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Vascular units as advanced living materials for bottom-up engineering of perfusable 3D microvascular networks

Bioactive Materials 2024年第8期38卷 499-511页

作者： I.D.Orge H.Nogueira Pinto M.A.Silva S.j.Bidarra S.A.Ferreira I.Calejo r.Masereeuw S.M.Mihail C.C.barrias i3S-Instituto de Investigaçao e Inovaçao em Saúde Universidade do PortoPortoPortugal INEB-Instituto de Engenharia Biomedica Universidade do PortoPortoPortugal ICBAS-Instituto de Ciencias Biomedicas Abel Salazar Universidade do PortoPortoPortugal Division of Pharmacology Utrecht Institute for Pharmaceutical SciencesUtrecht UniversityUtrechtthe Netherlands

The timely establishment of functional neo-vasculature is pivotal for successful tissue development and regen-eration,remaining a central challenge in tissue *** this study,we present a novel(micro)vascular-ization strategy that explores the use of specialized“vascular units”(VUs)as building blocks to initiate blood vessel formation and create perfusable,stroma-embedded 3D microvascular networks from the *** demonstrate that VUs composed of endothelial progenitor cells and organ-specific fibroblasts exhibit high angiogenic potential when embedded in fibrin *** leads to the formation of VUs-derived capillaries,which fuse with adjacent capillaries to form stable microvascular beds within a supportive,extracellular matrix-rich fibroblastic *** a custom-designed biomimetic fibrin-based vessel-on-chip(VoC),we show that VUs-derived capillaries can inosculate with endothelialized microfluidic channels in the VoC and become ***,VUs can establish capillary bridges between channels,extending the microvascular network throughout the entire *** VUs and intestinal organoids(ios)are combined within the VoC,the VUs-derived capillaries and the intestinal fibroblasts progressively reach and envelop the *** promotes the formation of a supportive vascularized stroma around multiple ios in a single *** findings un-derscore the remarkable potential of VUs as building blocks for engineering microvascular networks,with ver-satile applications spanning from regenerative medicine to advanced in vitro models.

关键词： Engineered tissue Organ-on-chip Microtissue Spheroid endothelial colony-forming cells

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Surface engineering with ionic polymers on membranes for boron removal

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Frontiers of Chemical Science and Engineering 2024年第5期18卷 61-69页

作者： Xiting Zhang Chenyi Fang j Paul Chen Sui Zhang Department of Chemical and Biomolecular Engineering National University of SingaporeSingapore 117576Singapore Department of Civil and Environmental Engineering National University of SingaporeSingapore 119260Singapore College of Chemistry and Environmental Engineering Shenzhen UniversityShenzhen 518060China

removal of boric acid from seawater and wastewater using reverse osmosis membrane technologies is imperative and yet remains inadequately addressed by current commercial *** research efforts performed post-modification of reverse osmosis membranes to enhance boron rejection,which is usually accompanied by substantial sacrifice in water *** study delves into the surface engineering of low-pressure reverse osmosis membranes,aiming to elevate boron removal efficiency while maintaining optimal salt rejection and water *** were modified by the self-polymerization and co-deposition of dopamine and polystyrene sulfonate at varying ratios and *** surfaces became smoother and more hydrophilic after *** optimum membrane exhibited a water permeability of 9.2±0.1 L·m^(-2)·h^(-1)·bar^(-1),NaCl rejection of 95.8%±0.3%,and boron rejection of 49.7%±0.1% and 99.6%±0.3% at neutral and alkaline pH,*** water permeability is reduced by less than 15%,while the boron rejection is 3.7 times higher compared to the blank *** research provides a promising avenue for enhancing boron removal in reverse osmosis membranes and addressing water quality concerns in the desalination process.

关键词： membrane low-pressure reverse osmosis boron surface engineering

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Synthesis, relaxivity and Biodistribution of Novel Magnetic resonance Imaging (MrI) Contrast Agents:Polylysine (Gd-DTPA/DOTA) with Pendent Galactose Moieties as Hepatocyte-targeting Groups

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Chinese Chemical Letters 1997年第2期8卷 157-160页

作者： Zhuo, rX Fu, Yj Liao, j WUHAN UNIV DEPT CHEMWUHAN 430072PEOPLES R CHINA

DTPA or DOTA ligand was introduced into the pendent amino groups of polylysine ( 268) by mono-mixed anhydride of DTPA or DOTA,and then translated with Gd-EDTA. The resultant polymer chelates were further reacted with ... 详细信息

DTPA or DOTA ligand was introduced into the pendent amino groups of polylysine (r bar> 268) by mono-mixed anhydride of DTPA or DOTA,and then translated with Gd-EDTA. The resultant polymer chelates were further reacted with 6-O-bromoacetyl-D-galactose to give liver-specific contrast agents for magnetic resonance imaging. These new polymer conjugates were characterized by FT-Ir, ICP-AES, UV-Vis spectrophotometry and elemental analysis. Their in vitro spin-lattice relaxivity and body distribution in mice were investigated.

关键词： Gd-DTPA/DOTA with Pendent Galactose Moieties as Hepatocyte-targeting Groups Contrast Agents relaxivity and Biodistribution of Novel Magnetic resonance Imaging DTPA DOTA MrI

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Molecular Characterization of the Calvin Cycle Enzyme Phosphoribulokinase in the Stramenopile Alga Vaucheria litorea and the Plastid Hosting Mollusc Elysia chlorotica

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Molecular Plant 2009年第6期2卷 1384-1396页

作者： Mary E. rumpho Sirisha Pochareddy jared M. Worful Elizabeth j. Summer Debashish Bhattacharya Karen N. Pelletreau Mary S. Tyler jungho Lee james r. Manhart Kara M. Soule Department of Biochemistry Microbiology and Molecular Biology 5735 Hitchner Halt University of Maine Orono ME 04469 USA Department of Biology Texas A&M University College Station TX 77843 USA Department of Ecology Evolution and Natural Resources Institute of Marine and Coastal Sciences Rutgers University New Brunswick NJ 08901 USA School of Biology and Ecology University of Maine Orono ME 04469 USA Green Plant Institute #2-202 Bio Valley Seoul National University 103-2 Seodun Gwonseon Suwon Gyeonggi 441-853 Korea Department of Biochemistry and Biophysics Texas A&M University College Station TX 77843 USA

Phosphoribulokinase （PrK）, a nuclear-encoded plastid-localized enzyme unique to the photosynthetic carbon reduction （Calvin） cycle, was cloned and characterized from the stramenopile alga Vaucheria litorea. This alga is the source of plastids for the mollusc （sea slug） Elysia chlorotica which enable the animal to survive for months solely by photoautotrophic CO2 fixation. The 1633-bp V. litorea prk gene was cloned and the coding region, found to be interrupted by four introns, encodes a 405-amino acid protein. This protein contains the typical bipartite target sequence expected of nuclearencoded proteins that are directed to complex （i.e. four membrane-bound） algal plastids. De novo synthesis of PrK and enzyme activity were detected in E. chlorotica in spite of having been starved of V. litorea for several months. Unlike the algal enzyme, PrK in the sea slug did not exhibit redox regulation. Two copies of partial PrK-encoding genes were isolated from both sea slug and aposymbiotic sea slug egg DNA using PCr. Each copy contains the nucleotide region spanning exon 1 and part of exon 2 of V litorea prk, including the bipartite targeting peptide. However, the larger prk fragment also includes intron 1. The exon and intron sequences of prk in E. chlorotica and V/itorea are nearly identical. These data suggest that PrK is differentially regulated in V. litorea and E. chlorotica and at least a portion of the V. litorea nuclear PrK gene is present in sea slugs that have been starved for several months.

关键词： Alga Calvin cycle Elysia chlorotica kleptoplast mollusc phosphoribulokinase photosynthesis plastid redox regulation stramenopile symbiosis Vaucheria litorea.

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THE -PrOBLEM FOr HOLOMOrPHIC (0,2)-FOrMS ON PSEUDOCONVEX DOMAINS IN SEPArABLE HILBErT SPACES AND D.F.N. SPACES

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Chinese Annals of Mathematics,Series B 2002年第1期23卷 67-74页

作者： j.LEE K.H.SHON DepartmentofMathematicsEducation AndongNationalUniversityAndong760-749Korea DepartmentofMathematics PusanNationalUniversityPusan609-735Korea

This paper shows that the 8-problem for holomorphic (0, 2)-forms on Hubert spaces is solv-able on pseudoconvex open subsets. By using this result, the authors investigate the existence of the solution of the -equation... 详细信息

关键词： -prob1em Pseudoconvex domain Nuclear operator D.F.N. space

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H1N1流感早期胸部X线表现对预后评估意义的相关研究

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国际医学放射学杂志 2010年第3期 286-287页

作者：沈晶 G. Aciram A. bar-Shai j. Sosna O. rogowski G. rosen I. Weinstein Departments of radiology Internal Medicine 'D' and Pulmonology Tel Aviv University

摘要目的回顾性研究A型流感(H1NI)病人早期胸部X线表现对临床预后评估的意义。材料与方法此项研究通过伦理委员会的

关键词：胸部躯干线表现 H1N1 流感流行性感冒预后评估

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马普街5号住宅大楼——以色列特拉维夫-雅法

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世界建筑导报 2023年第3期38卷 84-87页

作者： Gidi bar Orian Kfir Peled Nir Ovadia Noam Elkabetz Amit Geron(摄影) bar Orian建筑师事务所

马普街5号是一栋方形的新建住宅大楼,位于特拉维夫白城中心马普街与Yehoash街道的拐角处--白城被联合国教科文组织列入世界遗产名录,因该地大量的包豪斯(Bauhaus)和折中主义建筑风格的建筑物而享誉世界。该建筑位于特拉维夫最理想的地... 详细信息

马普街5号是一栋方形的新建住宅大楼,位于特拉维夫白城中心马普街与Yehoash街道的拐角处--白城被联合国教科文组织列入世界遗产名录,因该地大量的包豪斯(Bauhaus)和折中主义建筑风格的建筑物而享誉世界。该建筑位于特拉维夫最理想的地区之一,其地块在大小和形状上(1.3德南(dunam:以色列单位))都异于寻常,是当地普通地块的三倍大,与特拉维夫地中海海滨大道相距约200米(600英尺)--正是在这样一个被忽视的空地上,bar Orian接受了委托,要在此处构思和规划建设一栋新建筑。这片弃用的地块反而为其提供了一个极佳的机会,得以展示一栋向特拉维夫中东-地中海构造风格致敬的建筑,同时创造了一种新的当代视觉呈现。

关键词：建筑风格住宅大楼世界遗产名录海滨大道包豪斯构造风格联合国教科文组织马普

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A NOVEL METHOD FOr EVALUATING PLANE STrESS DYNAMIC FrACTUrE TOUGHNESS OF 0Cr18Ni10Ti STAINLESS STEEL WELDED jOINTS

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Acta Metallurgica Sinica(English Letters) 2008年第4期21卷 303-312页

作者： Z.j. Xu Y.L. Li School of Aeronautics Northwestern Polytechnical University Xi＇an 710072 China

A novel method was proposed for the evaluation of Mode I dynamic fracture toughness （DFT） under plane stress and small scale yielding conditions for welded joints of stainless steel （SS）, 0Cr18Ni10Ti. In a hybrid experimental-numerical approach, the experiments were carried out on the Hopkinson pressure bar apparatus, and three dimensional （3D） transient numerical simulations were performed by a finite element （FE） computer program. Macroscopical plastic deformation was observed at the loading and supporting points, on the specimens, after the test, which could cause a large error if omitted in the numerical simulation. Therefore, elustic-viscoplustic analysis was performed on the specimen by adopting the johnson-Cook （j-C） model to describe the rate-dependent plastic flow behavior of the material. The material heterogeneity in the mismatched welded joints, induced by the difference in the base metal （BM） and the weld metal （WM） in yield stress, has also been taken into consideration by using the j-C models separately. Good accordance was obtained between the experimental and the computational results by the present approach. The relationship between plane stress DFT and loading rate was also obtained on the order of 108 MPa.m^1/2.s^-1.

关键词： 0Cr18Ni10Ti Welded joint High strain rate Dynamic fracture Plane stress

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Shark scavenging behavior in the presence of competition

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Current Zoology 2010年第1期56卷 100-108页

作者： Shannon P.GErrY Andrea j.SCOTT Department of Biological Sciences University of Rhode Island Department of Biological Sciences Wellesley College

The distribution of organisms within a community can often be determined by the degree of plasticity or degree of specialization of resource acquisition. resource acquisition is often based on the morphology of an organism, behavior, or a combination of both. Performance tests of feeding can identify the possible interactions that allow one species to better exploit a prey item. Scavenging behaviors in the presence or absence of a competitor were investigated by quantifying prey selection in a trophic generalist, spiny dogfish Squalus acanthias, and atrophic specialist, smooth-hounds Mustelus canis, in order to determine if each shark scavenged according to its jaw morphology. The diet of dogfish consists of small fishes, squid, ctenophores, and bi- valves; they are expected to be nonselective predators. Smooth-hounds primarily feed on crustaceans; therefore, they are predicted to select crabs over other prey types. Prey selection was quantified by ranking each prey item according to the order it was consumed. Dietary shifts were analyzed by comparing the percentage of each prey item selected during solitary versus competitive scavenging. When scavenging alone, dogfish prefer herring and squid, which are easily handled by the cutting dentition of dogfish. Dogfish shift their diet to include a greater number of prey types when scavenging with a competitor. Smooth-hounds scavenge on squid, herring, and shrimp when alone, but increase the number of crabs in the diet when scavenging competitively. Competition causes smooth-hounds to scavenge according to their jaw morphology and locomotor abilities, which enables them to feed on a specialized resource [Current Zoology 56 （1）： 100-108 2010].

关键词： Optimal foraging theory Prey selection Squalus acanthias Mustelus canis

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The effects of sun exposure on carotenoid accumulation and oxidative stress in the retina of the House Finch(Haemorhous mexicanus)

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Chinese Birds 2016年第1期7卷 56-67页

作者： Matthew B.Toomey Kevin j.McGraw School of Life Sciences Arizona State University Department of Pathology and Immunology Washington University Medical School

Background: Diet-derived carotenoid pigments are concentrated in the retinas of birds and serve a variety of functions, including photoprotection. In domesticated bird species(e.g., chickens and quail), retinal carotenoid pigmentation has been shown to respond to large manipulations in light exposure and provide protection against photodamage. However, it is not known if or how wild birds respond to ecologically relevant variation in sun ***: We manipulated the duration of natural sunlight exposure and dietary carotenoid levels in wild-caught captive House Finches(Haemorhous mexicanus), then measured carotenoid accumulation and oxidative stress in the ***: We found no significant effects of sun exposure on retinal levels of carotenoids or lipid peroxidation, in replicate experiments, in winter(jan–Mar) and spring/summer(May–june). Dietary carotenoid supplementation in the spring/summer experiment led to significantly higher retinal carotenoid levels, but did not affect lipid peroxidation. Carotenoid levels differed significantly between the winter and spring/summer experiments, with higher retinal and lower plasma carotenoid levels in birds from the later ***: Our results suggest that variation in the duration of exposure to direct sunlight have limited influence on intraspecific variation in retinal carotenoid accumulation, but that accumulation may track other seasonal–environmental cues and physiological processes.

关键词： Carotenoids Haemorhous mexicanus House Finch Photostress Vision

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