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Molecular Diagnosis of Sexual Differentiation Disorders in Burkina Faso

Journal of Biosciences and Medicines 2024年第3期12卷 1-15页

作者： Bazie Bapio Valérie Elvira Jean Télesphore Sawadogo Soumaïla Zohoncon Théodora Mahoukèdè Tall Ahmadou Bado Prosper Soubeiga Serge Théophile Ouattara Moussa Sorgho Abel Kiendrebeogo Touwendpoulimdé isabelle Yonli Albert Theophane Djigma Florencia Millogo/Traore Timongo Françoise Danielle Simpore Jacques Centre National de la Recherche Scientifique et Technologique (CNRST) Institut de Recherche en Sciences de la Santé (IRSS) Ouagadougou Burkina Faso Université Joseph KI-ZERBO Unité de Formation en Sciences de la Vie et de la Terre Laboratoire de Biologie Moléculaire et de Génétique (LABIOGENE) Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigonie (CERBA) Ouagadougou Burkina Faso Centre Hospitalier Universitaire Régional de Ouahigouya Ouagadougou Burkina Faso Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Sex development anomalies represent a group of congenital pathologies in which chromosomal, gonadal and anatomical sex differentiation is atypical. The aim of our study was to use molecular biology techniques to elucidate sex in cases of anomalies of sexual differentiation in Burkina Faso. This cross-sectional study took place from March 2023 to June 2023. Oral and blood samples were collected respectively using sterile swabs and stored on Swab Kits and NUCLEIcardTM (https://***/product-ranges/nucleic-card/) were used to determine gonosome profiles. Extraction was carried out using the DNA Swap solution Kit and the DNA IQ System Kit, and a PowerPlex? 21 kit (Promega) for amplification. The Applied Biosystems 9700 thermal cycler was used for PCR followed by 36 cm capillary electrophoresis in the Applied Biosystems 3130 prism sequencer. Sequence files were analyzed using GeneMapper IDX v. 3.2 software. Seven (07) patients were registered during the study period. There were 4 cases of XX DSD or 46, XX DSD and 3 cases of XY DSD or 46, XY DSD. The median age of our patients was 16 years. Civilian sex was male in 4 cases and female in 3. The most frequent reason for consultation was micropenis in 3 cases, followed by primary amenorrhea and sex ambiguity. There were 03 cases of discrepancy between genetic sex and civil sex. The accessibility of molecular diagnosis is little known to clinicians. XX DSDs or 46, XX DSDs were the most frequent (4/7) in our study. The problem facing this situation is early diagnosis to help prevent complications in Burkina Faso.

关键词： Gonosome DSD Abnormal Sexual Development Burkina Faso

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Potential effects of climate change on a marine invasion： The importance of current context

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Current Zoology 2012年第1期58卷 1-8页

作者： Côté, isabelle M. Green, Stephanie J. Department of Biological Sciences Simon Fraser University 8888 University Drive Burnaby BC Canada V5A 1S6

Species invasions threaten marine biodiversity globally. There is a concern that climate change is exacerbating this problem. Here, we examined some of the potential effects of warming water temperatures on the invasion of Western Atlantic habitats by a marine predator, the Indo-Pacific lionfish （Pterois volitans and P. miles）. We focussed on two temperature-dependent aspects of lionfish life-history and behaviour： pelagic larval duration, because of its link to dispersal potential, and prey consump- tion rate, because it is an important determinant of the impacts of lionfish on native prey. Using models derived from fundamental metabolic theory, we predict that the length of time spent by lionfish in the plankton in early life should decrease with warming temperatures, with a concomitant reduction in potential dispersal distance. Although the uncertainty around change in dispersal distances is large, predicted reductions are, on average, more than an order of magnitude smaller than the current rate of range expansion of lionfisli in the Caribbean. Nevertheless, because shorter pelagic larval duration has the potential to increase local re- tention of larvae, local lionfish management will become increasingly important under projected climate change. Increasing tem- perature is also expected to worsen the current imbalance between rates of prey consumption by lionfish and biomass production by their prey, leading to a heightened decline in native reef fish biomass. However, the magnitude of climate-induced decline is predicted to be minor compared to the effect of current rates of lionfish population increases （and hence overall prey consumption rates） on invaded reefs. Placing the predicted effects of climate change in the current context thus reveals that, at least for the lionfish invasion, the threat is clear and present, rather than future [Current Zoology 58 （1）： 1-8, 2012].

关键词： Non-indigenous species Coral reefs Dispersal Ecological impacts of invasion

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Laser Measurements in High Speed Compressors for Rotor-Stator Interaction Analysis

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Journal of Thermal Science 2003年第4期12卷 310-317,309页

作者： Xavier Ottavy isabelle Trébinjac André Vouillarmet David Arnaud Laboratoire de Mecanique des Fluides et d'Acoustique UMR CNRS 5509/ECL/UCB Lyon I Ecole Centrale de Lyon 36 Avenue Guy de Collongue 69134 ECULLY Cedex France

This paper deals with the experimental quantification of the unsteady effects of the interactions between rotor and stator rows in high speed compressors. Due to the fact that the levels of the periodic fluctuations arising from the unsteady interaction may be low compared with the random fluctuations arising from the measurement uncertainties, it is crucial to minimize the errors inherent to the used technique. The first part of the paper concentrates on technical details relative to the experimental process. The second part is devoted to the data post-processing. Two tools for analysing the rotor-stator interactions are presented. The first tool is based on a decomposition of the flow field which was initially introduced to solve numerical problems when attempting to calculate the flow field in a multi-row configuration. The second tool is based on a spectral analysis of the signal, that qualifies the interaction in a sense of circumferential spinning lobes. Experimental results obtained within both an axial and a centrifugal high speed compressors are used to illustrate the data processing. In both cases, the effects of the unsteady interaction are quantified.

关键词： laser anemometry high speed compressor rotor-stator interaction.

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Terrorist attacks:cutaneous patterns of gunshot and secondary blast injuries

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Forensic Sciences Research 2020年第3期5卷 208-213页

作者： Yann Delannoy isabelle Plu isabelle Sec Tania Delabarde Marc Taccoen Antoine Tracqui Bertrand Ludes Institut Medico-Legal de Paris ParisFrance CHU de LILLE LilleFrance Sorbonne Universite ParisFrance Unite Medico Judiciaire Hotel DieuAPHPParisFrance Service de medecine legale CHRU Besanc¸on—Hopital Saint-JacquesBesanconFrance Paris University CNRS FRE 2029ParisFrance

Terrorist attacks have been on the *** the recent terrorist attacks in France,terrorists perpetrated their acts using weapons of war,as well as explosive *** two modes of action,when combined,can create skin lesions with similar macroscopic appearances,which can sometimes go unnoticed because of body fragmentation.A total of 68 autopsies,83 external examinations,140 standard radiographic examinations,and 49 computed tomography(CT)scans were performed over 7 days during the 2015 terrorist attacks in *** were injured by firearms and shrapnel-like *** analysed the clinical findings for the secondary blast cutaneous lesions from the explosive devices and compared these lesions with ballistic-related lesions to highlight that patterns can be macroscopically similar on external *** blast injuries are characterised by penetrating trauma associated with materials added to explosive systems that are propelled by explosive air *** injuries are caused most often by small,shrapnel-like metallic objects,such as nails and *** causes ballistic-type injuries that must be recognised and distinguished from those caused by firearm *** between these lesions is very difficult when using conventional criteria(size,shape,number and distribution on the body)with only external examination of *** is why the particularities of these lesions must be further illustrated and then confirmed by complete autopsies and radiological and anatomopathological examinations.

关键词： Forensic sciences forensic medicine blast injuries explosive agents suicide gunshot wounds

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Distances between Elements of a Semigroup and Estimates for Derivatives

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Acta Mathematica Sinica,English Series 2010年第12期26卷 2239-2254页

作者： Zohra BENDOUAD isabelle CHALENDAR Jean ESTERLE Jonathan R.PARTINGTON Université Amar Telidji Laboratoire de Sciences Economiques et Sciences de Gestion Université de Lyon CNRS Université Lyon 1 Institut Camille Jordan L.A.B.A. G Université Bordeaux 1 351 cours de la Libération School of Mathematics University of Leeds

This paper is concerned first with the behaviour of differences T（t） - T（s） near the origin, where （T（t）） is a semigroup of operators on a Banach space, defined either on the positive real line or a sector in the right half-plane （in which case it is assumed analytic）. For the non-quasinilpotent case extensions of results in the published literature are provided, with best possible constants; in the case of quasinilpotent semigroups on the half-plane, it is shown that, in general, differences such as T（t） -T（2t） have norm approaching 2 near the origin. The techniques given enable one to derive estimates of other functions of the generator of the semigroup; in particular, conditions are given on the derivatives near the origin to guarantee that the semigroup generates a unital algebra and has bounded generator.

关键词： Semigroup of operators analytic semigroup Banach algebras non-quasinilpotent quasinilpotent

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Time-dependent radiolytic yields at room temperature and temperature-dependent absorption spectra of the solvated electrons in polyols

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Nuclear Science and Techniques 2007年第1期18卷 2-9页

作者： Mingzhang LIN Mehran MOSTAFAVI Yusa MUROYA isabelle LAMPRE Yosuke KATSUMURA Department of Nuclear Engineering and Management School of Engineering The University of Tokyo Hongo 7-3-1 Bunkyo-ku Tokyo 113-8656 Japan Laboratoire de Chimie Physique/ELYSE UMR 8000 CNRS / Université Paris-Sud Centre d＇Orsay Bat. 349 91405 Orsay Cedex France Nuclear Professional School School of Engineering The University of Tokyo 2-22 Shirakata Shirane Tokaimura Naka gun Ibaraki 319-1188 Japan

The molar extinction coefficients at the absorption maximum of the solvated electron spectrum have been evaluated to be 900,970,and 1000 mol^(-1).m^2 for 1,2-ethanediol (12ED),1,2-propanediol (12PD),and 1,3-propanediol (13PD),*** values are two-third or three-fourth of the value usually reported in the published report. Picosecond pulse radiolysis studies have aided in depicting the radiolytic yield of the solvated electron in these sol- vents as a function of time from picosecond to *** radiolytic yield in these viscous solvents is found to be strongly different from that of the water *** temperature dependent absorption spectra of the solvated electron in 12ED,12PD,and 13PD have been also *** all the three solvents,the optical spectra shift to the red with increasing *** the shape of the spectra does not change in 13PD,a widening on the blue side of the absorption band is observed in 12ED and 12PD at elevated temperatures.

关键词：多羟基化合物溶剂化电子时间相关性辐射分解室温温度相关性吸收谱

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Local Dispersive and Strichartz Estimates for the Schr?dinger Operator on the Heisenberg Group

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Communications in Mathematical Research 2023年第1期39卷 1-35页

作者： Hajer Bahouri isabelle Gallagher CNRS&Sorbonne Universite Laboratoire Jacques-Louis Lions(LJLL)UMR 75984 Place Jussieu 75005 ParisFrance DMA Ecole Normale SuperieureCNRSPSL Research University75005 ParisFrance UFR de Mathematiques Universitéde Paris75013 ParisFrance

It was proved by Bahouri et al.[9]that the Schrodinger equation on the Heisenberg group H^(d),involving the sublaplacian,is an example of a totally non-dispersive evolution equation:for this reason global dispersive estimates cannot *** paper aims at establishing local dispersive estimates on H^(d) for the linear Schrodinger equation,by a refined study of the Schrodinger ker-nel St on H^(d).The sharpness of these estimates is discussed through several *** approach,based on the explicit formula of the heat kernel on H^(d) derived by Gaveau[19],is achieved by combining complex analysis and Fourier-Heisenberg *** a by-product of our results we establish local Stri-chartz estimates and prove that the kernel St concentrates on quantized hori-zontal hyperplanes of H^(d).

关键词： Heisenberg group Schrodinger equation dispersive estimates Strichartz estimates

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Neuregulin 1 isoforms could be an effective therapeutic candidate to promote peripheral nerve regeneration

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Neural Regeneration Research 2014年第12期9卷 1183-1185页

作者： Giovanna Gambarotta Giulia Ronchi Stefano Geuna isabelle Perroteau Department of Clinical and Biological Sciences University of Turin Neuroscience Institute of Turin (NIT) University of Turin Neuroscience Institute of the "Cavalieri Ottolenghi" Foundation(NICO) University of Turin

Traumatic injuries of peripheral nerves represent common casualties and their social impact is considerably high. Although peripheral nerves retain a good regeneration potential, the clinical outcome after nerve lesion is far from being satisfactory and functional recovery is almost never complete, especially in the case of large nerve defects, that result in loss or diminished sensitivity and/or motor activity of the innervated target organs. Therefore, to improve the outcome after nerve damage, or in peripheral neuropathies, there is a need for further research in nerve repair and regeneration to identify factors that promote axonal regrowth, remvelination and target reinnervation.

关键词： NRG BACE Neuregulin 1 isoforms could be an effective therapeutic candidate to promote peripheral nerve regeneration TACE

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Deciphering the role of monosaccharides during phage infection of Staphylococcus aureus

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Nano Research 2022年第10期15卷 9234-9242页

作者： Baptiste Arbez Marion Gardette Christophe Gantzer Neus Vilà isabelle Bertrand Sofiane El-Kirat-Chatel Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement(LCPME) CNRSUniversité de Lorraine405 rue de Vandoeuvre54602 Villers-lès-NancyFrance

As phages are extensively investigated as novel therapy tools but also as transfer agents for antibiotic resistance genes,thorough understanding of phage–host interactions becomes *** for phage infection is its adhesion to the host ***,we used atomic force microscopy-based single-particle force spectroscopy with phage-decorated tips to decipher the adhesion of phage 187 on living Staphylococcus aureus *** found that addition of free N-acetyl-Dglucosamine was able to decrease phage adhesion,suggesting that this monosaccharide plays major role in phage 187 infection of ***,phage 187 adhesion on monosaccharide-coated model surfaces combined with plaque forming unit counts suggested that a direct link can be established between the propensity to bind to a saccharide and the capability of the latter to inhibit phage *** a nanoscale level,single-particle force spectroscopy was successfully used to identify a major receptor required for phage 187 infection of *** but also evidenced that this receptor was responsible for phage adhesion on *** work demonstrates that single-particle force spectroscopy is a powerful platform to screen and predict the molecular target of phages on their host surfaces.

关键词： atomic force microscopy single-particle force spectroscopy phage 187 Staphylococcus aureus phage-bacteria interactions

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Increasing the number of discrete character states for continuous characters generates well-resolved trees that do not reflect phylogeny

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Integrative Zoology 2014年第4期9卷 531-541页

作者： Jérémie BARDIN isabelle ROUGET Margaret Mary YACOBUCCI Fabrizio CECCA Sorbonne University Pierre-and-Marie-Curie University(UPMC-P6)ParisFrance National Museum of Natural History(MNHN) ParisFrance The National Center for Scientific Research(CNRS) ParisFrance Department of Geology Bowling Green State UniversityBowling GreenOhioUSA

Since the introduction of the cladistic method in systematics,continuous characters have been integrated into analyses but no methods for their treatment have received unanimous *** methods require a large number of character states to discretise continuous characters in order to keep the maximum level of information about taxa differences within the coding *** objective was to assess the impact of increasing the character state number on the outcomes of phylogenetic *** of a variety of simulated datasets shows that these methods for coding continuous characters can lead to the generation of well-resolved trees that do not reflect a phylogenetic *** call this phenomenon the flattening of the tree-length distribution;it is influenced by both the relative quantity of continuous characters in relation to discrete characters,and the number of characters in relation to the number of *** tests provide a method to avoid this potential bias.

关键词： cladistics continuous character gap weighting phylogeny

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