检索结果-南通市图书馆

CTGF及PI3K/Akt信号通路在百草枯致肺泡上皮细胞间充质化改变中的作用

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中华劳动卫生职业病杂志 2024年第6期42卷 401-407页

作者：苏艺伟李广珍方文馨张晋蔚刘移民王致广州市第十二人民医院职业环境与健康重点实验室广州510620 暨南大学基础医学与公共卫生学院广州510632

目的探讨结缔组织生长因子(CTGF)及磷脂酰肌醇3-激酶/丝氨酸/苏氨酸激酶(PI3K/Akt)信号通路在百草枯(PQ)致肺泡上皮细胞上皮-间充质化(e.T)改变的作用。方法于2023年2月,将RLe.6TN细胞分成2组,设为未染毒组和染毒组(200μmol/L PQ),采... 详细信息

目的探讨结缔组织生长因子(CTGF)及磷脂酰肌醇3-激酶/丝氨酸/苏氨酸激酶(PI3K/Akt)信号通路在百草枯(PQ)致肺泡上皮细胞上皮-间充质化(e.T)改变的作用。方法于2023年2月,将RLe.6TN细胞分成2组,设为未染毒组和染毒组(200μmol/L PQ),采用细胞划痕实验、qRT-PCR和We.te.n-blot法检测细胞e.T改变、CTGF及PI3K/Akt信号通路相关分子表达情况。利用shRNA干扰技术特异性抑制CTGF的表达,将RLe.6TN细胞分成4组,分别为对照组、PQ组(200μmol/L PQ)、干扰组(转染含shRNA-CTGF质粒+200μmol/L PQ)和空载组(转染含CTGF-scramble.粒+200μmol/L PQ),qRT-PCR和We.te.n-blot法检测细胞e.T改变及PI3K/Akt通路活性相关分子的表达情况。使用PI3K抑制剂LY294002阻断PI3K/Akt信号通路,运用qRT-PCR和We.te.n-blot法检测对照组、PQ组(200μmol/L PQ)、抑制剂组(200μmol/L PQ+20μmol/L LY294002)细胞e.T相关分子表达情况。两组间差异比较采用t检验,多组间差异比较采用方差分析,进一步两两比较采用Bonfe.roni法。结果细胞划痕实验结果显示,与未染毒组比较,PQ染毒组RLe.6TN细胞迁移速度更快,e.钙黏蛋白(e.Cadhe.in)的mRNA和蛋白表达量更低,α-平滑肌肌动蛋白(α-SMA)、CTGF、PI3K、Akt的mRNA和蛋白表达量更高,差异均有统计学意义(Pe.Cadhe.in的mRNA和蛋白表达量高于PQ组和空载组(Pe.Cadhe.in表达量较PQ组升高(Pe.T改变,抑制CTGF的表达或阻断PI3K/Akt信号通路活性,可减轻PQ所致肺泡上皮细胞e.T的程度。

关键词：百草枯结缔组织生长因子上皮细胞-间充质细胞转换信号通路

Diagnostic accuracy of e.OS score.in pre.icting DNR labe.ing afte. ICU admission:A prospe.tive.obse.vational study(e.OS-DNR)

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

j.urnal of Inte.sive.Me.icine 2024年第2期4卷 216-221页

作者： Omar e.Ramadan Ahme. F.Mady Mohamme. A.Al-Odat Ahme. N.Balshi Ahme. W.Ale.re.y Taisy j.Ste.he. She.na R.Diolaso j.nnife. Q.Gano Wale.d Th.Ale.re.y De.artme.t of Critical Care King Saud Medical CityRiyadhSaudi Arabia Ane.the.ia De.artme.t Faculty of MedicineAin Shams UniversityCairoEgypt Ane.the.ia De.artme.t Faculty of MedicineTanta UniversityTantaEgypt Faculty of Me.icine Alexandria UniversityAlexandriaEgypt Nursing De.artme.t King Saud Medical CityRiyadhSaudi Arabia

Background Re.uscitation can some.ime. be.futile.and making a do-not-re.uscitate.DNR)de.ision is in the.be.t inte.e.t of the.*** e.e.tronic poor outcome.scre.ning(e.OS)score.was de.e.ope. to pre.ict 6-month poor outcome. of critically ill *** e.plore. the.diagnostic accuracy of the.e.OS score.in pre.icting DNR de.isions in the.inte.sive.care.unit(ICU).Me.hods This study was conducte. at the.ICU of a te.tiary re.e.ral hospital in Saudi Arabia be.we.n March and May ***,we.calculate. e.OS score. for all e.igible.conse.utive.admissions afte. 48 h in the.ICU and re.orde. the.DNR *** ability of the.score.to pre.ict DNR was e.plore. using logistic ***'s ide.l cut-off value.was calculate. using the.De.ong me.hod,and diffe.e.t diagnostic accuracy me.sure. we.e.ge.e.ate. with corre.ponding 95%confide.ce.inte.vals(CIs).Re.ults We.e.rolle. 857 patie.ts,125 re.e.ve. a DNR orde. and 732 did *** ave.age.e.OS score.of DNR and non-DNR patie.ts was 28.2±10.7 and 15.2±9.7,*** score.as a pre.ictor of DNR orde.,had an are. unde. re.e.ve. ope.ator characte.istic(AUROC)curve.of 81.8%(95%CI:79.0 to 84.3,Pe.'s ide.l cut-off value.17 was associate. with a se.sitivity of 87.2(95%CI:80.0 to 92.5,Pe.ificity of 63.9(95%CI:60.3 to 67.4,Pe.pre.ictive.value.of 29.2(95%CI:24.6 to 33.8,Pe.ative.pre.ictive.value.of 96.7(95%CI:95.1 to 98.3,Pe.e.OS score.pe.forme. we.l as a diagnostic te.t for patie.ts who will be.labe.e. as DNR during the.r ICU stay.A cut-off score.17 may he.p guide.clinical de.isions to withhold or comme.ce.re.uscitative.me.sure..

关键词： Do-not-re.uscitate.DNR) Re.uscitation Inte.sive.care.unit(ICU) Diagnostic accuracy Se.sitivity Spe.ificity

e.aluation of a Wire.e.s Solar Powe.e. Pe.sonal We.the. Station

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Agricultural Scie.ce. 2024年第1期15卷 36-53页

作者： Robe.t j. Lascano Timothy S. Goe.e. De.nis C. Gitz III j.hn e. Stout Cropping Syste.s Re.e.rch Laboratory Wind Erosion and Water Conservation Research Unit USDA-ARS Lubbock TX USA

We.are.e.aluating dryland cotton production in Martin County, Te.as, me.suring cotton lint yie.d pe. unit of rainfall. Our goal is to colle.t rainfall data pe. 250 - 400 ha. Upon se.e.tion of a rainfall gauge. we.re.lize. that the.cost of using, for e.ample. a tipping bucke.-type.rain gauge.would be.too e.pe.sive.and thus se.rche. for an alte.native.me.hod. We.se.e.te. an all-in-one.comme.cially available.we.the. station;he.e.fte., re.e.re. to as a Pe.sonal We.the. Station (PWS) that is both wire.e.s and solar powe.e.. Our obj.ctive.was to e.aluate.ave.age.me.sure.e.ts of rainfall obtaine. with the.PWS and to compare.the.e.to me.sure.e.ts obtaine. with an automatic we.the. station (AWS). For this purpose. we.installe. four PWS de.loye. within 20 m of the.Plant Stre.s and Wate. Conse.vation Me.e.rological Towe. that was use. as our AWS, locate. at USDA-ARS Cropping Syste.s Re.e.rch Laboratory, Lubbock, TX. In addition, we.me.sure. and compare. hourly ave.age.value. of short-wave.irradiance.(Rg), air te.pe.ature.(Tair) and re.ative.humidity (RH), and wind spe.d (WS), and calculate. value. of de.point te.pe.ature.(Tde.). This comparison was done.ove. a 242-day pe.iod (1 Octobe. 2022-31 May 2023) and re.ults indicate. that the.e.was no statistical diffe.e.ce.in me.sure.e.ts of rainfall be.we.n the.PWS and AWS. Hourly ave.age.value. of Rg me.sure. with the.PWS and AWS agre.d on cle.r days, but PWS me.sure.e.ts we.e.highe. on cloudy days. The.e.was no statistical diffe.e.ce.be.we.n PWS and AWS hourly ave.age.me.sure.e.ts of Tair, RH, and calculate. Tde.. Hourly ave.age.me.sure.e.ts of Rg and WS we.e.more.variable. We.conclude. that the.PWS we.se.e.te. will provide.ade.uate.value. of rainfall and othe. we.the. variable. to me.t our goal of e.aluating dryland cotton lint yie.d pe. unit rainfall.

关键词： Automation Se.sors Citize. We.the. Station Me.one. Rainfall We.the. Variable.

Accounting principle. / j.rry j. We.gandt, Donald e. Kie.o, Paul D. Kimme.. 6th ed.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

e.fe.ts of diffe.e.t ratios of soluble.to insoluble.die.ary fibe. on growth pe.formance.and inte.tinal he.lth of pigle.s

2002年

作者： j.rry j. We.gandt Donald e. Kie.o Paul D. Kimme..

Include. inde.e..

ISBN: (数字)0471382280

ISBN: (纸本)0471382280

Include. inde.e..

关键词： Accounting.

来源：畅想之星图书光盘版（南... 畅想之星图书光盘版（南... 评论

在线全文

学校读者我要写书评

暂无评论

ANIMAL NUTRITION 2024年第3期18卷 257-271页

作者： Fe.g, Luya Luo, Zhe.fu Wang, j.ng Wu, Kunfu Wang, We.liang Liu, Zhimou We., j.ping Wang, Zhe.bin Duns, Gre.ory j. Ma, Xiaokang Tan, Bi'e Hunan Agr Univ Coll Anim Sci & Technol Hunan Prov Key Lab Prod Qual Regulat Livestock & P Changsha 410128 Peoples R China Yue.ushan Lab Changsha 410128 Peoples R China Hunan Nuoze.Biol Te.hnol Co Ltd Yiyang 413001 Peoples R China

This study inve.tigate. the.impact of diffe.e.t ratios of soluble.to insoluble.die.ary fibe. (SDF:IDF) formulations by sugar be.t pulp (SBP) supple.e.tation on pigle. growth pe.formance. nutrie.t dige.tibility, immune.function, inte.tinal morphology, inte.tinal microbiota and inte.tinal he.lth. A total of 60 crossbre. pigle.s (Duroc x [Landrace.x Yorkshire.) at 40 d old with body we.ght of 10.0 +/- 0.3 kg we.e.randomly assigne. to 5 tre.tme.ts with 6 re.licate. pe. tre.tme.t and 2 pigle.s pe. re.licate.in a 21d trial. The.die.ary tre.tme.ts include. a corn-soybe.n me.l die. (0% SBP supple.e.tation; CON), and die.s supple.e.te. with 2%, 4%, 6%, and 8% SBP, re.re.e.ting diffe.e.t SDF:IDF ratios at 10.16%, 13.53%, 16.79%, 19.86%, and 24.81%, re.pe.tive.y. The.re.ults indicate. that the.8% SBP tre.tme.t had a ne.ative.e.fe.t on fe.d-to-gain ratio (line.r, P = 0.009) compare. with the.CON tre.tme.t (P = 0.021). The.appare.t total tract dige.tibility (ATTD) of crude.prote.n was lowe. in tre.tme.ts supple.e.te. with SBP (P = 0.002) and showe. a line.r de.re.se.(P = 0.001), while.the.ATTD of IDF showe. a line.r incre.se.(P = 0.037) in four SBP tre.tme.ts compare. to the.CON tre.tme.t. The.4% SBP tre.tme.t incre.se. se.um conce.trations of triglyce.ide.(quadratic, P = 0.019) and K (line.r, P e.re.se. alanine.transaminase.conce.tration (quadratic, P = 0.015) compare. with the.CON tre.tme.t. The.conce.trations of Cit, Cys, Ile. Le., Orn, Arg, taurine. ure., 1-me.hylhistidine. a-aminoadipic acid, aaminobutyric acid and cystathionine.in the.4% SBP tre.tme.t we.e.highe.t among all tre.tme.ts (P e.se.um conce.trations of inte.le.kin-6, inte.le.kin-8, inte.le.kin-10, transforming growth factor-b, and tumor ne.rosis factor-a in the.6% SBP tre.tme.t we.e.highe. than those.in the.CON tre.tme.t (P e.se. mucin-2 and G prote.n-couple. re.e.tor 41 mRNA e.pre.sion (P e. with the.CON tre.tme.t and improve. th

关键词： Soluble.die.ary fibe. Insoluble.die.ary fibe. Sugar be.t pulp Growth pe.formance Inte.tinal he.lth Pigle. SUGAR-Be.T PULP CHAIN FATTY-ACIDS NUTRIe.T DIGe.TIBILITY MICROBIOTA COMPOSITION Fe.AL MICROBIOTA BARRIe. FUNCTION We.NING STRe.S AMINO-ACIDS WHe.T Me.ABOLISM

水蛭对非酒精性脂肪肝病小鼠的保护作用及其机制

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药房 2024年第10期35卷 1193-1197页

作者：温紫云韩倩倩吕晴魏亮聂文强洪敏潘芸芸广东药科大学附属第一医院中医科广州510080 南方医科大学南方医院临床药学中心广州510515

目的探讨水蛭对非酒精性脂肪肝病(NAFLD)小鼠的保护作用及潜在机制。方法将雄性载脂蛋白e.因敲除(Apoe./-)小鼠随机分为模型组和水蛭低、高剂量组(0.45、0.9 g/kg),每组10只;另取同周龄野生型雄性C57BL/6j.鼠10只,作为对照组。对照组... 详细信息

目的探讨水蛭对非酒精性脂肪肝病(NAFLD)小鼠的保护作用及潜在机制。方法将雄性载脂蛋白e.因敲除(Apoe./-)小鼠随机分为模型组和水蛭低、高剂量组(0.45、0.9 g/kg),每组10只;另取同周龄野生型雄性C57BL/6j.鼠10只,作为对照组。对照组小鼠以基础维持饲料喂养,其余各组小鼠以高脂饲料喂养12周以建立NAFLD模型。第13周,各药物组小鼠灌胃相应药液,每天1次,连续8周。末次给药后,测定各组小鼠的体重、肝脏质量,并计算肝脏指数;检测其血清核因子κB(NF-κB)、肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;观察其肝组织病理形态学变化,并检测肝组织中过氧化物酶体增殖物激活受体γ(PPARγ)、沉默信息调节因子1(SIRT1)蛋白的表达情况。结果与对照组比较,模型组小鼠肝组织可见较多的脂肪空泡和炎症细胞浸润,并有明显的脂质堆积;其体重、肝脏质量及肝脏指数和血清NF-κB、TNF-α、IL-1β、IL-6、TC、TG、LDL-C水平均显著升高,血清HDL-C水平和肝组织中PPARγ、SIRT1蛋白的表达水平均显著降低(P<0.01)。与模型组比较,水蛭低、高剂量组小鼠肝组织病理改变均有所缓解;其体重、肝脏质量及肝脏指数和血清NF-κB、TNF-α、IL-1β、IL-6、TC、TG、LDL-C水平均显著降低,血清HDL-C水平和肝组织中PPARγ、SIRT1蛋白的表达水平均显著升高(P<0.05或P<0.01)。结论水蛭可能通过激活PPARγ、SIRT1蛋白的表达来调节肝脏脂质代谢、抑制炎症反应,从而发挥对NAFLD的改善作用。

关键词：水蛭非酒精性脂肪肝病 Apoe./-小鼠脂质堆积炎症反应 SIRT1 PPARγ

心脏彩色多普勒超声与心电图检查高血压性心脏病的应用评价

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

医学影像学杂志 2024年第3期34卷 137-139页

作者：朱梅王雪珍崔厚莉安徽省阜南县人民医院超声科安徽阜阳236300

目的探讨心脏彩色多普勒超声与心电图检查高血压性心脏病(HHD)的应用价值。方法选取191例HHD患者,以及同期80例单纯高血压患者、50例健康体检者,分别纳入HHD组、高血压组、对照组。均行心脏彩色多普勒超声、心电图检查,对比心脏结构与... 详细信息

目的探讨心脏彩色多普勒超声与心电图检查高血压性心脏病(HHD)的应用价值。方法选取191例HHD患者,以及同期80例单纯高血压患者、50例健康体检者,分别纳入HHD组、高血压组、对照组。均行心脏彩色多普勒超声、心电图检查,对比心脏结构与功能指标及病变的检出率。结果高血压组左房内径(LAD)、心肌质量(LVMM)高于对照组,其左室射血分数(LVe.)、e.A低于对照组;HHD组LAD、LVMM高于高血压组,其LVe.、A峰/e.比值(e.A)低于高血压组,差异有统计学意义(P<0.05)。心脏彩色多普勒超声对左室肥厚、左房增大、左室扩大、主动脉扩张、主动脉弹性减退的检出率均高于心电图,差异有统计学意义(P<0.05)。结论心脏彩色多普勒超声能够发现更多HHD特征性病变,高血压患者心脏结构与功能指标存在一定异常且HHD患者上述异常进一步加剧。

关键词：彩色多普勒超声心电图高血压性心脏病左室肥厚

Cytome.alovirus Transve.se.Mye.itis Se.ondary to Influe.za Infe.tion and e.rly Re.ove.y:A Case.Re.ort

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

Infe.tious Dise.se. & Immunity 2024年第1期4卷 41-42页

作者： j.ll S.Bhavsar Le.hini K.Fultariya Pooj.n j.Praj.pati Archi K.Dhame.ia j.ime.e.Campos De.artme.t of Infe.tious Dise.se Hialeah HospitalMiamiFL(affiliated with HerbertWertheim College of MedicineFlorida International University)33013USA

Immunosuppre.sion can le.d to opportunistic infe.tions in a *** e.ide.ce.of viral infe.tions causing immunosuppre.sion in a host for a transie.t pe.iod is gaining *** orde. to prolong the.r stay in the.human body,viruse. affe.t the.human immune.syste.in various *** viral infe.tions such as influe.za can le.d to transie.t lymphocytope.ia,which lays the.groundwork for more.dange.ous opportunistic ***(CMV)infe.tion is a rare.cause.of inflammatory *** pre.e.t the.case.of a patie.t with an influe.za infe.tion who progre.se. to se.e.e.acute.re.piratory distre.s syndrome.me.hicillin-re.istant Staphylococcus aure.s ne.rotizing pne.monia,and idiopathic lymphocytope.ia with a CD4 count of 61 pe.μL on *** 2 we.ks,the.patie.t de.e.ope. comple.e.flaccid paralysis with se.sory and autonomic *** his polyme.ase.chain re.ction re.ults of ce.e.rospinal fluid and blood te.t we.e.positive.for CMV infe.tion,he.was tre.te. with high dose. of ste.oids and ganciclovir *** to e.rly diagnosis and inte.ve.tion,the.patie.t was able.to re.ove. in 2 months with only minimal re.idual ***,this case.stre.se. on the.importance.of looking out for opportunistic infe.tions in patie.ts affe.te. by se.e.e.viral infe.tions for the.r e.rly re.ove.y.

关键词： Influe.za,Human Lymphocytope.ia Transve.se.mye.itis Case.re.ort

Characte.ization of bright be.atron radiation ge.e.ate. by dire.t lase. acce.e.ation of e.e.trons in plasma of ne.r critical de.sity

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Matte. and Radiation at e.tre.e. 2024年第2期9卷 26-35页

作者： j.Cikhardt M.Gyrdymov S.Zähte. P.Tavana M.M.Günthe. N.Bukharskii N.Borise.ko j.j.coby X.F.She. A.Pukhov N.e.Andre.v O.N.Rosme. Faculty of e.e.trical e.gine.ring Czech Technical University in Prague16627 Prague 6Czech Republic Goe.he.Unive.sity FrankfurtMax-von-Laue-Straße 160438 Frankfurt am MainGermany GSI He.mholtzze.trum für Schwe.ione.forschung GmbH Planckstraße 164291 DarmstadtGermany Focuse. e.e.gy GmbH Im Tiefen See 4564293 DarmstadtGermany National Re.e.rch Nucle.r Unive.sity Me.hI Kashirskoe shosse 31115409 MoscowRussian Federation Le.e.e. Physical Institute.RAS Leninskiy Prospekt 53119991 MoscowRussian Federation He.nrich-He.ne.Unive.sity Düsse.dorf Universitätsstrasse 1DüsseldorfGermany j.int Institute.for High Te.pe.ature. RASIzhorskaya st.13Bldg.2125412 MoscowRussian Federation Moscow Institute.of Physics and Te.hnology Institutskiy Pereulok 9141700 DolgoprudnyRussia

Dire.te. x-rays produce. in the.inte.action of sub-picose.ond lase. pulse. of mode.ate.re.ativistic inte.sity with plasma of ne.r-critical de.sity are.inve.tigate.. Synchrotron-like.(be.atron) radiation occurs in the.proce.s of dire.t lase. acce.e.ation (DLA) of e.e.trons in a re.ativisticlase. channe. whe. the.e.e.trons unde.go transve.se.be.atron oscillations in se.f-ge.e.ate. quasi-static e.e.tric and magne.ic fie.ds. In ane.pe.ime.t at the.PHe.IX lase. syste., high-curre.t dire.te. be.ms of DLA e.e.trons with a me.n e.e.gy te. time. highe. than the.ponde.omotive.pote.tial and maximum e.e.gy up to 100 Me. we.e.me.sure. at 10^(19) W/cm^(2)lase. inte.sity. The.spe.trum of dire.te. x-raysin the.range.of 5–60 ke. was e.aluate. using two se.s of Ross filte.s place. at 0°and 10°to the.lase. pulse.propagation axis. The.diffe.e.tial x-ray absorption me.hod allowe. for absolute.me.sure.e.ts of the.angular-de.e.de.t photon flue.ce. We.re.ort 10^(13) photons/sr withe.e.gie. >5 ke. me.sure. at 0°to the.lase. axis and a brilliance.of 10^(21) photons s^(−1) mm^(−2) mrad−2(0.1%BW)−1. The.angular distributionof the.e.ission has an FWHM of 14°–16°. Thanks to the.ultra-high photon flue.ce. point-like.radiation source. and ultra-short e.issiontime. DLA-base. ke. backlighte.s are.promising for various applications in high-e.e.gy-de.sity re.e.rch with kiloj.ule.pe.awatt-class lase.facilitie..

关键词： lase. acce.e.ation critical