the“8·16”process)involved the generation of southwest vortex in a low-energy and highhumidity *** dynamic uplift of the southwest vortex triggered rainfall,and the release of condensation latent heat from rainfall further strengthened the development of the southwest *** significant increase(decrease)in atmospheric diabatic heating and kinetic energy exhibited a delayed progression compared to the increase(decrease)in vorticity.(2)The heating effect around the southwest vortex region was non-uniform,and the heating intensity varied in different *** the“8·11”process,the heating effect was the strongest in the initial stage,but weakened during the vortex's *** the contrary,the heating effect was initially weak in the“8·16”process,and intensified during the development stage.(3)The available potential energy of the“8·11”process significantly increased in kinetic energy converted from rotational and divergent winds through baroclinic action,and the divergent wind energy continued to convert into rotatio

关键词： southwest vortex similar circulation background diabatic heating kinetic energy spatial non-uniform heating effect

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The potential mechanism of Isodon suzhouensis against COVID-19 via EGFR/TLR4 pathways

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Food Science and Human Wellness 2024年

作者： hong Duan Wei Wang Shu Li Han Li Ghulam jilany Khan Fawang Liu Kefeng Zhai Lili Li Zhaojun Wei General Clinical Research Center Anhui Wanbei Coal-Electricity Group General Hospital Department of Pharmacology and Therapeutics Faculty of Pharmacy University of Central Punjab College of Biological and Food Engineering Anhui Polytechnic University School of Biological and Food Engineering Engineering Research Center for Development and High Value Utilization of Genuine Medicinal Materials in North Anhui Province Suzhou University School of Food and Biological Engineering Hefei University of Technology

Corona Virus Disease 2019 (COVID-19) has brought the new challenges to scientific research. Isodon suzhouensis has good anti-inflammatory and antioxidant stress effects， which is considered as a potential treatment for COVID-19. The possibility for the treatment of COVID-19 with suzhouensis and its potential mechanism of action were explored by employing molecular docking and network pharmacology. Network pharmacology and molecular docking were used to screen drug targets， and Lipopolysaccharide (LPS) induced RAW264.7 and NR8383 cells inflammation model was used for experimental verification. Collectively a total of 209 possible linkages against 18 chemical components from suzhouensis and 1194 COVID-19 related targets were selected. Among these， 164 common targets were obtained from the intersection of suzhouensis and COVID-19. GO and KEGG enriched 582 function targets and 87 target proteins pathways， respectively. The results from molecular docking studies revealed that rutin， vitexin， isoquercitrin and quercetin had significant binding ability with SARS-CoV-23CLpro and ACE2. In vitro studies showed that Isodon suzhouensis extract (ISE) may inhibit the activation of PI3K/Akt pathway and the expression level of downstream pro-inflammatory factors by inhibiting the activation of EGFR in RAW264.7 cells induced by LPS. In addition， ISE was able to inhibit the activation of TLR4/NF-κB signaling pathway in NR8383 cells exposed to LPS. Overall， the network pharmacology and in vitro studies conclude that active components from suzhouensis have strong therapeutic potential against COVID-19 through multi-target， multi-pathway dimensions and can be a promising candidate against COVID-19.

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