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Analysis of metabolic alterations in Arabidopsis following changes in the carbon dioxide and oxygen partial pressures

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journal of Integrative Plant Biology 2014年第9期56卷 941-959页

作者： Alexandra Florian Stefan timm Zoran Nikoloski Takayuki Tohge Hermann Bauwe Wagner L.Araujo Alisdair R.Fernie Max Planck Institute for Molecular Plant Physiology Department of Plant Physiology University of Rostock Max-Planck Partner group at the Departament of Plant Biology Federal University of Vicosa

As sessile organisms,plants are subject to a multitude of environmental variations including several which directly affect their interaction with the *** the indiscriminant nature of Rubisco,the relative rates of photosynthesis and photorespiration are known to be responsive to changes in gas ***,comprehensive profiling methods have not yet been applied in order to characterize the wider consequences of these changes on primary metabolism in ***,although transcriptional profiling has revealed that a subset of photorespiratory enzymes are co-expressed,whether transcriptional responses play a role in short-term responses to atmospheric compositional changes remains *** address these questions,plants Arabidopsis thaliana（Arabidopsis） ecotype Columbia（Col-O） grown under normal air conditions were transferred to different CO_2 and O_2 concentrations and characterized at the physiological,molecular,and metabolic levels following this *** results reveal alterations in the components,which are directly involved in,or supporting,photorespiration,including transcripts and metabolite *** results further highlight that the majority of the regulation of these pathways is not mediated at the level of transcription and that the photorespiratory pathway is essential also in conditions in which flux through the pathway is minimized,yet suggest that flux through this pathway is not mediated at the level of transcription.

关键词： Carbon dioxide partial pressures gene expression metabolic profiling oxygen partial pressures

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ger

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2012年

作者： (德) Angelika Becker-Kavan jeffrey Lloyd jochen timm

本书以四位职员的有趣故事为线索贯穿于10个单元。第一单元是启蒙动力期, 帮助学生迈入英语学习的第一步。在其他单元中精选案例教学, 寓教于乐, 将工作和社会交际轻松相结合, 教会学生使用恰当的专业技术语言。每个单元包含语言部分、... 详细信息

ISBN: (纸本)9787894335951;9787111391616

本书以四位职员的有趣故事为线索贯穿于10个单元。第一单元是启蒙动力期, 帮助学生迈入英语学习的第一步。在其他单元中精选案例教学, 寓教于乐, 将工作和社会交际轻松相结合, 教会学生使用恰当的专业技术语言。每个单元包含语言部分、网络资源、词汇部分、语法部分和学习建议五个部分。

关键词：机械工程

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Wheelchair Seating: Peak Pressure Distribution in Young versus Elderly Healthy Controls

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Open journal of Therapy and Rehabilitation 2019年第2期7卷 25-34页

作者： Marina timm Kersti Samuelsson Center of Assistive Devices County Council Sk&#246 vde Sweden Department of Rehabilitation Medicine and Department of Medical and Health Sciences Link&#246 ping University Link&#246 ping Sweden

Background: People may use a wheelchair when they need assistance with mobility. Some users have to remain seated in their wheelchair for most of their awake time and for a lot of activities. The need for a well fitted and supportive wheelchair is important and, for those who use the wheelchair for many hours, peak pressure distribution is of interest to prevent pressure ulcers and discomfort. Aim: To describe how high local peak pressures may be distributed over risk areas for pressure ulcers in different wheelchair settings. Methods: Two groups of healthy adults were recruited (40 elderly and 30 younger individuals). Two standard models of manual wheelchairs were used together with two types of wheelchair cushions. Results: The elderly group had a higher peak pressure over the ischial tuberosity and increased posterior pelvic rotation in the shaped seat cushion. Both groups had higher peak pressure over the trochanter in the shaped seat cushion. Peak pressure was also higher over the coccyx for the elderly in the shaped seat cushion compared with the plain cushion;this was lower for the younger group. Discussion: The results indicate that age might influence the important physical prerequisites when fitting a wheelchair to an individual.

关键词： Age Interface Pressure Pressure Ulcers Prevention

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Photorespiration Is Crucial for Dynamic Response of Photosynthetic Metabolism and Stomatal Movement to Altered C02 Availability

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Molecular Plant 2017年第1期10卷 47-61页

作者： Marion Eisenhut Andrea Brautigam Stefan timm Alexandra Florian Takayuki Tohge Alisdair R. Fernie Hermann Bauwe Andreas P.M. Weber Institute of Plant Biochemistry Cluster of Excellence on Plant Sciences Heinrich Heine University 40225 Dusseldorf Germany Department of Plant Physiology University of Rostock Albert-Einstein-StraBe 3 18051 Rostock Germany Department of Molecular Physiology Max-Planck Institute for Molecular Plant Physiology 14476 Potsdam-Golm Germany Present address: IPK Gatersleben Network Analysis and Modeling Corrensstrasse 3 06466 Seeland Germany

The photorespiratory pathway or photorespiration is an essential process in oxygenic photosynthetic or- ganisms, which can reduce the efficiency of photosynthetic carbon assimilation and is hence frequently considered as a wasteful process. By comparing the response of the wild-type plants and mutants impaired in photorespiration to a shift in ambient C02 concentrations, we demonstrate that photorespiration also plays a beneficial role during short-term acclimation to reduced C02 availability. The wild-type plants re- sponded with few differentially expressed genes, mostly involved in drought stress, which is likely a conse- quence of enhanced opening of stomata and concomitant water loss upon a shift toward low C02. In contrast, mutants with impaired activity of photorespiratory enzymes were highly stressed and not able to adjust stomatal conductance to reduced external C02 availability. The transcriptional response of mutant plants was congruent, indicating a general reprogramming to deal with the consequences of reduced C02 availability, signaled by enhanced oxygenation of ribulose-l,5-bisphosphate and amplified by the artificially impaired photorespiratory metabolism. Central in this reprogramming was the pro- nounced reallocation of resources from growth processes to stress responses. Taken together, our results indicate that unrestricted photorespiratory metabolism is a prerequisite for rapid physiological acclimation to a reduction in C02 availability.

关键词： photorespiration starvation stress stomata transcription Arabidopsis thaliana

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E-selectin ligand complexes adopt an extended high-affinity conformation

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journal of Molecular Cell Biology 2016年第1期8卷 62-72页

作者： Roland C.Preston Roman P.jakob Florian P.C.Binder Christoph P.Sager Beat Ernst timm Maier Institute of Molecular Pharmacy Universitat Basel4056 BaselSwitzerland Biozentrum Universitat Basel4056 BaselSwitzerland

E-selectin is a cell-adhesion molecule of the vascular endothelium that promotes essential leukocyte rolling in the early inflammatory response by binding to glycoproteins containing the tetrasaccharide sialyl Lewisx(sLex).Efficient leukocyte recruitment under vascular flow conditions depends on an increased lifetime of E-selectin/ligand complexes under tensile force in a so-called catch-bond binding ***-crystal structures of a representative fragment of the extracellular E-selectin region with sLex and a glycomimetic antagonist thereof reveal an extended E-selectin conformation,which is identified as a high-affinity binding state of E-selectin by molecular dynamics ***-angle X-ray scattering experiments demonstrate a direct link between ligand binding and E-selectin conformational transition under static conditions in *** permits tracing a series of concerted structural changes connecting ligand binding to conformational stretching as the structural basis of E-selectin catch-bond-mediated leukocyte *** detailed molecular view of the binding site paves the way for the design of a new generation of selectin *** is of special interest,since their therapeutic potentialwas recently demonstrated with the pan-selectin antagonists GMI-1070(Rivipansel).

关键词： inﬂammation leukocyte adhesion E-selectin glycomimetic antagonist sialyl Lewisx conformational change

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Mobilization of Ti-Nb-Ta during subduction:Evidence from rutile-bearing dehydration segregations and veins hosted in eclogite,Tianshan,NW China

Mobilization of Ti-Nb-Ta during subduction:Evidence from rut...

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中国科学院地质与地球物理研究所2007学术年会

作者： timm john Reiner Klemd Physics of Geological Processes PGP University of Oslo Institut für Geowissenschaften and SFB 574 Universitt KielOlshausenstr.4024098 KielGermany Mineralogisches Institut Universitt W(u|¨)rzburgAm Hubland 97074WürzburgGermany

Field evidence from the western Tianshan subduction complex in northwestern China indicates that the high field strength elements Ti,Nb,and Ta were mobilized and thereby fractionated from Zr and Hf during the dehydration process that transformed blueschist into *** a segregation with a depletion halo,thought to represent initial mobilization during dehydration,and a transport vein,indicative of the long distance transport were *** each case,centimeter-sized rutile grains grew as needle-like crystals in the segregation and as prismatic crystals in the *** the host rock of the segregation,the Ti contents of garnet and omphacite,the modal abundances of rutile and titanite and the bulk rock Ti,Nb, and Ta contents decrease towards the *** observations are consistent with transport of Ti,Nb,and Ta from the host rock into the *** and geochemical data for the eclogite-facies vein minerals indicate that Ti-Nb-Ta -rich fluids were transported over long-distances(at minimum meter-scale) during fracture-controlled fluid *** forming ligands(e.g.,Na-Si-Al polymers and F) may have enhanced the solubility of Ti,Nb,and Ta in the *** in fluid composition(e.g.,X) may both precipitate rutile and fractionate Ti,Nb,and Ta from LILE and REE.

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Is There a Metabolic Requirement for Photorespiratory Enzyme Activities in Heterotrophic Tissues？

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Molecular Plant 2014年第1期7卷 248-251页

作者： Adriano Nunes-Nesi Alexandra Florian Andrew Howden Kathrin jahnke Stefan timm Hermann Bauwe Lee Sweetlove Alisdair R. Fernie Max Planck Partner Group Departamento de Biologia Vegetal Universidade Federal de Vicosa 36570-000 Vicosa Minas Gerais Brazil Central Metabolism Group Max Planck Institute of Molecular Plant Physiology Am Muehlenberg 1 D-14476 Potsdam-Golm Germany Department of Plant Sciences University of Oxford South Parks Road Oxford OX2 3RB UK Department of Plant Physiology University of Rostock D-18051 Rostock Germany

Photorespiration is an essential metabolic process in leaves that facilitates recovery of carbon lost by the oxygenase reaction of Rubisco and avoids the accumulation of the toxic product, 2-phosphoglycolate （2PG） of this reaction （Bauwe et al., 2012）. However, there is also evidence to suggest that photorespiration has a more complex role during normal growth than the mere detoxification of 2PG and the recovery of 3-phosphoglycerate （3PGA）（Bauwe et al., 2012）.

关键词： HETEROTROPHIC bacteria RESEARCH TISSUES PLANT photorespiration GLYCOLATES PLANT enzymes PLANT enzymes -- Regulation

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Observing the jumping Laser Dogs

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journal of Applied Mathematics and Physics 2016年第11期4卷 1977-1988页

作者： Alberto Tufaile timm A. Vanderelli Adriana Pedrosa Biscaia Tufaile Soft Matter Laboratory Escola de Artes Ciências e Humanidades Universidade de S&atildeo Paulo S&atildeo Paulo Brazil Ferrocell USA 739 Route 259 Ligonier PA USA

jumping sun dogs are rapid light flashes changing over clouds, with some of them located close to the places of halo formation in thunder storms clouds. This paper presents an outline of some aspects that are required for understanding the jumping sun dogs, using some experiments with light scattering in complex fluids. In our analogy, we have observed the jumping laser dogs, in which the ice crystals are replaced by needlelike structures of ferrofluid, the electric field in the atmosphere is represented by an external magnetic field, and the laser beam scattered by the ferrofluid structure has the same role of the sun as the source of light scattered by the ice crystals subjected to changing electric fields in thunderstorm clouds.

关键词： Diffracted Rays Sun Dogs Laser Dogs Geometrical Theory of Diffraction

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弹性地质温压计的合理应用:以石榴子石中石英包裹体压力计为例

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岩石学报 2022年第10期38卷 2933-2948,I0001-I0012页

作者：仲歆王浩铮冯林峰张华锋陈小宇 jOHN timm 张丁丁翟明国柏林自由大学地球科学学院柏林122492 西南石油大学地球科学与技术学院成都610500 中国地质大学(北京)地球科学与资源学院北京100083 中国地震局地质研究所北京100029 中国科学院地质与地球物理研究所北京100029

压力和温度是利用岩石学研究地质过程的核心参数。传统的计算岩石温压条件的方法常要求矿物间达到化学平衡,如相平衡和矿物化学温压计。但是,自然界一些岩石中的矿物组合不一定能达到平衡。基于矿物弹性开发的地质压力计则不受这一条件... 详细信息

压力和温度是利用岩石学研究地质过程的核心参数。传统的计算岩石温压条件的方法常要求矿物间达到化学平衡,如相平衡和矿物化学温压计。但是,自然界一些岩石中的矿物组合不一定能达到平衡。基于矿物弹性开发的地质压力计则不受这一条件的影响,它的原理是利用矿物包裹体中保存的残余应力来恢复其被捕获时的压力条件。这种方法能简单快速地获取数据,以实现对温度压力的限定。目前,最常使用石榴子石中的石英包裹体及其弹性地质压力计来确定包裹体的压力。弹性地质压力计的应用与其它常规方法不同之处,在于如何选择包裹体和弹性模型用以计算捕获时的压力条件。准确应用弹性地质压力计的重点在于样品制备、拉曼光谱测量、拉曼光谱处理和弹性模型的建立与选择。同时,本文编制了计算与应用更为便捷的Excel计算表格,将测量石英包裹体的特征拉曼峰峰位直接输入Excel计算表格中即可计算出被捕获时的压力条件。以大别-苏鲁造山带中烟台郭城地区榴辉岩为例,使用弹性地质压力计获得了石榴子石生长的压力史,且与传统地质温压计的结果相对一致。本文提供的方法将为进一步限定相关地质过程中岩石压力记录和变化过程提供新的路径。

关键词：地质温压计拉曼光谱弹性力学矿物包裹体石英

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Flaw Detection Capabilities in Aerospace with Eddy Current Array Technology

Flaw Detection Capabilities in Aerospace with Eddy Current A...

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2018远东无损检测新技术论坛

作者： jesse HERRIN Nicholas CARDILLO Steve timm Tom ROHLFING Zetec

Eddy current array(ECA) has enhanced the practice of flaw detection in the aerospace industry through expanded coverage areas, reduced inspection time, improved detectability, repeatability, and data recording. However, because ECA detectability is inversely proportional to scan speed, the signal-to-noise ratio(SNR) of today’s ECA techniques limits their ability to detect the very small flaws and loss of material that can occur in many aerospace inspections such as friction stir welds and multi-layer structures susceptible to corrosion and fatigue cracking. Minimum flaw size and required data density are the main factors affecting scan speed when using eddy current array. As speed increases, the SNR decreases, therefore the minimum flaw size becomes larger. Zetec introduces exceptional SNR in an eddy current array instrument, eliminating the need to sacrifice scan speed for superior flaw detectability.

关键词： eddy current array EC ECA SNR POD

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