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Optimization of the Production of Biogas in Anaerobic Digestion by the Addition of Hydrolytic Microorganisms

Journal of Environmental Science and Engineering(A) 2022年第1期11卷 8-12页

作者： María Montes Melissa Cervantes Alejandra Vásquez Víctor Aguilar Pedro García isabel del castillo Department of Environmental Engineering Institute for EngineeringUniversity of VeracruzBoca del RioVer.94294Mexico Department Chemical Engineering Faculty of Chemical SciencesUniversity of VeracruzBoca del RioVer.94294Mexico Department of Administration Open Teaching SystemUniversity of VeracruzBoca del RioVer.94294Mexico Department Sanitary Engineering E.T.S.I.Civil EngineeringPolytechnic University of MadridMadrid28040Spain

It is well known that fossil fuels are one of the major pollutants that contribute to climate change and there is a need to develop alternatives to their *** option is the use of a renewable fuel such as biogas obtained from the breaking down of organic matter in the anaerobic digestion *** biogas is cleaned up to a certain quality,it can substitute the use of natural ***,this slow process requires optimization to increase the amount of *** are chemical,physical and biological treatments for organic matter to increase the amount and quality of *** the former research,a biological treatment was tested by adding a selection of microorganisms that showed hydrolytic activity to an anaerobic digester for its ***,a selection of microorganisms with the ability to grow in fresh sewage sludge as only nutrient was ***,the lipolytic,cellulolytic and PA(Proteolytic Activity)of microorganisms was tested using selective growth ***,10 samples with the greatest hydrolytic activity were used to evaluate their effects in the increasing of biogas production in the anaerobic *** results showed that 83%of the microorganisms with the ability to grow in sewage sludge were proteolytic,while the percentage of lipolytic and cellulolytic microorganisms was ***,it was found that proteolytic microorganisms are the ones that increase biogas yield in a maximum of 80%with a COD(Chemical Oxygen Demand)reduction of 58%.

关键词： Hydrolytic microorganisms biogas anaerobic digestion

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Nuptial gifts and female fecundity in the neotropical katydid Conocephalus ictus （Orthoptera： Tettigonidae）

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Insect Science 2015年第1期22卷 106-110页

作者： isabel Ortiz-Jimenez Raul Cueva del castillo Laboratorio de Ecologia UBIPRO UNAM FES Iztacala M6xico Edo. Mexico M6xico

In general, female fitness is greatly increased in gift-giving insects. In katydids, this nuptial gift consists of a gelatinous mass produced by accessory glands： the spermato- phylax, which is attached to the ampulla. During mating, males of the neotropical katydid Conocephalus ictus transfer a spermatophylax that is ingested by the females. Fecundity, egg-laying rate and longevity were higher in females that consumed the spermatophylax than in those that did not. Also, female receptivity turned off after mating. Females actively rejected other males by hitting them with their forelegs and moving away. Their refractory period lasted as long as 17 d. Only a few females accepted a 2nd mating and died a few days later. In C. ictus, spermatophylax consumption can be beneficial for both males and females. On one hand, the compounds in the spermatophylax or the ejaculate could prevent or delay females from copulating with rivals, thus avoiding sperm competition. On the other hand, such compounds can improve the females＇ opportunity to increase their lifespan and fecundity. Moreover, a rise in egg-laying rate may lower the risk of female prereproductive death caused by rapid oviposition. In any case, the boost in female egg laying might also be beneficial for males because their number of offspring increases.

关键词： female fitness katydid neotropic spermatophylax

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Starburst amacrine cells,involved in visual motion perception,lose their synaptic input from dopaminergic amacrine cells and degenerate in Parkinson’s disease patients

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Translational Neurodegeneration 2023年第1期12卷 638-653页

作者： Xavier Sánchez-Sáez isabel Ortuño-Lizarán Carla Sánchez-castillo Pedro Lax Nicolás Cuenca Department of Physiology Genetics and MicrobiologyUniversity of AlicanteSan Vicente del RaspeigSpain Alicante Institute for Health and Biomedi-cal Research(ISABIAL) AlicanteSpain Ramón Margalef Institute University of AlicanteSan Vicente del RaspeigSpain.

Background The main clinical symptoms characteristic of Parkinson’s disease(PD)are bradykinesia,tremor,and other motor ***,non-motor symptoms,such as visual disturbances,can be identified at early stages of the *** of these symptoms is the impairment of visual motion ***,we sought to determine if the starburst amacrine cells,which are the main cellular type involved in motion direction selectivity,are degenerated in PD and if the dopaminergic system is related to this *** Human eyes from control(n=10)and PD(n=9)donors were available for this *** immunohistochemistry and confocal microscopy,we quantified starburst amacrine cell density(choline acetyltransferase[ChAT]-positive cells)and the relationship between these cells and dopaminergic amacrine cells(tyrosine hydroxylase-positive cells and vesicular monoamine transporter-2-positive presynapses)in cross-sections and wholemount *** First,we found two different ChAT amacrine populations in the human retina that presented different ChAT immunoreactivity intensity and different expression of calcium-binding *** populations are affected in PD and their density is reduced compared to ***,we report,for the first time,synaptic contacts between dopaminergic amacrine cells and ChAT-positive cells in the human *** found that,in PD retinas,there is a reduction of the dopaminergic synaptic contacts into ChAT *** Taken together,this work indicates degeneration of starburst amacrine cells in PD related to dopaminergic degeneration and that dopaminergic amacrine cells could modulate the function of starburst amacrine *** motion perception circuitries are affected in PD,their assessment using visual tests could provide new insights into the diagnosis of PD.

关键词： Parkinson’s disease Retinal neurodegeneration Human retina ChAT amacrine cells Dopaminergic amacrine cells

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Medicinal plants and natural products as neuroprotective agents in age-related macular degeneration

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Neural Regeneration Research 2020年第12期15卷 2207-2216页

作者： Francisco Bosch-Morell Victoria Villagrasa Teresa Ortega Nuria Acero Dolores Muñoz-Mingarro MEugenia González-Rosende Encarna castillo MAmparo Sanahuja Pilar Soriano isabel Martínez-Solís Biomedical Sciences Institute Universidad Cardenal Herrera-CEUCEU UniversitiesAlfara del PatriarcaValenciaSpain Department of Biomedical Sciences Faculty of Health SciencesUniversidad Cardenal Herrera-CEUCEU UniversitiesAlfara del PatriarcaValenciaSpain Department of Pharmacy Faculty of Health SciencesUniversidad Cardenal Herrera-CEUCEU UniversitiesAlfara del PatriarcaValenciaSpain Department of Pharmacology Pharmacognosy and BotanyUniversidad Complutense de MadridMadridSpain Department of Pharmaceutical and Health Sciences Faculty of PharmacyUniversidad San Pablo-CEUCEU UniversitiesMadridSpain Department of Chemistry and Biochemistry Faculty of PharmacyUniversidad San Pablo-CEUCEU UniversitiesMadridSpain ICBiBE-Botanical Garden University of ValenciaValenciaSpain

The retina may suffer neurodegenerative damages,as other tissues of the central nervous system do,and serious eye diseases may *** of them is age-related macular degeneration,which causes progressive loss of vision due to retina *** of age-related macular degeneration focuses on antioxidant agents and anti-vascular endothelial growth factor compounds,among others,that prevent/diminish oxidative stress and reduce neovascularisation *** phytochemicals,medicinal plants and/or plant-diet supplements might be a useful adjunct in prevention or treatment of age-related macular degeneration owing to their antioxidant and anti-vascular endothelial growth factor *** review article presents the most investigated plants and natural products in relation to age-related macular degeneration,such as saffron,ginkgo,bilberry and blueberry,curcuma or turmeric,carotenoids,polyphenols,and vitamins C and *** study provides up-to-date information on the effects,treatments,safety and efficiency of these phytotherapy products.

关键词： age-related macular degeneration bilberry blueberry curcuma carotenoids ginkgo polyphenols saffron vitamins

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Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer

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Cancer Biology & Medicine 2020年第2期17卷 444-457页

作者： Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima Navarro Patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria Ana Belen Enguita Maria Cebollero Amparo Benito isabel Alemany Carolina del castillo Ricardo Ramos Ana Ramirez de Molina Enrique Casado Maria Sereno Ana Ramirez de Molina1 Enrique Casado5Maria Sereno5 XIMDEA Food InstituteCEI UAM+CSICMadrid 28702Spain 12 de Octubre University Hospital Madrid 28041Spain Gregorio Maranon University Hospital Madrid 28009Spain Ramon y Cajal University Hospital Madrid 28034Spain Infanta Sofia University Hospital San Sebastian De Los ReyesMadrid 28702Spain Fundacion Alcorcon University Hospital AlcorconMadrid 28922Spain Prlncipe de Asturias University Hospital Alcala De HenaresMadrid 28805Spain La Paz University Hospital Madrid 28046Spain University Hospital of Torrejon Torrejon De ArdozMadrid 28850Spain Clinico San Carlos University Hospital Madrid 28040Spain Parque Cientifico de Madrid Foundation Madrid 28049Spain

Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with *** are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the *** this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular ***:In the first step,we conducted a clinical-pathological characterization of the ***,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the ***:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of *** obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)***,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.

关键词： Long-term survivors non-small cell lung cancer surfactant proteins

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Description of the Health Status of Colombian Indigenous Communities Through Basic Laboratory Tests

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Journal of Life Sciences 2014年第9期8卷 789-793页

作者： Martha castillo Ana Lucia Oliveros Ana isabel Mora Faculty of Health Sciences Universidad Colegio Mayor de Cundinamarca Calle 28 No. 5B-02 Bogot6 D.C. 110911 Colombia

Colombia enjoys considerable ethnic diversity, although its indigenous population, part of the world＇s shared cultural heritage has been reduced by various factors, including displacement, poverty, and poor access to timely and quality healthcare. The present study is focused on diagnosing health problems, particularly anemia, through a description of hematological findings amongst men and woman aged 18 to 50 years, living at various altitudes, and belonging to several different indigenous communities： the Huitoto and Embera of Caqueta, Florencia; the Born, Muinane, Okaina, Huitoto communities of La Chorrera, Amazonas; and the Yanaconas of Bogota, D.C. Tests performed included automated hemogram tests, ferritin, c-reactive protein, and parasitological assessment to correlate the presence of anemia and parasitism. The authors used a quantitative and transversal, structured, non-experimental, correlational, stratified sample design with 105 samples （51 men, 54 women）. The results showed that 7.8% （4/51） of the men had anemia, compared with 29.6% （16/54） of the women--more than triple the incidence of the male population. Moreover, we found a higher incidence of anemia in rural areas, where the majority of the country＇s indigenous populations live. In the parasitological study, 87.7% （50/57） of the participants tested positive for intestinal parasites. These findings are expected to help authorities implement more effective responses to health issues in these communities.

关键词： Embera Bora Muinane Huitoto Okaina native Americans anemia intestinal parasitism.

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Association of Thalassemia and Trait, Sickle Cell and Trait and Haemoglobin C with Iron-Deficiency Anemia in Colombians of African Descent

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Journal of Life Sciences 2014年第11期8卷 861-864页

作者： Martha castillo Ana Lucia Oliveros Ana isabel Mora Bautista Faculty of Health Sciences Universidad Colegio Mayor de Cundinamarca Calle 28 No. 5B-02 Bogot6 D.C. 110911 Colombia

The conditions of poverty and violence in Colombia have increased the displacement of people of African descent. The research group concerned with the health of these communities has performed research to find the association of alterations in the hemoglobin molecule with the presence of iron deficiency anemia. This research was performed with the support of the epidemiological public health laboratory. The objective of the study is the association of sickle cell disease and sickle cell trait, and the presence of hemoglobin C and its trait, with iron deficiency anemia in Afro-Colombians between 18 and 50 years of age, by investigation of hemograms, peripheral blood study, serum ferritin, soluble receptor for transferrin （sTfR） and index of sTfR, and hemoglobin electrophoresis. This research is a descriptive, quantitative, transverse, structured, non-experimental and correlational study, with a total of 56 samples （10 men, 46 women）. The results showed that ferritin in men had normal values, compared to 32% （15/46） of women who had low levels of ferritin. Men＇s sTfR levels were normal, while in women 2% （1/46） had iron deficiency in stage I, 41% （19/46） iron deficiency in stage If, and 9% （4/46） possibly without anemia iron deficiency. In hemoglobin electrophoresis, 84% （46/56） of the population presents hemoglobin A, 4% （2/56） increased hemoglobin A2 and fetal hemoglobin related to a possible thalassemia trait, 11% （6/56） had hemoglobin AS related to sickle cell trait, and 2% （1/56） show hemoglobin AC, related to hemoglobin AC trait. This project supports investigative and social projects, and also contributes to the improvement of the quality of life and dissemination of good health practices in these communities.

关键词： Afro-descendant Hemoglobinopathies iron deficiency anemia trait.

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Utility of C-Reactive Protein and Procalcitonin for Detecting Bloodstream Infection in Patients with HIV/AIDS

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World Journal of AIDS 2014年第3期4卷 287-292页

作者： Arianna castillo Marshall Tersilia García Castellanos isabel Martínez Motas Daniel Salazar Rodriguez María Eugenia Toledo Romaní Jorge Pérez ávila Clinical Laboratory International Health Center La Pradera La Havana Cuba Department of Clinical Microbiology Institute of Tropical Medicine “Pedro Kourí” La Havana Cuba Epidemiology Research Department Institute of Tropical Medicine “Pedro Kourí” La Havana Cuba Institute of Tropical Medicine “Pedro Kourí” La Havana Cuba Medicine Faculty Latin-American’s School La Havana Cuba

Human immunodeficiency virus (HIV) infected persons, are at high risk for developing a bloodstream infection. In order to evaluate the usefulness of C-reactive protein (CRP) and procalcitonin (PTC) in the detection of bloodstream infection in HIV, a case-control study was conducted from February to December 2012. PCT and CPR levels were measured in 2 groups. PCT concentrations were measured by the VIDAS®?Brahms PCT assay, and CRP concentrations were determined by CRP latex. Values were calculated for both biomarkers and discriminative ability of PCT and CRP was analyzed using ROC curves. There were no significant differences between the study group and the control groups with respect to CRP levels. However, they were much higher PCT levels in patients with bacteremia. PCT showed greater discriminating ability compared to CRP, and proved to be a valuable tool for the detection of systemic bacterial infections in HIV infected patients.

关键词： Acute Renal Injury Surgical Hemostasis Absorbable Implants Inflammation Biocompatible

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Effect of the Substitution of Sand by Rubber of Waste Tires on the Mechanical Properties of Hydraulic Concrete and Exposure to Gamma Radiation

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Journal of Minerals and Materials Characterization and Engineering 2021年第3期9卷 245-256页

作者： Jesús M. Colín de la Cruz Carmen Gabriela Guzmán Fermín castillo Mejía Benjamin Leal Acevedo Osvaldo Flores Cedillo isabel Gamboa de Buen Arturo Molina Ocampo Horacio Martínez Valencia Facultad de Ciencias Químicas e Ingeniería Universidad Autónoma del Estado de Morelos Cuernavaca México Laboratorio de Espectroscopia Instituto de Ciencias Físicas Universidad Nacional Autónoma de México Cuernavaca México Instituto de Ciencias Nucleares Universidad Nacional Autónoma de México México City México Centro de Investigación en Ingeniería y Ciencias Aplicadas UAEM Cuernavaca México

For a long time and until now, rubber is the most used material for the manufacture of tires for motor vehicles. Unfortunately, once the tire meets its life cycle, the remaining rubber cannot be recycled, so the tires are discarded in collection centers and often in clandestine dumps. This represents a serious environmental problem because, in one case, these waste tires become breeding grounds for insects and wildlife that is harmful to humans. In the second case, the tires are burned, releasing highly damaging gases into the atmosphere. On the other hand, concrete is worldwide the construction material par excellence. It is basically composed of cement, gravel and sand. Mixing these three components in different proportions, their mechanical strength in compression can be increased. However, due to its fragile nature, concrete, once a crack is formed, it rapidly advances by fragmenting the material and producing its rapid collapse. In the present work, in order contribute to the care of the environment as well as to modify the fracture mode of the concrete, rubber particles obtained from waste tires were used as sand substitute in hydraulic concrete. In addition, rubber modified samples concrete were lately exposed to 70 kGy of gamma radiation in order to study the effects of this radiation on the mechanical deformation of concrete. The results showed a decrease in the mechanical properties of the concrete with rubber particles with respect to the traditional concrete itself. However, such decreases were offset by the fact that samples with rubber addition do not collapses as fast as the free rubber samples. The acquired data pave the way for research with great benefits, such as the use of recycled tires in concrete for its fracture mode modification in a beneficial way, as well as a possible decrease in the cost of concrete.

关键词： Hydraulic Concrete Scrap Tire Rubber Strength Gamma Radiation Mechanical Properties

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Correction:Starburst amacrine cells,involved in visual motion perception,lose their synaptic input from dopaminergic amacrine cells and degenerate in Parkinson’s disease patients

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Translational Neurodegeneration 2023年第1期12卷 574-574页

作者： Xavier Sánchez-Sáez† isabel Ortuño-Lizarán† Carla Sánchez-castillo Pedro Lax Nicolás Cuenca Department of Physiology Genetics and MicrobiologyUniversity of AlicanteSan Vicente del RaspeigSpain Alicante Institute for Health and Biomedical Research(ISABIAL) AlicanteSpain Ramón Margalef Institute University of AlicanteSan Vicente del RaspeigSpain

Correction:Translational Neurodegeneration(2023)12:17(2023)https://***/10.1186/s40035-023-00348-y Following publication of the original article[1],the authors reported an error in the article *** publication of the original article[1],the authors reported an error in the article title.“Lose”was mistakenly typed as“loose”in the original *** correct title should read:Starburst amacrine cells,involved in visual motion perception,lose their synaptic input from dopaminergic amacrine cells and degenerate in Parkinson’s disease patients.

关键词： degenerate motion loose

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