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No relationship between biopsy sites near the main testicular vessels or rete testis and successful sperm retrieval using conventional or microdissection biopsies in 220 non-obstructive azoospermic men

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Asian journal of Andrology 2013年第6期15卷 795-798,I0009页

作者： j.ullrich schwarzer Heiko Steinfatt Heiko Steinfatt Manfred Schleyer Frank M Kōhn Klaus Fiedler2, Irene von Hertwig Gottfried Krüsmann Wolfgang Würfel Andrologie Centrum MOnchen MOnchen D-81241 Germany Kinderwunsch Centrum MOnchen MOnchen D-81241 Germany Andrologicum MOnchen MOnchen D-80331 Germany

In 220 consecutive patients with non-obstructive azoospermia, sperm retrieval was attempted by a combination of conventional and microdissection testicular sperm extraction （TESE）. For sperm retrieval, 2-3 conventional biopsies were performed followed by a microdissection TESE in cases of negative conventional biopsies. During the surgery, the vasculature of the testis was assessed using the operative microscope, and the location of positive biopsies was registered in relation to the blood supply. The overall sperm retrieval rate was 58.2%. From the initial conventional biopsies, sperm could be retrieved in 46.8% of the patients. With microdissection TESE, sperm could be retrieved from an additional 11.4% of the patients. The further use of microdissection TESE improved the sperm retrieval rate significantly （P=0.017）. No significant accumulation of positive biopsies was found towards the rete testis or the main testicular vessels.

关键词： intracytoplasmic sperm injection male infertility microdissection testicular sperm extraction non-obstructiveazoospermia sperm retrieval

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Multimodal imaging of experimental choroidal neovascularization

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International journal of Ophthalmology(English edition) 2022年第6期15卷 886-893页

作者： Ioanna Tsioti Xuan Liu Petra schwarzer Martin S.Zinkernagel Despina Kokona Department of Ophthalmology InselspitalBern University Hospitaland Department of BioMedical ResearchUniversity of BernBern 3010Switzerland Department of Ophthalmology the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’an 710061Shaanxi ProvinceChina

AIM:To compare choroidal neovascularization(CNV)lesion measurements obtained by in vivo imaging modalities,with whole mount histological preparations stained with isolectin GS-IB4,using a murine laser-induced CNV ***:B6 ***-Tg(Csf1 r-EGFP)1 Hume/j heterozygous adult mice were subjected to laser-induced CNV and were monitored by fluorescein angiography(FA),multicolor(MC)fundus imaging and optical coherence tomography angiography(OCTA)at day 14 after CNV *** pigment epithelium(RPE)whole mounts were prepared at the end of the experiment and were stained with isolectin *** areas were measured in all different imaging modalities at day 14 after CNV from three independent raters and were compared to choroidal-RPE whole *** correlation coefficient(ICC)type 2(2-way random model)and its 95%confidence intervals(CI)were calculated to measure the correlation between different raters’***’s rank correlation coefficient(Spearman’s r)was calculated for the comparison between FA,MC and OCTA data and histology ***:FA(early and late)and MC correlates well with the CNV measurements ex vivo with FA having slightly better correlation than MC(FA early Spearman’s r=0.7642,FA late Spearman’s r=0.7097,and MC Spearman’s r=0.7418),while the interobser ver reliability was good for both techniques(FA early ICC=0.976,FA late ICC=0.964,and MC ICC=0.846).In contrast,OCTA showed a poor correlation with ex vivo measurements(Spearman’s r=0.05716)and high variability between different raters(ICC=0.603).CONCLUSION:This study suggests that FA and MC imaging could be used for the evaluation of CNV areas in vivo while caution must be taken and comparison studies should be performed when OCTA is employed as a CNV monitoring tool in small rodents.

关键词： choroidal neovascularization in vivo imaging fluorescein angiography multicolor fundus imaging optical coherence tomography angiography

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Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

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Cellular & Molecular Immunology 2016年第2期13卷 251-262页

作者： Hana Kozakova Martin schwarzer Ludmila Tuckova Dagmar Srutkova Elzbieta Czarnowska Ilona Rosiak Tomas Hudcovic Irma Schabussova Petra Hermanova Zuzana Zakostelska Tamara Aleksandrzak-Piekarczyk Anna Koryszewska-Baginska Helena Tlaskalova-Hogenova Bozena Cukrowska Laboratory of Gnotobiology Institute of Microbiology Academy of Sciences of the Czech Republic Novy Hradek Czech Republic Department of Pathology the Children's Memorial Health Institute Warsaw Poland Institute of Specific Prophylaxis and Tropical Medicine Medical University of Vienna Vienna Austria Institute of Biochemistry and Biophysics Polish Academy of Sciences Warsaw Poland

Increasing numbers of clinical trials and animal experiments have shown that probiotic bacteria are promising tools for allergy prevention. Here, we analyzed the immunomodulatory properties of three selected lactobacillus strains and the impact of their mixture on allergic sensitization to Bet v I using a gnotobiotic mouse model. We showed that Lactobacillus （L.） rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 are recognized via Toll-like receptor 2 （TLR2） and nucleotide-binding oligomerization domain-containing protein 2 （NOD2） receptors and stimulate bone marrow-derived dendritic cells to produce cytokines in species- and strain-dependent manners. Colonization of germ-free （GF） mice with a mixture of all three strains （Lmix） improved the intestinal barrier by strengthening the apical junctional complexes of enterocytes and restoring the structures of microfilaments extending into the terminal web. Mice colonized with Lmix and sensitized to the Bet v I allergen showed significantly lower levels of allergen-specific IgE, IgG 1 and IgG2a and an elevated total IgA level in the sera and intestinal lavages as well as an increased transforming growth factor （TGF）-β level compared with the sensitized GF mice. Splenocytes and mesenteric lymph node cells from the Lmix-colonized mice showed the significant upregulation of TGF-β after in vitro stimulation with Bet v 1. Our results show that Lmix colonization improved the gut epithelial barrier and reduced allergic sensitization to Bet v 1. Furthermore, these findings were accompanied by the increased production of circulating and secretory IgA and the regulatory cytokine TGF-β. Thus, this mixture of three lactobacillus strains shows potential for use in the prevention of increased gut permeability and the onset of allergies in humans,

关键词： allergic sensitization germ-free intestinal barrier Lactobacillus probiotics

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改进零件几何形状减小热处理畸变

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热处理 2012年第3期27卷 62-66页

作者： C Andersch j schwarzer F Hoffmann H-W Zoch 顾剑锋 WT Stiftung Institutftur Werkstofftechnik 上海交通大学

因热处理时发生畸变,加之精度要求较高,零件热处理后通常都要进行机加工。由于通过机加工修正变形的成本太高,所以应尽可能减小零件的热处理畸变。本文研究结果证明,零件的热处理畸变与其几何形状有关。首先概述使零件几何形状成为畸变... 详细信息

因热处理时发生畸变,加之精度要求较高,零件热处理后通常都要进行机加工。由于通过机加工修正变形的成本太高,所以应尽可能减小零件的热处理畸变。本文研究结果证明,零件的热处理畸变与其几何形状有关。首先概述使零件几何形状成为畸变倾向重要载体的物理效应。其次,以复杂的汽车零件为例说明零件几何形状影响畸变的程度。最后介绍一种能更有效控制系统畸变的新途径。

关键词：热处理畸变几何形状

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