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Preprotein Import into Chloroplasts via the Toc and Tic Complexes Is Regulated by Redox Signals in Pisum sativum

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Molecular Plant 2009年第6期2卷 1181-1197页

作者： Anna Stengel j. philipp b.nz bob b. buchanan j.rgen Soil bettina bolter Munich Center for Integrated Protein Science CiPS^M Ludwig-Maximilians-Universitat Munchen Feodor-Lynen-Strasse 25 D-81377 Munich Germany Department of biology I Botany Ludwig-Maximilians-Universitat Munchen Groβhaderner Strasse 2-4 D-82152 Planegg-Martinsried Germany Department of Plant and Microbial biology University of California 411 Koshland Hall Berkeley CA 94720 USA

The import of nuclear-encoded preproteins is necessary to maintain chloroplast function. The recognition and transfer of most precursor proteins across the chloroplast envelopes are facilitated by two membrane-inserted protein complexes, the translocons of the chloroplast outer and inner envelope （Toc and Tic complexes, respectively）. Several signals have been invoked to regulate the import of preproteins. In our study, we were interested in redox-based import regulation mediated by two signals： regulation based on thiols and on the metabolic NADP＋/NADPH ratio. We sought to identify the proteins participating in the regulation of these transport pathways and to characterize the preprotein subgroups whose import is redox-dependent. Our results provide evidence that the formation and reduction of disulfide bridges in the Toc receptors and Toc translocation channel have a strong influence on import yield of all tested preproteins that depend on the Toc complex for translocation. Furthermore, the metabolic NADP＋/NADPH ratio influences not only the composition of the Tic complex, but also the import efficiency of most, but not all, preproteins tested. Thus, several Tic subcomplexes appear to participate in the translocation of different preprotein subgroups, and the redox-active compo- nents of these complexes likely play a role in regulating transport.

关键词： Metabolic regulation molecular transport transport processes/kinetics chloroplast biology protein targeting.

Construction of 2D/2D Ti_(3)C_(2)T_(x)MXene/CdS heteroj.nction with photothermal effect for efficient photocatalytic hydrogen production

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j.urnal of Materials Science & Technology 2024年第4期171卷 1-9页

作者： T.Y.Huang Z.Yang S.Y.Yang Z.H.Dai Y.j.Liu j.H.Liao G.Y.Zhong Z.j.Xie Y.P.Fang S.S.Zhang Key Laboratory for biobased Materials and Energy of Ministry of Education Guangdong Laboratory for Lingnan Modern AgricultureCollege of Materials and EnergySouth China Agricultural UniversityGuangzhou 510643China School of Physics and Optoelectronics South China University of TechnologyGuangzhou 510640China School of Chemical Engineering and Energy Technology Guangdong Provincial Key Laboratory of Distributed Energy SystemsDongguan University of TechnologyDongguan 523808China

The insensitivity of semiconductors to visible and infrared light is a key constraint on the utilization of light energy in photocatalytic *** photocatalysts with full-spectrum absorption through surface engineering is an effective approach to fully harnessing light energy in semiconductor ***,a novel stable Ti_(3)C_(2)T_(x)MXene/CdS heteroj.nction catalyst is obtained by in-situ epitaxial growth of two-dimensional(2D)CdS nanosheets on 2D MXene interface via a solvothermal *** exceptional light absorption properties of MXene confer outstanding full-spectrum driven photocat-alytic hydrogen evolution capability upon the heterogeneous *** unique 2D/2D structure effectively mitigated the recombination of photogenerated carriers,enhancing the photocatalytic performance of the ***,the composite catalyst exhibits a significantly higher surface temperature of 80.4℃under visible light irradiation at an intensity of 0.1 W/cm^(2),which is 1.84 times higher than that of *** irradiation of visible and near infrared light,the composite catalyst with photothermal ef-fect demonstrates a remarkable hydrogen evolution rate of 65.4 mmol g^(-1)h^(-1),which is 7.2 times higher than that of CdS *** study introduces a novel approach for constructing full-spectrum absorption catalysts and expands the application of the photothermal effect in photocatalytic hydrogen evolution research.

关键词： Two-dimensional structure Heterostructure Photothermal effect Photocatalyst Hydrogen evolution

Mg-based materials diminish tumor spreading and cancer metastases

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bioactive Materials 2023年第1期19卷 594-610页

作者： philipp Globig Roshani Madurawala Regine Willumeit-Römer Fernanda Martini Elisa Mazzoni bérengère j.C.Luthringer-Feyerabend Institute of Metallic biomaterials Helmholtz-Zentrum Hereon GmbH21502GeesthachtGermany Department of Medical Sciences University of Ferrara44121FerraraItaly

Cancer metastases are the most common causes of cancer-related *** formation of secondary tumors at different sites in the human body can impair multiple organ function and dramatically decrease the survival of the *** this stage,it is difficulty to treat tumor growth and spreading due to arising therapy ***,it is important to prevent cancer metastases and to increase subsequent cancer therapy *** metastases are conventionally treated with radiation or ***,these treatments elicit lots of side effects,wherefore novel local treatment approaches are currently *** studies already showed anticancer activity of specially designed degradable magnesium(Mg)alloys by reducing the cancer cell *** this work,we investigated the impact of these Mg-based materials on different steps of the metastatic cascade including cancer cell migration,invasion,and cancer-induced ***,Mg and Mg-6Ag reduced cell migration and invasion of osteosarcoma cells in coculture with ***,the Mg-based materials used in this study diminished the cancer-induced *** cells incubated with conditioned media obtained from these Mg and Mg-6Ag showed a reduced cell layer permeability,a reduced proliferation and inhibited cell *** tube formation as a last step of angiogenesis was stimulated with the presence of Mg under normoxia and diminished under hypoxia.

关键词： Cancer Osteosarcoma Magnesium degradation Cell migration Cell invasion Angiogenesis

Electrospinning as a route to advanced carbon fibre materials for selected low-temperature electrochemical devices:A review

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j.urnal of Energy Chemistry 2021年第8期30卷 492-529,I0011页

作者： Yue Wen Matt D.R.Kok j.rge Pavel Victoria Tafoya Ana bj.rge Sobrido Ellsworth bell j.ff T.Gostick Servann Herou philipp Schlee Maria-Magdalena Titirici Dan j.L.brett Paul R.Shearing Rhodri j.rvis The Electrochemical Innovation Lab Dept.of Chemical EngineeringUniversity College LondonTorrington PlaceWC1E 7JE LondonUK Queen Mary University of London School of Engineering and Materials ScienceMile End RoadE14NS LondonUK Department of Chemical Engineering University of WaterlooWaterlooCanada Department of Chemical Engineering Imperial College LondonSouth Kensington CampusSW72AZ LondonUK

Electrospinning has been proven as a highly versatile fabrication method for producing nano-structured fibres with controllable morphology,of both the fibres themselves and the void structure of the ***,it is possible to use heteroatom doped polymers or to include catalytic precursors in the electrospinning solution to control the surface properties of the *** factors make it an ideal method for the production of electrodes and flow media for a variety of electrochemical devices,enabling reduction in mass transport and activation overpotentials and therefore increasing ***,the use of biomass as a polymer source has recently gained attention for the ability to embed sustainable principles in the materials of electrochemical devices,complementing their ability to allow an increase in the use of renewable electricity via their *** this review,the historical and recent developments of electrospun materials for application in redox flow batteries,fuel cells,metal air batteries and supercapacitors are thoroughly reviewed,including an overview of the electrospinning process and a guide to best ***,we provide an outlook for the emerging use of this process in the field of electrochemical energy devices with the hope that the combination of tailored microstructure,surface functionality and computer modelling will herald a new era of bespoke functional materials that can significantly improve the performance of the devices in which they are used.

关键词： Electrospinning Redox flow batteries Fuel cells Metal-air batteries Supercapacitors

Loss of Notch signaling in skeletal stem cells enhances bone formation with aging

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bone Research 2023年第4期11卷 737-750页

作者： Lindsey H.Remark Kevin Leclerc Malissa Ramsukh Ziyan Lin Sooyeon Lee backialakshmi Dharmalingam Lauren Gillinov Vasudev V.Nayak Paulo El Parente Margaux Sambon Pablo j.Atria Mohamed A.E.Ali Lukasz Witek Alesha b.Castillo Christopher Y.Park Ralf H.Adams Aristotelis Tsirigos Sophie M.Morgani philipp Leucht Department of Orthopaedic Surgery NYU Robert I.Grossman School of MedicineNew YorkNYUSA Applied bioinformatics Laboratories NYU Grossman School of MedicineNew YorkNYUSA Institute of Comparative Molecular Endocrinology Ulm UniversityUlmGermany Max Planck Institute for Molecular biomedicine Department of Tissue Morphogenesisand University of MünsterFaculty of MedicineD-48149 MünsterGermany Department of biochemistry and Molecular biology University of Miami Miller School of MedicineMiamiFLUSA Department of Pathology NYU Robert I.Grossman School of MedicineNew YorkNYUSA biomaterials Division New York University College of DentistryNew YorkNYUSA Hansj.rg Wyss Department of Plastic Surgery NYU Grossman School of MedicineNew York UniversityNew YorkNYUSA Department of biomedical Engineering Tandon School of EngineeringNew York UniversityBrooklynNYUSA Department of Cell biology NYU Robert I.Grossman School of MedicineNew YorkNYUSA

Skeletal stem and progenitor cells(SSPCs) perform bone maintenance and repair. With age, they produce fewer osteoblasts and more adipocytes leading to a loss of skeletal integrity. The molecular mechanisms that underlie this detrimental transformation are largely unknown. Single-cell RNA sequencing revealed that Notch signaling becomes elevated in SSPCs during aging. To examine the role of increased Notch activity, we deleted Nicastrin, an essential Notch pathway component, in SSPCs in vivo. Middle-aged conditional knockout mice displayed elevated SSPC osteo-lineage gene expression, increased trabecular bone mass, reduced bone marrow adiposity, and enhanced bone repair. Thus, Notch regulates SSPC cell fate decisions, and moderating Notch signaling ameliorates the skeletal aging phenotype, increasing bone mass even beyond that of young mice. Finally, we identified the transcription factor Ebf3 as a downstream mediator of Notch signaling in SSPCs that is dysregulated with aging, highlighting it as a promising therapeutic target to rej.venate the aged skeleton.

关键词： Notch skeletal elevated

3D printing of multilayered scaffolds for rotator cuff tendon regeneration

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bioactive Materials 2020年第3期5卷 636-643页

作者： Xiping j.ang Shaohua Wu Mitchell Kuss Yunfan Kong Wen Shi philipp N.Streubel Tieshi Li bin Duan Mary&Dick Holland Regenerative Medicine Program Division of CardiologyDepartment of Internal MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Molecular Genetics and Cell biology Program Department of GeneticsCell Biology and AnatomyUniversity of Nebraska Medical CenterOmahaNE68198USA College of Textiles&Clothing Collaborative Innovation Center of Marine Biomass FibersQingdao UniversityQingdao266071China Department of Orthopedic Surgery and Rehabilitation University of Nebraska Medical CenterOmahaNE68198USA Department of Pediatrics University of Nebraska Medical CenterOmahaNE68198USA Department of Surgery College of MedicineUniversity of Nebraska Medical CenterOmahaNE68198USA Department of Mechanical and Materials Engineering University of Nebraska-LincolnLincolnNE68516USA

Repairing massive rotator cuff tendon defects remains a challenge due to the high retear rate after surgical intervention.3D printing has emerged as a promising technique that enables the fabrication of engineered tissues with heterogeneous structures and mechanical properties,as well as controllable microenvironments for tendon *** this study,we developed a new strategy for rotator cuff tendon repair by combining a 3D printed scaffold of polylactic-co-glycolic acid(PLGA)with cell-laden collagen-fibrin *** designed and fabricated two types of scaffolds:one featuring a separate layer-by-layer structure and another with a tri-layered structure as a *** tensile tests showed that both types of scaffolds had improved mechanical properties compared to single-layered PLGA *** printed scaffold with collagen-fibrin hydrogels effectively supported the growth,proliferation,and tenogenic differentiation of human adipose-derived mesenchymal stem *** implantation of the multilayered scaffolds demonstrated their excellent in vivo *** study demonstrates the feasibility of 3D printing multilayered scaffolds for application in rotator cuff tendon regeneration.

关键词： Collagen-fibrin hydrogel Tenogenic differentiation Tendon repair Tissue engineering

A New β-Estradiol-lnducible Vector Set that Facilitates Easy Construction and Efficient Expression of Transgenes Reveals CbL3-Dependent Cytoplasm to Tonoplast Translocation of CIPK5

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Molecular Plant 2013年第6期6卷 1814-1829页

作者： Kathrin Schliicking Kai H. Edel Maria M. Drerupa philipp Koster Christian Eckert Leonie Steinhorst Rainer Waadt Oliver batistic j.rg Kudla Institut for biologie und biotechnologie der Pflanzen Universit~t MQnster Schlossplatz 4 48149 MQnster Gel'many _ . _ Present address: University of California San Diego Division of Biological Sciences Cell and Developmental Biology Section 9500 Gilman Drive #0116 LaJolla CA 92093-0116 USA

Transient and stable expression of transgenes is central to many investigations in plant biology research. Chemical regulation of expression can circumvent problems of plant lethality caused by constitutive overexpression or allow inducible knock （out/down） approaches. Several chemically inducible or repressible systems have been described and successfully applied. However, cloning and application-specific modification of most available inducible expression systems have been limited and remained complicated due to restricted cloning options. Here we describe a new set of 57 vectors that enable transgene expression in transiently or stably transformed cells. All vectors harbor a synthetically optimized XVE expression cassette, allowing I^-estradiol mediated protein expression. Plasmids are equipped with the reporter genes GUS, GFP, mCherry, or with HA and Strepll epitope tags and harbor an optimized multiple cloning site for flexible and simple clon- ing strategies. Moreover, the vector design allows simple substitution of the driving promoter to achieve tissue-specificity or to modulate expression ranges of inducible transgene expression. We report details of the kinetics and dose-dependence of expression induction in Arabidopsis leaf mesophyll protoplasts, transiently transformed Nicotiana benthamiana leaves, and stably transformed Arabidopsis plants. Using these vectors, we investigated the influence of CbL （Calcineurin b-like） protein expression on the subcellular localization of CIPKs （Calcineurin b-like interacting protein kinases）. These analyses uncovered that induced co-expression of CbL3 is fully sufficient for dynamic translocation of CIPK5 from the cytoplasm to the tonoplast. Thus, the vector system presented here facilitates a broad range of research applications.

关键词： vector inducible gene expression βestradiol Arabidopsis CbL CIPK.

A novel diagnostic gene region for distinguishing between two pest fruit flies:bactrocera tryoni(Froggatt)and bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

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Insect Science 2024年第4期31卷 1285-1295页

作者： Melissa L.Starkie Elizabeth V.Fowler Alexander M.Piper Xiaocheng Zhu Pauline Wyatt David Gopurenko Matt N.Krosch Francesca Strutt Karen F.Armstrong Hamish Patrick Mark K.Schutze Mark j.blacket Department of Agriculture and Fisheries BiosecurityBrisbaneQueenslandAustralia Agriculture Victoria AgriBioBundooraVictoriaAustralia Department of Primary Industries Wagga Wagga Agricultural InstituteWagga WaggaNew South WalesAustralia Forensic Services Group Queensland Police ServiceBrisbaneQueenslandAustralia Department of Pest-management and Conservation Lincoln UniversityLincolnNew Zealand better border biosecurity(b3 B3nz.org.nz)LincolnNew Zealand

bactrocera tryoni and bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host *** need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference *** morphologically similar,adults may be separated based on subtle characters;however,some characters exhibit intraspecific variability,creating overlap between the two ***,there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between *** and ***;therefore,ambiguous samples remain *** we report the first molecular marker that can consistently distinguish between *** and *** diagnostic region consists of two adj.cent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene *** confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation system(ARMS)PCR assay for rapid diagnosis of the *** assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adj.cent SNPs(AA)present in *** and the other targeting adj.cent SNPs present in ***(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly ***,this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses.

关键词： ARMS-PCR colony molecular diagnostics pangolin tetra-primer

Diagnostics for a troubled backbone:testing topological hypotheses of trapelioid lichenized fungi in a large-scale phylogeny of Ostropomycetidae(Lecanoromycetes)

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Fungal Diversity 2015年第4期73卷 239-258页

作者： philipp Resl Kevin Schneider Martin Westberg Christian Printzen Zdeněk Palice Göran Thor Alan Fryday Helmut Mayrhofer Toby Spribille Institute of Plant Sciences NAWI GrazUniversity of GrazHolteigasse 6A-8010 GrazAustria Department of botany Swedish Museum of Natural HistoryP.O.Box 50007SE-10405 StockholmSweden Senckenberg Forschungsinstitut und Naturmuseum Senckenberganlage 25D-60325 Frankfurt am MainGermany Institute of botany Academy of Sciences of the Czech RepublicZámek 125243 PrůhoniceCzech Republic Department of botany Faculty of SciencesCharles University in PragueBenátská212801 Praha 2Czech Republic Department of Ecology Swedish University of Agricultural SciencesP.O.Box 7044SE-75007 UppsalaSweden Herbarium Department of Plant BiologyMichigan State UniversityEast LansingMI 48824USA Division of biological Sciences University of Montana32 Campus DriveMissoulaMT 59812USA

Trapelioid fungi constitute a widespread group of mostly crust-forming lichen mycobionts that are key to understanding the early evolutionary splits in the Ostropomycetidae,the second-most species-rich subclass of lichenized *** uncertain phylogenetic resolution of the approximately 170 species referred to this group contributes to a poorly resolved backbone for the entire *** on a data set including 657 newly generated sequences from four ribosomal and four protein-coding gene loci,we tested a series of a priori and new evolutionary hypotheses regarding the relationships of trapelioid clades within *** found strong support for a monophyletic group of nine core trapelioid genera but no statistical support to rej.ct the long-standing hypothesis that trapelioid genera are sister to baeomycetaceae or ***,we can rej.ct a sister group relationship to Ostropales with high *** data also shed light on several longstanding questions,recovering Anamylopsoraceae nested within baeomycetaceae,elucidating two maj.r monophyletic groups within trapelioids(recognized here as Trapeliaceae and Xylographaceae),and rej.cting the monophyly of the genus *** transfer eleven species of the latter genus to Lambiella and describe the genus Parainoa to accommodate a previously misunderstood species of *** phylogenetic studies in Ostropomycetidae have invoked bdivergence order^for drawing taxonomic conclusions on higher level *** data show that if backbone support is lacking,contrasting solutions may be recovered with different or added *** accordingly urge caution in concluding evolutionary relationships from unresolved phylogenies.

关键词： Ascomycota Fungi Lambiella Lecanoromycetes Ostropomycetidae Parainoa Paraphyly SOWH test Taxon sampling