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A reversed-phase HPlC-UV assay for simultaneous analysis of EgCg and ECg of tea polyphenols in rat plasma

沈阳药科大学学报 2008年第S1期25卷 99-99页

作者： FU Ting1,lIAng jun1,HAn guo-zhu1,l li1,lI nan2(1.Department of Clinical Pharmacology,Dalian Medical University,Dalian 116044,China 2.Department of Analytical Chemistry,Dalian University of Technology,Dalian 116023,China)

Objective To develop a simple and specific reversed-phase HPlC-UV method for simultaneous determination of(-)Epig.llocatechin-3-g.llate(EgCg)and(-)Epicatechin-3-g.llate(ECg),the main active ingredients of tea polyphenols(TP),in rat *** EgCg and ECg were eluted on a Kromasil C18 analytical column(150 mm×4.6 mm,5 μm)protected by a C18 pre-column(4.6 mm×20 mm,10 μm)with a linear gradient mobile phase composed of CH3Cn(A)-0.1% citric acid(B),which was run from initial 14% A and 86% B to 20% A and 80% B at a flow rate of 1.0 ml·min-1 in 14 min,then changed to 14% A and 86% B at a gradient flow rate of 1.0-1.5 ml·min-1 during 14-18 min,and then maintained until 22 min at a gradient flow rate of 1.5-1.0 ml·*** UV detector was set at 280 *** samples(200 μl each)were prepared by liquid-liquid extraction procedure with double volumes of EtoAc and then evaporation of organic phase under n2 stream to dry,followed by reconstitution with 100 μl of 20% CH3Cn aqueous *** peak area ratios of analytes to vanillin as internal standard vs concentration of analytes to construct calibration *** The HPlC resulted in base-line separation of vanillin,EgCg,ECg and other components;there was no interference from blank *** linear range was 0.5-300 μg·ml-1 for EgCg(r=0.9999)and 0.1-60 μg·ml-1 for ECg(r=0.9999).The intra-and inter-day precision(RSD)was better than 6.1% and 12.6%,respectively,and the average accuracy was between 86.25%-103.14%.The extraction recovery of EgCg and ECg was 79.80%-84.64% and 75.22%-91.39%,*** plasma samples were stable for at least 30 days at-20 ℃ and 8 h at room temperature;EgCg,ECg and IS stock solutions 2 months at-20 ℃,and the EtoAc-extracted plasma samples 24 h at 4 ℃.Application of the method to the determination of EgCg and ECg in plasma of rats receiv.ng iv 100 mg·kg-1 of TP showed that these 2 compounds pharmacokinetically behaved as the two-compartment model and first-order kinetics,with t1/2β 1

关键词： HPlC tea polyphenols EgCg ECg

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单剂量丙泊酚注射对慢性每日头痛患者疼痛和生活质量的影响：一项随机双盲对照试验

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麻醉与镇痛 2011年第5期7卷 34-43页

作者： Mark K. Simmonds, MB ChB Saifudin Rashiq, BM BS Igor A. Sobolev, MD Bruce D. Dick, PhD Daniel P. gray, MD Bradley j. Stewart, MD Kathryn I. jamieson-lega, BSc 苏海华（译） Department of Anesthesiology and Pain Medicine University of Alberta 600 Hys Centre Edmonton Alberta Canada 不详

背景有少量的病例研究提倡静脉输注丙泊酚来治疗慢性每日头痛（chronicdailyheadache；CDH），然而，尚无该疗法的随机对照试验。在本随机双盲对照试验中，我们的目的是观察单剂量的静脉丙泊酚输注（2．4mg＆g）能否显著降低CDH患者临... 详细信息

背景有少量的病例研究提倡静脉输注丙泊酚来治疗慢性每日头痛（chronicdailyheadache；CDH），然而，尚无该疗法的随机对照试验。在本随机双盲对照试验中，我们的目的是观察单剂量的静脉丙泊酚输注（2．4mg＆g）能否显著降低CDH患者临床不适及接下来30天的疼痛。方法符合标准的CDH成人患者，分别静脉输注丙泊酚（n=20）或安慰剂咪达唑仑（n=20）对照组。主要的结果评估包括：①治疗30天后头痛失劳动力调查评分（HeadacheDisabilityInventory；HDI）；②头痛指数（HeadacheIndex，HI），治疗期间30天的头痛强度总评估；③采用用药量化评分表（MedicationQuantificationScale，第1II版）评估镇痛药消耗量。结果输注丙泊酚30天后，HDI降低了9．47点（标准差14．1）（P=0．009），但是其降低程度比认为头痛相关失劳动力调查指数（HDI）有临床意义的降低程度更小。与对照组相比，HDI无显著统计差异。在降低平均头痛强度（用头痛指数评估）或用药量化表（MedicationQuantification Scale）（第111版）方便，组内及两组间无统计学差异。结论单剂量的丙泊酚静脉输注（2．4mg/kg）产生了有统计学意义的变化，但是在输注药物后30天CDH失劳动力方面的改变无临床意义。丙泊酚也不能减轻头痛强度和减少镇痛药的应用。本研究不支持静脉输注丙泊酚临床治疗CDH。

关键词：随机双盲对照试验慢性每日头痛成人患者单剂量生活质量疼痛酚注射静脉输注

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伴脑神经受累的慢性脱髓鞘性多神经病变与抗MAg/SgPg/Sg.lg抗体相关(法)

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世界核心医学期刊文摘(神经病学分册) 2006年第11期 41-41页

作者： Drouet A Caudie C Vallat j.M 赵天智 Service de neurologie HIA Desgenettes 108 boulevard Pinel 69275 lyon Cedex 03 France

Introduction. A typically distal and symmetrical, slowly progressive sensorimotor demyelinating neuropathy is caused by monoclonal IgM against myelin-associated glycoprotein (MAg) and SgPg, SglPg glycolipids in the context of a benign IgM paraproteinemia. We studied a patient with a neuropathy that fulfilled the diagnostic criteria for CIDP in whom IgM kappa anti-MAg/SgPg/SglPg were detected. Observation. The patient was a 57-year-old man who had developed a slowly progressive distal sensorimotor neuropathy, involving the lower then upper limbs, with cranial nerves palsies (oro-pharyngo-laryngo territory). EnMg showed a demyelinating neuropathy with a disproportionate slowing of conduction in distal segments of motor and axonal features in the lower limbs. The first routine laboratory analysis revealed negative or normal findings. Several serum protein electrophoreses were normal. The third cerebrospinal fluid examination demonstrated a moderate and late rise in CSF protein level with no cells. Monoclonal IgM-kappa against MAg/SgPg/SglPg, was detected; anti-MAg antibody titre in the serum was 20 059 BTU (n ll IgM-kappa paraprotein was identified by immunofixation. Electron microscopy failed to show nerve fibers with widening of outer lamellae of the myelin. There is no clinical improvement after different treatments, immunoglobulins iv, cortisteroids, plasma exchange, rituximab. Conclusion. It is not known whether this neuropathy is an atypical form of PnMAg or an CIDP associated with anti-MAg. When EnMg show a disproportionate slowing of conduction in distal segments of motor nerves, one should screen the serum with immunofixation to identify small monoclonal components. If IgM-MgUS is present, search should be undertaken for anti-MAg/SgPg/SglPg antibodies. Diagnosis enables optimal treatment using, in severe cases, expensive current strategies with immunoglobulins iv, plasma exchange, and corticosteroids, or, in the event of no response, rituximab bef

关键词：脱髓鞘性神经病变 MAg/SgPg/Sg.lg 抗体

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急性卒中护理质量的改善:纽约州指定卒中中心计划

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世界核心医学期刊文摘(神经病学分册) 2006年第12期 15-15页

作者： gropen T.I. gagliano P.j. Blake C.A. 郭俊 Dr.neurology Department Long Island College Hospital339 Hicks St.BrooklynNY 11201United States

BACKgROUnD:Many hospitals lack the infrastructure required to treat patients with acute *** Brain Attack Coalition(BAC)published guidelines for the establishment of primary stroke ***:To determine if stroke center designation and selective triage of acute stroke patients improve quality of ***:Baseline chart abstraction was performed on all stroke patients admitted to 32 hospitals serving Brooklyn and Queens,nY,from March to May *** were invited to meet BAC guideline-based *** was verified by on-site *** designation,acute stroke patients were selectively *** data were collected from August to October ***:The authors abstracted 1,598 charts at baseline and 1,442 charts at *** baseline to remeasurement,median times decreased for door to physician contact(25 vs 15 minutes,p = 0.001),CT performance for potential tissue plasminogen activator(t-PA)candidates(68 vs 32 minutes,p nd t-PA administration(109 vs 98 minutes,p = nS).iv t-PA utilization increased from 2.4 to 5.2%(p lect t-PA protocol violations decreased from 11.1 to 7.9%(p = nS),and the stroke unit admission rate increased from 16 to 39%(p n stroke centers(n = 14)vs nondesignated hospitals(n = 18),there were shorter median times from door to physician contact(10 vs 25 minutes,p nce for potential t-PA candidates(31 vs 40 minutes,p = nS),and t-PA administration(95 vs 115 minutes,p nters,compared with nondesignated centers,admitted acute stroke patients to stroke units more often(55.9 vs 10.9%,p nClUSIOnS:Stroke center designation and selective triage of acute stroke patients improved the quality of care,including access to timely thrombolytic therapy and stroke units.

关键词：卒中中心患者急性卒中护理质量纽约州

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使用紫杉醇-铂类预处理的卵巢癌患者二线治疗中疾病稳定状况是否为存活的有效指标

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世界核心医学期刊文摘（妇产科学分册） 2005年第1期1卷 50-50页

作者： gronlund B. Hdall C. Christensen I.j. 王刚 Department of Onco logy The Finsen CenterRigshospitalet Copenhagen Univ. H. Copenhagen Denmark

Objective. Recurrent ovarian carcinoma is considered an incurable dis ease and second-line chemotherapy is administered for extension of survival and palliati on. The impact of continued antineoplastic treatment in patients with stable dis ease without a demonstrable response is uncertain. The aim of this analysis was to assess the value of a stabilization of the tumor size in second-line chemoth erapy as an indicator of survival. Methods. Retrospective, single-institution s tudy of 487 consecutive patients with primary epithelial ovarian carcinoma. Incl usion criteria: (1) FIgO stage IC-iv epithelial ovarian carcinoma; (2) first-l ine chemotherapy with Paclitaxel and a Platinum-compound; (3) refractory, persi stent, or recurrent disease diagnosed by imaging methods; and (4) intravenous se cond-line chemotherapy with single Topotecan or Paclitaxel-Carboplatin. Univar iate and multivariate analyses of survival with theWorld Health Organization (WH O) tumor response parameter included as a time-dependent variable were performe d. Results. The response rates were (n = 100): complete response (CR) 27%, part ial response (PR) 14%, stable disease (SD) 41%and progressive disease (PD) 18 %. In a multivariate Cox regression analysis of survival, SD was found to be an independent prognostic factor for survival and the death hazard ratio was 0.37 (SD versus PD; 95%CI: 0.16-0.86; P = 0.02). There was no statistically signifi cant difference in survival between patients with PR and SD (P = 0.09). Conclusi on. In secondline chemotherapy of ovarian cancer,patients demonstrating SD have a survival benefit compared to patients with PD measured by theWHO tumor respons e criteria.

关键词：二线治疗复发性卵巢癌肿瘤预后生存分析生存优势一线化疗独立预后因素影像学诊断多变量回归持续性

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结肠直肠黏液癌与印戒细胞癌的10年效果评价

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世界核心医学期刊文摘（胃肠病学分册） 2005年第11期1卷 11-11页

作者： O'Connell j.B. Maggard M.A. C.Y. Ko 成虹 Department of Surgery David Geffen School of Medicine at UCLA 72- 215 CHS 10833 Le Conte Avenue Los Angeles CA 90095 United StatesDr.

PURPOSE: Most studies examining mucinous or signet-ring cell colorectal cancers are single institution reports. This study used a national cancer registry to analyze the epidemiology and survival outcomes of these two subtypes of colorectal cancer compared with adenocarcinoma tumors. METHODS: All patients diagnosed with mucinous (n = 16,991), signet-ring cell (n = 1,522), or adenocarcinoma (n = 146,115) colorectal cancer in the Surveillance, Epidemiology, and End Results database (1991- 2000) were evaluated. Analyses were performed to obtain age-adjusted incidence rates, stage at presentation, tumor grade, and five-year relative survival for each subtype. RESUlTS: Mucinous were slightly morin females (53.4 percent). Incidence rates per 100,000 persons were: mu-cinous, 5.5; signet-ring cell, 0.6; and adenocarcinoma 46.6. The annual percent change during ten years was stable for mucinous, increased for signet-ring cell (4.8 percent; P nd decreased for adenocarcinoma (- 1.1 percent; P nous (18 percent) and signet-ring cell (21 percent) tumors were located in the rectum compared with adenocarcinoma (29 percent). Signet-ring cell presented at later stage (III/iv, 80.9 percent) more often than mucinous (52.8 percent) and adenocarcinoma (49.5 percent), and also had worse tumor grade (high grade: signet-ring cell, 73.5 percent; mucinous, 20.9 percent; adenocarcinoma, 17.5 percent). Rela-tive five-year survival was worse for signet-ring cell than mucinous or adenocarcinoma. COnClUSIOnS: We present a large population-based review of colorectal cancer subtypes by analyzing national data from the past decade. Although the incidence of colorectal adenocarcinoma is decreasing in the United States, mucinous and signet-ring cell subtypes are stable and increasing, respectively. Importantly, it seems that the signet-ring cell subtype has worse outcomes, whereas survival rates for mucinous tumors are similar to adenocarcinomas.

关键词：印戒细胞癌结肠直肠效果评价黏液癌大肠腺癌肿瘤分级肿瘤分期晚期肿瘤数据分析百分比变化

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Hiv-HCV共同感染患者肝移植术后HCV复发和肝脏线粒体损害

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世界核心医学期刊文摘（胃肠病学分册） 2005年第7期1卷 52-52页

作者： Duclos-Vallée j.- C. Vittecoq D. Teicher E. 王铮 Ctr. Hepato-Biliaire-Hop. Paul- B. Assit. Pub.-Hpitaux de Paris UPRES EA3541 94804 Villejuif France

Background/Aims: As life expectancy in Hiv- HCV co- infected patients impro ves, end stage liver disease requiring.liver transplantation (lT) may become an emerging problem. We report the Paul Brousse Hospital experience of transplantat ion for end stage cirrhosis in Hiv- HCV co- infected patients. Methods: Seven consecutive Hiv- HCV co- infected patients were transplanted between December 1999 and December 2002 for end stage liver disease due to HCV. All patients were treated by hig.ly active antiretroviral therapy (HAART), Hiv plasma viral load was l and median CD4 lymphocyte count was 306 cells/mm3 (range, 10 3- 510) before lT. At the time of evaluation (March 2003), the median follow- up was 21 months (range, 4- 40). Results: Two patients died, 4 and 22 months, r espectively after lT. At the last biopsy, METAVIR score was staged F4 in two pat ients, F3 in two, and F1 in one. Microvesicular steatosis was noted in nearly al l patients. The ratio of mitochondrial to nuclear DnA was low in three of four p atients examined as compared with the amount of liver mtDnA found in eight Hiv- negative, HCV- infected controls (P=0.01). Conclusions: A significant defect i n the activity of the respiratory chain complex iv was noted in all five patient s studied. Mitochondrial hepatotoxicity and severe HCV recurrence occur in Hiv- HCV co- infected patients after lT.

关键词： Hiv-HCV 肝移植肝脏线粒体活组织学检查淋巴细胞计数呼吸链肝脏疾病晚期肝硬化肝细胞毒性脂肪变性

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Fung.l diversity notes 1151-1276:taxonomic and phylogenetic contributions on genera and species of fung.l taxa

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Fung.l Diversity 2020年第1期100卷 5-277页

作者： Kevin DHyde Yang Dong Rungtiwa Phookamsak Rajesh jeewon Djayarama Bhat EBgareth jones ning‑guo liu Pranami DAbeywickrama Ausana Mapook Deping Wei Rekhani HPerera Ishara SManawasinghe Dhandevi Pem Digvijayini Bundhun Anuruddha Karunarathna Anusha HEkanayaka Dan‑Feng Bao junfu li Milan CSamarakoon napalai Chaiwan Chuan‑gen lin Kunthida Phutthacharoen Sheng‑nan Zhang Indunil CSenanayake Ishani Dgoonasekara Kasun MThambug.la Chayanard Phukhamsakda Danushka STennakoon Hong‑Bo jiang jing Yang Ming Zeng naruemon Huanraluek jian‑Kui(jack)liu Subodini nWijesinghe Qing Tian Saowaluck Tibpromma Rashika SBrahmanage Saranyaphat Boonmee Shi‑Ke Huang Vinodhini Thiyagaraja Yong‑Zhong.lu Ruvishika Sjayawardena Wei Dong Er‑Fu Yang Sanjay KSingh Shiv Mohan Singh Shiwali Rana Sneha Slad garima Anand Bandarupalli Devadatha Mniranjan VVenkateswara Sarma Kare liimatainen 馻‑ Tuula niskanen Andy Overall Renato lúcio Mendes Alvarenga Tatiana Baptista gibertoni Walter PPfliegler EnikőHorváth Alexandra Imre Amanda lucia Alves Ana Carla da Silva Santos Patricia Vieira Tiago Timur SBulgakov Dhanushaka nWanasinghe Ali HBahkali Mingkwan Doilom Abdallah MElgorban Sajeewa SnMaharachchikumbura Kunhiraman CRajeshkumar Danny Haelewaters Peter EMortimer Qi Zhao Saisamorn lumyong jianchu Xu jun Sheng Key laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201YunnanChina State Key laboratory for Conservation and Utilization of Bio‑Resources in Yunnan Yunnan Agricultural UniversityKunming 650201China Yunnan Research Institute for local Plateau Agriculture and Industry Kunming 650201China Key laboratory for Agro‑biodiversity and Pest Control of Ministry of Education Yunnan Agricultural UniversityKunming 650201China Center of Excellence in Fung.l Research Mae Fah Luang UniversityChiang Rai 57100Thailand East and Central Asia Regional Office World Agroforestry Centre(ICRAF)Kunming 650201YunnanChina Centre for Mountain Futures(CMF) Kunming Institute of BotanyKunming 650201YunnanChina Mushroom Research Foundation 128 M.3 Ban Pa Deng T.Pa PaeA.Mae TaengChiang Mai 50150Thailand Department of Health Sciences Faculty of ScienceUniversity of MauritiusReduitMauritius no.128/1‑j Azad Co‑Op Housing SocietyCurcaGoa Velha 403108India Department of Entomology and Plant Pathology Faculty of AgricultureChiang Mai UniversityChiang Mai 50200Thailand Department of Botany and Microbiology College of ScienceKing Saud UniversityP.O.Box 2455Riyadh 11451Kingdom of Saudi Arabia School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Faculty of Agriculture National Resources and EnvironmentNaresuan UniversityPhitsanulok 65000Thailand Beijing Key laboratory of Environment Friendly Management on Fruit Diseases and Pests in north China Institute of Plant and Environment ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China Department of Soil Ecology UFZ-Helmholtz Centre for Environmental ResearchHalleGermany guizhou Key laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouChina Shenzhen Key laboratory of Microbial genetic Engineering College of Life Sciences and OceanographyShenzhen UniversityShenzhen 518060China Department of Plant M

Fung.l diversity notes is one of the important journal series of fung.l taxonomy that provide detailed descriptions and illustrations of new fung.l taxa,as well as providing new information of fung.l taxa *** article is the 11th contribution to the fung.l diversity notes series,in which 126 taxa distributed in two phyla,six classes,24 orders and 55 families are described and *** in this study were mainly collected from Italy by Erio Camporesi and also collected from China,India and Thailand,as well as in some other European,north American and South American *** described in the present study include two new families,12 new genera,82 new species,five new combinations and 25 new records on new hosts and new geographical distributions as well as sexual-asexual *** two new families are Eriomycetaceae(Dothideomycetes,family incertae sedis)and Fasciatisporaceae(Xylariales,Sordariomycetes).The twelve new genera comprise Bhagirathimyces(Phaeosphaeriaceae),Camporesiomyces(Tubeufiaceae),Eriocamporesia(Cryphonectriaceae),Eriomyces(Eriomycetaceae),neomonodictys(Pleurotheciaceae),Paraloratospora(Phaeosphaeriaceae),Paramonodictys(Parabambusicolaceae),Pseudoconlarium(Diaporthomycetidae,genus incertae sedis),Pseudomurilentithecium(lentitheciaceae),Setoapiospora(Muyocopronaceae),Srinivasanomyces(Vibrisseaceae)and Xenoanthostomella(Xylariales,genera incertae sedis).The 82 new species comprise Acremonium chiangraiense,Adustochaete nivea,Angustimassarina camporesii,Bhagirathimyces himalayensis,Brunneoclavispora camporesii,Camarosporidiella camporesii,Camporesiomyces mali,Camposporium appendiculatum,Camposporium multiseptatum,Camposporium septatum,Canalisporium aquaticium,Clonostachys eriocamporesiana,Clonostachys eriocamporesii,Colletotrichum hederiicola,Coniochaeta vineae,Conioscypha verrucosa,Cortinarius ainsworthii,Cortinarius aurae,Cortinarius britannicus,Cortinarius heatherae,Cortinarius scoticus,Cortinarius subsaniosus,Cytospora fusispor

关键词： 96 new taxa Agaricomycetes Ascomycota Basidiomycota Dothideomycetes lecanoromycetes leotiomycetes Pezizomycetes Phylogeny Saccharomycetes Taxonomy

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明尼苏达州Olmsted镇1976～1997年间自闭症的发病率:结果来自人口普查

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世界核心医学期刊文摘（儿科学分册） 2005年第5期1卷 37-37页

作者： Barbaresi W. j. Katusic S. K. Colligan R. C. 虎小毅 Div. of Devmtl. and Behav. Pediat. Mayo Clinic 200 First St SW Rochester MN 5 5905 United StatesDr.

Objective: To determine the incidence of autism among children in Olmsted Coun ty, Minnesota. Design: Through the Rochester Epidemiology Project, all inpatient and outpatient diagnoses are indexed for computerized retrieval. This computeri zed diagnostic index was used to identify children with any developmental disord er. A glossary of symptoms of autism was used to review medical and school recor ds of these children for symptoms consistent with Diagnostic and Statistical Man ual of Mental Disorders, Fourth Edition (DSM-iv) criteria for autistic disorder . Setting: Olmsted County, Minnesota. Subjects: All residents of Olmsted County 21 years or younger between 1976 and *** Outcome Measure: The incidence o f research-identified autism based on DSM-iv criteria for autistic Results: Th e age-adjusted incidence of researchidentified autism was 5.5 (95%confidence interval, 1.4-9.5) per 100000 chidren from 1980 to 1983 and 44.9 (95%confid ence interval, 32.9-56.9) from 1995 to 1997 (8.2-fold increase). This incre ase was confined to children younger than 10 years who were born after *** clusions: The incidence of researchidentified autism increased in Olmsted County from 1976 to 1997, with the increase occurring among young children after the i ntroduction of broader, more precise diagnostic criteria, increased availability of services, and increased awareness of autism. Although it is possible that un identified environmental factors have contributed to an increase in autism, the timing of the increase suggests that it may be due to improved awareness, change s in diagnostic criteria, and availability of services, leading to identificatio n of previously unrecognized young children with autism.

关键词：自闭症 Olmsted 明尼苏达州统计手册诊断标准罗切斯特环境因素心理疾病检测仪器

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