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Intravitreal injection practice patterns among Chinese ophthalmologists

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International journal of Ophthalmology(English edition) 2024年第9期17卷 1717-1722页

作者： Li Su Yin-Chen shen Hong Li Bo Mou Kun Liu Xun Xu Department of Ophthalmology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200080China National Clinical Research Center for Eye Diseases Shanghai 200080China Shanghai Key Laboratory of Ocular Fundus Diseases Shanghai 200080China Shanghai MicroCall Information Technology Co. Ltd.Shanghai 200436China

AIM:To describe the practice patterns of intravitreal injections(IVIs)among ophthalmologists in ***:This was a cross-sectional online *** who had performed accumulated more than 100 injections were contacted by the Brightness Center,a hospital-based national network,to complete an anonymous,24-question,internet-based *** were surveyed on practices in injection techniques,pre-,and post-injections ***:A total of 333 ophthalmologists from 28 provinces/municipalities/autonomous regions responded to the survey(50.68%response rate).The 91.29%of the respondents evaluated systemic risk factors by medical history,electrocardiogram(ECG)and blood *** the respondents used pre-injection prophylactic *** checked intraocular pressure(IOP,99.1%)and blood pressure(96.1%)before injections.A majority of the respondents performed injections in the operating room(98.8%),wore masks(99.7%),gloves(99.4%)and sterile surgical clothing(96.1%),performed topical anesthetics(97.9%),and applied povidone-iodine(95.8%)*** 61.26%of the respondents dilated *** half of the respondents(51.05%)performed bilateral injections in the same *** temporal quadrant(40.54%)was the most frequent site of *** three quarters used 30-gauge *** respondents(97.9%)measured the site of injection from *** than half(53.45%)performed conjunctiva displacement prior to *** 32.43%of the respondents checked IOP post-injection and 87.99%physicians checked hand motion(HM)or counting fingers(CF)after injection,while 36.94%observed optic nerve *** participants used topical antibiotics *** physicians(91.89%)reviewed patients on the following ***:This study provides a description of the real-world practice patterns in IVIs in China and offers critical information regarding education and training of ophthalmologists and amendment of local society guideli

关键词： intravitreal injections practice pattern topical antibiotics Chinese ophthalmologists

Non-crossing Quantile Regression Neural Network as a Calibration Tool for Ensemble Weather Forecasts

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Advances in Atmospheric Sciences 2024年第7期41卷 1417-1437页

作者： Mengmeng SONG Dazhi YANG Sebastian LERCH Xiang'ao XIA Gokhan Mert YAGLI jamie M.BRIGHT Yanbo shen Bai LIU Xingli LIU Martin janos MAYER School of Electrical Engineering and Automation Harbin Institute of TechnologyHarbin150001China Heidelberg Institute for Theoretical Studies Schloss-Wolfsbrunnenweg 3569118HeidelbergGermany Chair of Statistical Methods and Econometrics Karlsruhe Institute of Technology(KIT)Bluecherstr.1776185KarlsruheGermany Key Laboratory of Middle Atmosphere and Global Environment Observation Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing100029China Solar Energy Research Institute of Singapore(SERIS) National University of Singapore(NUS)Singapore117574Singapore UK Power Networks LondonSE61NPUK China Meteorological Administration Beijing100081China Heilongjiang Meteorological Bureau Harbin150001China Department of Energy Engineering Faculty of Mechanical EngineeringBudapest University of Technology and EconomicsMűegyetem rkp.3H-1111BudapestHungary

Despite the maturity of ensemble numerical weather prediction(NWP),the resulting forecasts are still,more often than not,*** such,forecast calibration tools have become *** those tools,quantile regression(QR)is highly competitive in terms of both flexibility and predictive ***,a long-standing problem of QR is quantile crossing,which greatly limits the interpretability of QR-calibrated *** this point,this study proposes a non-crossing quantile regression neural network(NCQRNN),for calibrating ensemble NWP forecasts into a set of reliable quantile forecasts without *** overarching design principle of NCQRNN is to add on top of the conventional QRNN structure another hidden layer,which imposes a non-decreasing mapping between the combined output from nodes of the last hidden layer to the nodes of the output layer,through a triangular weight matrix with positive *** empirical part of the work considers a solar irradiance case study,in which four years of ensemble irradiance forecasts at seven locations,issued by the European Centre for Medium-Range Weather Forecasts,are calibrated via NCQRNN,as well as via an eclectic mix of benchmarking models,ranging from the naïve climatology to the state-of-the-art deep-learning and other non-crossing *** and stringent forecast verification suggests that the forecasts post-processed via NCQRNN attain the maximum sharpness subject to calibration,amongst all ***,the proposed conception to resolve quantile crossing is remarkably simple yet general,and thus has broad applicability as it can be integrated with many shallow-and deep-learning-based neural networks.

关键词： ensemble weather forecasting forecast calibration non-crossing quantile regression neural network CORP reliability diagram post-processing

The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution

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journal of Integrative Plant Biology 2024年第9期66卷 2058-2075页

作者： Yuan‐Bao Li Chengyu Liu Ningning shen Shuai Zhu Xianya Deng Zixuan Liu Li‐Bo Han Dingzhong Tang State Key Laboratory of Ecological Control of Fujian‐Taiwan Crop Pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350002China College of Agriculture Fujian Agriculture and Forestry UniversityFuzhou 350002China College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China School of Future Technology Fujian Agriculture and Forestry UniversityFuzhou 350002China

Magnaporthe oryzae(***)is a devastating hemibiotrophic *** biotrophic invasive hyphae(IH)are enclosed in the extrainvasive hyphal membrane produced by plant cells,thus generating a front line of the battlefield between the pathogen and the host *** plants,defense-related complexes such as proteins,callose-rich materials and vesicles,are directionally secreted to this interface to confer defense responses,but the underlying molecular mechanism is poorly *** this study,we found that a Myosin gene,Myosin A1(OsMYA1),contributed to rice *** OsMYA1 knockout mutant exhibited decreased resistance to *** ***1 localizes to the actin cytoskeleton and surrounds the IH of ***1 interacts with an exocyst subunit,OsExo70H1,and regulates its accumulation at the plasma membrane(PM)and pathogen–plant ***,OsExo70H1 interacted with the rice syntaxin of the plants121 protein(OsSyp121),and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the OsMYA1 and OsExo70H1 ***,these results not only reveal a new function of OsMYA1 in rice blast resistance,but also uncover a molecular mechanism by which plants regulate defense against *** by OsMYA1-initiated vesicle secretory pathway,which originates from the actin cytoskeleton to the PM.

关键词： cytoskeleton plant defense mechanism rice blast vesicle delivery

RACK1A promotes hypocotyl elongation by scaffolding light signaling components in Arabidopsis

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journal of Integrative Plant Biology 2024年第5期66卷 956-972页

作者： Yajuan Fu Wei Zhu Yeling Zhou Yujing Su Zhiyong Li Dayan Zhang Dong Zhang jinyu shen jiansheng Liang Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes Department of BiologySchool of Life SciencesInstitute of Plant and Food SciencesSouthern University of Science and TechnologyShenzhen 518055China

Plants deploy versatile scaffold proteins to intricately modulate complex cell *** these,RACK1A(Receptors for Activated C Kinase 1A)stands out as a multifaceted scaffold protein functioning as a central integrative hub for diverse signaling ***,the precise mechanisms by which RACK1A orchestrates signal transduction to optimize seedling development remain largely ***,we demonstrate that RACK1A facilitates hypocotyl elongation by functioning as a flexible platform that connects multiple key components of light signaling ***1A interacts with PHYTOCHROME INTERACTING FACTOR(PIF)3,enhances PIF3 binding to the promoter of BBX11 and down-regulates its ***,RACK1A associates with ELONGATED HYPOCOTYL 5(HY5)to repress HY5 biochemical activity toward target genes,ultimately contributing to hypocotyl *** darkness,RACK1A is targeted by CONSTITUTIVELY PHOTOMORPHOGENIC(COP)1 upon phosphorylation and subjected to COP1-mediated degradation via the 26 S proteasome *** findings provide new insights into how plants utilize scaffold proteins to regulate hypocotyl elongation,ensuring proper skoto-and photo-morphogenic development.

关键词： Arabidopsis hypocotyl development light signaling components RACK1 scaffoldprotein

The alarming link between neonicotinoid insecticides and kidney injury

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Emerging Contaminants 2024年第3期10卷 318-328页

作者： Zhanpeng Zhang Longbin shen Mei Chen Biao Huang Zhuoqi Wei Weiguo Xu Hua Zhang Department of Metabolic and Bariatric Surgery The First Affiliated Hospital of Jinan UniversityGuangzhou510632China Department of Dermatology The First Affiliated Hospital of Jinan UniversityGuangzhouGuangdong510632China Department of Rehabilitation Medicine The First Affiliated Hospital of Jinan UniversityGuangzhou510632China Department of Interventional The First Affiliated Hospital of Guangzhou University of Traditional Chinese MedicineGuangzhou510400China Zhuhai Interventional Medical Center Zhuhai Precision Medical CenterZhuhai People's HospitalZhuhai Hospital Affiliated with Jinan UniversityZhuhaiGuangdong519000China

Neonicotinoid insecticides(NEOs)have been extensively approved for application in agricultural production both in China and *** studies indicated that human exposure to NEOs would have adverse health ***,studies on occurrences of NEOs in kidney injury patients and their association with nephrotoxicity are *** study investigates the potential correlation between exposure to NEOs and kidney injury within a subset(n=224)of the population residing in South *** of 8 NEOs and 5 metabolites were quantified in blood samples obtained from both the 110 healthy and 114 kidney injury cohorts in South *** found that target analytes were frequently detected in samples from the healthy and kidney injury cohorts(61%-100%).Dinotefuran is the predominant NEO in the healthy(42.3%)and kidney injury cohort(49.6%).Significantly positive(pjury were found,indicating that these pollutants may increase the odds of human kidney injury *** multilinear regression analysis,it was observed that the concentrations of various NEOs exhibited a significant association(pjury,thereby offering novel insights into the nephrotoxicity associated with these *** discovery carries significant implications for public health policies and environmental conservation practices.

关键词： Neonicotinoid insecticides Metabolites Human exposure Kidney injury

Targeted G-to-T base editing for generation of novel herbicide-resistance gene alleles in rice

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journal of Integrative Plant Biology 2024年第6期66卷 1048-1051页

作者： Yifu Tian Xinbo Li jiyong Xie Zai Zheng Rundong shen Xuesong Cao Mugui Wang Chao Dong jian-Kang Zhu Ministry of Agriculture and Rural Affairs Key Laboratory of Gene Editing Technologies(Hainan) Institute of Crop Sciences and National Nanfan Research InstituteChinese Academy of Agricultural SciencesSanya 572024China Hainan Seed Industry Laboratory Sanya 572024China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China Institute of Advanced Biotechnology and School of MedicineSouthern University of Science and TechnologyShenzhen 518055China

Deaminase-based cytosine base editors(CBEs)and adenine base editors(ABEs)induce C-to-T and A-to-G transitions,respectively,enabling single-nucleotide variants(SNVs)in plants for research and crop enhancement(Li et al.,2023a).The C-to-G base editors(CGBEs)and A-to-Y base editors(AYBEs),developed by incorporating glycosylases with CBEs and ABEs,expand the repertoire of base editing products,allowing C-to-T/G and A-to-T/G transversions in plants(Li et al.,2023b,2023c;Sretenovic et a.,2021).

关键词： base alleles CBE

Efficient and multiplex gene upregulation in plants through CRISPR-Cas-mediated knockin of enhancers

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Molecular Plant 2024年第9期17卷 1472-1483页

作者： Qi Yao Rundong shen Yang Shao Yifu Tian Peijin Han Xuening Zhang jian-Kang Zhu Yuming Lu Shanghai Collaborative Innovation Center of Agri-Seeds Joint Center for Single-Cell BiologySchool of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China Shanghai Center for Plant Stress Biology Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of the Chinese Academy of Sciences BeijingChina College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China institute of Advanced Biotechnology and School of Medicine Southern University of Science and TechnologyShenzhen 518055China

Geneupregulation through genome editing is important for plant research and *** insertion of short transcriptional enhancers(STEs)into gene promoters may offer a universal solution akin to transgene-mediated overexpression while avoiding the drawbacks associated with ***,we introduce an“in locus activation”technique in rice that leverages well-characterized STEs for refined,heritable,and multiplexed gene *** address the scarcity of potent enhancers,we developed a large-scale mining approach and discovered a suite of STEs that are capable of enhancing gene expression in rice *** in locus integration of these STEs into eight rice genes resulted in substantial tran-scriptional upregulation in the edited plants,with up to 869.1-fold increases in their transcript ***-ploying a variety of STEs,we achieved delicate control of gene expression,enabling the fine-tuning of key phenotypic traits such as plant *** approach also enabled efficient multiplexed gene upregu-lation,with up to four genes activated simultaneously,significantly enhancing the nicotinamide mononucleotide metabolic ***,heritability studies from the To to T3 generations confirmed the stable and heritable nature of STE-driven gene ***,our work demon-strates that coupled with STE mining,leveraging genome editing for in locus activation and gene upregu-lation holds great promise to be widely adopted in fundamental plant research and crop breeding.

关键词： genome editing short transcriptional enhancers gene upregulation crop breeding

Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

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Chinese journal of Integrative Medicine 2024年第6期30卷 515-524页

作者： CAO Liu-jing LIU Li-ya CHEN You-qin HAN Yu-ying WEI Li-hui YAO Meng-ying FANG Yi WU Mei-zhu CHENG Ying Thomas j.Sferra LIU Hui-xin LI Li PENG jun shen A-ling Clinical Research Institute the Second Affiliated Hospital&Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou350122China Fujian Key Laboratory of Integrative Medicine in Geriatrics Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Pediatrics Case Western Reserve University School of MedicineUH Rainbow Babies and Children’s HospitalClevelandOH44106USA Innovation and Transformation Center Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Health Management Fujian Provincial HospitalShengli Clinical College of Fujian Medical UniversityFuzhou000000China

ObjectiveTo explore the regulatory effect of Pien Tze Huang (PZH) on targeting partner of NOB1 (PNO1) and it’s down-stream mediators in colorectal cancer (CRC) *** polymerase chain reaction was performed to determine mRNA levels of PNO1, TP53, and CDKN1A. Western blotting was performed to determine protein levels of PNO1, p53, and p21. HCT-8 cells were transduced with a lentivirus over-expressing PNO1. Colony formation assay was used to detect cell survival in PNO1 overexpression of HCT-8 cells after PZH treatment. Cell-cycle distribution, cell viability and cell apoptosis were performed to identify the effect of PNO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment. Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PNO1 or sh-Ctrl lentivirus were evaluated. Western blot assay was performed to detect PNO1, p53, p21 and PCNA expression in tumor sections. Terminal deoxynucleotidyl transferase dUTP nick end labling (TUNEL) assay was used to determine the apoptotic cells in *** treatment decreased cell viability, down-regulated PNO1 expression, and up-regulated p53 and p21 expressions in HCT-8 cells (P<0.05). PNO1 overexpression attenuated the effects of PZH treatment, including the expression of p53 and p21, cell growth, cell viability, cell cycle arrest and cell apoptosis in vitro (P<0.05). PNO1 knockdown eliminated the effects of PZH treatment on tumor growth, inhibiting cell proliferation inhibition and apoptosis induction in vivo (P<0.05). Similarly, PNO1 knockdown attenuated the effects of PZH treatment on the down-regulation of PNO1 and up-regulation of p53 and p21 in vivo (P<0.05).ConclusionThe mechanism by which PZH induces its CRC anti-proliferative effect is at least in part by regulating the expression of PNO1 and its downstream targets p53 and p21.

关键词： colorectal cancer Pien Tze Huang Chinese medicine partner of NOB1 proliferation p53/p21 pathway

Different responses of soil fauna gut and plant rhizosphere microbiomes to manure applications

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Soil Ecology Letters 2024年第2期6卷 57-69页

作者： Shuai Du Xiang Zhao Yue Zhang Changlong Shu ju-Pei shen Key Laboratory of Urban Environment and Health Ningbo Urban Environment Observation and Research StationInstitute of Urban EnvironmentChinese Academy of SciencesXiamen361021China School of Geographical Sciences Fujian Normal UniversityFuzhou350007China Institute of Plant Nutrition Resources and EnvironmentBeijing Academy of Agriculture and Forestry SciencesBeijing100097China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193China Hebei Key Laboratory of Soil Entomology Cangzhou Academy of Agricultural and Forestry SciencesCangzhou061001China

Microbial attributes were compared between soil fauna gut and plant rhizosphere.•Manure applications decreased or increased gut or rhizosphere bacterial diversity.•Stochastic or deterministic processes drove gut or rhizosphere bacterial assembly.•Manure applications increased bacterial network complexity of gut and *** microbes inhabit animals and plants,helping their hosts perform multiple functions in agricultural ***,the responses of soil fauna gut and plant rhizosphere microbiomes to livestock manure applications are still not well *** we fed Protaetia brevitarsis larvae(PBL)with chicken manure and collected their *** frass and manure were applied as fertilizers to lettuce *** then compared the changes of microbial diversity,community assembly,and potential functions between the gut group(i.e.,all PBL gut and frass samples)and the rhizosphere group(i.e.,all rhizosphere soil samples).We revealed that manure applications(i.e.,feeding or fertilization)decreased bacterial diversity in the gut group but increased that in the rhizosphere ***,the proportions of Bacilli in the gut group and Gammaproteobacteria in the rhizosphere group were increased(up to a maximum of 33.8%and 20.4%,respectively)after manure *** and deterministic processes dominated community assembly in the gut and rhizosphere microbiomes,*** applications increased the microbial co-occurrence network complexity of both the gut and rhizosphere ***,the proportions of functional taxa associated with human/animal pathogens in the gut group and carbon/nitrogen cycling in the rhizosphere group were enhanced(up to 2.6-fold and 24.6-fold,respectively).Our findings illustrate the different responses of microbial diversity,community assembly,and potential functions in soil fauna gut and plant rhizosphere to manure *** results could enhance our knowledge on the reasonable utilization of

关键词： soil fauna gut plant rhizosphere manure applications community assembly microbial functions