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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop journal 2024年第2期12卷 549-557页

作者： Yan Zheng ei ei Khine Khin Mar Thi ei ei Nyein Likun Huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian Provincial Key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

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Machine learning and bioinformatics to identify biomarkers in response to Burkholderia pseudomallei infection in mice

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BIOCELL 2024年第4期48卷 613-621页

作者： YAO FANG Fei XIA FeiFei TIAN L ei QU FANG YANG jUAN FANG ZHENHONG HU HAICHAO LIU Department of Respiratory and Critical Care Medicine General Hospital of Center Theater of PLAWuhan430070China School of Medicine Wuhan University of Science and TechnologyWuhan430065China School of Life Science and Engineering Southwest Jiaotong UniversityChengdu611756China

Objective:In the realm of Class I pathogens,Burkholderia pseudomallei(BP)stands out for its propensity to induce severe *** the intricate interactions between BP and host cells is imperative for comprehending the dynamics of BP infection and discerning biomarkers indicative of the host cell response ***:mRNA extraction from BP-infected mouse macrophages constituted the initial step of our *** gene expression arrays,the extracted RNA underwent conversion into digital *** percentile shift method facilitated data processing,with the identiﬁcation of genes manifesting signiﬁcant differences accomplished through the application of the ***,a comprehensive analysis involving Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway was conducted on the differentially expressed genes(DEGs).Leveraging the ESTIMATE algorithm,gene signatures were utilized to compute risk scores for gene expression *** vector machine analysis and gene enrichment scores were instrumental in establishing correlations between biomarkers and macrophages,followed by an evaluation of the predictive power of the identiﬁed ***:The functional and pathway associations of the DEGs predominantly centered around G protein-coupled receptors.A noteworthy positive correlation emerged between the blue module,consisting of 416 genes,and the ***4,identiﬁed through support vector machine analysis among intersecting genes,indicated a robust interaction with macrophages,suggesting its potential as a robust ***4 exhibited commendable predictive efﬁcacy,with BP infection inducing its expression in both macrophages and mouse lung *** blotting in macrophages conﬁrmed a signiﬁcant upregulation of FZD4 expression from 0.5 to 24 h *** mouse lung tissue,FZD4 manifested higher expression in the cytoplasm of pulmonary epithelial cells in BP-infected lungs than in the

关键词： Burkholderia pseudomallei Microarray assay Machine learning Bioinformatics FZD4

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Understanding of the Relationship between the Properties of Cu(In,Ga)Se2 Solar Cells and the Structure of Ag Network Electrodes

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ENERGY & ENVIRONMENTAL MATERIALS 2024年第6期7卷 246-253页

作者： Yoo, Hyesun Quy, Hoang Van Lee, Inpyo jo, Seung Taek Hong, Tae ei Kim, junho Yoo, Dae-Hwang Shin, jinwook Commerell, Walter Kim, Dae-Hwan Roh, jong Wook Kyungpook Natl Univ Reg Leading Res Ctr RLRC Smart Energy Syst Daegu 37224 Gyeongsangbug D South Korea Kyungpook Natl Univ Dept Hydrogen & Renewable Energy Daegu 47922 South Korea Kyungpook Natl Univ Dept Nano & Adv Mat Sci & Engn Daegu 37224 Gyeongsangbug D South Korea Daegu Gyeongbuk Inst Sci & Technol DGIST Div Energy Technol Daegu 42988 South Korea Incheon Natl Univ Dept Phys Nano Photoelect Device Lab Incheon 22012 South Korea TH Ulm THU Prittwitzstr 10 D-89081 Ulm Germany

The relation between the structure of the silver network electrodes and the properties of Cu(In,Ga)Se2 (CIGS) solar cells is systemically investigated. The Ag network electrode is deposited onto an Al:ZnO (AZO) thin film, employing a self-forming cracked template. Precise control over the cracked template's structure is achieved through careful adjustment of temperature and humidity. The Ag network electrodes with different coverage areas and network densities are systemically applied to the CIGS solar cells. It is revealed that predominant fill factor (FF) is influenced by the figure of merit of transparent conducting electrodes, rather than sheet resistance, particularly when the coverage area falls within the range of 1.3-5%. Furthermore, a higher network density corresponds to an enhanced FF when the coverage areas of the Ag networks are similar. When utilizing a thinner AZO film, CIGS solar cells with a surface area of 1.0609 cm2 exhibit a notable performance improvement, with efficiency increasing from 10.48% to 11.63%. This enhancement is primarily attributed to the increase in FF from 45% to 65%. These findings underscore the considerable potential for reducing the thickness of the transparent conductive oxide (TCO) in CIGS modules with implications for practical applications in photovoltaic technology.

关键词： CIGS large-area solar cell metal mesh metallic network transparent electrode

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补中益气汤通过Adipor1/AMPK/PGC-1α调节脂代谢治疗运动性疲劳

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中国实验方剂学杂志 2024年第12期30卷 24-30页

作者：彭娟韦华妮姜婷刘伟潘华山陈道睿广州中医药大学体育健康学院广州510006 广州中医药大学岭南医学研究中心广州510405 广东潮州卫生健康职业学院广东潮州521000

目的:探讨补中益气汤通过脂联素受体1(Adipor1)/磷酸腺苷活化蛋白激酶(AMPK)/过氧化物酶体增生物激活受体γ共激活因子-1α(PGC-1α)对运动性疲劳(eiF)小鼠骨骼肌中脂代谢的影响。方法:将C57BL/6j小鼠随机分为空白组、模型组、补中益气... 详细信息

目的:探讨补中益气汤通过脂联素受体1(Adipor1)/磷酸腺苷活化蛋白激酶(AMPK)/过氧化物酶体增生物激活受体γ共激活因子-1α(PGC-1α)对运动性疲劳(eiF)小鼠骨骼肌中脂代谢的影响。方法:将C57BL/6j小鼠随机分为空白组、模型组、补中益气汤低、中、高剂量组、维生素C组。空白组不施加任何干预,其余组通过力竭游泳建立eiF模型。力竭游泳前1 h,空白组、模型组灌服0.2 mL蒸馏水,补中益气汤低、中、高剂量组分别灌胃4.1、8.2、16.4 g·kg^(-1)的补中益气汤,维生素C组灌胃0.04 g·kg^(-1)剂量维生素C,持续6周。实验6周后,计算小鼠体质量增长率、脏器指数、力竭游泳时间;酶比色法检测小鼠血清中尿素氮(BUN)、肌酸激酶(CK)、乳酸脱氢酶(LDH)、乳酸(LD)的水平;苏木素-伊红(HE)染色观察骨骼肌组织病理变化;透射电镜(TEM)观察骨骼肌组织超微结构;微板法检测血清中游离脂肪酸(NEFA)、甘油三酯(TG)的含量;蛋白免疫印迹法(Western blot)测定骨骼肌中Adipor1、磷酸化(p)-AMPK/AMPK、PGC-1α和己糖激酶2(HK2)蛋白的表达。结果:与空白组比较,模型组小鼠的体质量增长率、胸腺指数降低,血清中BUN、CK、LD、LDH水平升高(PeiF的发生,其机制可能与调节Adipor1/AMPK/PGC-1α信号通路,提高骨骼肌对脂肪的利用率有关。

关键词：运动性疲劳补中益气汤骨骼肌脂代谢补中益气汤通过脂联素受体1(Adipor1)/腺苷酸活化蛋白激酶(AMPK)/过氧化物酶体增生物激活受体γ共激活因子-1α(PGC-1α)信号通路

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调质处理对1000 MPa级水电用钢组织性能的影响

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材料热处理学报 2024年第9期45卷 176-183,191页

作者：肖桂枝杨继学安茹马金磊麻衡西安建筑科技大学冶金工程学院陕西西安710055 莱芜钢铁集团银山型钢有限公司山东济南271104

利用扫描电镜、透射电镜、拉伸和冲击试验机等研究了不同调质处理工艺对1000 MPa级水电用钢微观组织和力学性能的影响。结果表明:淬火后的实验钢组织呈准等轴状,板条马氏体组织细小。在890~950℃淬火温度范围内,随着淬火温度的升高,马... 详细信息

利用扫描电镜、透射电镜、拉伸和冲击试验机等研究了不同调质处理工艺对1000 MPa级水电用钢微观组织和力学性能的影响。结果表明:淬火后的实验钢组织呈准等轴状,板条马氏体组织细小。在890~950℃淬火温度范围内,随着淬火温度的升高,马氏体板条束尺寸增加,经过620℃回火后组织转变为回火索氏体,回火过程中碳化物的析出及其与位错的交互作用提高了实验钢的强度。随淬火温度升高,实验钢的屈服强度和抗拉强度增加,伸长率基本保持不变,-60℃冲击吸收能量呈现先缓慢增加后急剧减小的趋势,冲击断裂方式由韧性断裂转变为脆性解理断裂。经920℃×30 min+620℃×60 min调质工艺处理后,实验钢的屈服强度、抗拉强度、伸长率及-60℃冲击吸收能量分别为970 MPa、986 MPa、15%和185 j,其综合力学性能达到最优,满足1000 MPa级水电用钢的性能要求。

关键词：水电用钢调质处理微观结构演化力学性能强化机理

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Maresin-1改善青少年期抑郁小鼠的抑郁样行为和神经炎症

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重庆医科大学学报 2024年第4期49卷 465-470页

作者：陈羽佳石磊徐何雁王余娜杜宁彭志平邱大川夏铸况利重庆医科大学附属第一医院精神科重庆400016 重庆医科大学附属大学城医院心理卫生中心重庆401331 重庆医科大学基础医学院放射医学科重庆400016 重庆医科大学附属第一医院核医学科重庆400016

目的:阐明Maresin-1(MaR1)对慢性社交挫败(chronic social defeated stress,CSDS)诱导青少年期(5~8周)小鼠抑郁样行为的影响,验证其在CSDS诱导的神经炎症中的作用,为理解抑郁症的分子机制和探索生物标志物提供参考。方法:利用多种方法... 详细信息

目的:阐明Maresin-1(MaR1)对慢性社交挫败(chronic social defeated stress,CSDS)诱导青少年期(5~8周)小鼠抑郁样行为的影响,验证其在CSDS诱导的神经炎症中的作用,为理解抑郁症的分子机制和探索生物标志物提供参考。方法:利用多种方法来检测CSDS诱导10 d后C57BL/6j小鼠的抑郁样行为和神经炎症。并每2 d注射1次MaR1(5μg/kg)治疗小鼠,以观察其对CSDS诱导的抑郁样行为和神经炎症的影响。行为学实验后进行PET-CT扫描并对PET图像进行定量分析;收集小鼠脑组织样本进行免疫荧光染色,采用Image j对海马区域Iba-1细胞、TSPO免疫荧光强度进行统计;将小鼠海马体在冰上分离,并立即在液氮中快速冷冻,然后储存在-80℃下进行随后的RNA提取,试剂盒检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素1β(interleukin-1 beta,IL-1β)和IL-4含量。结果:与对照组相比,CSDS应激后,小鼠表现出低糖水偏好比(P=0.003)低社会交互比(P=0.000)、更长的不动时间(P=0.002),在海马区域,小胶质细胞活化,表现为SUV值升高(P=0.020),Iba-1和TSPO的免疫荧光强度增强(P=0.000)和促炎细胞因子IL-1β和TNF-α升高(P=0.016、0.036)。而MaR1改善了上述CSDS诱导的抑郁样行为,抑制小胶质细胞的激活和减少促炎因子的表达。结论:MaR1能够缓解CSDS诱导的青少年期小鼠抑郁样行为,抑制小胶质细胞的活化。神经炎症是青少年抑郁症的潜在致病因素,具有抗炎特性的药物如MaR1有潜力作为临床相关的抗抑郁药,需要进一步地研究。

关键词：青少年抑郁症小胶质细胞 [18F]DPA-714PET Maresin-1

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Peak-to-average power ratio reduction using selective mapping with unequal power distribution

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journal of Central South University 2012年第7期19卷 1902-1908页

作者： E.S.Hassan XU Zhu S.E.E1-Khamy M.I.Dessouky S.A.ei-Dolill F.E.Abd ei-Samie Department of Electronics and Electrical Communications Faculty of Electronic EngineeringMenoufia UniversityMenouf 32952Egypt Department of Electrical Engineering and Electronics The University of LiverpoolLiverpool L693G JUK Department of Electrical Engineering Faculty of EngineeringAlexandria UniversityAlexandria 21544Egypt

A new approach for peak-to-average power ratio (PAPR) reduction in orthogonal frequency division multiplexing (OFDM) systems was *** approach is based on assigning powers to the different subcarriers of OFDM using an unequal power distribution *** addition,a reduced complexity selective mapping (RC-SLM) scheme was *** proposed scheme is based on partitioning the frequency domain symbol sequence into several sub-blocks,and then each sub-block is multiplied by different phase sequences whose length is shorter than that used in the conventional SLM ***,a kind of low complexity conversions is used to replace the IFFT *** performance of the proposed RC-SLM scheme along with the new approach was studied with computer *** obtained results show that the proposed RC-SLM scheme is able to achieve the lowest computational complexity when compared with other low complexity schemes proposed in the literature while at the same time improves the PAPR reduction performance by about 0.3 dB.

关键词： orthogonal frequency division multiplexing (OFDM) peak-to-average power ratio (PAPR) computational complexity selective mapping scheme

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Cadmium nanoclusters in a protein matrix： Synthesis, characterization, and application in targeted drug delivery and cellular imaging

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Nano Research 2016年第11期9卷 3229-3246页

作者： Morteza Sarparast Abolhassan Noori Hoda Ilkhani S. Zahra Bathaie Maher F. ei-Kady Lisa j. Wang Huong Pham Kristofer L. Marsh Richard B. Kaner Mir F. Mousavi Department of Chemistry Tarbiat Modares University Tehran 14115-175 Iran Department of Clinical Biochemistry Faculty of Medical Sciences Tarbiat Modares University Tehran 14115-111 Iran Department of Chemistry and Biochemistry University of California Los Angeles Los Angeles CA 90095 USA Department of Chemistry Faculty of Science Cairo University Giza 12613 Egypt California NanoSystems Institute University of California Los Angeles Los Angeles CA 90095 USA

Biotemplated metal nanoclusters have garnered much attention owing to their wide range of potential applications in biosensing, bioimaging, catalysis, and nanomedicine. Here, we report the synthesis of stable, biocompatible, watersoluble, and highly fluorescent bovine serum albumin-templated cadmium nanoclusters （CdNcs） through a facile one-pot green method. We covalently conjugated hyaluronic acid （HA） to the CdNcs to form a pH-responsive, tumor- targeting theranostic nanocarrier with a sustained release profile for doxorubicin （DOX）, a model anticancer drug. The nanocarrier showed a DOX encapsulation efficiency of about 75.6%. DOX release profiles revealed that 74% of DOX was released at pH 5.3, while less than 26% of DOX was released at pH 7.4 within the same 24-h period. The nanocarrier selectively recognized MCF-7 breast cancer cells expressing CD44, a cell surface receptor for HA, whereas no such recognition was observed with HA receptor-negative HEK293 cells. Biocompatibility of the nanocarrier was evaluated through cytotoxicity assays with HEK293 and MCF-7 ceils. The nanocarrier exhibited very low to no cytotoxicity, whereas the DOX-loaded nanocarrier showed considerable cellular uptake and enhanced MCF-7 breast cancer cell-killing ability. We also confirmed the feasibility of using the highly fluorescent nanoconjugate for bioimaging of MCF-7 and HeLa cells. The superior targeted drug delivery efficacy, cellular imaging capability, and low cytotoxicity position this nanoconjugate as an exciting new nanoplatform with promising biomedical applications.

关键词： Cd nanoclusters hyaluronic acid targeted drug delivery fluorescence bioimaging nanocarrier

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Propofol enhances the field excitatory postsynaptic potentials in CA1 hippocampal slices of young and aged mice

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Chinese Medical journal 2014年第1期127卷 137-141页

作者： Yin Yiqing William j Middleton Carlos M. Florez Peter L. Carlen Hossam ei-Beheiry Department of Anesthesia Peking University China-Japan Friendship Hospital Beijing 100029 China Department of Anesthesia Toronto Westem Hospital University ofToronto University Health Network Toronto Canada Toronto Western Research Institute University of Toronto TorontoCanada Department of Anesthesia Trillium Health Centre MississaugaOntario Canada

Background Increasing age was shown to decrease the requirements for ***,the mechanisms of ageing-induced potentiation of anesthetic actions have not been clearly *** aim of this study is to compare the effects of propofol on the field excitatory postsynaptic potentials （fEPSPs） in hippocampal slices of young and aging *** Brain slices were prepared from C57BL6 male young （2 months） and aging （＞12 months） *** dendritic field excitatory postsynaptic potential was recorded from the CA1 stratum radiatum using patch clamp electrophysiological methods.A bipolar concentric stimulating electrode was placed along the Schaffer collateral for othodromic *** effects of clinically-relevant concentrations of propofol were studied in the young and ageing mouse *** Propofol application increased the orthodromically evoked fEPSP produced in slices taken from young and older animals.A striking feature in the I/O relationship was the decreased enhancement of the fEPSPs by propofol in slices from older mice.A clinically relevant concentration of propofol,10 μmol/L,showed more significant enhancement in amplitude and area under the curve （AUC） of fEPSP in young compared to tissues from older mice （amplitude：young （24.9±3.4）％,old （4.6±1.6）％; AUC young （30.6±5.4）％,old （2.1±1.7）％）.There was no statistically significant difference between the paired-pulse facilitation （PPF） ratios calculated for the responses obtained in tissues from young *** slices from older mice,in the presence of 10 μmol/L propofol,PPF was decreased and returned to baseline after washout （baseline 1.21±0.01,propofol：1.16±0.01）.Bicuculline （15 μmol/L） blocked the enhancement of propofol on fEPSP in tissues from young and old *** The fEPSP of slices from aging mice demonstrates diminished sensitivity to the enhancing actions of propofol.

关键词： propofol field excitatory postsynaptic potentials CA1 hippocampal slice aging patch clamp

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Folded novel accurate analytical and semi-analytical solutions of a generalized Calogero–Bogoyavlenskii–Schiff equation

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Communications in Theoretical Physics 2021年第9期73卷 26-36页

作者： Mostafa M A Khater S K Elagan M A El-Shorbagy S H Alfalqi j F Alzaidi Nawal A Alshehri Department of Mathematics Faculty of ScienceJiangsu University212013ZhenjiangChina Department of Mathematics Obour High Institute For Engineering and Technology11828CairoEgypt Department of Mathematics Faculty of ScienceTaif University P.O.Box 11099Taif 21944Saudi Arabia Department of Mathematics College of Science and Humanities in Al-KharjPrince Sattam bin Abdulaziz UniversityAl-Kharj 11942Saudi Arabia Department of Basic Engineering Science Faculty of EngineeringMenoufia UniversityShebin El-Kom 32511Egypt Department of Mathematics Faculty of Science and ArtsMahayil Asir King Khalid UniversityAbhaSaudi Arabia

This paper studies the analytical and semi-analytic solutions of the generalized Calogero–Bogoyavlenskii–Schiff(CBS)*** model describes the(2+1)–dimensional interaction between Riemann-wave propagation along the y-axis and the x-axis *** extended simplest equation(ESE)method is applied to the model,and a variety of novel solitarywave solutions is *** solitary-wave solutions prove the dynamic behavior of soliton waves in *** accuracy of the obtained solution is verified using a variational iteration(VI)semi-analytical *** analysis and the match between the constructed analytical solution and the semi-analytical solution are sketched using various diagrams to show the accuracy of the solution we *** adopted scheme’s performance shows the effectiveness of the method and its ability to be applied to various nonlinear evolution equations.

关键词： generalized Calogero-Bogoyavlenskii-Schiff equation accurate analytical semianalytical solutions

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