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Synthetic and Systems Biotechnology 2019年第4期4卷 220-224页

作者： j.Porter Hunt Emily Long Zhao Mehran Soltani Madison Frei j.andrew d.nelson Bradley C.Bundy department of Chemical Engineering Brigham Young UniversityProvoUTUSA department of Chemical Engineering Brigham Young University330N Engineering BuildingProvoUT84602USA

An“endotoxin-free”***-based cell-free protein synthesis system has been reported to produce therapeutic proteins rapidly and ***,preparation of the most complex CFPS reagent–the cell extract–remains time-consuming and labor-intensive because of the relatively slow growth kinetics of the endotoxin-free ClearColiTMBL21(dE3)*** we report a streamlined procedure for preparing *** cell extract from ClearColi™using auto-induction *** this work,the term auto-induction describes cell culture media which eliminates the need for manual induction of protein *** Clearcoli™cells in autoinduction media significantly reduces the hands-on time required during extract preparation,and the resulting“endotoxinfree”cell extract maintained the same cell-free protein synthesis capability as extract produced with traditional induction as demonstrated by the high-yield expression of crisantaspase,an FdA approved leukemia *** is anticipated that this work will lower the barrier for researchers to enter the field and use this technology as the method to produce endotoxin-free ***-based extract for CFPS.

关键词： Auto-induction Autoinduction Cell-free protein synthesis CFPS Endotoxin-free Crisantaspase Clearcoli

Addressing nonresponse bias in forest inventory change estimation using response homogeneity classifications

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Forest Ecosystems 2023年第1期10卷 125-131页

作者： james A.Westfall Mark d.nelson U.S.Forest Service Northern Research StationYorkPAUSA U.S.Forest Service Northern Research StationSt.PaulMNUSA

Estimating amounts of change in forest resources over time is a key function of most national forest inventories(NFI). As this information is used broadly for many management and policy purposes, it is imperative that accurate estimations are made from the survey sample. Robust sampling designs are often used to help ensure representation of the population, but often the full sample is unrealized due to hazardous conditions or possibly lack of land access permission. Potentially, bias may be imparted to the sample if the nonresponse is nonrandom with respect to forest characteristics, which becomes more difficult to assess for change estimation methods that require measurements of the same sample plots at two points in time, i.e., remeasurement. To examine potential nonresponse bias in change estimates, two synthetic populations were constructed: 1) a typical NFI population consisting of both forest and nonforest plots, and 2) a population that mimics a large catastrophic disturbance event within a forested population. Comparisons of estimates under various nonresponse scenarios were made using a standard implementation of post-stratified estimation as well as an alternative approach that groups plots having similar response probabilities(response homogeneity). When using the post-stratified estimators, the amount of change was overestimated for the NFI population and was underestimated for the disturbance population, whereas the response homogeneity approach produced nearly unbiased estimates under the assumption of equal response probability within groups. These outcomes suggest that formal strategies may be needed to obtain accurate change estimates in the presence of nonrandom nonresponse.

关键词： disturbance Post-stratification Land use conversion Sample bias

Treating amyotrophic lateral sclerosis with allogeneic Schwann cell-derived exosomal vesicles: a case report

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Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal j.Goldschmidt-Clermont Aisha Khan George jimsheleishvili Patricia Graham Adriana Brooks Risset Silvera Alexander j.P.Goldschmidt damien d.Pearse W.dalton dietrich Allan d.Levi james d.Guest Miller School of Medicine University of MiamiMiamiFLUSA Stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological Surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

Schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,Schwann cell function may also be ***,important signaling and potential trophic activities of Schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FdA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic Schwann cell-derived exosomal vesicles to potentially restore impaired Schwann cell and motor neuron *** confirmed that cultured Schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s Schwann cells to allogeneic Schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating Scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic Schwann cell-derived exosomal vesicles diluted in 40 mL of dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction Schwann cell

Inflammasome links traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease

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Neural Regeneration Research 2025年第6期20卷 1644-1664页

作者： Gabriela Seplovich Yazan Bouchi juan Pablo de Rivero Vaccari jennifer C.Munoz Pareja andrew Reisner Laura Blackwell Yehia Mechref Kevin K.Wang j.Adrian Tyndall Binu Tharakan Firas Kobeissy department of Surgery Morehouse School of MedicineAtlantaGAUSA department of Neurobiology Center for NeurotraumaMultiomics&Biomarkers(CNMB)Neuroscience InstituteMorehouse School of MedicineAtlantaGAUSA department of Neurological Surgery and the Miami Project to Cure Paralysis University of Miami Miller School of MedicineMiamiFLUSA division of Pediatric Critical Care Department of PediatricsUniversity of Miami Miller School of MedicineMiamiFLUSA department of Pediatrics Emory UniversityAtlantaGAUSA department of Neurosurgery Children’s Healthcare of AtlantaAtlantaGAUSA department of Chemistry and Biochemistry Texas Tech UniversityLubbockTXUSA Morehouse School of Medicine AtlantaGAUSA

Traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease are three distinct neurological disorders that share common pathophysiological mechanisms involving neuroinflammation. One sequela of neuroinflammation includes the pathologic hyperphosphorylation of tau protein, an endogenous microtubule-associated protein that protects the integrity of neuronal cytoskeletons. Tau hyperphosphorylation results in protein misfolding and subsequent accumulation of tau tangles forming neurotoxic aggregates. These misfolded proteins are characteristic of traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease and can lead to downstream neuroinflammatory processes, including assembly and activation of the inflammasome complex. Inflammasomes refer to a family of multimeric protein units that, upon activation, release a cascade of signaling molecules resulting in caspase-induced cell death and inflammation mediated by the release of interleukin-1β cytokine. One specific inflammasome, the NOd-like receptor protein 3, has been proposed to be a key regulator of tau phosphorylation where it has been shown that prolonged NOd-like receptor protein 3 activation acts as a causal factor in pathological tau accumulation and spreading. This review begins by describing the epidemiology and pathophysiology of traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease. Next, we highlight neuroinflammation as an overriding theme and discuss the role of the NOd-like receptor protein 3 inflammasome in the formation of tau deposits and how such tauopathic entities spread throughout the brain. We then propose a novel framework linking traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease as inflammasomedependent pathologies that exist along a temporal continuum. Finally, we discuss potential therapeutic targets that may intercept this pathway and ultimately minimize long-term neurological decline.

关键词： Alzheimer's disease caspase-1 chronic traumatic encephalopathy inflammasomes neurodegeneration neuroinflammation NLRP1 NLRP3 pyroptosis tauopathy traumatic brain injury

Tunable broadband two-point-coupled ultra-high-Q visible and near-infrared photonic integrated resonators

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Photonics Research 2024年第9期12卷 1890-1898页

作者： KAIKAI LIU NITESH CHAUHAN MEITING SONG MARK W.HARRINGTON KARL d.nelson dANIEL j.BLUMENTHAL department of Electrical and Computer Engineering University of California Santa BarbaraSanta BarbaraCalifornia 93106USA Honeywell Aerospace PlymouthMinnesota 55441USA

Ultra-high-quality-factor(Q)resonators are a critical component for visible to near-infrared(NIR)applications,including quantum sensing and computation,atomic timekeeping and navigation,precision metrology,microwave photonics,and fiber optic sensing and *** such resonators in an ultra-low-loss CMOS foundry compatible photonic integration platform can enable the transitioning of critical components from the lab-to the chip-scale,such as ultra-low-linewidth lasers,optical reference cavities,scanning spectroscopy,and precision *** optimal operation of these resonators must preserve the ultra-low losses and simultaneously support the desired variations in coupling over a wide range of visible and NIR wavelengths as well as provide tolerance to fabrication *** report a significant advancement in high-performance integrated resonators based on a two-point-coupling design that achieves critical coupling simultaneously at multiple wavelengths across wide wavebands and tuning of the coupling condition at any wavelength,from under-,through critically,to *** demonstrate critical coupling at 698 nm and 780 nm in one visible-wavelength resonator and critical coupling over a wavelength range from 1550 nm to 1630 nm in a 340-million intrinsic Q 10-meter-coil waveguide *** the 340-million intrinsic Q coil resonator,we demonstrate laser stabilization that achieves six orders of magnitude reduction in the semiconductor laser frequency *** also report that this design can be used as a characterization technique to measure the intrinsic waveguide losses from 1300 nm to 1650 nm,resolving hydrogen-related absorption peaks at 1380 nm and 1520 nm in the resonator,giving insight to further reduce waveguide *** CMOS foundry compatibility of this resonator design will provide a path towards scalable system-on-chip integration for high-performance precision experiments and applications,improving reliability,and re

关键词： resonator waveguide tuning

Apples to oranges:environmentally derived,dynamic regulation of serotonin neuron subpopulations in adulthood?

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Neural Regeneration Research 2025年第9期20卷 2596-2597页

作者： Christopher j.O’Connell Matthew j.Robson department of Genetics College of MedicineHarvard UniversityBostonMAUSA james L.Winkle College of Pharmacy Division of Pharmaceutical SciencesUniversity of CincinnatiCincinnatiOHUSA Neuroscience Graduate Program College of MedicineUniversity of CincinnatiCincinnatiOHUSA

Traumatic brain injury(TBI)is a public health problem with an undue economic burden that impacts nearly every age,ethnic,and gender group across the globe(Capizzi et al.,2020).TBIs are often sustained during a dynamic range of exposures to energetic environmental forces and as such outcomes are typically heterogeneous regarding severity and pathology(Capizzi et al.,2020).

关键词： sustained orange dynamic

西藏高原上地幔的精细结构与构造——地震层析成像给出的启示

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地球物理学报 2004年第3期47卷 449-455页

作者：赵文津薛光琦吴珍汉赵逊刘葵史大年 j.Mechie d.nelson L.Brown T.Hearn 中国地质科学院中国地质科学院矿产资源研究所中国地质科学院地质力学研究所 GeoForchungs Zentrum(GFZ) Potsdam14473Germany department of Earth Sciences Syracuse UniversitySyracuseNewYork13244USA department of Geological Sciences Cornell UniversityIthacaNewYork14853USA department of Physics New Mexico State UniversityLasCrucesNM88003USA

利用布置在亚东—格尔木的 1 6 4个流动地震台站记录的 92 6个远震事件的 2 4 2 4 1条射线 ,进行远震P波层析成像处理 ,高分辨率的西藏高原上地幔的速度结构图 ,显示了印度巨厚地幔岩石圈在向高原之下推进的过程中 ,在高喜马拉雅之下拆... 详细信息

利用布置在亚东—格尔木的 1 6 4个流动地震台站记录的 92 6个远震事件的 2 4 2 4 1条射线 ,进行远震P波层析成像处理 ,高分辨率的西藏高原上地幔的速度结构图 ,显示了印度巨厚地幔岩石圈在向高原之下推进的过程中 ,在高喜马拉雅之下拆分成上、下两层 ,这是发生的第一次拆沉 .下层从高喜马拉雅以下约以 2 2°的角度向高原北部插入到 35 0km深 ;而其上层则向北伸展直到雁石坪 ,并构成了高原薄的地幔岩石圈 .在雁石坪北 (33.7°N) ,当其与亚洲大陆岩石圈地幔相遇后发生断离并下沉 .再次证实了五道梁 (35 .2 7°N)深部低速体的存在 ,本区内地壳内低速物质可能与上述运动有联系。

关键词：西藏高原上地幔精细结构构造地震层析成像

Gene therapy for spinal muscular atrophy:perspectives on the possibility of optimizing SMN1 delivery to correct all neurological and systemic perturbations

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Neural Regeneration Research 2025年第7期20卷 2011-2012页

作者： Sharon j.Brown Rafael j.Yáñez-Muñoz Heidi R.Fuller School of Pharmacy and Bioengineering Keele UniversityKeeleUK Wolfson Center for Inherited Neuromuscular disease TORCH BuildingRJAH Orthopaedic HospitalOswestryUK *** Center of Gene and Cell TherapyDepartment of Biological SciencesSchool of Life Sciences and the EnvironmentRoyal Holloway University of LondonEghamUK

Spinal muscular atrophy(SMA)is a genetic condition that results in selective lower motor neuron loss with concomitant muscle weakness and *** genetic cause of SMA was understood in 1995 when loss or impairment of the survival motor neuron 1(SMN1)gene was identified as the main contributing factor(Lefebvre et al.,1995).This,in combination with the discovery that humans have a“back-up”gene,SMN2,which can produce low levels(approximately 10%)of the full-length functional SMN protein,has led to the generation of SMA-specific gene *** was traditionally classified according to age of symptom onset and developmental milestones achieved,with life expectancy and severity varying between ***,SMN2 copy number is used as a proxy for the prediction of disease severity,with higher SMN2 copy number typically being associated with reduced severity of SMA,although this relationship is not absolute:some individuals with low SMN2 copy number have less severe SMA phenotypes and vice ***,the etiology of SMA is further complicated by other factors,such as non-typical nucleotide variants and SMN2-independent modifiers of disease severity.

关键词： atrophy traditionally absolute

Crystalline compounds for remediation of rare-earth fission products:A review

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journal of Rare Earths 2022年第3期40卷 365-380,I0001页

作者： Saehwa Chong Brian j.Riley Zayne j.nelson Pacific Northwest National Laboratory RichlandWA99352USA

Rare earth(RE)-containing crystals have been investigated as media for the immobilization of RE fission products from nuclear waste *** reprocessing of spent nuclear fuel,fission products including REs,alkalis,and alkaline earths released from the *** viable option to immobilize the RE fission products is to incorporate them into chemically durable crystalline phases in specific waste *** study summarizes the crystal structures and synthesis methods of six RE-containing compounds that have applications in remediation of RE fission *** compounds include oxyapatite(AE_(2)RE_(8)(SiO_(4))_(6)O_(2)),oxychloride(REOCl),borosilicate(RE_(3)BSi_(2)O_(10)),pyrochlore(RE_(2)M_(2)O_(7)),monazite(REPO_(4)),and perovskite(REMO_(3))where AE denotes alkaline earth metals and M denotes transition *** review provides an overview of literature on the usage of these six compounds for immobilizing RE fission products and summarizes different synthesis methods for producing these *** of structural parameters with different REs in each compound are also discussed.

关键词： Rare-earth compounds Nuclear waste forms Crystal structures Synthesis of rare-earth compounds

Post electrical or lightning injury syndrome: a proposal for an American Psychiatric Association's diagnostic and Statistical Manual formulation with implications for treatment

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Neural Regeneration Research 2017年第9期12卷 1405-1412页

作者： Christopher j.andrews andrew d.Reisner Mary Ann Cooper Medicine The University of Queensland Lightning and Electrical Injuries Mt Ommaney Family Practice Forensic diagnostic Center of district Nine Lightning Injury Research Program University of Illinois at Chicago African Centres for Lightning and Electromagnetics Network

In the past, victims of electrical and lightning injuries have been assessed in a manner lacking a system- atic formulation, and against ad hoc criteria, particularly in the area of neuropsychological disability. In this manner patients have, for example, only been partially treated, been poorly or incorrectly diagnosed, and have been denied the full benefit of compensation for their injuries. This paper contains a proposal for diagnostic criteria particularly for the neuropsychological aspects of the post injury syndrome. It pays attention to widely published consistent descriptions of the syndrome, and a new cluster analysis of post electrical injury patients. It formulates a proposal which could be incorporated into future editions of the American Psychiatric Association＇s diagnostic and Statistical Manual （dSM）. The major neuropsycholog- ical consequences include neurocognitive dysfunction, and memory subgroup dysfunction, with ongoing consequences, and sometimes including progressive or delayed psychiatric, cognitive, and/or neurological symptoms. The proposed diagnostic criteria insist on a demonstrated context for the injury, both specifying the shock circumstance, and also physical consequences. It allows for a certain delay in onset of symptoms. It recognizes exclusory conditions. The outcome is a proposal for a dSM classification for the post electrical or lightning injury syndrome. This proposal is considered important for grounding patient treatment, and for further treatment trials. Options for treatment in electrical or lightning injury are summarised, and future trials are foreshadowed.

关键词： electrical injury lightning injury neuropsychology neuropsychiatry injury American Psychiatric Association's diagnostic and Statistical Manual