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Generation of Eco-Friendly and Disease-Resistant Channel Catfish(Ictalurus punctatus)Harboring the Alligator Cathelicidin Gene via CRISPR/Cas9 Engineering

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Engineering 2024年第8期39卷 273-286页

作者： jinhai Wang Baofeng Su De xing Timothy j.Bruce Shangj.a Li Logan Bern Mei Shang Andrew johnson Rhoda Mae C.Simora Michael Coogan Darshika U.Hettiarachchi Wenwen Wang Tasnuba Hasin jacob Al-Armanazi Cuiyu Lu Rex A.Dunham School of Fisheries Aquacultureand Aquatic SciencesAuburn UniversityAuburnAL 36849USA

As a precise and versatile tool for genome manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)platform holds promise for modifying fish traits of *** the aim of reducing transgene introgression and controlling reproduction,upscaled disease resistance and reproductive intervention in catfish species have been studied to lower the potential environmental risks of the introgression of escapees as transgenic *** advantage of the CRISPR/Cas9-mediated system,we succeeded in integrating the cathelicidin gene(As-Cath)from an alligator(Alligator sinensis)into the target luteinizing hormone(lh)locus of channel catfish(Ictalurus punctatus)using two delivery systems assisted by double-stranded DNA(dsDNA)and single-stranded oligodeoxynucleotides(ssODNs),*** this study,high knock in(KI)efficiency(22.38%,64/286)but low ontarget events was achieved using the ssODN strategy,whereas adopting a dsDNA as the donor template led to an efficient on-target KI(10.80%,23/213).The on-target KI of As-Cath was instrumental in establishing the lh knockout(LH^(–)_As-Cath^(+))catfish line,which displayed heightened disease resistance and reduced fecundity compared with the wild-type(WT)sibling ***,administration of human chorionic gonadotropin(HCG)and luteinizing hormone-releasing hormone analogue(LHRHa)can restore the reproduction of the transgenic fish ***,we replaced the lh gene with an alligator cathelicidin transgene and then administered hormone therapy to move towards complete reproductive control of diseaseresistant transgenic catfish in an environmentally responsible *** strategy not only effectively improves consumer-valued traits but also guards against unwanted introgression,providing a breakthrough in aquaculture genetics to confine fish reproduction and prevent the establishment of transgenic or domestic genotypes in the natural environment.

关键词： Genome editing ssODN dsDNA Antimicrobial peptide Reproductive confinement Aquaculture

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Ordered Bicontinuous Network Structures Regulated by Orientational Interactions in a Rod-Coil Block Copolymer

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Chinese journal of Polymer Science 2024年第5期42卷 636-642,I0008页

作者： Xiao-Lin Lyu Shi-Chu Yang An-Qi Xiao Ping-Ping Hou Wei Zhang Hong-Bing Pan Zhi-Hao Shen xing-He Fan Qi-Feng Zhou Beijing National Laboratory for Molecular Sciences Key Laboratory of Polymer Chemistry and Physics of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing100871China Key Laboratory of Advanced Materials Technologies International(HongKong Macao and Taiwan)Joint Laboratory on Advanced Materials TechnologiesCollege of Materials Science and EngineeringFuzhou UniversityFuzhou350108China

The rich phase behavior of block copolymers(BCPs)has drawn great attention in recent ***,the double diamond(DD)phase is rarely obtained because of the competition between the minimization of interfacial energy and packing ***,a rod-coil BCP containing mesogen-jacketed liquid crystalline polymer is designed to acquire ordered bicontinuous network *** reduction of internal energy originating from the orientational interaction among the rod blocks can compensate for the free energy penalty of packing frustration to stabilize the DD *** resulting BCP can also experience lamellae-to-DD and double gyroid-to-lamellae transitions by changing the annealing *** results make the rod-coil BCP an excellent candidate for the self-assembly of ordered network structures,demonstrating great potential in nanopatterning and metamaterials.

关键词： Block copolymer Rod-coil Ordered bicontinuous phase Double diamond Double gyroid

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Magnetocaloric effect and phase transition critical behavior of La0.75Sr0.25Mn0.9Co0.1O3 compound synthesized under the high pressure

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Tungsten 2024年第3期6卷 621-632页

作者： jing Zhao Lei Gao j.an-jun Zhao Wei Wei Hui-Qin Yun Ru xing Huai-jin Ma Xiang jin Luo-Meng Chao School of Physical Science and Technology Key Laboratory of Magnetism and Magnetic Materials for Higher Education in Inner Mongolia Autonomous Region Baotou Teachers' College National Key Laboratory of Baiyunobo Rare Earth Resource Research and Comprehensive Utilization Baotou Research Institute of Rare Earths School of Physics and Electronic Information Inner Mongolia Normal University College of Science Inner Mongolia University of Science and Technology

In this study,a novel method is proposed for producing perovskite rare-earth manganese oxide magnetic refrigeration materials that exhibit superior properties compared to similar *** maximum magnetic entropy change values for this sample are 1.44 and 3.75 j·kg-1·K-1for magnetic fields of 2 and 7 T,***,its relative cooling power has been calculated to be 101.96 and 404.78 j·kg-1,indicating good *** results were obtained using the sample synthesized under a pressure of 4 GPa,and the sample has undergone a second phase *** critical behavior of the sample fits well with the mean field *** different magnetic fields,there is a significant overlap between the values of the maximum magnetic entropy change and the temperature-averaged entropy change,indicating that the refrigeration performance of the sample after high pressure can approach its maximum capability.

关键词： High-pressure synthesis Magnetocaloric effect Relative cooling power Magnetic phase transition

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Single pair of charge-2 Dirac and charge-2Weyl phonons in GeO_(2)

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Chinese Physics B 2024年第10期33卷 141-145页

作者： Dong-Chang He j.a-Xi Liu Pei-Tao Liu j.ang-Xu Li xing-Qiu Chen Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China

The presence of a pair of Weyl and Dirac points(WP-DP)in topological semimetal states is intriguing and sought after due to the effects associated with chiral topological ***,identifying these states in real materials poses a significant *** this study,by means of first-principles calculations we predict the coexistence of charge-2 Dirac and charge-2 Weyl phonons at high-symmetry points within a noncentrosymmetric P4_(1)2_(1)2 space ***,we propose GeO_(2)as an ideal candidate for realizing these ***,we observe two distinct surface arcs that connect the Dirac and Weyl points across the entire Brillouin zone,which could facilitate their detection in future experimental *** study not only presents a tangible material for experimentalists to explore the topological properties of WP-DP states but also opens up new avenues in the quest for ideal platforms to study chiral particles.

关键词： ological phonon Weyl points Dirac points

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The siphonic energy transfer between hot solar wind and cold martian ionosphere through open magnetic flux rope

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Fundamental Research 2024年第4期4卷 882-889页

作者： Xiaojun Xu Lou-Chuang Lee Qi Xu Qing Chang jing Wang Ming Wang Shaosui Xu Christian Mosti Charles j.Farrugia xing Wang Yudong Ye Zilu Zhou Lei Luo Peishan He Shaoguan Cheng State Key Laboratory of Lunar and Planetary Sciences Macao University of Science and TechnologyMacao 999078China CNSA Macao Center for Space Exploration and Science Macao 999078China Institute of Earth Science Academia SinicaTaipei 115029China Institute of Space Science and Applied Technology Harbin Institute of TechnologyShenzhen 518000China Planetary Environmental and Astrobiological Research Laboratory(PEARL) School of Atmospheric SciencesSun Yat-sen UniversityZhuhai 519000China Nanjing University of Information Science and Technology Nanjing 210000China Space ScienceLaboratory Universityof CaliforniaBerkeleyCA94320USA Space Research Institute Austrian Academy of SciencesGraz 8010Austria University of New Hampshire NH 03824USA

A mechanism for energy transfer from the solar wind to the Martian ionosphere through open magnetic flux rope is proposed based on the observations by Mars Atmosphere and Volatile EvolutioN(MAVEN).The satellite was located in the dayside magnetosheath at an altitude of about 70o km above the northern *** between the hot solar wind protons and the cold heavy ions/neutrals in the subsolar region can cool the protons and heat the heavy *** a result,the magnetosheath protons are siphoned into the ionosphere due to the thermal pressure gradient of protons and the heated heavy ions escape along the open magnetic field *** direct collisions in the lower-altitude region were not detected,this physical process is demonstrated by MAVEN measurements of enhanced proton density,decreased proton temperature and oppositely directed motions of hot and cool protons within the flux rope,which are very different from the observational features of the flux transfer events near the Earth's *** mechanism could universally exist in many contexts where a collisionless plasma region is connected to a collisional plasma *** reconstructing the magnetic geometry and the cross-section of the flux rope using the Grad-Shafranov technique,the ion loss rates are quantitatively estimated to be on the order of 1023 s-l,which is much higher than previously estimated.

关键词： Mars Flux rope Solarwind Ionosphere Ion loss

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Bio-ecological remediation of freshwater aquaculture environments:A systematic review and bibliometric analysis

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Water Biology and Security 2024年第1期3卷 11-22页

作者： Qiaohong Zhou Kaixuan Wu Lu Yao Ruonan Chen Shouzhuang Liu Hao xing Linmei Nie Zhenbin Wu State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesNo.7 Donghu South RoadWuchang DistrictWuhan430072China University of Chinese Academy of Sciences 19 A Yuquan RoadShijingshan DistrictBeijing100049China China University of Geosciences No.388 Lumo RoadHongshan DistrictWuhan430074China Centre for Sustainable Development and Innovation of Water Technology Foundation CSDI WaterTechForskningsveien 10373OsloNorway

Freshwater aquaculture plays an important role in the global aquaculture *** success of freshwater aquaculture relies on the integration of bio-ecological remediation technologies to ensure its sustainable *** paper systematically reviews the global literatures on bio-ecological remediation of freshwater aquaculture environments from 1990 to 2021,and discusses in detail the research progress and status quo in this field using both bibliometric analysis and content *** results indicate that the number of publications per year has increased year by year,with a particularly rapid increase after *** and researchers from China and the USA publish the greatest number of papers and contribute the most to this *** analyzing the research progress in this field,we also find that the protection and restoration of freshwater aquaculture environments has received attention since as early as 1990,such as the application of constructed wetlands and recirculating aquaculture ***,researchers have increasingly focused on the use of microbial communities in ***,vanRijn,j's papers are of great value to the research in this *** content analysis,the application of primary technologies,including plant selection,aquatic animals,microorganisms,and biological filtration and purification equipment,as well as ecological engineering technologies with combinations of various types of organisms,is described to improve the effectiveness and efficiency of *** performance,advantages,disadvantages,or limitations and prospects of these technology applications are analyzed in *** comparison,ecological engineering is shown to be the best remediation technology,being most comprehensive,and *** together,this paper composes and summarizes existing work in this field,and provides guidance for future research on the improvement of existing technologies and the development of new techno

关键词： Freshwater aquaculture environment Bio-ecological remediation technology Systematic review Bibliometric analysis Content analysis

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The de Haas-van Alphen quantum oscillations in the kagome metal RbTi_(3)Bi_(5)

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Chinese Physics B 2024年第10期33卷 356-361页

作者： Zixian Dong Lei Shi Bin Wang Mengwu Huo xing Huang Chaoxin Huang Peiyue Ma Yunwei Zhang Bing Shen Meng Wang Center for Neutron Science and Technology Guangdong Provincial Key Laboratory of Magnetoelectric Physics and DevicesSchool of PhysicsSun Yat-Sen UniversityGuangzhou 510275China

The kagome system has attracted great interest in condensed matter physics due to its unique structure that canhost various exotic states such as superconductivity(SC),charge density waves(CDWs)and nontrivial topological *** topological semimetal RbTi_(3)Bi_(5)consisting of a Ti kagome layer shares a similar crystal structure to the topologicalcorrelated materials AV_(3)Sb_(5)(A=K,Rb,Cs)but without the absence of CDW and *** de Haas-van Alphenoscillation measurements are performed on single crystals of RbTi_(3)Bi_(5)to pursue nontrivial topological physics and *** this with theoretical calculations,the detailed Fermi surface topology and band structure are investigated.A two-dimensional Fermi pocket b is revealed with a light effective mass,consistent with the semimetal *** fan diagram of RbTi_(3)Bi_(5)reveals a zero Berry phase for the b oscillation in contrast to that of CsTi_(3)Bi_(5).Theseresults suggest that kagome RbTi_(3)Bi_(5)is a good candidate for exploring nontrivial topological exotic states and topologicalcorrelated physics.

关键词： oscillation Fermi surface band structure

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Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A

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journal of Integrative Plant Biology 2024年第9期66卷 1915-1933页

作者： Miqi Xu Yi‐Yuan Wang Yujie Wu Xiuhong Zhou Ziyan Shan Kunying Tao Kaiqiang Qian Xuncheng Wang j.an Li Qingqing Wu xing Wang Deng jun‐jie Ling National Engineering Laboratory of Crop Stress Resistance Breeding School of Life SciencesAnhui Agricultural UniversityHefei 230036China Province Key Laboratory of Resource Insect Biology and Innovative Utilization School of Life SciencesAnhui Agricultural UniversityHefei 230036China Biotechnology Center State Key Laboratory of Tea Plant Biology and UtilizationSchool of Tea and Food Sciences and TechnologyAnhui Agricultural UniversityHefei 230036China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China College of Life Sciences Nanjing Normal UniversityNanjing 210023China State Key Laboratory of Protein and Plant Gene Research Peking–Tsinghua Center for Life SciencesSchool of Advanced Agriculture Sciencesand School of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory of Wheat Improvement Shandong Laboratory of Advanced Agricultural Sciences at WeifangPeking University Institute of Advanced Agricultural SciencesWeifang 261000China

Although green light(GL)is located in the middle of the visible light spectrum and regulates a series of plant developmental processes,the mechanism by which it regulates seedling development is largely *** this study,we demonstrated that GL promotes atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochrome B(phyB)and *** the Pr-to-Pfr conversion rates of phyB and phyA under GL were lower than those under red light(RL)in a fluence rate-dependent and time-dependent manner,long-term treatment with GL induced high Pfr/Pr ratios of phyB and ***,GL induced the formation of numerous small phyB photobodies in the nucleus,resulting in atypical photomorphogenesis,with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to *** abundance of phyA significantly decreased after short-and long-term GL *** determined that four major PHYTOCHROME-INTERACTING FACTORs(PIFs:PIF1,PIF3,PIF4,and PIF5)act downstream of phyB in GL-mediated cotyledon *** addition,GL plays opposite roles in regulating different *** example,under continuous GL,the protein levels of all PIFs decreased,whereas the transcript levels of PIF4 and PIF5 strongly increased compared with dark *** together,our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL-mediated atypical photomorphogenesis.

关键词： cotyledon development green light photobody phytochrome PIF3

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Advancing Energy Systems with In-Situ and Operando Surface-Enhanced Raman Scattering Spectroscopy

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CCS Chemistry 2024年第6期6卷 1403-1421页

作者： Haoming Bao Shi Xuan Leong jaslyn Ru Ting Chen Zhenhai Shi Suli Chen Yan Lv Tianxi Liu In Yee Phang xing Yi Ling Key Laboratory of Synthetic and Biological Colloids Ministry of EducationInternational Joint Research Laboratory for Nano Energy CompositesSchool of Chemical and Material EngineeringJiangnan UniversityWuxi 214122 School of Chemistry Chemical Engineering and BiotechnologyNanyang Technological UniversitySingapore 637371

Surface-enhanced Raman scattering spectroscopy(SERS)has emerged as a powerful analytical technique to enable nanoscale investigations of energy *** mini-review focuses on the applications of in-situ and operando SERS in energy-related research,highlighting its unique capabilities and significant contributions to understanding energy storage and conversion *** first introduce the fundamental principles of SERS,key SERS-derived techniques,and commonly employed ***,we delve into the diverse applications of in-situ and operando SERS across various energy systems,encompassing photocatalytic and electrocatalytic systems,fuel cells,solar cells,and ***,we conclude with our perspective on the current challenges and prospects in this *** hope thismini-review serves as an essential overview to guide the design and implementation of in-situ and operando SERS studies of energy systems.

关键词： surface-enhanced Raman scattering spectroscopy in-situ operando energy systems photocatalysis electrocatalysis fuel cells solar cells batteries

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Structural transition,electric transport,and electronic structures in the compressed trilayer nickelate La_(4)Ni_(3)O_(10)

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Science China(Physics,Mechanics & Astronomy) 2024年第11期67卷 9-15页

作者： jingyuan Li Cui-Qun Chen Chaoxin Huang Yifeng Han Mengwu Huo xing Huang Peiyue Ma Zhengyang Qiu junfeng Chen Xunwu Hu Lan Chen Tao Xie Bing Shen Hualei Sun Dao-Xin Yao Meng Wang Center for Neutron Science and Technology Guangdong Provincial Key Laboratory of Magnetoelectric Physics and DevicesSchool of PhysicsSun Yat-sen UniversityGuangzhou 510275China Center for Materials of the Universe School of Molecular SciencesArizona State UniversityTempe 85287USA School of Sciences Sun Yat-sen UniversityShenzhen 518107China

Atomic structure and electronic band structure are fundamental properties for understanding the mechanism of superconductivity. Motivated by the discovery of pressure-induced high-temperature superconductivity at 80 K in the bilayer Ruddlesden-Popper nickelate La_(3)Ni_(2)O_(7), the atomic structure and electronic band structure of the trilayer nickelate La_(4)Ni_(3)O_(10) under pressure up to 44.3 GPa are investigated. A structural transition from the monoclinic P2_(1)/a space group to the tetragonal I4/mmm around 12.6-13.4 GPa is identified, accompanied by a drop of resistance below 7 K. Density functional theory calculations suggest that the bonding state of Ni 3d_(z^(2)) orbital rises and crosses the Fermi level at high pressures, which may give rise to possible superconductivity observed in resistance under pressure in La_(4)Ni_(3)O_(10). The trilayer nickelate La_(4)Ni_(3)O_(10) shows some similarities with the bilayer La_(3)Ni_(2)O_(7) and has unique properties, providing a new platform to investigate the underlying mechanism of superconductivity in nickelates.

关键词： La_(4)Ni_(3)O_(10) high pressure synchrotron X-ray diffraction structural transition DFT calculations

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