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Energy and Built Environment 2020年第4期1卷 417-422页

作者： Z.jiao j.yuan C.Farnham K.Emura Dept.of Housing and Environmental Design Graduate School of Human Life ScienceOsaka City UniversityOsaka 558-8585Japan Dept.of Architecture and Civil Engineering Toyohashi University of Technology1-1 HibarigaokaTempaku-choToyohashiAichi 441-8580Japan

In japan,in order to determine the capacity of air-conditioning equipment,designers usually use the weather database of Reference Weather Year(RWY)to obtain the design air-conditioning load by using software such as New HASP/ACLD and Building Energy Simulation Tool(BEST)that are often used in *** recent years,with the global warming due to climate change,the weather database used to calculate air conditioning load also ***,in order to determine an appropriate capacity of air-conditioning equipment for energy conservation of buildings,the deviation of design air-conditioning load calculated using the weather database of RWY and Actual Weather Year(AWY)should be *** this paper,New HASP/ACLD was used to calculate the building heat loads of eight major japanese cities over 30 years(1981-2010)between RWY and *** heat load at an exceedance probability of 2.5%is defined as the design air-conditioning load in this *** the design air-conditioning load obtained from RWY and AWY,it is shown that it is not necessarily the most appropriate when using the RWY to calculate the design air-conditioning load,especially for heating load in ***,it is also shown that the annual heating load time ratio has decreased and the annual cooling load time ratio has increased over the 30 years.

关键词： Design air-conditioning load Annual air-conditioning load time ratio Reference weather year Actual weather year New HASP/ACLD

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超声波在轮轨材料中传播的数值模拟

西南交通大学学报 2012年第5期47卷 836-841,848页

作者：邓维礼永远西南交通大学材料科学与工程学院四川成都610031

为进一步研究超声波的声场分布特性,增强超声波在高速铁路中轮轨探伤、列车安全监测等方面的应用,设计了轮轨有限元模型,模拟了不同声源尺寸下不同频率超声波在轮轨材料中传播时的声场分布,绘制了声源轴线上的声压分布曲线,在此基础上,... 详细信息

为进一步研究超声波的声场分布特性,增强超声波在高速铁路中轮轨探伤、列车安全监测等方面的应用,设计了轮轨有限元模型,模拟了不同声源尺寸下不同频率超声波在轮轨材料中传播时的声场分布,绘制了声源轴线上的声压分布曲线,在此基础上,分析了超声波近场长度、指向性、超声波频率及声源大小之间的关系.研究结果表明:在相同声源直径的模型中,频率为1.0 MHz的超声波近场长度最长,指向性最好;相同超声频率的模型中,声源直径为40 mm的超声波近场长度最长,指向性最好.

关键词：超声波传播轮轨数值模拟

Programmable endonuclease combined with isothermal polymerase amplification to selectively enrich for rare mutant allele fractions

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Chinese Chemical Letters 2022年第8期33卷 4126-4132页

作者： junman Chen Tian Qiu Michael G.Mauk Zheng Su Yaguang Fan Dennis j.yuan Qinghua Zhou Youlin Qiao Haim H.Bau jianming Ying jinzhao Song Key Laboratory of Clinical Laboratory Diagnostics(Ministry of Education) College of Laboratory MedicineChongqing Medical UniversityChongqing 400016China The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhou 310022China Department of Mechanical Engineering and Applied Mechanics University of PennsylvaniaPhiladelphiaPA 19104United States Department of Pathology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Center for Global Health School of Population Medicine and Public HealthChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment Tianjin Lung Cancer InstituteTianjin Medical University General HospitalTianjin 300052China Sichuan Lung Cancer Institute Sichuan Lung Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China

Liquid biopsy is a highly promising method for non-invasive detection of tumor-associated nucleic acid fragments in body fluids but is challenged by the low abundance of nucleic acids of clinical interest and their sequence homology with the vast background of nucleic acids from healthy ***,programmable endonucleases such as clustered regularly interspaced short palindromic repeats(CRISPR)associated protein(Cas)and prokaryotic Argonautes have been successfully used to remove background nucleic acids and enrich mutant allele fractions,enabling their detection with deep next generation sequencing(NGS).However,the enrichment level achievable with these assays is limited by futile binding events and off-target *** overcome these shortcomings,we conceived a new assay(Programmable Enzyme-Assisted Selective Exponential Amplification,PASEA)that combines the cleavage of wild type alleles with concurrent polymerase *** PASEA increases the numbers of both wild type and mutant alleles,the numbers of mutant alleles increase at much greater rates,allowing PASEA to achieve an unprecedented level of selective enrichment of targeted *** combining CRISPR-Cas9 based cleavage with recombinase polymerase amplification,we converted samples with0.01%somatic mutant allele fractions(MAFs)to products with 70%MAFs in a single step within 20 min,enabling inexpensive,rapid genotyping with such as Sanger ***,PASEA's extraordinary efficiency facilitates sensitive real-time detection of somatic mutant alleles at the point of care with custom designed Exo-RPA ***-time PASEA'performance was proved equivalent to clinical amplification refractory mutation system(ARMS)-PCR and NGS when testing over hundred cancer patients'*** strategy has the potential to reduce the cost and time of cancer screening and genotyping,and to enable targeted therapies in resource-limited settings.

关键词： Mutant allele enrichment Programmable endonuclease Liquid biopsy Mutation detection Point-of-care testing CRISPR-Cas9 Recombinase polymerase amplification Nucleic acid diagnostics

SARS-CoV-2 spike protein receptor-binding domain perturbates intracellular calcium homeostasis and impairs pulmonary vascular endothelial cells

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Signal Transduction and Targeted Therapy 2023年第8期8卷 3780-3793页

作者： Kai Yang Shiyun Liu Han Yan Wenju Lu Xiaoqian Shan Haixia Chen Changlei Bao Huazhuo Feng jing Liao Shuxin Liang Lei Xu Haiyang Tang jason X-j.yuan Nanshan Zhong jian Wang State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangdong Key Laboratory of Vascular DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina Guangzhou National Laboratory Guangzhou International Bio IslandGuangzhouGuangdongChina Department of Pulmonary and Critical Care Medicine The Affiliated Hospital of Inner Mongolia Medical UniversityHohhotInner Mongolia Autonomous RegionChina Department of Pathology The Affiliated Cancer Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina School of Medicine Southern University of Science and TechnologyShenzhenGuangdongChina Section of Physiology Division of PulmonaryCritical Care and Sleep MedicineDepartment of MedicineUniversity of California San DiegoLa JollaCAUSA

Exposure to the spike protein or receptor-binding domain(S-RBD)of SARS-CoV-2 significantly influences endothelial cells and induces pulmonary vascular *** this study,angiotensin-converting enzyme 2 humanized inbred(hACE2 Tg)mice and cultured pulmonary vascular endothelial cells were used to investigate how spike protein/S-RBD impacts pulmonary vascular *** show that S-RBD leads to acute-to-prolonged induction of the intracellular free calcium concentration([Ca^(2+)]i)via acute activation of TRPV4,and prolonged upregulation of mechanosensitive channel Piezo1 and store-operated calcium channel(SOCC)key component Orai1 in cultured human pulmonary arterial endothelial cells(PAECs).In mechanism,S-RBD interacts with ACE2 to induce formation of clusters involving Orai1,Piezo1 and TRPC1,facilitate the channel activation of Piezo1 and SOCC,and lead to elevated *** effects are blocked by Kobophenol A,which inhibits the binding between S-RBD and ACE2,or intracellular calcium chelator,*** of Piezo1 and SOCC by GsMTx4 effectively protects the S-RBDinduced pulmonary microvascular endothelial damage in hACE2 Tg mice via normalizing the elevated[Ca^(2+)]*** to prototypic strain,Omicron variants(BA.5.2 and XBB)of S-RBD induces significantly less severe cell *** analysis indicates that prototypic S-RBD confers more severe acute impacts than Delta or Lambda *** summary,this study provides compelling evidence that S-RBD could induce persistent pulmonary vascular endothelial damage by binding to ACE2 and triggering[Ca^(2+)]i through upregulation of Piezo1 and *** inhibition of ACE2-Piezo1/SOCC-[Ca^(2+)]i axis proves a powerful strategy to treat S-RBD-induced pulmonary vascular diseases.

关键词： ACE2 elevated homeostasis

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基于时频域特征分析的列车轴承缺陷实时检测

西南交通大学学报 2017年第6期52卷 1182-1187页

作者：永远黄小霞唐媛恬西南交通大学材料科学与工程学院四川成都610031

为对列车轴承状态的实时监测和缺陷的快速诊断,提出了一种基于时频域特征分析的检测方法.该方法结合时域特征分析和基于小波变换的共振解调法,建立包括传感器数据采集模块、异音诊断模块、数据存储和输出模块的列车轴承缺陷实时检测系统... 详细信息

为对列车轴承状态的实时监测和缺陷的快速诊断,提出了一种基于时频域特征分析的检测方法.该方法结合时域特征分析和基于小波变换的共振解调法,建立包括传感器数据采集模块、异音诊断模块、数据存储和输出模块的列车轴承缺陷实时检测系统.利用该系统分别对正常轴承、内圈缺陷轴承以及滚子缺陷轴承进行了现场测试.结果表明:时域特征分析可初步诊断出轴承是否存在缺陷;基于小波变换的共振解调方法提取列车轴承其内圈缺陷频率为35 Hz,滚子缺陷频率为23 Hz,验证了该实时检测系统的稳定性和可靠性.

关键词：列车轴承缺陷实时检测时频域特征

在两个大豆重组自交系群体中分离出的控制产量和抗病性的数量性状位点

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国外作物育种 2002年第4期21卷 57-58页

作者： j.yuan 向平

Preparation,characteristics and water splitting performance of oxynitride photocatalysts:LnTaOxNy and Ln3TaOxNy(Ln=Sc,Y,La,Pr,Eu,Gd)

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Preparation,characteristics and water splitting performance ...

性别对小鼠慢性低氧性肺动脉高压及肺动脉血管活性的影响

第九届全国氢能学术会议

作者： W.j.Xiao,j.yuan,M.X.Chen,j.W.Shi and W.F.Shangguan Shanghai jiao Tong University,China

The development of visible-light-driven photocatalyst has attracted much attention these years because it offers great potential for effective utilizing of the clean and renewable solar energy for decomposing pollutants and energy *** Asahi et al reported the nitrogen-doped TiO displaying high photocatalytic reactivity under visible light in 2001,

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中华医学杂志 2014年第22期94卷 1692-1695页

作者：万钧 jason X. j.yuan 缪冉翟振国张明园杨媛华王军王辰北京呼吸疾病研究所北京市呼吸与肺循环疾病重点实验室首都医科大学附属北京朝阳医院呼吸与危重症医学科 100020 美国伊利诺伊大学医学院首都医科大学基础医学院生理学教研室

目的：探讨性别对小鼠慢性低氧性肺动脉高压及肺动脉血管活性的影响。方法8周龄小鼠雌雄各30只，在雌雄两组中按随机数字表法随机分为常氧组与低氧组各15只，低氧组置于低氧舱（10％O2）中，4周后测定右心室收缩压与右心肥大指数，分离... 详细信息

目的：探讨性别对小鼠慢性低氧性肺动脉高压及肺动脉血管活性的影响。方法8周龄小鼠雌雄各30只，在雌雄两组中按随机数字表法随机分为常氧组与低氧组各15只，低氧组置于低氧舱（10％O2）中，4周后测定右心室收缩压与右心肥大指数，分离肺动脉环，观察高钾及U-46619诱发的肺动脉收缩强度，比较雌、雄小鼠低氧与常氧组间的差异。结果慢性低氧诱发了小鼠肺动脉高压，雌性和雄性常氧组右心室收缩压均显著低于同性别低氧组[（23.40±0.16）和（24.71±0.44）比（31.46±2.33）和（33.66±1.38） mmHg（1 mmHg＝0.133 kPa），P＝0.029和P＜0.001]；雌性和雄性常氧组右心肥大指数均显著低于同性别低氧组（0.260±0.015和0.272±0.007比0.354±0.012和0.367±0.012，P＝0.001和P＜0.001）；而雌性低氧组与雄性低氧组右心室收缩压、右心肥大指数差异均无统计学意义（ P＝0.447、0.471）。高钾、U-46619诱导的肺动脉血管收缩呈钙依赖性，撤除细胞外钙明显抑制血管环收缩。慢性低氧可显著增强高钾、U-46619诱发的肺动脉血管环收缩，收缩强度呈浓度依赖性，但在高钾、U-46619引起的收缩强度在雌、雄低氧组间差异均无统计学意义（P＝0.657、0.751）。结论性别与小鼠慢性低氧性肺动脉高压的发病、肺动脉高压的严重程度以及慢性低氧中的肺动脉血管活性均无显著相关性。

关键词：性别特性高血压,肺性血管活性缺氧小鼠

Relativistic electron flux dropouts in the outer radiation belt associated with corotating interaction regions

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Relativistic electron flux dropouts in the outer radiation b...

基于MRI影像组学的列线图对肾透明细胞癌病人同期远处转移的个体化预测

中国科学院地质与地球物理研究所2015年度（第15届）学术年会

作者： C.-j.yuan Q.-G.Zong W.-X.Wan H.Zhang A.-M.Du Institute of Geology and Geophysics Chinese Academy of Sciences Institute of Space Physics and Applied Technology Peking University

Understanding how the relativistic electron fluxes drop out in the outer radiation belt under different conditions is of great *** investigate which mechanisms may affect the dropouts under different solar wind conditions,1.5 – 6.0 Me V electron flux dropout events associated with 223 corotating interaction regions(CIRs) from 1994 to 2003 are studied using the observations of Solar,Anomalous,Magnetospheric Particle Explorer *** to the superposed epoch analysis,it is found that high solar wind dynamic pressure with the peak median value of about 7 n Pa is corresponding to the dropout of the median of the radiation belt content(RBC) index to 20% of the level before stream interface arrival,whereas low dynamic pressure with the peak median value of about 3 n Pa is related to the dropout of the median of RBC index to 40% of the level before stream interface ***,the influences of Russell-Mc Pherron effect with respect to interplanetary magnetic field orientation on dropouts are *** is pointed out that under positive Russell-Mc Pherron effect(+RM effect) condition,the median of RBC index can drop to 23% of the level before stream interface arrival,while for negative Russell-Mc Pherron effect(-RM effect) events,the median of RBC index only drops to 37% of the level before stream interface *** the evolution of phase space density profiles,the effect of +RM on dropouts can be through nonadiabatic loss.

关键词： It is common that CIRs are associated with electron flux dropouts It is suggested that high dynamic pressure leads to stronger dropout than low dynamic pressure Positive RM effect seems to result in more intense dropout than negative RM effect

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国际医学放射学杂志 2021年第2期44卷 247-248页

作者： X.Bai Q.B.Huang P.L.Zuo X.j.Zhang j.yuan X.Zhang 白旭

摘要目的评价基于多参数MRI影像组学的列线图对肾透明细胞癌(ccRCC)病人同步远处转移(SDM)的个体化预测效能。方法回顾性纳入2013年1月—2019年6月间收

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