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Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat

作物学报:英文版 2023年第5期11卷 1512-1520页

作者： Caiyun Liu Wei Guo Yang Wang Bisheng Fu Jaroslav Doležel Ying Liu Wenling Zhai Mahmoud Said istván molnár Kateřina Holušová ruiqi Zhang Jizhong Wu Institute of Germplasm resources and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina College of Agronomy/JCIC-MCP/national Key Lab of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Experimental Botany Centre of the HanáRegion for Biotechnological and Agricultural ResearchŠlechtitelů31CZ-783671 Olomoucthe Czech Republic Field Crops research Institute Agricultural Research Centre9 Gamma StreetGiz112619 CairoEgypt Agricultural Institute Centre for Agricultural ResearchELKHMartonvásár 2462Hungary Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Zhongshan Biological Breeding Laboratory Nanjing 210014JiangsuChina

Wheat sharp eyespot, a stem disease caused by the soilborne fungus rhizoctonia cerealis van der Hoeven,has become a threat to wheat production worldwide. Exploiting resistance resources from wild relatives of wheat is a promising strategy for controlling this disease. In this study, a new wheat–Dasypyrum villosum T2DS·2V#4L translocation line in the background of Chinese Spring(CS) showed stable resistance to r. cerealis. Introgression of the T2DS·2V#4L chromosome into wheat cultivar Aikang 58 by backcrossing produced a marked increase in sharp eyespot resistance in nIL-T2DS·2V#4L in comparison with nILT2DS·2DL, and no detrimental effects of 2V#4L on agronomic traits were observed in the BC2F2, BC2F2:3,and BC2F2:4generations. Flow-sorted sequencing of 2V#4L yielded 384.3 Mb of assembled sequence, and8836 genes were predicted of which 6154 had orthologs in at least one of the 2AL, 2BL, and 2DL arms of CS, whereas 1549 genes were unique to 2V#4L. About 100,000 SnPs were detected in genes of 2V#4L and2DL in 10 sequenced bread wheat cultivars. A Kompetitive Allele Specific Polymerase chain reaction and30 conserved ortholog sequence markers were developed to trace the 2V#4L chromatin in wheat backgrounds. T2DS·2V#4L compensating translocation lines represent novel germplasm with sharp eyespot resistance and the markers will allow rapid detection in breeding programs.

关键词： Triticum aestivum rhizoctonia cerealis Dasypyrum villosum Flow sorting KASP

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虫生真菌非核糖体多肽活性产物生物合成潜力预测

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合成生物学 2021年第5期2卷 815-825页

作者：张礼文 istván molnár 徐玉泉中国农业科学院生物技术研究所北京100081 美国亚利桑那大学西南天然产物研究中心亚利桑那州图森85706美国

肉座菌目虫生真菌合成的非核糖体多肽类天然产物具有抗菌、杀虫、抗癌、调节免疫等生物活性,在临床和农业等领域有重要应用价值。近年来,随着真菌基因组测序数量的迅速增加、注释和分析工具的不断进步,人们发现真菌基因组中存在大量产... 详细信息

肉座菌目虫生真菌合成的非核糖体多肽类天然产物具有抗菌、杀虫、抗癌、调节免疫等生物活性,在临床和农业等领域有重要应用价值。近年来,随着真菌基因组测序数量的迅速增加、注释和分析工具的不断进步,人们发现真菌基因组中存在大量产物未知的天然产物合成基因。准确有效地预测这些基因的功能,筛选最有潜力合成新颖天然产物的基因簇,可以提高天然产物挖掘的效率。本研究选取40株肉座菌目虫生真菌基因组,系统分析了编码非核糖体多肽合成酶的基因及基因簇。基于隐马尔可夫模型,预测了445个模块型非核糖体多肽合成酶和1243个类非核糖体多肽合成酶;通过提取腺苷酰化结构域,构建序列相似性网络,以已知功能的非核糖体多肽合成酶作为标签,利用马尔可夫聚类算法,分析了非核糖体多肽产物的主要类别,发现了可能合成线性多肽、环缩肽、脂多肽、生物碱等新型结构化合物的基因簇。研究结果不仅揭示了肉座菌目虫生真菌合成非核糖体多肽活性产物的巨大潜力,而且为通过激活沉默基因簇挖掘新产物、利用合成生物学手段改造合成途径提供参考。

关键词：肉座菌目虫生真菌非核糖体多肽合成酶非核糖体多肽生物合成基因簇序列相似性网络分析

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Corrigendum to“Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat”[Crop J.11(2023)1512–1520]

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The Crop Journal 2023年第6期11卷 1949-1949页

作者： Caiyun Liu Wei Guo Yang Wang Bisheng Fu Jaroslav Dolezel Ying Liu Wenling Zhai Mahmoud Said istván molnár Katerina Holušová ruiqi Zhang Jizhong Wu Institute of Germplasm resources and Biotechnology/Jiangsu Provincial Key Laboratory of Agrobiology Jiangsu Academy of Agricultural SciencesNanjing 210014JiangsuChina College of Agronomy/JCIC-MCP/national Key Lab of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095JiangsuChina Institute of Experimental Botany Centre of the HanáRegion for Biotechnological and Agricultural ResearchŠlechtitelu 31CZ-783671 OlomoucCzech Republic Field Crops research Institute Agricultural Research Centre9 Gamma StreetGiza12619 CairoEgypt Agricultural Institute Centre for Agricultural ResearchELKHMartonvásár 2462Hungary Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina Zhongshan Biological Breeding Laboratory Nanjing 210014JiangsuChina

The authors regret to report some missing information in the authorship and associated changes of the *** page 1512,the author information reads:“Caiyun Liu a,Wei Guo a,Yang Wang b,Bisheng Fu a,Jaroslav Dolezel c,Ying Liu a,Wenling Zhai a,Mahmoud Said c,d,istván molnár c,d,e,Katerina Holušovác,ruiqi Zhang b,*,Jizhong Wu a,f,g,*”.It should be changed to“Caiyun Liu a,1,Wei Guo a,1,Yang Wang b,Bisheng Fu a,Jaroslav Dolezel c,Ying Liu a,Wenling Zhai a,Mahmoud Said c,d,istván molnár c,d,e,Katerina Holušovác,ruiqi Zhang b,*,Jizhong Wu a,f,g,*”.On page 1512 in the footnote,the following sentence should be added below the corresponding authors’information:1 These authors contributed equally to this *** correction has been updated *** authors would like to apologize for any inconvenience caused.

关键词： sharp correction bread

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Structural changes in the obese brain

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neural regeneration research 2024年第12期19卷 2561-2562页

作者： Anna r.r.Da Conceicao Marcelo n.n.Vieira Fernanda G.De Felice D’Or Institute for research and Education Rio de JaneiroBrazil Institute of Medical Biochemistry Leopoldo De Meis Federal University of Rio de JaneiroRio de JaneiroBrazil Centre for neuroscience Studies Department of Biomedical and Molecular Sciences&Department of PsychiatryQueen’s UniversityKingstonONCanada

Obesity represents a major global health concern,with widespread growing prevalence and severe consequences for the quality of life and life expectancy of affected individuals and for the economic burden of healthcare systems(Prospective Studies Collaboration,2009).Obesity is a well-established risk factor for a variety of conditions including metabolic,vascula r,and heart diseases,and seve ral types of cancer(Prospective Studies Collaboration,2009).

关键词： diseases consequences prevalence

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Aberrant adult neurogenesis in intractable epilepsy:can GABAergic progenitor transplantation normalize this process?

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neural regeneration research 2024年第7期19卷 1419-1420页

作者： Muhammad n.Arshad Janice r.naegele Department of Biology Wesleyan UniversityMiddletownCTUSA Department of neuroscience Tufts University School of MedicineBostonMAUSA

Temporal lobe epilepsy(TLE) is a common type of focal epilepsy characterized by seizure foci within the temporal *** surgical resection of the foci is an established and effective approach for controlling seizures,both temporal lobes cannot be removed,due to their prominent roles in learning and ***,seizures induce changes to the temporal lobes that contribute to hyperexcitability,including mossy fiber sprouting,astrogliosis.

关键词： epilepsy progenitor seizures

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Polyelectrolyte Complexes between Hyperbranched and Linear Polysaccharides: Fucoidan/Chitosan

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Chinese Journal of Polymer Science 2024年第4期42卷 468-479页

作者： Ekaterina r.Gasilova Daria n.Poshina Aleksandra O.Sitnikova natalia n.Saprykina Yury A.Skorik Institute of Macromolecular Compounds of the russian Academy of Sciences Bolshoi VO 31St.Petersburg199004Russian Federation

Polyelectrolyte complexes(PECs)of hyperbranched(HB)and linear polysaccharides are promising as more effective encapsulation agents compared to PECs formed by linear *** investigated the PECs between the HB anionic polysaccharide fucoidan(FUC)and the cationic linear polysaccharide chitosan(CS).The FUC had a molecular weight(MW)of 30×*** PECs were prepared in three solvents(water,0.01 and 0.1 mol/L acetic acid)with CS of MW of 15,110 and 170 kDa,and deacetylation degrees(DDA)of 70%and 97%.The structures of the PECs and the initial FUC were investigated by multi-angle static and dynamic light *** the FUC contained 18 wt%of—OSO3 groups and 5 wt%of uronic acid units,it was a“strong-weak”copolyanion,so the HB macromolecules of the FUC formed nanogel particles in 0.1 mol/L AcOH and open branched structures in water,as confirmed by the Kratky *** mixing the solutions of original components,the PEC structures underwent an equilibration period,the duration of which increased with the MW of *** the charge stoichiometry was approached,the PECs shrank;the fractal dimension approached unity,indicating the side-by-side packing of adjacent FUC branches with the help of *** aggregation in the vicinity of the charge compensation was hardly observed,as it occurred in a very narrow *** PEC content at theζ-potential inversion depended on solvents’pH and the DDA of *** the extreme case of core-shell PECs in 0.1 mol/L AcOH,obtained by mixing FUC nanogels with the solutions of high MW CS of 97%DDA,the protruding tails of CS formed a positively charged shell in the whole range of FUC content(10 wt%nning electron microscopy and atomic force microscopy images of dried samples were discussed in relation to the light scattering results.

关键词： Polyelectrolyte complexes Chitosan Hyperbranched fucoidan Light scattering

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A concise review on surface and structural modification of porous zeolite scaffold for enhanced hydrogen storage

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Chinese Journal of Chemical Engineering 2024年第6期70卷 33-53页

作者： B.A.Abdulkadir r.S.r.Mohd Zaki A.T.Abd Wahab S.n.Miskan Anh-Tam nguyen Dai-Viet n.Vo H.D.Setiabudi Centre for research in Advanced Fluid&Processes Universiti Malaysia Pahang Al-Sultan AbdullahLebuh Persiaran Tun Khalil YaakobGambangPahang 26300Malaysia Faculty of Chemical&Process Engineering Technology Universiti Malaysia Pahang Al-Sultan AbdullahLebuh Persiaran Tun Khalil YaakobGambangPahang 26300Malaysia Center of Excellence for Green Energy and Environmental nanomaterials(CE@GrEEn) Nguyen Tat Thanh University300A Nguyen Tat ThanhDistrict 4Ho Chi Minh City 755414Viet Nam

Investigating zeolites as hydrogen storage scaffolds is imperative due to their porous nature and favorable physicochemical ***,the storage capacity of the unmodified zeolites has been rather unsatisfactory(0.224%-1.082%(mass))compared to its modified ***,the contemporary focus on enhancing hydrogen storage capacities has led to significant attention towards the utilization of modified zeolites,with studies exploring surface modifications through physical and chemical treatments,as well as the integration of various active *** enhanced hydrogen storage properties of zeolites are attributed to the presence of aluminosilicates from alkaline and alkaline-earth metals,resulting in increased storage capacity through interactions with the charge density of these ***,there is a great demand to critically review their role such as well-defined topology,pore structure,good thermal stability,and tunable hydrophilicity in enhanced hydrogen *** article aimed to critically review the recent research findings based on modified zeolite performance for enhanced hydrogen *** of the factors affecting the hydrogen storage capacities of zeolites that can affect the rate of reaction and the stability of the adsorbent,like pressure,structure,and morphology were studied,and ***,future perspectives,recommendations,and directions for modified zeolites were discussed.

关键词： Zeolites Hydrogen storage Surface modification Adsorption Active metal

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Ion heat transport in electron cyclotron resonance heated L-mode plasma on the T-10 tokamak

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Plasma Science and Technology 2024年第4期26卷 52-60页

作者： V.A.KrUPIn M.r.nUrGALIEV A.r.nEMETS I.A.ZEMTSOV S.D.SUnTSOV T.B.MYALTOn D.S.SErGEEV n.A.SOLOVEV D.V.SArYCHEV D.V.rYJAKOV S.n.TUGArInOV n.n.nAUMEnKO national research Centre“Kurchatov Institute” Moscow 123182Russia Bauman Moscow State Technical University Moscow 105005Russia Institution“Project Center ITEr” Moscow 123098Russia JSC“SOLAr” Minsk 220034Belarus

Anomalous ion heat transport is analyzed in the T-10 tokamak plasma heated with electron cyclotron resonance heating(ECrH) in second-harmonic extra-ordinary mode. Predictive modeling with empirical scaling for Ohmical heat conductivity shows that in ECrH plasmas the calculated ion temperature could be overestimated, so an increase of anomalous ion heat transport is required. To study this effect two scans are presented: over the EC resonance position and over the ECrH power. The EC resonance position varies from the high-field side to the low-field side by variation of the toroidal magnetic field. The scan over the heating power is presented with on-axis and mixed ECrH regimes. Discharges with high anomalous ion heat transport are obtained in all considered regimes. In these discharges the power balance ion heat conductivity exceeds the neoclassical level by up to 10 times. The high ion heat transport regimes are distinguished by three parameters: the ratio Te/Ti, the normalized electron density gradient r/■, and the ion–ion collisionality νii~*. The combination of high Te/Ti, high νii~*, and r/■=6-10 results in values of normalized anomalous ion heat fluxes up to 10 times higher than in the low transport scenario.

关键词： tokamak L-mode electron cyclotron resonance heating ion heat transport

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Stability Analysis of DC Microgrid Clusters Based on Step-by-step System Matrix Building Algorithm

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 900-912页

作者： K.Jithin n.Mayadevi r.Hari Kumar V.P.Mini the Department of Electrical Engineering College of Engineering TrivandrumAPJ Abdul Kalam Technological University(APJAKTU)ThiruvananthapuramIndia

DC microgrids(DCMGs)are made up of a network of sources and loads that are connected by a number of power electronic converters(PECs).The increase in the number of these PECs instigates major concerns in system *** interconnecting the microgrids to form a cluster,the system stability must be *** paper proposes a novel stepby-step system matrix building(SMB)algorithm to update the system matrix of an existing DCMG cluster when a new microgrid is added to the cluster through a distribution *** stability of the individual DCMGs and the DCMG cluster is analyzed using the eigenvalue ***,the particle swarm optimization(PSO)algorithm is used to retune the controller gains if the newly formed cluster is not *** simulation of the DCMG cluster is carried out in MATLAB/Simulink environment to test the proposed *** results are also validated using the OP4510 real-time simulator(rTS).

关键词： Converter DC microgrid cluster eigenvalue interconnection particle swarm optimization system stability system matrix

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Automatic area estimation of algal blooms in water bodies from UAV images using texture analysis

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Water Science and Engineering 2024年第1期17卷 62-71页

作者： Ajmeria rahul Gundu Lokesh Siddhartha Goswami r.n.Ponnalagu radhika Sudha Department of Electrical and Electronics Engineering Birla Institute of Technology&SciencePilani Hyderabad CampusTelangana 500078India

Algal blooms,the spread of algae on the surface of water bodies,have adverse effects not only on aquatic ecosystems but also on human *** adverse effects of harmful algal blooms(HABs)necessitate a convenient solution for detection and *** aerial vehicles(UAVs)have recently emerged as a tool for algal bloom detection,efficiently providing on-demand images at high spatiotemporal *** study developed an image processing method for algal bloom area estimation from the aerial images(obtained from the internet)captured using *** a remote sensing method of HAB detection,analysis,and monitoring,a combination of histogram and texture analyses was used to efficiently estimate the area of *** features like entropy(using the Kullback-Leibler method)were emphasized with the aid of a gray-level co-occurrence *** results showed that the orthogonal images demonstrated fewer errors,and the morphological filter best detected algal blooms in real time,with a precision of 80%.This study provided efficient image processing approaches using on-board UAVs for HAB monitoring.

关键词： Algal bloom Image processing Texture analysis Histogram analysis Unmanned aerial vehicles

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